0342-PA-034 CN CN2023112870743 2023-09-28 上海天泽云泰生物医药有限公司 SHANGHAI VITALGEN BIOPHARMA CO., LTD. 瞿苏 QU Su 用于治疗血红蛋白疾病的组合物和方法 92 1153 AA PAT REGION 1..1153 note Cas12b-M1 SITE 1153 note misc_feature - Xaa = source 1..1153 mol_type protein organism synthetic construct MHHHHHHPKKKRKVPGAVKSMKVKLRLDNMPEIRAGLWKLHTEVNAGVRYYTEWLSLLRQENLYRRSPNGDGEQECYKTAEECKAELLERLRARQVENGHCGPAGSDDELLQLARQLYELLVPQAIGAKGDAQFIARKFLSPLADKDAVGGLGIAKAGNKPRWVRMREAGEPGWEEEKAKAEARKSTDRTADVLRALADFGLKPLMRVYTDSDMSSVQWKPLRKGQAVRTWDRDMFQQAIERMMSWESWNQRVGEAYAKLVEQKSRFEQKNFVGQEHLVQLVNQLQQDMKEASHGLESKEQTAHYLTGRALRGSDKVFEKWEKLDPDAPFDLYDTEIKNVQRRNTRRFGSHDLFAKLAEPKYQALWREDASFLTRYAVYNSIVRKLNHAKMFATFTLPDATAHPIWTRFDKLGGNLHQYTFLFNEFGEGRHAIRFQKLLTVEDGVAKEVDDVTVPISMSAQLDDLLPRDPHELVALYFQDYGAEQHLAGRFGGAKIQYRRDQLNHLHARRGARDVYLNLSVRVQSQSEARGERRPPYAAVFRLVGDNHRAFVHFDKLSDYLAEHPDDGKLGSEGLLSGLRVMSVDLGLRTSASISVFRVARKDELKPNSEGRVPFCFPIEGNENLVAVHERSQLLKLPGETESKDLRAIREERQRTLRQLRTQLAYLRLLVRCGSEDVGRRERSWAKLIEQPMDANQMTPDWREAFEDELQKLKSLYGICGDREWTEAVYESVRRVWRHGKQVRDWRKDVRSGERPKIRGYQKDVVGGNSIRQIEYLERQYKFLKSWSFFGKVSGQVIRAEKGSRFAITLREHIDHAKEDRLKKLADRIIMEALGYVYALDDERGKGKWVAKYPPCQLILLEELSEYQFNNDRPPSENNQLMQWSHRGVFQELLNQAQVHDLLVGTMYAAFSSRFDARTGAPGIRCRRVPARCAREQNPEPFPWWLNKFVAEHKLDGCPLRADDLIPTGEGEFFVSPFSAEEGDFHQIHADLNAAQNLQRRLWSDFDISQIRLRCDWGEVDGEPVLIPRTTGKRTADSYGNKVFYTKTGVTYYERERGKKRRKVFAQEELSEEEAELLVEADEAREKSVVLMRDPSGIINRGDWTRQKEFWSMVNQRIEGYLVKQIRSRVRLQESACENTGDIASPKKKRKVX 1147 AA PAT REGION 1..1147 note Cas12b-M2 SITE 1147 note misc_feature - Xaa = source 1..1147 mol_type protein organism synthetic construct MPKKKRKVPGAVKSMKVKLRLDNMPEIRAGLWKLHTEVNAGVRYYTEWLSLLRQENLYRRSPNGDGEQECYKTAEECKAELLERLRARQVENGHCGPAGSDDELLQLARQLYELLVPQAIGAKGDAQFIARKFLSPLADKDAVGGLGIAKAGNKPRWVRMREAGEPGWEEEKAKAEARKSTDRTADVLRALADFGLKPLMRVYTDSDMSSVQWKPLRKGQAVRTWDRDMFQQAIERMMSWESWNQRVGEAYAKLVEQKSRFEQKNFVGQEHLVQLVNQLQQDMKEASHGLESKEQTAHYLTGRALRGSDKVFEKWEKLDPDAPFDLYDTEIKNVQRRNTRRFGSHDLFAKLAEPKYQALWREDASFLTRYAVYNSIVRKLNHAKMFATFTLPDATAHPIWTRFDKLGGNLHQYTFLFNEFGEGRHAIRFQKLLTVEDGVAKEVDDVTVPISMSAQLDDLLPRDPHELVALYFQDYGAEQHLAGRFGGAKIQYRRDQLNHLHARRGARDVYLNLSVRVQSQSEARGERRPPYAAVFRLVGDNHRAFVHFDKLSDYLAEHPDDGKLGSEGLLSGLRVMSVDLGLRTSASISVFRVARKDELKPNSEGRVPFCFPIEGNENLVAVHERSQLLKLPGETESKDLRAIREERQRTLRQLRTQLAYLRLLVRCGSEDVGRRERSWAKLIEQPMDANQMTPDWREAFEDELQKLKSLYGICGDREWTEAVYESVRRVWRHGKQVRDWRKDVRSGERPKIRGYQKDVVGGNSIRQIEYLERQYKFLKSWSFFGKVSGQVIRAEKGSRFAITLREHIDHAKEDRLKKLADRIIMEALGYVYALDDERGKGKWVAKYPPCQLILLEELSEYQFNNDRPPSENNQLMQWSHRGVFQELLNQAQVHDLLVGTMYAAFSSRFDARTGAPGIRCRRVPARCAREQNPEPFPWWLNKFVAEHKLDGCPLRADDLIPTGEGEFFVSPFSAEEGDFHQIHADLNAAQNLQRRLWSDFDISQIRLRCDWGEVDGEPVLIPRTTGKRTADSYGNKVFYTKTGVTYYERERGKKRRKVFAQEELSEEEAELLVEADEAREKSVVLMRDPSGIINRGDWTRQKEFWSMVNQRIEGYLVKQIRSRVRLQESACENTGDIASPKKKRKVX 1167 AA PAT REGION 1..1167 note Cas12b-M3 SITE 1167 note misc_feature - Xaa = source 1..1167 mol_type protein organism synthetic construct MPKKKRKVPGAVKSMKVKLRLDNMPEIRAGLWKLHTEVNAGVRYYTEWLSLLRQENLYRRSPNGDGEQECYKTAEECKAELLERLRARQVENGHCGPAGSDDELLQLARQLYELLVPQAIGAKGDAQFIARKFLSPLADKDAVGGLGIAKAGNKPRWVRMREAGEPGWEEEKAKAEARKSTDRTADVLRALADFGLKPLMRVYTDSDMSSVQWKPLRKGQAVRTWDRDMFQQAIERMMSWESWNQRVGEAYAKLVEQKSRFEQKNFVGQEHLVQLVNQLQQDMKEASHGLESKEQTAHYLTGRALRGSDKVFEKWEKLDPDAPFDLYDTEIKNVQRRNTRRFGSHDLFAKLAEPKYQALWREDASFLTRYAVYNSIVRKLNHAKMFATFTLPDATAHPIWTRFDKLGGNLHQYTFLFNEFGEGRHAIRFQKLLTVEDGVAKEVDDVTVPISMSAQLDDLLPRDPHELVALYFQDYGAEQHLAGRFGGAKIQYRRDQLNHLHARRGARDVYLNLSVRVQSQSEARGERRPPYAAVFRLVGDNHRAFVHFDKLSDYLAEHPDDGKLGSEGLLSGLRVMSVDLGLRTSASISVFRVARKDELKPNSEGRVPFCFPIEGNENLVAVHERSQLLKLPGETESKDLRAIREERQRTLRQLRTQLAYLRLLVRCGSEDVGRRERSWAKLIEQPMDANQMTPDWREAFEDELQKLKSLYGICGDREWTEAVYESVRRVWRHGKQVRDWRKDVRSGERPKIRGYQKDVVGGNSIRQIEYLERQYKFLKSWSFFGKVSGQVIRAEKGSRFAITLREHIDHAKEDRLKKLADRIIMEALGYVYALDDERGKGKWVAKYPPCQLILLEELSEYQFNNDRPPSENNQLMQWSHRGVFQELLNQAQVHDLLVGTMYAAFSSRFDARTGAPGIRCRRVPARCAREQNPEPFPWWLNKFVAEHKLDGCPLRADDLIPTGEGEFFVSPFSAEEGDFHQIHADLNAAQNLQRRLWSDFDISQIRLRCDWGEVDGEPVLIPRTTGKRTADSYGNKVFYTKTGVTYYERERGKKRRKVFAQEELSEEEAELLVEADEAREKSVVLMRDPSGIINRGDWTRQKEFWSMVNQRIEGYLVKQIRSRVRLQESACENTGDIASPKKKRKVGSGSKRPAATKKAGQAKKKKX 1160 AA PAT REGION 1..1160 note Cas12b-M4 SITE 1160 note misc_feature - Xaa = source 1..1160 mol_type protein organism synthetic construct MPKKKRKVPGAVKSMKVKLRLDNMPEIRAGLWKLHTEVNAGVRYYTEWLSLLRQENLYRRSPNGDGEQECYKTAEECKAELLERLRARQVENGHCGPAGSDDELLQLARQLYELLVPQAIGAKGDAQFIARKFLSPLADKDAVGGLGIAKAGNKPRWVRMREAGEPGWEEEKAKAEARKSTDRTADVLRALADFGLKPLMRVYTDSDMSSVQWKPLRKGQAVRTWDRDMFQQAIERMMSWESWNQRVGEAYAKLVEQKSRFEQKNFVGQEHLVQLVNQLQQDMKEASHGLESKEQTAHYLTGRALRGSDKVFEKWEKLDPDAPFDLYDTEIKNVQRRNTRRFGSHDLFAKLAEPKYQALWREDASFLTRYAVYNSIVRKLNHAKMFATFTLPDATAHPIWTRFDKLGGNLHQYTFLFNEFGEGRHAIRFQKLLTVEDGVAKEVDDVTVPISMSAQLDDLLPRDPHELVALYFQDYGAEQHLAGRFGGAKIQYRRDQLNHLHARRGARDVYLNLSVRVQSQSEARGERRPPYAAVFRLVGDNHRAFVHFDKLSDYLAEHPDDGKLGSEGLLSGLRVMSVDLGLRTSASISVFRVARKDELKPNSEGRVPFCFPIEGNENLVAVHERSQLLKLPGETESKDLRAIREERQRTLRQLRTQLAYLRLLVRCGSEDVGRRERSWAKLIEQPMDANQMTPDWREAFEDELQKLKSLYGICGDREWTEAVYESVRRVWRHGKQVRDWRKDVRSGERPKIRGYQKDVVGGNSIRQIEYLERQYKFLKSWSFFGKVSGQVIRAEKGSRFAITLREHIDHAKEDRLKKLADRIIMEALGYVYALDDERGKGKWVAKYPPCQLILLEELSEYQFNNDRPPSENNQLMQWSHRGVFQELLNQAQVHDLLVGTMYAAFSSRFDARTGAPGIRCRRVPARCAREQNPEPFPWWLNKFVAEHKLDGCPLRADDLIPTGEGEFFVSPFSAEEGDFHQIHADLNAAQNLQRRLWSDFDISQIRLRCDWGEVDGEPVLIPRTTGKRTADSYGNKVFYTKTGVTYYERERGKKRRKVFAQEELSEEEAELLVEADEAREKSVVLMRDPSGIINRGDWTRQKEFWSMVNQRIEGYLVKQIRSRVRLQESACENTGDIASPKKKRKVGSGSPAAKKKKLDX 1180 AA PAT REGION 1..1180 note Cas12b-M5 SITE 1180 note misc_feature - Xaa = source 1..1180 mol_type protein organism synthetic construct MPAAKKKKLDGSGSPKKKRKVPGAVKSMKVKLRLDNMPEIRAGLWKLHTEVNAGVRYYTEWLSLLRQENLYRRSPNGDGEQECYKTAEECKAELLERLRARQVENGHCGPAGSDDELLQLARQLYELLVPQAIGAKGDAQFIARKFLSPLADKDAVGGLGIAKAGNKPRWVRMREAGEPGWEEEKAKAEARKSTDRTADVLRALADFGLKPLMRVYTDSDMSSVQWKPLRKGQAVRTWDRDMFQQAIERMMSWESWNQRVGEAYAKLVEQKSRFEQKNFVGQEHLVQLVNQLQQDMKEASHGLESKEQTAHYLTGRALRGSDKVFEKWEKLDPDAPFDLYDTEIKNVQRRNTRRFGSHDLFAKLAEPKYQALWREDASFLTRYAVYNSIVRKLNHAKMFATFTLPDATAHPIWTRFDKLGGNLHQYTFLFNEFGEGRHAIRFQKLLTVEDGVAKEVDDVTVPISMSAQLDDLLPRDPHELVALYFQDYGAEQHLAGRFGGAKIQYRRDQLNHLHARRGARDVYLNLSVRVQSQSEARGERRPPYAAVFRLVGDNHRAFVHFDKLSDYLAEHPDDGKLGSEGLLSGLRVMSVDLGLRTSASISVFRVARKDELKPNSEGRVPFCFPIEGNENLVAVHERSQLLKLPGETESKDLRAIREERQRTLRQLRTQLAYLRLLVRCGSEDVGRRERSWAKLIEQPMDANQMTPDWREAFEDELQKLKSLYGICGDREWTEAVYESVRRVWRHGKQVRDWRKDVRSGERPKIRGYQKDVVGGNSIRQIEYLERQYKFLKSWSFFGKVSGQVIRAEKGSRFAITLREHIDHAKEDRLKKLADRIIMEALGYVYALDDERGKGKWVAKYPPCQLILLEELSEYQFNNDRPPSENNQLMQWSHRGVFQELLNQAQVHDLLVGTMYAAFSSRFDARTGAPGIRCRRVPARCAREQNPEPFPWWLNKFVAEHKLDGCPLRADDLIPTGEGEFFVSPFSAEEGDFHQIHADLNAAQNLQRRLWSDFDISQIRLRCDWGEVDGEPVLIPRTTGKRTADSYGNKVFYTKTGVTYYERERGKKRRKVFAQEELSEEEAELLVEADEAREKSVVLMRDPSGIINRGDWTRQKEFWSMVNQRIEGYLVKQIRSRVRLQESACENTGDIASPKKKRKVGSGSKRPAATKKAGQAKKKKX 1173 AA PAT REGION 1..1173 note Cas12b-M6 SITE 1173 note misc_feature - Xaa = source 1..1173 mol_type protein organism synthetic