FP1220436P/CB CN 202210568686.9 2022-05-24 Juventas Unicare Pharmaceutical (Beijing) Co., Ltd. BISPECIFIC CHIMERIC ANTIGEN RECEPTOR TARGETING BCMA-CD19 AND APPLICATION THEREOF 40 8 AA PAT REGION 1..8 note BCMA VH CDR1 source 1..8 mol_type protein organism synthetic construct GFSLSTYH 7 AA PAT REGION 1..7 note BCMA VH CDR2 source 1..7 mol_type protein organism synthetic construct ISSSGST 10 AA PAT REGION 1..10 note BCMA VH CDR3 source 1..10 mol_type protein organism synthetic construct ARDLDYVIDL 7 AA PAT REGION 1..7 note BCMA VL CDR1 source 1..7 mol_type protein organism synthetic construct PSVYNNY 000 11 AA PAT REGION 1..11 note BCMA VL CDR3 source 1..11 mol_type protein organism synthetic construct AGTYVSGDRRA 8 AA PAT REGION 1..8 note CD19 VH CDR1 source 1..8 mol_type protein organism synthetic construct GYAFSSYW 8 AA PAT REGION 1..8 note CD19 VH CDR2 source 1..8 mol_type protein organism synthetic construct IYPGDGDT 15 AA PAT REGION 1..15 note CD19 VH CDR3 source 1..15 mol_type protein organism synthetic construct ARKTISSVVDFYFDY 6 AA PAT REGION 1..6 note CD19 VL CDR1 source 1..6 mol_type protein organism synthetic construct QNVGTN 000 9 AA PAT REGION 1..9 note CD19 VL CDR3 source 1..9 mol_type protein organism synthetic construct QQYNRYPYT 116 AA PAT REGION 1..116 note BCMA VH source 1..116 mol_type protein organism synthetic construct EVQLVESGGGLVQPGGSLRLSCTASGFSLSTYHMTWVRQAPGKGLEWIGVISSSGSTYYASWAKGRFTISRDNSKNTVYLQMNSLRAEDTAVYFCARDLDYVIDLWGPGTLVTVSS 110 AA PAT REGION 1..110 note BCMA VL source 1..110 mol_type protein organism synthetic construct EIVMTQSPSTLSASVGDRVIINCQSSPSVYNNYLSWYQQKPGKAPKLLIYETSTLASGVPSRFSGSGSGAEFTLTISSLQPDDFATYYCAGTYVSGDRRAFGQGTKLTVL 114 AA PAT REGION 1..114 note BCMA VH source 1..114 mol_type protein organism synthetic construct QSVEESGGRLVTPGTPLTLTCTASGFSLSTYHMTWVRQAPGKGLEWIGVISSSGSTYYASWAKGRFTISKTSSTTVDLKMTSLTTEDTATYFCARDLDYVIDLWGQGTLVTVSS 110 AA PAT REGION 1..110 note BCMA VL source 1..110 mol_type protein organism synthetic construct AQVLTQTPSSVSSAVGGTVTINCQSSPSVYNNYLSWFQQKPGQPPKLLIYETSTLPSGVPSRFSASGSGTQFTLTISGVQCDDAATYYCAGTYVSGDRRAFGGGTEVVVK 122 AA PAT REGION 1..122 note CD19 VH source 1..122 mol_type protein organism synthetic construct QVQLQQSGAELVRPGSSVKISCKASGYAFSSYWMNWVKQRPGQGLEWIGQIYPGDGDTNYNGKFKGQATLTADKSSSTAYMQLSGLTSEDSAVYFCARKTISSVVDFYFDYWGQGTTLTVSS 