construct MPGAVKSMKVKLRLDNMPEIRAGLWKLHTEVNAGVRYYTEWLSLLRQENLYRRSPNGDGEQECYKTAEECKAELLERLRARQVENGHCGPAGSDDELLQLARQLYELLVPQAIGAKGDAQFIARKFLSPLADKDAVGGLGIAKAGNKPRWVRMREAGEPGWEEEKAKAEARKSTDRTADVLRALADFGLKPLMRVYTDSDMSSVQWKPLRKGQAVRTWDRDMFQQAIERMMSWESWNQRVGEAYAKLVEQKSRFEQKNFVGQEHLVQLVNQLQQDMKEASHGLESKEQTAHYLTGRALRGSDKVFEKWEKLDPDAPFDLYDTEIKNVQRRNTRRFGSHDLFAKLAEPKYQALWREDASFLTRYAVYNSIVRKLNHAKMFATFTLPDATAHPIWTRFDKLGGNLHQYTFLFNEFGEGRHAIRFQKLLTVEDGVAKEVDDVTVPISMSAQLDDLLPRDPHELVALYFQDYGAEQHLAGRFGGAKIQYRRDQLNHLHARRGARDVYLNLSVRVQSQSEARGERRPPYAAVFRLVGDNHRAFVHFDKLSDYLAEHPDDGKLGSEGLLSGLRVMSVDLGLRTSASISVFRVARKDELKPNSEGRVPFCFPIEGNENLVAVHERSQLLKLPGETESKDLRAIREERQRTLRQLRTQLAYLRLLVRCGSEDVGRRERSWAKLIEQPMDANQMTPDWREAFEDELQKLKSLYGICGDREWTEAVYESVRRVWRHGKQVRDWRKDVRSGERPKIRGYQKDVVGGNSIRQIEYLERQYKFLKSWSFFGKVSGQVIRAEKGSRFAITLREHIDHAKEDRLKKLADRIIMEALGYVYALDDERGKGKWVAKYPPCQLILLEELSEYQFNNDRPPSENNQLMQWSHRGVFQELLNQAQVHDLLVGTMYAAFSSRFDARTGAPGIRCRRVPARCAREQNPEPFPWWLNKFVAEHKLDGCPLRADDLIPTGEGEFFVSPFSAEEGDFHQIHADLNAAQNLQRRLWSDFDISQIRLRCDWGEVDGEPVLIPRTTGKRTADSYGNKVFYTKTGVTYYERERGKKRRKVFAQEELSEEEAELLVEADEAREKSVVLMRDPSGIINRGDWTRQKEFWSMVNQRIEGYLVKQIRSRVRLQESACENTGDIASPKKKRKVGSGSPAAKKKKLDGSGSKRPAATKKAGQAKKKKX 1171 AA PAT REGION 1..1171 note Cas12b-M7 SITE 1171 note misc_feature - Xaa = source 1..1171 mol_type protein organism synthetic construct MKRTADGSEFESPKKKRKVEPGAVKSMKVKLRLDNMPEIRAGLWKLHTEVNAGVRYYTEWLSLLRQENLYRRSPNGDGEQECYKTAEECKAELLERLRARQVENGHCGPAGSDDELLQLARQLYELLVPQAIGAKGDAQFIARKFLSPLADKDAVGGLGIAKAGNKPRWVRMREAGEPGWEEEKAKAEARKSTDRTADVLRALADFGLKPLMRVYTDSDMSSVQWKPLRKGQAVRTWDRDMFQQAIERMMSWESWNQRVGEAYAKLVEQKSRFEQKNFVGQEHLVQLVNQLQQDMKEASHGLESKEQTAHYLTGRALRGSDKVFEKWEKLDPDAPFDLYDTEIKNVQRRNTRRFGSHDLFAKLAEPKYQALWREDASFLTRYAVYNSIVRKLNHAKMFATFTLPDATAHPIWTRFDKLGGNLHQYTFLFNEFGEGRHAIRFQKLLTVEDGVAKEVDDVTVPISMSAQLDDLLPRDPHELVALYFQDYGAEQHLAGRFGGAKIQYRRDQLNHLHARRGARDVYLNLSVRVQSQSEARGERRPPYAAVFRLVGDNHRAFVHFDKLSDYLAEHPDDGKLGSEGLLSGLRVMSVDLGLRTSASISVFRVARKDELKPNSEGRVPFCFPIEGNENLVAVHERSQLLKLPGETESKDLRAIREERQRTLRQLRTQLAYLRLLVRCGSEDVGRRERSWAKLIEQPMDANQMTPDWREAFEDELQKLKSLYGICGDREWTEAVYESVRRVWRHGKQVRDWRKDVRSGERPKIRGYQKDVVGGNSIRQIEYLERQYKFLKSWSFFGKVSGQVIRAEKGSRFAITLREHIDHAKEDRLKKLADRIIMEALGYVYALDDERGKGKWVAKYPPCQLILLEELSEYQFNNDRPPSENNQLMQWSHRGVFQELLNQAQVHDLLVGTMYAAFSSRFDARTGAPGIRCRRVPARCAREQNPEPFPWWLNKFVAEHKLDGCPLRADDLIPTGEGEFFVSPFSAEEGDFHQIHADLNAAQNLQRRLWSDFDISQIRLRCDWGEVDGEPVLIPRTTGKRTADSYGNKVFYTKTGVTYYERERGKKRRKVFAQEELSEEEAELLVEADEAREKSVVLMRDPSGIINRGDWTRQKEFWSMVNQRIEGYLVKQIRSRVRLQESACENTGDIASKRTADGSEFESPKKKRKVEX 1182 AA PAT REGION 1..1182 note Cas12b-M8 SITE 1182 note misc_feature - Xaa = source 1..1182 mol_type protein organism synthetic construct MPKKKRKVTRMAVKSMKVKLRLDNMPEIRAGLWKLHTEVNAGVRYYTEWLSLLRQENLYRRSPNGDGEQECYKTAEECKAELLERLRARQVENGHCGPAGSDDELLQLARQLYELLVPQAIGAKGDAQFIARKFLSPLADKDAVGGLGIAKAGNKPRWVRMREAGEPGWEEEKAKAEARKSTDRTADVLRALADFGLKPLMRVYTDSDMSSVQWKPLRKGQAVRTWDRDMFQQAIERMMSWESWNQRVGEAYAKLVEQKSRFEQKNFVGQEHLVQLVNQLQQDMKEASHGLESKEQTAHYLTGRALRGSDKVFEKWEKLDPDAPFDLYDTEIKNVQRRNTRRFGSHDLFAKLAEPKYQALWREDASFLTRYAVYNSIVRKLNHAKMFATFTLPDATAHPIWTRFDKLGGNLHQYTFLFNEFGEGRHAIRFQKLLTVEDGVAKEVDDVTVPISMSAQLDDLLPRDPHELVALYFQDYGAEQHLAGRFGGAKIQYRRDQLNHLHARRGARDVYLNLSVRVQSQSEARGERRPPYAAVFRLVGDNHRAFVHFDKLSDYLAEHPDDGKLGSEGLLSGLRVMSVDLGLRTSASISVFRVARKDELKPNSEGRVPFCFPIEGNENLVAVHERSQLLKLPGETESKDLRAIREERQRTLRQLRTQLAYLRLLVRCGSEDVGRRERSWAKLIEQPMDANQMTPDWREAFEDELQKLKSLYGICGDREWTEAVYESVRRVWRHMGKQVRDWRKDVRSGERPKIRGYQKDVVGGNSIRQIEYLERQYKFLKSWSFFGKVSGQVIRAEKGSRFAITLREHIDHAKEDRLKKLADRIIMEALGYVYALDDERGKGKWVAKYPPCQLILLEELSEYQFNNDRPPSENNQLMQWSHRGVFQELLNQAQVHDLLVGTMYAAFSSRFDARTGAPGIRCRRVPARCAREQNPEPFPWWLNKFVAEHKLDGCPLRADDLIPTGEGEFFVSPFSAEEGDFHQIHADLNAAQNLQRRLWSDFDISQIRLRCDWGEVDGEPVLIPRTTGKRTADSYGNKVFYTKTGVTYYERERGKKRRKVFAQEELSEEEAELLVEADEAREKSVVLMRDPSGIINRGDWTRQKEFWSMVNQRIEGYLVKQIRSRVRLQESACENTGDIASPKKKRKVGSGSPAAKKKKLDGSGSKRPAATKKAGQAKKKKX 1080 AA PAT REGION 1..1080 note Cas12i-M1 SITE 1080 note misc_feature - Xaa = source 1..1080 mol_type protein organism synthetic construct MHHHHHHPKKKRKVPGMSSAIKSYKSVLRPNERKNQLLKSTIQCLEDGSAFFFKMLQGLFGGITPEIVRFSTEQEKQQQDIALWCAVNWFRPVSQDSLTHTIASDNLVEKFEEYYGGTASDAIKQYFSASIGESYYWNDCRQQYYDLCRELGVEVSDLTHDLEILCREKCLAVATESNQYNSIISVLFGTGRKEDRSVKLRITKKILEAISNLKEIPKNVAPIQEIILNVAKATKETFRQVYAGNLGAPSTLERFIAKDGQKEFDLKKLQTDLKKVIRGKSKERDWCCQEELRSYVEQNTIQYDLWAWGEMFNKAHTALKIKSTRNYNFAKQRLEQFKRIQSLNNLLVVKKLNDFFDSEFFSGEETYTICVHHLGGKRLSKLYKAWEDDPADPENAIVVLCDDLKNNFKAEPIRNILRYIFTIRQECSAQDILAAAKYNQQLDRYKSQKANPSVLGNQGFTWRNAVILPEKAQRNDRPNSLDLRIWLYLKLRHPDGRWKKHHIPFYDTRFFQEIYAAGNSPVDTCQFRTPRFGYHLPKLTDQTAIRVNKKHVKAAKTEARIRLAIQQGTLPVSNLKITRISATINSKGQVRIPVKFDVGRQKGTLQIGDRFCGYDQNQTASHAYSLWEVVKEGQYHKELGCFVRFISSGDIVSITENRGNQFDQLSYEGLAYPQYADWRKKASKFVSLWQITKKNKKKEIVTVEAKEKFDAICKYQPRLYKFNKEYAYLLRDIVRGKSLVELQQIRQEIFRFIEQDCGVTRLGSLSLSTLETVKAVKGIIYSYFSTALNASKNNPISDEQRKEFDPELFALLEKLELIRTRKKKQKVERIANSLIQTCLENNIKFIRGEGDLSTTNNATKKKANSRSMDWLARGVFNKIRQLAPMHNITLFGCGSLYTSHQDPLVHRNPDKAMKCRWAAIPVKDIGDWVLRKLSQNLRAKNIGTGEYYHQGVKEFLSHYELQDLEEELLKWRSDRKSNIPCWVLQNRLAEKLGNKEAVVYIPVRGGRIYFATHKVATGAVSIVFDQKQVWVCNADHVAAANIALTVKGIGEQSSDEENPDGSRIKLQLTSASPKKKRKVX 1080 AA PAT REGION 1..1080 note Cas12i-M2 SITE 1080 note misc_feature - Xaa = source 1..1080 mol_type protein organism synthetic construct MHHHHHHPKKKRKVPGMSSAIKSYKSVLRPNERKNQLLKSTIQCLEDGSAFFFKMLQGLFGGITPEIVRFSTEQEKQQQDIALWCAVNWFRPVSQDSLTHTIASDNLVEKFEEYYGGTASDAIKQYFSASIGESYYWNDCRQQYYDLCRELGVEVSDLTHDLEILCREKCLAVATESNQYNSIISVLFGTGRKEDRSVKLRITKKILEAISNLKEIPKNVAPIQEIILNVAKATKETFRQVYAGNLGAPSTLERFIAKDGQKEFDLKKLQTDLKKVIRGKSKERDWCCQEELRSYVEQNTIQYDLWAWGEMFNKAHTALKIKSTRNYNFAKQRLEQFKRIQSLNNLLVVKKLNDFFDSEFFSGEETYTICVHHLGGKRLSKLYKAWEDDPADPENAIVVLCDDLKNNFKKEPIRNILRYIFTIRQECSAQDILAAAKYNQQLDRYKSQKANPSVLGNQGFTWRNAVILPEKAQRNDRPNSLDLRIWLYLKLRHPDGRWKKHHIPFYDTRFFQEIYAAGNSPVDTCQFRTPRFGYHLPKLTDQTAIRVNKKHVKAAKTEARIRLAIQQGTLPVSNLKITRISATINSKGQVRIPVKFDVGRQKGTLQIGDRFCGYDQNQTASHAYSLWEVVKEGQYHKELGCFVRFISSGDIVSITENRGNQFDQLSYEGLAYPQYADWRKKASKFVSLWQITKKNKKKEIVTVEAKEKFDAICKYQPRLYKFNKEYAYLLRDIVRGKSLVELQQIRQEIFRFIEQDCGVTRLGSLSLSTLETVKAVKGIIYSYFSTALNASKNNPISDEQRKEFDPELFALLEKLELIRTRKKKQKVERIANSLIQTCLENNIKFIRGEGDLSTTNNATKKKANSRSMDWLARGVFNKIRQLAPMHNITLFGCGSLYTSHQDPLVHRNPDKAMKCRWAAIPVKDIGDWVLRKLSQNLRAKNIGTGEYYHQGVKEFLSHYELQDLEEELLKWRSDRKSNIPCWVLQNRLAEKLGNKEAVVYIPVRGGRIYFATHKVATGAVSIVFDQKQVWVCNADHVAAANIALTVKGIGEQSSDEENPDGSRIKLQLTSASPKKKRKVX 1072 AA PAT REGION 1..1072 note Cas12i-M3 source 1..1072 mol_type protein organism synthetic construct PKKKRKVPGMSSAIKSYKSVLRPNERKNQLLKSTIQCLEDGSAFFFKMLQGLFGGITPEIVRFSTEQEKQQQDIALWCAVNWFRPVSQDSLTHTIASDNLVEKFEEYYGGTASDAIKQYFSASIGESYYWNDCRQQYYDLCRELGVEVSDLTHDLEILCREKCLAVATESNQYNSIISVLFGTGRKEDRSVKLRITKKILEAISNLKEIPKNVAPIQEIILNVAKATKETFRQVYAGNLGAPSTLERFIAKDGQKEFDLKKLQTDLKKVIRGKSKERDWCCQEELRSYVEQNTIQYDLWAWGEMFNKAHTALKIKSTRNYNFAKQRLEQFKRIQSLNNLLVVKKLNDFFDSEFFSGEETYTICVHHLGGKRLSKLYKAWEDDPADPENAIVVLCDDLKNNFKKEPIRNILRYIFTIRQECSAQDILAAAKYNQQLDRYKSQKANPSVLGNQGFTWRNAVILPEKAQRNDRPNSLDLRIWLYLKLRHPDGRWKKHHIPFYDTRFFQEIYAAGNSPVDTCQFRTPRFGYHLPKLTDQTAIRVNKKHVKAAKTEARIRLAIQQGTLPVSNLKITRISATINSKGQVRIPVKFDVGRQKGTLQIGDRFCGYDQNQTASHAYSLWEVVKEGQYHKELGCFVRFISSGDIVSITENRGNQFDQLSYEGLAYPQYADWRKKASKFVSLWQITKKNKKKEIVTVEAKEKFDAICKYQPRLYKFNKEYAYLLRDIVRGKSLVELQQIRQEIFRFIEQDCGVTRLGSLSLSTLETVKAVKGIIYSYFSTALNASKNNPISDEQRKEFDPELFALLEKLELIRTRKKKQKVERIANSLIQTCLENNIKFIRGEGDLSTTNNATKKKANSRSMDWLARGVFNKIRQLAPMHNITLFGCGSLYTSHQDPLVHRNPDKAMKCRWAAIPVKDIGDWVLRKLSQNLRAKNIGTGEYYHQGVKEFLSHYELQDLEEELLKWRSDRKSNIPCWVLQNRLAEKLGNKEAVVYIPVRGGRIYFATHKVATGAVSIVFDQKQVWVCNADHVAAANIALTVKGIGEQSSDEENPDGSRIKLQLTSASPKKKRKV 111 DNA PAT misc_feature 1..111 note 12b-gRNA1-18 source 1..111 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 109..111 mod_base OTHER note 2'-O-methyl nucleotide modified_base 108..111 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacagatagtgtggggaaggg 113 DNA PAT misc_feature 1..113 note 12b-gRNA1-20 source 1..113 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 111..113 mod_base OTHER note 2'-O-methyl nucleotide modified_base 110..113 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacagatagtgtggggaaggggc 114 DNA PAT misc_feature 1..114 note 12b-gRNA1-21 source 1..114 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 112..114 mod_base OTHER note 2'-O-methyl nucleotide modified_base 111..114 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacagatagtgtggggaaggggcc 115 DNA PAT misc_feature 1..115 note 12b-gRNA1-22 source 1..115 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 113..115 mod_base OTHER note 2'-O-methyl nucleotide modified_base 112..115 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacagatagtgtggggaaggggccc 116 DNA PAT misc_feature 1..116 note 12b-gRNA1-23 source 1..116 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 114..116 mod_base OTHER note 2'-O-methyl nucleotide modified_base 113..116 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacagatagtgtggggaaggggcccc 117 DNA PAT misc_feature 1..117 note 12b-gRNA1-24 source 1..117 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 115..117 mod_base OTHER note 2'-O-methyl nucleotide modified_base 114..117 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacagatagtgtggggaaggggccccc 118 DNA PAT misc_feature 1..118 note 12b-gRNA1-25 source 1..118 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 116..118 mod_base OTHER note 2'-O-methyl nucleotide modified_base 115..118 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacagatagtgtggggaaggggccccca 119 DNA PAT misc_feature 1..119 note 12b-gRNA1-26 source 1..119 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 117..119 mod_base OTHER note 2'-O-methyl nucleotide modified_base 116..119 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacagatagtgtggggaaggggcccccaa 120 DNA PAT misc_feature 1..120 note 12b-gRNA1-27 source 1..120 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 118..120 mod_base OTHER note 2'-O-methyl nucleotide modified_base 117..120 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacagatagtgtggggaaggggcccccaag 121 DNA PAT misc_feature 1..121 note 12b-gRNA1-28 source 1..121 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 119..121 mod_base OTHER note 2'-O-methyl nucleotide modified_base 118..121 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacagatagtgtggggaaggggcccccaaga 122 DNA PAT misc_feature 1..122 note 12b-gRNA1-29 source 1..122 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 120..122 mod_base OTHER note 2'-O-methyl nucleotide modified_base 119..122 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacagatagtgtggggaaggggcccccaagag 123 DNA PAT misc_feature 1..123 note 12b-gRNA1-30 source 1..123 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 121..123 mod_base OTHER note 2'-O-methyl nucleotide modified_base 120..123 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacagatagtgtggggaaggggcccccaagagg 124 DNA PAT misc_feature 1..124 note 12b-gRNA1-31 source 1..124 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 122..124 mod_base OTHER note 2'-O-methyl nucleotide modified_base 121..124 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacagatagtgtggggaaggggcccccaagagga 111 DNA PAT misc_feature 1..111 note 12b-gRNA2-18 source 1..111 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 109..111 mod_base OTHER note 2'-O-methyl nucleotide modified_base 108..111 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcaccattgagatagtgtgggg 113 DNA PAT misc_feature 1..113 note 12b-gRNA2-20 source 1..113 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 111..113 mod_base OTHER note 2'-O-methyl nucleotide modified_base 110..113 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcaccattgagatagtgtggggaa 114 DNA PAT misc_feature 1..114 note 12b-gRNA2-21 source 1..114 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 112..114 mod_base OTHER note 2'-O-methyl nucleotide modified_base 111..114 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcaccattgagatagtgtggggaag 115 DNA PAT misc_feature 1..115 note 12b-gRNA2-22 source 1..115 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 113..115 mod_base OTHER note 2'-O-methyl nucleotide modified_base 112..115 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcaccattgagatagtgtggggaagg 116 DNA PAT misc_feature 1..116 note 12b-gRNA2-23 source 1..116 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 114..116 mod_base OTHER note 2'-O-methyl nucleotide modified_base 113..116 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcaccattgagatagtgtggggaaggg 117 DNA PAT misc_feature 1..117 note 12b-gRNA2-24 source 1..117 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 115..117 mod_base OTHER note 2'-O-methyl nucleotide modified_base 114..117 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcaccattgagatagtgtggggaagggg 118 DNA PAT misc_feature 1..118 note 12b-gRNA2-25 source 1..118 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 116..118 mod_base OTHER note 2'-O-methyl nucleotide modified_base 115..118 mod_base OTHER note 2'-O-methyl nucleotide gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcaccattgagatagtgtggggaaggggc 119 DNA PAT misc_feature 1..119 note 12b-gRNA2-26 source 1..119 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 117..119 mod_base OTHER note 2'-O-methyl nucleotide modified_base 116..119 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcaccattgagatagtgtggggaaggggcc 120 DNA PAT misc_feature 1..120 note 12b-gRNA2-27 source 1..120 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 118..120 mod_base OTHER note 2'-O-methyl nucleotide modified_base 117..120 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcaccattgagatagtgtggggaaggggccc 121 DNA PAT misc_feature 