108 AA PAT REGION 1..108 note CD19 VL source 1..108 mol_type protein organism synthetic construct DIVLTQSPKFMSTSVGDRVSVTCKASQNVGTNVAWYQQKPGQSPKPLIYSATYRNSGVPDRFTGSGSGTDFTLTITNVQSKDLADYFCQQYNRYPYTSGGGTKLEIKR 481 AA PAT REGION 1..481 note Loop CD19-BCMAscFv source 1..481 mol_type protein organism synthetic construct EIVMTQSPSTLSASVGDRVIINCQSSPSVYNNYLSWYQQKPGKAPKLLIYETSTLASGVPSRFSGSGSGAEFTLTISSLQPDDFATYYCAGTYVSGDRRAFGQGTKLTVLGGGGSDIVLTQSPKFMSTSVGDRVSVTCKASQNVGTNVAWYQQKPGQSPKPLIYSATYRNSGVPDRFTGSGSGTDFTLTITNVQSKDLADYFCQQYNRYPYTSGGGTKLEIKRGGGGSGGGGSGGGGSQVQLQQSGAELVRPGSSVKISCKASGYAFSSYWMNWVKQRPGQGLEWIGQIYPGDGDTNYNGKFKGQATLTADKSSSTAYMQLSGLTSEDSAVYFCARKTISSVVDFYFDYWGQGTTLTVSSGGGGSEVQLVESGGGLVQPGGSLRLSCTASGFSLSTYHMTWVRQAPGKGLEWIGVISSSGSTYYASWAKGRFTISRDNSKNTVYLQMNSLRAEDTAVYFCARDLDYVIDLWGPGTLVTVSS 481 AA PAT REGION 1..481 note Loop BCMA-CD19scFv source 1..481 mol_type protein organism synthetic construct DIVLTQSPKFMSTSVGDRVSVTCKASQNVGTNVAWYQQKPGQSPKPLIYSATYRNSGVPDRFTGSGSGTDFTLTITNVQSKDLADYFCQQYNRYPYTSGGGTKLEIKRGGGGSEIVMTQSPSTLSASVGDRVIINCQSSPSVYNNYLSWYQQKPGKAPKLLIYETSTLASGVPSRFSGSGSGAEFTLTISSLQPDDFATYYCAGTYVSGDRRAFGQGTKLTVLGGGGSGGGGSGGGGSEVQLVESGGGLVQPGGSLRLSCTASGFSLSTYHMTWVRQAPGKGLEWIGVISSSGSTYYASWAKGRFTISRDNSKNTVYLQMNSLRAEDTAVYFCARDLDYVIDLWGPGTLVTVSSGGGGSQVQLQQSGAELVRPGSSVKISCKASGYAFSSYWMNWVKQRPGQGLEWIGQIYPGDGDTNYNGKFKGQATLTADKSSSTAYMQLSGLTSEDSAVYFCARKTISSVVDFYFDYWGQGTTLTVSS 45 AA PAT source 1..45 mol_type protein organism synthetic construct TTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACD 24 AA PAT source 1..24 mol_type protein organism synthetic construct IYIWAPLAGTCGVLLLSLVITLYC 112 AA PAT source 1..112 mol_type protein organism synthetic construct RVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 42 AA PAT source 1..42 mol_type protein organism synthetic construct KRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCEL 21 AA PAT source 1..21 mol_type protein organism synthetic construct MALPVTALLLPLALLLHAARP 745 AA PAT REGION 1..745 note Tan CD19-BCMA source 1..745 mol_type protein organism synthetic construct