1..121 note 12b-gRNA2-28 source 1..121 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 119..121 mod_base OTHER note 2'-O-methyl nucleotide modified_base 118..121 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcaccattgagatagtgtggggaaggggcccc 122 DNA PAT misc_feature 1..122 note 12b-gRNA2-29 source 1..122 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 120..122 mod_base OTHER note 2'-O-methyl nucleotide modified_base 119..122 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcaccattgagatagtgtggggaaggggccccc 123 DNA PAT misc_feature 1..123 note 12b-gRNA2-30 source 1..123 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 121..123 mod_base OTHER note 2'-O-methyl nucleotide modified_base 120..123 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcaccattgagatagtgtggggaaggggccccca 124 DNA PAT misc_feature 1..124 note 12b-gRNA2-31 source 1..124 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 122..124 mod_base OTHER note 2'-O-methyl nucleotide modified_base 121..124 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcaccattgagatagtgtggggaaggggcccccaa 111 DNA PAT misc_feature 1..111 note 12b-gRNA3-18 source 1..111 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 109..111 mod_base OTHER note 2'-O-methyl nucleotide modified_base 108..111 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacgcattgagatagtgtggg 113 DNA PAT misc_feature 1..113 note 12b-gRNA3-20 source 1..113 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 111..113 mod_base OTHER note 2'-O-methyl nucleotide modified_base 110..113 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacgcattgagatagtgtgggga 114 DNA PAT misc_feature 1..114 note 12b-gRNA3-21 source 1..114 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 112..114 mod_base OTHER note 2'-O-methyl nucleotide modified_base 111..114 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacgcattgagatagtgtggggaa 115 DNA PAT misc_feature 1..115 note 12b-gRNA3-22 source 1..115 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 113..115 mod_base OTHER note 2'-O-methyl nucleotide modified_base 112..115 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacgcattgagatagtgtggggaag 116 DNA PAT misc_feature 1..116 note 12b-gRNA3-23 source 1..116 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 114..116 mod_base OTHER note 2'-O-methyl nucleotide modified_base 113..116 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacgcattgagatagtgtggggaagg 117 DNA PAT misc_feature 1..117 note 12b-gRNA3-24 source 1..117 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 115..117 mod_base OTHER note 2'-O-methyl nucleotide modified_base 114..117 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacgcattgagatagtgtggggaaggg 118 DNA PAT misc_feature 1..118 note 12b-gRNA3-25 source 1..118 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 116..118 mod_base OTHER note 2'-O-methyl nucleotide modified_base 115..118 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacgcattgagatagtgtggggaagggg 119 DNA PAT misc_feature 1..119 note 12b-gRNA3-26 source 1..119 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 117..119 mod_base OTHER note 2'-O-methyl nucleotide modified_base 116..119 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacgcattgagatagtgtggggaaggggc 120 DNA PAT misc_feature 1..120 note 12b-gRNA3-27 source 1..120 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 118..120 mod_base OTHER note 2'-O-methyl nucleotide modified_base 117..120 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacgcattgagatagtgtggggaaggggcc 121 DNA PAT misc_feature 1..121 note 12b-gRNA3-28 source 1..121 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 119..121 mod_base OTHER note 2'-O-methyl nucleotide modified_base 118..121 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacgcattgagatagtgtggggaaggggccc 122 DNA PAT misc_feature 1..122 note 12b-gRNA3-29 source 1..122 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 120..122 mod_base OTHER note 2'-O-methyl nucleotide modified_base 119..122 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacgcattgagatagtgtggggaaggggcccc 123 DNA PAT misc_feature 1..123 note 12b-gRNA3-30 source 1..123 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 121..123 mod_base OTHER note 2'-O-methyl nucleotide modified_base 120..123 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacgcattgagatagtgtggggaaggggccccc 124 DNA PAT misc_feature 1..124 note 12b-gRNA3-31 source 1..124 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 122..124 mod_base OTHER note 2'-O-methyl nucleotide modified_base 121..124 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacgcattgagatagtgtggggaaggggccccca 113 DNA PAT misc_feature 1..113 note 12b-gRNA4 source 1..113 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 111..113 mod_base OTHER note 2'-O-methyl nucleotide modified_base 110..113 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacgccagccttgccttgaccaa 113 DNA PAT misc_feature 1..113 note 12b-gRNA5 source 1..113 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 111..113 mod_base OTHER note 2'-O-methyl nucleotide modified_base 110..113 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacccttgtcaaggctattggtc 113 DNA PAT misc_feature 1..113 note 12b-gRNA6 source 1..113 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 111..113 mod_base OTHER note 2'-O-methyl nucleotide modified_base 110..113 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacgccttgtcaaggctattggt 113 DNA PAT misc_feature 1..113 note 12b-gRNA7 source 1..113 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 111..113 mod_base OTHER note 2'-O-methyl nucleotide modified_base 110..113 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcaccagacagatatttgcattga 113 DNA PAT misc_feature 1..113 note 12b-gRNA8 source 1..113 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 111..113 mod_base OTHER note 2'-O-methyl nucleotide modified_base 110..113 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcaccccacactatctcaatgcaa 113 DNA PAT misc_feature 1..113 note 12b-gRNA9 source 1..113 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 111..113 mod_base OTHER note 2'-O-methyl nucleotide modified_base 110..113 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacagacagatatttgcattgag 113 DNA PAT misc_feature 1..113 note 12b-gRNA10 source 1..113 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 111..113 mod_base OTHER note 2'-O-methyl nucleotide modified_base 110..113 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcactcaaggctattggtcaaggc 113 DNA PAT misc_feature 1..113 note 12b-gRNA11 source 1..113 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 111..113 mod_base OTHER note 2'-O-methyl nucleotide modified_base 110..113 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacagcagcagtatcctcttggg 113 DNA PAT misc_feature 1..113 note 12b-gRNA12 source 1..113 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 111..113 mod_base OTHER note 2'-O-methyl nucleotide modified_base 110..113 mod_base OTHER note phosphorothioate internucleotide linkage gcaggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacgtcaagtttgccttgtcaag 107 DNA PAT misc_feature 1..107 note 12b-Aa13tracrRNA source 1..107 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 105..107 mod_base OTHER note 2'-O-methyl nucleotide modified_base 104..107 mod_base OTHER note phosphorothioate internucleotide linkage ggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgaacttctcaaaaagaacgctcgctcagtgttctgac 54 DNA PAT misc_feature 1..54 note 12b-crRNA1-18 source 1..54 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 52..54 mod_base OTHER note 2'-O-methyl nucleotide modified_base 51..54 mod_base OTHER note phosphorothioate internucleotide linkage gtcggatcactgagcgagcgatctgagaagtggcacagatagtgtggggaaggg 56 DNA PAT misc_feature 1..56 note 12b-crRNA1-20 source 1..56 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 54..56 mod_base OTHER note 2'-O-methyl nucleotide modified_base 53..56 mod_base OTHER note phosphorothioate internucleotide linkage gtcggatcactgagcgagcgatctgagaagtggcacagatagtgtggggaaggggc 59 DNA PAT misc_feature 1..59 note 12b-crRNA1-23 source 1..59 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 57..59 mod_base OTHER note 2'-O-methyl nucleotide modified_base 56..59 mod_base OTHER note phosphorothioate internucleotide linkage gtcggatcactgagcgagcgatctgagaagtggcacagatagtgtggggaaggggcccc 61 DNA PAT misc_feature 1..61 note 12b-crRNA1-25 source 1..61 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 59..61 mod_base OTHER note 2'-O-methyl nucleotide modified_base 58..61 mod_base OTHER note phosphorothioate internucleotide linkage gtcggatcactgagcgagcgatctgagaagtggcacagatagtgtggggaaggggccccca 64 DNA PAT misc_feature 1..64 note 12b-crRNA1-28 source 1..64 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 62..64 mod_base OTHER note 2'-O-methyl nucleotide modified_base 61..64 mod_base OTHER note phosphorothioate internucleotide linkage gtcggatcactgagcgagcgatctgagaagtggcacagatagtgtggggaaggggcccccaaga 67 DNA PAT misc_feature 1..67 note 12b-crRNA1-31 source 1..67 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 65..67 mod_base OTHER note 2'-O-methyl nucleotide modified_base 64..67 mod_base OTHER note phosphorothioate internucleotide linkage gtcggatcactgagcgagcgatctgagaagtggcacagatagtgtggggaaggggcccccaagagga 43 DNA PAT misc_feature 1..43 note 12i-gRNA-1 source 1..43 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 41..43 mod_base OTHER note 2'-O-methyl nucleotide modified_base 40..43 mod_base OTHER note phosphorothioate internucleotide linkage agaaatccgtctttcattgacggagatagtgtggggaaggggc 43 DNA PAT misc_feature 1..43 note 12i-gRNA-2 source 1..43 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 41..43 mod_base OTHER note 2'-O-methyl nucleotide modified_base 40..43 mod_base OTHER note phosphorothioate internucleotide linkage agaaatccgtctttcattgacggcattgagatagtgtggggaa 43 DNA PAT misc_feature 1..43 note 12i-gRNA-3 source 1..43 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 41..43 mod_base OTHER note 2'-O-methyl nucleotide modified_base 40..43 mod_base OTHER note phosphorothioate internucleotide linkage agaaatccgtctttcattgacgggcattgagatagtgtgggga 43 DNA PAT misc_feature 1..43 note 12i-gRNA-4 source 1..43 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 41..43 mod_base OTHER note 2'-O-methyl nucleotide modified_base 40..43 mod_base OTHER note phosphorothioate internucleotide linkage agaaatccgtctttcattgacgggccagccttgccttgaccaa 43 DNA PAT misc_feature 1..43 note 12i-gRNA-5 source 1..43 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 41..43 mod_base OTHER note 2'-O-methyl nucleotide modified_base 40..43 mod_base OTHER note phosphorothioate internucleotide linkage agaaatccgtctttcattgacggccttgtcaaggctattggtc 43 DNA PAT misc_feature 1..43 note 12i-gRNA-6 source 1..43 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 41..43 mod_base OTHER note 2'-O-methyl nucleotide modified_base 40..43 mod_base OTHER note phosphorothioate internucleotide linkage agaaatccgtctttcattgacgggccttgtcaaggctattggt 43 DNA PAT misc_feature 1..43 note 12i-gRNA-7 source 1..43 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 41..43 mod_base OTHER note 2'-O-methyl nucleotide modified_base 40..43 mod_base OTHER note phosphorothioate internucleotide linkage agaaatccgtctttcattgacggcagacagatatttgcattga 43 DNA PAT misc_feature 1..43 note 12i-gRNA-8 source 1..43 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 41..43 mod_base OTHER note 2'-O-methyl nucleotide modified_base 40..43 mod_base OTHER note phosphorothioate internucleotide linkage agaaatccgtctttcattgacggcccacactatctcaatgcaa 43 DNA PAT misc_feature 1..43 note 12i-gRNA-9 source 1..43 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 41..43 mod_base OTHER note 2'-O-methyl nucleotide modified_base 40..43 mod_base OTHER note phosphorothioate internucleotide linkage agaaatccgtctttcattgacggagacagatatttgcattgag 43 DNA PAT misc_feature 1..43 note 12i-gRNA-10 source 1..43 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 41..43 mod_base OTHER note 2'-O-methyl nucleotide modified_base 40..43 mod_base OTHER note phosphorothioate internucleotide linkage agaaatccgtctttcattgacggtcaaggctattggtcaaggc 43 DNA PAT misc_feature 1..43 note 12i-gRNA-11 source 1..43 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 41..43 mod_base OTHER note 2'-O-methyl nucleotide modified_base 40..43 mod_base OTHER note phosphorothioate internucleotide linkage agaaatccgtctttcattgacggagcagcagtatcctcttggg 43 DNA PAT misc_feature 1..43 note 12i-gRNA-12 source 1..43 mol_type other DNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 41..43 mod_base OTHER note 2'-O-methyl nucleotide modified_base 40..43 mod_base OTHER note phosphorothioate internucleotide linkage agaaatccgtctttcattgacgggtcaagtttgccttgtcaag 3465 DNA PAT misc_feature 1..3465 note Ecoli Cas12b-M1 source 1..3465 mol_type other DNA organism synthetic construct