MALPVTALLLPLALLLHAARPDIVLTQSPKFMSTSVGDRVSVTCKASQNVGTNVAWYQQKPGQSPKPLIYSATYRNSGVPDRFTGSGSGTDFTLTITNVQSKDLADYFCQQYNRYPYTSGGGTKLEIKRGGGGSGGGGSGGGGSQVQLQQSGAELVRPGSSVKISCKASGYAFSSYWMNWVKQRPGQGLEWIGQIYPGDGDTNYNGKFKGQATLTADKSSSTAYMQLSGLTSEDSAVYFCARKTISSVVDFYFDYWGQGTTLTVSSEAAAKEAAAKEAAAKEIVMTQSPSTLSASVGDRVIINCQSSPSVYNNYLSWYQQKPGKAPKLLIYETSTLASGVPSRFSGSGSGAEFTLTISSLQPDDFATYYCAGTYVSGDRRAFGQGTKLTVLGGGGSGGGGSGGGGSEVQLVESGGGLVQPGGSLRLSCTASGFSLSTYHMTWVRQAPGKGLEWIGVISSSGSTYYASWAKGRFTISRDNSKNTVYLQMNSLRAEDTAVYFCARDLDYVIDLWGPGTLVTVSSTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 745 AA PAT REGION 1..745 note Tan BCMA-CD19 source 1..745 mol_type protein organism synthetic construct MALPVTALLLPLALLLHAARPEIVMTQSPSTLSASVGDRVIINCQSSPSVYNNYLSWYQQKPGKAPKLLIYETSTLASGVPSRFSGSGSGAEFTLTISSLQPDDFATYYCAGTYVSGDRRAFGQGTKLTVLGGGGSGGGGSGGGGSEVQLVESGGGLVQPGGSLRLSCTASGFSLSTYHMTWVRQAPGKGLEWIGVISSSGSTYYASWAKGRFTISRDNSKNTVYLQMNSLRAEDTAVYFCARDLDYVIDLWGPGTLVTVSSEAAAKEAAAKEAAAKDIVLTQSPKFMSTSVGDRVSVTCKASQNVGTNVAWYQQKPGQSPKPLIYSATYRNSGVPDRFTGSGSGTDFTLTITNVQSKDLADYFCQQYNRYPYTSGGGTKLEIKRGGGGSGGGGSGGGGSQVQLQQSGAELVRPGSSVKISCKASGYAFSSYWMNWVKQRPGQGLEWIGQIYPGDGDTNYNGKFKGQATLTADKSSSTAYMQLSGLTSEDSAVYFCARKTISSVVDFYFDYWGQGTTLTVSSTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 725 AA PAT REGION 1..725 note Loop CD19-BCMA source 1..725 mol_type protein organism synthetic construct MALPVTALLLPLALLLHAARPEIVMTQSPSTLSASVGDRVIINCQSSPSVYNNYLSWYQQKPGKAPKLLIYETSTLASGVPSRFSGSGSGAEFTLTISSLQPDDFATYYCAGTYVSGDRRAFGQGTKLTVLGGGGSDIVLTQSPKFMSTSVGDRVSVTCKASQNVGTNVAWYQQKPGQSPKPLIYSATYRNSGVPDRFTGSGSGTDFTLTITNVQSKDLADYFCQQYNRYPYTSGGGTKLEIKRGGGGSGGGGSGGGGSQVQLQQSGAELVRPGSSVKISCKASGYAFSSYWMNWVKQRPGQGLEWIGQIYPGDGDTNYNGKFKGQATLTADKSSSTAYMQLSGLTSEDSAVYFCARKTISSVVDFYFDYWGQGTTLTVSSGGGGSEVQLVESGGGLVQPGGSLRLSCTASGFSLSTYHMTWVRQAPGKGLEWIGVISSSGSTYYASWAKGRFTISRDNSKNTVYLQMNSLRAEDTAVYFCARDLDYVIDLWGPGTLVTVSSTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 725 AA PAT REGION 1..725 note Loop BCMA-CD19 source 1..725 mol_type protein organism synthetic construct MALPVTALLLPLALLLHAARPDIVLTQSPKFMSTSVGDRVSVTCKASQNVGTNVAWYQQKPGQSPKPLIYSATYRNSGVPDRFTGSGSGTDFTLTITNVQSKDLADYFCQQYNRYPYTSGGGTKLEIKRGGGGSEIVMTQSPSTLSASVGDRVIINCQSSPSVYNNYLSWYQQKPGKAPKLLIYETSTLASGVPSRFSGSGSGAEFTLTISSLQPDDFATYYCAGTYVSGDRRAFGQGTKLTVLGGGGSGGGGSGGGGSEVQLVESGGGLVQPGGSLRLSCTASGFSLSTYHMTWVRQAPGKGLEWIGVISSSGSTYYASWAKGRFTISRDNSKNTVYLQMNSLRAEDTAVYFCARDLDYVIDLWGPGTLVTVSSGGGGSQVQLQQSGAELVRPGSSVKISCKASGYAFSSYWMNWVKQRPGQGLEWIGQIYPGDGDTNYNGKFKGQATLTADKSSSTAYMQLSGLTSEDSAVYFCARKTISSVVDFYFDYWGQGTTLTVSSTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 