atgcatcatcatcatcaccatccgaaaaagaaacgcaaagttccgggcatggcagtgaaaagtatgaaagtgaaactgcgtctggataatatgccggaaattcgtgcaggcctgtggaaactgcataccgaagttaatgccggcgttcgctattataccgaatggctgagtctgctgcgtcaggaaaatctgtatcgtcgcagtccgaatggcgatggtgaacaggaatgctataaaaccgccgaagaatgcaaagccgaactgctggaacgtctgcgcgcccgccaggttgaaaatggtcattgcggtccggcaggtagcgatgatgaactgctgcagctggcacgccagctgtatgaactgctggttccgcaggcaattggtgccaaaggtgacgcccagtttattgcccgcaaatttctgagtccgctggccgataaagatgccgtgggtggcctgggtattgccaaagccggcaataagccgcgttgggttcgcatgcgtgaagccggcgaaccgggttgggaagaagaaaaagcaaaagccgaagcacgtaaaagcaccgatcgcaccgcagatgttctgcgtgcactggcagattttggtctgaaaccgctgatgcgtgtttataccgatagcgatatgagcagcgtgcagtggaaaccgctgcgcaaaggccaggccgtgcgcacatgggatcgtgatatgtttcagcaggccattgaacgtatgatgagttgggaaagttggaatcagcgtgtgggtgaagcctatgcaaaactggtggaacagaaaagtcgttttgaacagaaaaatttcgtgggtcaggaacatctggttcagctggtgaatcagctgcagcaggatatgaaagaagcaagccacggtctggaaagcaaagaacagaccgcacattatctgaccggccgtgccctgcgcggcagtgataaagtgtttgaaaaatgggaaaagctggaccctgatgcaccgtttgatctgtatgataccgaaattaagaacgtgcagcgccgtaatacccgccgctttggtagtcatgatctgtttgccaaactggcagaaccgaaatatcaggcactgtggcgcgaagatgccagttttctgacccgttatgccgtttataatagcattgtgcgcaaactgaatcatgccaaaatgtttgccacctttaccctgccggatgcaaccgcacatccgatttggacccgctttgataaactgggtggtaatctgcatcagtatacctttctgtttaatgaatttggtgagggccgtcatgcaattcgctttcagaaactgctgaccgtggaagatggcgtggcaaaagaagtggatgatgtgaccgtgccgattagcatgagcgcacagctggatgatctgctgccgcgtgatccgcatgaactggtggcactgtattttcaggattatggtgcagaacagcatctggccggccgttttggtggtgccaaaattcagtatcgccgtgatcagctgaatcatctgcatgcccgccgtggcgcccgtgatgtttatctgaatctgagcgttcgcgttcagagtcagagcgaagcacgtggtgaacgccgtccgccgtatgcagccgtgtttcgcctggttggcgataatcatcgcgcatttgtgcattttgataaattaagtgactacctggcagaacatccggatgatggtaaactgggtagtgaaggcctgctgagtggcctgcgtgttatgagtgtggatctgggcctgcgtaccagcgcaagtattagtgtgtttcgtgtggcacgtaaagatgaactgaaaccgaatagtgaaggccgcgttccgttttgttttccgattgaaggcaatgaaaatctggttgcagttcatgaacgcagccagctgctgaaactgccgggcgaaaccgaaagcaaagatctgcgcgcaattcgcgaagaacgtcagcgcaccctgcgtcagctgcgtacccagctggcctatctgcgcctgctggtgcgttgtggcagcgaagatgttggccgtcgtgaacgcagctgggcaaaactgattgaacagccgatggatgcaaatcagatgaccccggattggcgcgaagcatttgaagatgaactgcagaaactgaaaagtctgtatggcatttgtggtgaccgtgaatggaccgaagccgtgtatgaaagcgttcgccgcgtttggcgccacatgggcaaacaggttcgcgattggcgcaaagatgttcgtagcggcgaacgtccgaaaattcgtggttatcagaaagatgtggttggcggcaatagcattcgccagattgaatatctggaacgtcagtataaattcctgaaaagttggagctttttcggtaaagtgagtggtcaggttattcgtgccgaaaaaggtagtcgctttgcaattaccctgcgcgaacatattgatcatgccaaagaagatcgtctgaaaaaactggcagatcgtattattatggaagccctgggttatgtgtatgccctggatgatgaacgtggtaaaggcaaatgggtggcaaaatatccgccgtgccagctgattctgctggaagaactgagcgaatatcagtttaataatgatcgcccgccgagtgaaaataatcagctgatgcagtggagccatcgtggtgtgtttcaggaactgctgaatcaggcacaggttcatgatctgctggttggcaccatgtatgcagcctttagtagccgttttgatgcacgtaccggcgcaccgggtattcgttgtcgtcgcgttccggcccgctgcgctcgtgaacagaatccggaaccgtttccgtggtggctgaataagtttgtggccgaacataaactggatggttgtccgctgcgcgcagatgatctgattccgaccggtgaaggtgaatttttcgttagcccgtttagtgccgaagaaggtgactttcatcagattcatgccgatctgaatgccgcacagaatctgcagcgccgtctgtggagcgattttgatattagtcagattcgtctgcgttgtgattggggtgaagtggatggtgaaccggtgctgattccgcgtaccaccggtaaacgcaccgccgatagttatggcaataaggtgttttataccaaaaccggcgttacctattatgaacgtgaacgcggcaaaaaacgtcgcaaagtttttgcccaggaagaactgagtgaagaagaagccgaactgttagtggaagcagatgaagcacgtgaaaaaagcgtggtgctgatgcgcgatccgagtggtattattaatcgcggtgactggacccgtcagaaagaattttggagcatggttaatcagcgcattgaaggttatctggtgaaacagattcgtagccgtgttcgtctgcaggaaagtgcctgtgaaaataccggtgacattgcaagtccgaaaaagaaacgtaaagtttaa 3447 DNA PAT misc_feature 1..3447 note Ecoli Cas12b-M2 source 1..3447 mol_type other DNA organism synthetic construct atgccgaaaaagaaacgcaaagttccgggcatggcagtgaaaagtatgaaagtgaaactgcgtctggataatatgccggaaattcgtgcaggcctgtggaaactgcataccgaagttaatgccggcgttcgctattataccgaatggctgagtctgctgcgtcaggaaaatctgtatcgtcgcagtccgaatggcgatggtgaacaggaatgctataaaaccgccgaagaatgcaaagccgaactgctggaacgtctgcgcgcccgccaggttgaaaatggtcattgcggtccggcaggtagcgatgatgaactgctgcagctggcacgccagctgtatgaactgctggttccgcaggcaattggtgccaaaggtgacgcccagtttattgcccgcaaatttctgagtccgctggccgataaagatgccgtgggtggcctgggtattgccaaagccggcaataagccgcgttgggttcgcatgcgtgaagccggcgaaccgggttgggaagaagaaaaagcaaaagccgaagcacgtaaaagcaccgatcgcaccgcagatgttctgcgtgcactggcagattttggtctgaaaccgctgatgcgtgtttataccgatagcgatatgagcagcgtgcagtggaaaccgctgcgcaaaggccaggccgtgcgcacatgggatcgtgatatgtttcagcaggccattgaacgtatgatgagttgggaaagttggaatcagcgtgtgggtgaagcctatgcaaaactggtggaacagaaaagtcgttttgaacagaaaaatttcgtgggtcaggaacatctggttcagctggtgaatcagctgcagcaggatatgaaagaagcaagccacggtctggaaagcaaagaacagaccgcacattatctgaccggccgtgccctgcgcggcagtgataaagtgtttgaaaaatgggaaaagctggaccctgatgcaccgtttgatctgtatgataccgaaattaagaacgtgcagcgccgtaatacccgccgctttggtagtcatgatctgtttgccaaactggcagaaccgaaatatcaggcactgtggcgcgaagatgccagttttctgacccgttatgccgtttataatagcattgtgcgcaaactgaatcatgccaaaatgtttgccacctttaccctgccggatgcaaccgcacatccgatttggacccgctttgataaactgggtggtaatctgcatcagtatacctttctgtttaatgaatttggtgagggccgtcatgcaattcgctttcagaaactgctgaccgtggaagatggcgtggcaaaagaagtggatgatgtgaccgtgccgattagcatgagcgcacagctggatgatctgctgccgcgtgatccgcatgaactggtggcactgtattttcaggattatggtgcagaacagcatctggccggccgttttggtggtgccaaaattcagtatcgccgtgatcagctgaatcatctgcatgcccgccgtggcgcccgtgatgtttatctgaatctgagcgttcgcgttcagagtcagagcgaagcacgtggtgaacgccgtccgccgtatgcagccgtgtttcgcctggttggcgataatcatcgcgcatttgtgcattttgataaattaagtgactacctggcagaacatccggatgatggtaaactgggtagtgaaggcctgctgagtggcctgcgtgttatgagtgtggatctgggcctgcgtaccagcgcaagtattagtgtgtttcgtgtggcacgtaaagatgaactgaaaccgaatagtgaaggccgcgttccgttttgttttccgattgaaggcaatgaaaatctggttgcagttcatgaacgcagccagctgctgaaactgccgggcgaaaccgaaagcaaagatctgcgcgcaattcgcgaagaacgtcagcgcaccctgcgtcagctgcgtacccagctggcctatctgcgcctgctggtgcgttgtggcagcgaagatgttggccgtcgtgaacgcagctgggcaaaactgattgaacagccgatggatgcaaatcagatgaccccggattggcgcgaagcatttgaagatgaactgcagaaactgaaaagtctgtatggcatttgtggtgaccgtgaatggaccgaagccgtgtatgaaagcgttcgccgcgtttggcgccacatgggcaaacaggttcgcgattggcgcaaagatgttcgtagcggcgaacgtccgaaaattcgtggttatcagaaagatgtggttggcggcaatagcattcgccagattgaatatctggaacgtcagtataaattcctgaaaagttggagctttttcggtaaagtgagtggtcaggttattcgtgccgaaaaaggtagtcgctttgcaattaccctgcgcgaacatattgatcatgccaaagaagatcgtctgaaaaaactggcagatcgtattattatggaagccctgggttatgtgtatgccctggatgatgaacgtggtaaaggcaaatgggtggcaaaatatccgccgtgccagctgattctgctggaagaactgagcgaatatcagtttaataatgatcgcccgccgagtgaaaataatcagctgatgcagtggagccatcgtggtgtgtttcaggaactgctgaatcaggcacaggttcatgatctgctggttggcaccatgtatgcagcctttagtagccgttttgatgcacgtaccggcgcaccgggtattcgttgtcgtcgcgttccggcccgctgcgctcgtgaacagaatccggaaccgtttccgtggtggctgaataagtttgtggccgaacataaactggatggttgtccgctgcgcgcagatgatctgattccgaccggtgaaggtgaatttttcgttagcccgtttagtgccgaagaaggtgactttcatcagattcatgccgatctgaatgccgcacagaatctgcagcgccgtctgtggagcgattttgatattagtcagattcgtctgcgttgtgattggggtgaagtggatggtgaaccggtgctgattccgcgtaccaccggtaaacgcaccgccgatagttatggcaataaggtgttttataccaaaaccggcgttacctattatgaacgtgaacgcggcaaaaaacgtcgcaaagtttttgcccaggaagaactgagtgaagaagaagccgaactgttagtggaagcagatgaagcacgtgaaaaaagcgtggtgctgatgcgcgatccgagtggtattattaatcgcggtgactggacccgtcagaaagaattttggagcatggttaatcagcgcattgaaggttatctggtgaaacagattcgtagccgtgttcgtctgcaggaaagtgcctgtgaaaataccggtgacattgcaagtccgaaaaagaaacgtaaagtttaa 3495 DNA PAT misc_feature 1..3495 note Ecoli Cas12b-M3 source 1..3495 mol_type other DNA organism synthetic construct atgccgaaaaaaaagcgtaaagttccgggcatggcagtgaaaagtatgaaagtgaaactgcgtctggataatatgccggaaattcgtgcaggcctgtggaaactgcataccgaagttaatgccggcgttcgctattataccgaatggctgagtctgctgcgtcaggaaaatctgtatcgtcgcagtccgaatggcgatggtgaacaggaatgctataaaaccgccgaagaatgcaaagccgaactgctggaacgtctgcgcgcccgccaggttgaaaatggtcattgcggtccggcaggtagcgatgatgaactgctgcagctggcacgccagctgtatgaactgctggttccgcaggcaattggtgccaaaggtgacgcccagtttattgcccgcaaatttctgagtccgctggccgataaagatgccgtgggtggcctgggtattgccaaagccggcaataagccgcgttgggttcgcatgcgtgaagccggcgaaccgggttgggaagaagaaaaagcaaaagccgaagcacgtaaaagcaccgatcgcaccgcagatgttctgcgtgcactggcagattttggtctgaaaccgctgatgcgtgtttataccgatagcgatatgagcagcgtgcagtggaaaccgctgcgcaaaggccaggccgtgcgcacatgggatcgtgatatgtttcagcaggccattgaacgtatgatgagttgggaaagttggaatcagcgtgtgggtgaagcctatgcaaaactggtggaacagaaaagtcgttttgaacagaaaaatttcgtgggtcaggaacatctggttcagctggtgaatcagctgcagcaggatatgaaagaagcaagccacggtctggaaagcaaagaacagaccgcacattatctgaccggccgtgccctgcgcggcagtgataaagtgtttgaaaaatgggaaaagctggaccctgatgcaccgtttgatctgtatgataccgaaattaagaacgtgcagcgccgtaatacccgccgctttggtagtcatgatctgtttgccaaactggcagaaccgaaatatcaggcactgtggcgcgaagatgccagttttctgacccgttatgccgtttataatagcattgtgcgcaaactgaatcatgccaaaatgtttgccacctttaccctgccggatgcaaccgcacatccgatttggacccgctttgataaactgggtggtaatctgcatcagtatacctttctgtttaatgaatttggtgagggccgtcatgcaattcgctttcagaaactgctgaccgtggaagatggcgtggcaaaagaagtggatgatgtgaccgtgccgattagcatgagcgcacagctggatgatctgctgccgcgtgatccgcatgaactggtggcactgtattttcaggattatggtgcagaacagcatctggccggccgttttggtggtgccaaaattcagtatcgccgtgatcagctgaatcatctgcatgcccgccgtggcgcccgtgatgtttatctgaatctgagcgttcgcgttcagagtcagagcgaagcacgtggtgaacgccgtccgccgtatgcagccgtgtttcgcctggttggcgataatcatcgcgcatttgtgcattttgataaattaagtgactacctggcagaacatccggatgatggtaaactgggtagtgaaggcctgctgagtggcctgcgtgttatgagtgtggatctgggcctgcgtaccagcgcaagtattagtgtgtttcgtgtggcacgtaaagatgaactgaaaccgaatagtgaaggccgcgttccgttttgttttccgattgaaggcaatgaaaatctggttgcagttcatgaacgcagccagctgctgaaactgccgggcgaaaccgaaagcaaagatctgcgcgcaattcgcgaagaacgtcagcgcaccctgcgtcagctgcgtacccagctggcctatctgcgcctgctggtgcgttgtggcagcgaagatgttggccgtcgtgaacgcagctgggcaaaactgattgaacagccgatggatgcaaatcagatgaccccggattggcgcgaagcatttgaagatgaactgcagaaactgaaaagtctgtatggcatttgtggtgaccgtgaatggaccgaagccgtgtatgaaagcgttcgccgcgtttggcgccacatgggcaaacaggttcgcgattggcgcaaagatgttcgtagcggcgaacgtccgaaaattcgtggttatcagaaagatgtggttggcggcaatagcattcgccagattgaatatctggaacgtcagtataaattcctgaaaagttggagctttttcggtaaagtgagtggtcaggttattcgtgccgaaaaaggtagtcgctttgcaattaccctgcgcgaacatattgatcatgccaaagaagatcgtctgaaaaaactggcagatcgtattattatggaagccctgggttatgtgtatgccctggatgatgaacgtggtaaaggcaaatgggtggcaaaatatccgccgtgccagctgattctgctggaagaactgagcgaatatcagtttaataatgatcgcccgccgagtgaaaataatcagctgatgcagtggagccatcgtggtgtgtttcaggaactgctgaatcaggcacaggttcatgatctgctggttggcaccatgtatgcagcctttagtagccgttttgatgcacgtaccggcgcaccgggtattcgttgtcgtcgcgttccggcccgctgcgctcgtgaacagaatccggaaccgtttccgtggtggctgaataagtttgtggccgaacataaactggatggttgtccgctgcgcgcagatgatctgattccgaccggtgaaggtgaatttttcgttagcccgtttagtgccgaagaaggtgactttcatcagattcatgccgatctgaatgccgcacagaatctgcagcgccgtctgtggagcgattttgatattagtcagattcgtctgcgttgtgattggggtgaagtggatggtgaaccggtgctgattccgcgtaccaccggtaaacgcaccgccgatagttatggcaataaggtgttttataccaaaaccggcgttacctattatgaacgtgaacgcggcaaaaaacgtcgcaaagtttttgcccaggaagaactgagtgaagaagaagccgaactgttagtggaagcagatgaagcacgtgaaaaaagcgtggtgctgatgcgcgatccgagtggtattattaatcgcggtgactggacccgtcagaaagaattttggagcatggttaatcagcgcattgaaggttatctggtgaaacagattcgtagccgtgttcgtctgcaggaaagtgcctgtgaaaataccggtgacattgcaagtcctaaaaagaagcgtaaagtcaaacgtccagcagcaaccaaaaaagcaggccaagcaaaaaaaaaaaagtaa 3474 DNA PAT misc_feature 1..3474 note Ecoli Cas12b-M4 source 1..3474 mol_type other DNA organism synthetic construct atgccgaaaaaaaagcgtaaagttccgggcatggcagtgaaaagtatgaaagtgaaactgcgtctggataatatgccggaaattcgtgcaggcctgtggaaactgcataccgaagttaatgccggcgttcgctattataccgaatggctgagtctgctgcgtcaggaaaatctgtatcgtcgcagtccgaatggcgatggtgaacaggaatgctataaaaccgccgaagaatgcaaagccgaactgctggaacgtctgcgcgcccgccaggttgaaaatggtcattgcggtccggcaggtagcgatgatgaactgctgcagctggcacgccagctgtatgaactgctggttccgcaggcaattggtgccaaaggtgacgcccagtttattgcccgcaaatttctgagtccgctggccgataaagatgccgtgggtggcctgggtattgccaaagccggcaataagccgcgttgggttcgcatgcgtgaagccggcgaaccgggttgggaagaagaaaaagcaaaagccgaagcacgtaaaagcaccgatcgcaccgcagatgttctgcgtgcactggcagattttggtctgaaaccgctgatgcgtgtttataccgatagcgatatgagcagcgtgcagtggaaaccgctgcgcaaaggccaggccgtgcgcacatgggatcgtgatatgtttcagcaggccattgaacgtatgatgagttgggaaagttggaatcagcgtgtgggtgaagcctatgcaaaactggtggaacagaaaagtcgttttgaacagaaaaatttcgtgggtcaggaacatctggttcagctggtgaatcagctgcagcaggatatgaaagaagcaagccacggtctggaaagcaaagaacagaccgcacattatctgaccggccgtgccctgcgcggcagtgataaagtgtttgaaaaatgggaaaagctggaccctgatgcaccgtttgatctgtatgataccgaaattaagaacgtgcagcgccgtaatacccgccgctttggtagtcatgatctgtttgccaaactggcagaaccgaaatatcaggcactgtggcgcgaagatgccagttttctgacccgttatgccgtttataatagcattgtgcgcaaactgaatcatgccaaaatgtttgccacctttaccctgccggatgcaaccgcacatccgatttggacccgctttgataaactgggtggtaatctgcatcagtatacctttctgtttaatgaatttggtgagggccgtcatgcaattcgctttcagaaactgctgaccgtggaagatggcgtggcaaaagaagtggatgatgtgaccgtgccgattagcatgagcgcacagctggatgatctgctgccgcgtgatccgcatgaactggtggcactgtattttcaggattatggtgcagaacagcatctggccggccgttttggtggtgccaaaattcagtatcgccgtgatcagctgaatcatctgcatgcccgccgtggcgcccgtgatgtttatctgaatctgagcgttcgcgttcagagtcagagcgaagcacgtggtgaacgccgtccgccgtatgcagccgtgtttcgcctggttggcgataatcatcgcgcatttgtgcattttgataaattaagtgactacctggcagaacatccggatgatggtaaactgggtagtgaaggcctgctgagtggcctgcgtgttatgagtgtggatctgggcctgcgtaccagcgcaagtattagtgtgtttcgtgtggcacgtaaagatgaactgaaaccgaatagtgaaggccgcgttccgttttgttttccgattgaaggcaatgaaaatctggttgcagttcatgaacgcagccagctgctgaaactgccgggcgaaaccgaaagcaaagatctgcgcgcaattcgcgaagaacgtcagcgcaccctgcgtcagctgcgtacccagctggcctatctgcgcctgctggtgcgttgtggcagcgaagatgttggccgtcgtgaacgcagctgggcaaaactgattgaacagccgatggatgcaaatcagatgaccccggattggcgcgaagcatttgaagatgaactgcagaaactgaaaagtctgtatggcatttgtggtgaccgtgaatggaccgaagccgtgtatgaaagcgttcgccgcgtttggcgccacatgggcaaacaggttcgcgattggcgcaaagatgttcgtagcggcgaacgtccgaaaattcgtggttatcagaaagatgtggttggcggcaatagcattcgccagattgaatatctggaacgtcagtataaattcctgaaaagttggagctttttcggtaaagtgagtggtcaggttattcgtgccgaaaaaggtagtcgctttgcaattaccctgcgcgaacatattgatcatgccaaagaagatcgtctgaaaaaactggcagatcgtattattatggaagccctgggttatgtgtatgccctggatgatgaacgtggtaaaggcaaatgggtggcaaaatatccgccgtgccagctgattctgctggaagaactgagcgaatatcagtttaataatgatcgcccgccgagtgaaaataatcagctgatgcagtggagccatcgtggtgtgtttcaggaactgctgaatcaggcacaggttcatgatctgctggttggcaccatgtatgcagcctttagtagccgttttgatgcacgtaccggcgcaccgggtattcgttgtcgtcgcgttccggcccgctgcgctcgtgaacagaatccggaaccgtttccgtggtggctgaataagtttgtggccgaacataaactggatggttgtccgctgcgcgcagatgatctgattccgaccggtgaaggtgaatttttcgttagcccgtttagtgccgaagaaggtgactttcatcagattcatgccgatctgaatgccgcacagaatctgcagcgccgtctgtggagcgattttgatattagtcagattcgtctgcgttgtgattggggtgaagtggatggtgaaccggtgctgattccgcgtaccaccggtaaacgcaccgccgatagttatggcaataaggtgttttataccaaaaccggcgttacctattatgaacgtgaacgcggcaaaaaacgtcgcaaagtttttgcccaggaagaactgagtgaagaagaagccgaactgttagtggaagcagatgaagcacgtgaaaaaagcgtggtgctgatgcgcgatccgagtggtattattaatcgcggtgactggacccgtcagaaagaattttggagcatggttaatcagcgcattgaaggttatctggtgaaacagattcgtagccgtgttcgtctgcaggaaagtgcctgtgaaaataccggtgacattgcaagtcctaaaaagaagcgtaaagtcccagcagcaaaaaaaaaaaagttagattaa 