992 AA PAT REGION 1..992 note CD19-2A-BCMA source 1..992 mol_type protein organism synthetic construct MALPVTALLLPLALLLHAARPDIVLTQSPKFMSTSVGDRVSVTCKASQNVGTNVAWYQQKPGQSPKPLIYSATYRNSGVPDRFTGSGSGTDFTLTITNVQSKDLADYFCQQYNRYPYTSGGGTKLEIKRGGGGSGGGGSGGGGSQVQLQQSGAELVRPGSSVKISCKASGYAFSSYWMNWVKQRPGQGLEWIGQIYPGDGDTNYNGKFKGQATLTADKSSSTAYMQLSGLTSEDSAVYFCARKTISSVVDFYFDYWGQGTTLTVSSTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPREGRGSLLTCGDVEENPGPMALPVTALLLPLALLLHAARPEIVMTQSPSTLSASVGDRVIINCQSSPSVYNNYLSWYQQKPGKAPKLLIYETSTLASGVPSRFSGSGSGAEFTLTISSLQPDDFATYYCAGTYVSGDRRAFGQGTKLTVLGGGGSGGGGSGGGGSEVQLVESGGGLVQPGGSLRLSCTASGFSLSTYHMTWVRQAPGKGLEWIGVISSSGSTYYASWAKGRFTISRDNSKNTVYLQMNSLRAEDTAVYFCARDLDYVIDLWGPGTLVTVSSTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 992 AA PAT REGION 1..992 note BCMA-2A-CD19 source 1..992 mol_type protein organism synthetic construct MALPVTALLLPLALLLHAARPEIVMTQSPSTLSASVGDRVIINCQSSPSVYNNYLSWYQQKPGKAPKLLIYETSTLASGVPSRFSGSGSGAEFTLTISSLQPDDFATYYCAGTYVSGDRRAFGQGTKLTVLGGGGSGGGGSGGGGSEVQLVESGGGLVQPGGSLRLSCTASGFSLSTYHMTWVRQAPGKGLEWIGVISSSGSTYYASWAKGRFTISRDNSKNTVYLQMNSLRAEDTAVYFCARDLDYVIDLWGPGTLVTVSSTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPREGRGSLLTCGDVEENPGPMALPVTALLLPLALLLHAARPDIVLTQSPKFMSTSVGDRVSVTCKASQNVGTNVAWYQQKPGQSPKPLIYSATYRNSGVPDRFTGSGSGTDFTLTITNVQSKDLADYFCQQYNRYPYTSGGGTKLEIKRGGGGSGGGGSGGGGSQVQLQQSGAELVRPGSSVKISCKASGYAFSSYWMNWVKQRPGQGLEWIGQIYPGDGDTNYNGKFKGQATLTADKSSSTAYMQLSGLTSEDSAVYFCARKTISSVVDFYFDYWGQGTTLTVSSTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 489 AA PAT REGION 1..489 note CD19 CAR source 1..489 mol_type protein organism synthetic construct MALPVTALLLPLALLLHAARPDIVLTQSPKFMSTSVGDRVSVTCKASQNVGTNVAWYQQKPGQSPKPLIYSATYRNSGVPDRFTGSGSGTDFTLTITNVQSKDLADYFCQQYNRYPYTSGGGTKLEIKRGGGGSGGGGSGGGGSQVQLQQSGAELVRPGSSVKISCKASGYAFSSYWMNWVKQRPGQGLEWIGQIYPGDGDTNYNGKFKGQATLTADKSSSTAYMQLSGLTSEDSAVYFCARKTISSVVDFYFDYWGQGTTLTVSSTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 485 AA PAT REGION 1..485 note BCMA CAR source 1..485 mol_type protein organism synthetic construct MALPVTALLLPLALLLHAARPEIVMTQSPSTLSASVGDRVIINCQSSPSVYNNYLSWYQQKPGKAPKLLIYETSTLASGVPSRFSGSGSGAEFTLTISSLQPDDFATYYCAGTYVSGDRRAFGQGTKLTVLGGGGSGGGGSGGGGSEVQLVESGGGLVQPGGSLRLSCTASGFSLSTYHMTWVRQAPGKGLEWIGVISSSGSTYYASWAKGRFTISRDNSKNTVYLQMNSLRAEDTAVYFCARDLDYVIDLWGPGTLVTVSSTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 15 AA PAT source 1..15 mol_type protein organism synthetic construct GGGGSGGGGSGGGGS 5 AA PAT source 1..5 mol_type protein organism synthetic construct GGGGS 15 AA PAT source 1..15 mol_type protein organism synthetic construct EAAAKEAAAKEAAAK 348 DNA PAT misc_feature 1..348 note SEQ ID NO:13BCMA VH source 1..348 mol_type other DNA organism synthetic construct gaagtgcagctggtggagtccggcggtggactggtgcaaccgggaggctcactcagattgtcatgcaccgcctctggctttagtctctccacctatcatatgacttgggtgaggcaggcacccggcaagggcctggaatggatcggcgtgatctcttccagcggtagcacctattacgcctcttgggcgaagggcaggtttaccatcagccgcgacaacagcaagaataccgtttacctgcagatgaatagcctgagggccgaagacacggcggtctatttctgtgcacgggaccttgactacgttattgacctgtggggccctgggaccctcgtaactgtgagcagc 330 DNA PAT misc_feature 1..330 note SEQ ID NO:14BCMA VL source 1..330 mol_type other DNA organism synthetic construct gagatcgtgatgacccagtccccaagtacactgagcgcctccgtgggcgaccgcgtgatcataaactgtcaaagctcaccctctgtttacaacaattacctgtcttggtatcaacagaagcccggtaaggcccccaaactgctcatttacgagacatccaccctggcatccggggtgccaagccgcttctccgggagtgggtctggcgccgagttcaccctgaccatatcttccctgcagcccgacgacttcgcaacgtactattgcgccggaacctatgtaagtggggatagacgcgccttcgggcagggcacgaagttgaccgtgctg 366 DNA PAT misc_feature 1..366 note SEQ ID NO:17CD19 VH source 1..366 mol_type other DNA organism synthetic construct caagtgcaactgcaacagagcggagccgaactggtgagacccggaagcagcgtgaagatcagctgcaaggcttccggctacgcctttagcagctactggatgaactgggtgaagcagagacctggacagggactggaatggatcggccagatttaccctggagacggcgacacaaactacaacggcaagttcaagggccaagctacactgaccgccgacaaaagcagcagcaccgcctatatgcagctgagcggactgaccagcgaagatagcgctgtgtacttctgcgccagaaagaccatcagcagcgtggtggacttctacttcgactactggggacaaggcaccaccctgacagtgagcagc 324 DNA PAT misc_feature 1..324 note SEQ ID NO:18CD19 VL source 1..324 mol_type other DNA organism synthetic construct gatattgtgctgacccagagccccaagttcatgagcaccagcgtgggcgatagagtgagcgtgacctgcaaggcaagccagaacgtgggaacaaacgtggcctggtaccaacagaaacccggccaaagccctaagcccctgatttacagcgccacctacagaaatagcggcgtgcccgacagatttacaggaagcggcagcggaaccgatttcacactgaccatcaccaacgtgcagagcaaagacctggccgactacttctgccagcagtacaacagatacccctacaccagcggaggaggaacaaagctggagatcaagaga