3546 DNA PAT misc_feature 1..3546 note Ecoli Cas12b-M5 source 1..3546 mol_type other DNA organism synthetic construct atgcctgcagccaaaaagaaaaagctggatggtagcggtagcccgaaaaagaaacgtaaagttcctggtatggcagtcaaaagcatgaaagttaaactgcgcctggataacatgccggaaattcgtgcaggtctgtggaaactgcataccgaagttaatgccggtgttcgttattataccgaatggctgagcctgctgcgtcaagaaaatctgtatcgtcgtagtccgaatggtgatggtgaacaagaatgttataaaaccgcagaagaatgcaaagcagaactgctggaacgtctgcgtgcacgtcaggttgaaaatggtcattgtggtccggcaggtagtgatgatgaactgctgcagctggcacgtcagctgtatgagctgctggttccgcaggcaattggtgcaaaaggtgatgcacagtttattgcccgtaaatttctgagtccgctggcagataaagatgcagttggtggtctgggtattgcaaaagcaggtaataaaccgcgttgggttcgtatgcgtgaagccggtgaacctggttgggaagaagagaaagccaaagccgaagcacgtaaaagcaccgatcgtaccgcagatgttctgcgtgccctggcagattttggtctgaaaccgctgatgcgtgtttataccgatagcgatatgagcagcgttcagtggaaacctctgcgtaaaggtcaggcagttcgtacctgggatcgtgatatgtttcagcaggccattgaacgtatgatgagctgggaaagctggaatcagcgtgttggtgaagcctatgcaaaactggttgaacagaaaagccgtttcgagcagaaaaactttgttggtcaagaacacctggttcagctggtgaatcagctgcaacaggatatgaaagaagcaagccacggtctggaaagcaaagaacagaccgcacattatctgaccggtcgtgcactgcgtggtagcgataaagtttttgaaaaatgggaaaaactggatccggatgcaccgtttgatctgtatgataccgaaatcaaaaatgtgcagcgtcgtaatacccgtcgttttggtagccatgacctgtttgccaaactggcagaaccgaaatatcaggcactgtggcgtgaagatgcaagctttctgacccgttatgcagtgtataatagcattgtgcgcaaactgaatcacgccaaaatgtttgcaacctttacactgccggatgcaaccgcacatccgatttggacccgttttgataaattaggtggtaacctgcaccagtacacctttctgtttaatgaatttggtgaaggtcgtcatgccatccgttttcagaaactgctgaccgttgaagatggtgttgcaaaagaagttgacgacgttaccgttccgattagcatgagcgcacagctggatgatctgcttccgcgtgatccgcatgaactggttgcactgtattttcaggattatggtgcagaacagcatctggcaggtcgctttggtggtgccaaaattcagtatcgtcgcgatcagctgaatcatctgcatgcccgtcgtggtgcacgtgatgtttatctgaatctgagcgttcgtgttcagagccagagcgaagcccgtggtgaacgtcgccctccgtatgcagccgtttttcgtctggttggtgataatcatcgtgcctttgttcactttgataaactgagcgattatctggccgaacatcctgatgatggtaaactgggtagcgaaggtctgctgagcggtctgcgtgttatgagcgttgatctgggtctgcgtaccagcgcaagcattagcgtgtttcgtgttgcacgtaaagatgaactgaaaccgaatagcgaaggccgtgttccgttttgttttccgattgaaggtaatgaaaatctggttgcagtgcatgaacgtagtcagctgctgaaattaccgggtgaaacagaatcaaaagatctgcgtgcgattcgtgaagaacgtcagcgtaccctgcgtcagctgcgcacccagctggcatatctgcgtctgctggtgcgttgtggtagtgaagatgttggtcgtcgtgaacgtagctgggccaaactgatcgaacagccgatggatgcaaatcagatgacaccggattggcgtgaggcatttgaagatgagctgcagaaactgaaaagcctgtatggtatttgtggtgatcgtgaatggaccgaagccgtttatgaaagcgttcgtcgtgtttggcgtcacatgggtaaacaggttcgtgattggcgcaaagatgttcgtagtggcgaacgtccgaaaattcgtggttatcagaaagatgttgttggtggcaatagcattcgccagattgaatatctggaacgccagtacaaattcctgaaaagctggtcatttttcggtaaagttagcggtcaggttattcgtgccgaaaaaggtagccgttttgcaattacactgcgcgaacatattgatcatgccaaagaagatcgtctgaagaaactggccgatcgcattattatggaagcactgggttatgtttatgccctggatgatgaacgtggtaaaggtaaatgggttgccaaatatccgccttgccagctgatcctgctggaagaactgagtgaatatcagtttaataacgatcgtccgcctagcgaaaataaccagctgatgcagtggtcccatcgtggtgtttttcaagaactgttaaatcaggcacaggttcacgatctgctggttggcaccatgtatgcagcatttagcagtcgttttgatgcacgtacaggtgcaccgggtattcgttgccgtcgtgttccggcacgttgtgcccgtgaacagaatccggaaccgtttccgtggtggctgaataaattcgttgcagaacataaactggatggttgtccgctgcgtgccgatgatctgatcccgaccggtgaaggtgaatttttcgttagcccgttttctgccgaagaaggtgattttcatcagattcatgcagatctgaatgcagcacagaatctgcaacgtcgtctgtggtcagattttgatatcagccagattcgtctgcgctgtgattggggtgaagttgatggcgaaccggtgctgattccgcgtaccaccggtaaacgtacagcagatagctatggtaacaaagtgttctataccaaaaccggtgtgacctattatgaacgtgaacgcggtaagaaacgtcgtaaagtgtttgcacaagaagaactgtcagaagaagaagccgaactgttagttgaagcagatgaagcgcgtgaaaaatcagttgttctgatgcgcgatccgagcggtattatcaatcgtggtgattggacacgtcagaaagaattttggagcatggttaatcagcgcattgaaggttatctggtgaaacaaattcgtagccgtgttcgtctgcaagaatcagcatgtgaaaataccggtgatattgcaagccctaagaagaaaagaaaagttggtagtggtagcaaacgtccggcagcaaccaaaaaggcaggtcaggctaaaaagaagaaataa 3525 DNA PAT misc_feature 1..3525 note Ecoli Cas12b-M6 source 1..3525 mol_type other DNA organism synthetic construct atgcctggtatggcagtgaaaagcatgaaagttaaactgcgcctggataacatgccggaaattcgtgcaggtctgtggaaactgcataccgaagttaatgccggtgttcgttattataccgaatggctgagcctgctgcgtcaagaaaatctgtatcgtcgtagcccgaatggtgatggtgaacaagaatgttataaaaccgcagaagaatgcaaagcagaactgctggaacgtctgcgtgcacgtcaggttgaaaatggtcattgtggtccggcaggtagtgatgatgaactgctgcagctggcacgtcagctgtatgagctgctggttccgcaggcaattggtgcaaaaggtgatgcacagtttattgcccgtaaatttctgagtccgctggcagataaagatgcagttggtggtctgggtattgcaaaagcaggtaataaaccgcgttgggttcgtatgcgtgaagccggtgaacctggttgggaagaagaaaaagccaaagccgaagcacgtaaaagcaccgatcgtaccgcagatgttctgcgtgccctggcagattttggtctgaaaccgctgatgcgtgtttataccgatagcgatatgagcagcgttcagtggaaacctctgcgtaaaggtcaggcagttcgtacctgggatcgtgatatgtttcagcaggccattgaacgtatgatgagctgggaaagctggaatcagcgtgttggtgaagcctatgcaaaactggttgaacagaaaagccgtttcgagcagaaaaactttgttggtcaagaacacctggttcagctggtgaatcagctgcaacaggatatgaaagaagcaagccacggtctggaaagcaaagaacagaccgcacattatctgaccggtcgtgcactgcgtggtagcgataaagtttttgaaaaatgggaaaaactggatccggatgcaccgtttgatctgtatgataccgaaatcaaaaatgtgcagcgtcgtaatacccgtcgttttggtagccatgacctgtttgccaaactggcagaaccgaaatatcaggcactgtggcgtgaagatgcaagctttctgacccgttatgcagtgtataatagcattgtgcgcaaactgaatcacgccaaaatgtttgcaacctttacactgccggatgcaaccgcacatccgatttggacccgttttgataaattaggtggtaacctgcaccagtacacctttctgtttaatgaatttggtgaaggtcgtcatgccatccgttttcagaaactgctgaccgttgaagatggtgttgcaaaagaagttgacgacgttaccgttccgattagcatgagcgcacagctggatgatctgcttccgcgtgatccgcatgaactggttgcactgtattttcaggattatggtgcagaacagcatctggcaggtcgctttggtggtgccaaaattcagtatcgtcgcgatcagctgaatcatctgcatgcccgtcgtggtgcacgtgatgtttatctgaatctgagcgttcgtgttcagagccagagcgaagcccgtggtgaacgtcgccctccgtatgcagccgtttttcgtctggttggtgataatcatcgtgcctttgttcactttgataaactgagcgattatctggccgaacatcctgatgatggtaaactgggtagcgaaggtctgctgagcggtctgcgtgttatgagcgttgatctgggtctgcgtaccagcgcaagcattagcgtgtttcgtgttgcacgtaaagatgaactgaaaccgaatagcgaaggccgtgttccgttttgttttccgattgaaggtaatgaaaatctggttgcagtgcatgaacgtagtcagctgctgaaattaccgggtgaaacagaatcaaaagatctgcgtgcgattcgtgaagaacgtcagcgtaccctgcgtcagctgcgcacccagctggcatatctgcgtctgctggtgcgttgtggtagtgaagatgttggtcgtcgtgaacgtagctgggccaaactgatcgaacagccgatggatgcaaatcagatgacaccggattggcgtgaggcatttgaagatgagctgcagaaactgaaaagcctgtatggtatttgtggtgatcgtgaatggaccgaagccgtttatgaaagcgttcgtcgtgtttggcgtcacatgggtaaacaggttcgtgattggcgcaaagatgttcgtagtggcgaacgtccgaaaattcgtggttatcagaaagatgttgttggtggcaatagcattcgccagattgaatatctggaacgccagtacaaattcctgaaaagctggtcatttttcggtaaagttagcggtcaggttattcgtgccgaaaaaggtagccgttttgcaattacactgcgcgaacatattgatcatgccaaagaagatcgtctgaaaaagctggccgatcgcattattatggaagcactgggttatgtttatgccctggatgatgaacgtggtaaaggtaaatgggttgccaaatatccgccttgccagctgatcctgctggaagaactgagtgaatatcagtttaataacgatcgtccgcctagcgaaaataaccagctgatgcagtggtcccatcgtggtgtttttcaagaactgttaaatcaggcacaggttcacgatctgctggttggcaccatgtatgcagcatttagcagtcgttttgatgcacgtacaggtgcaccgggtattcgttgccgtcgtgttccggcacgttgtgcccgtgaacagaatccggaaccgtttccgtggtggctgaataaattcgttgcagaacataaactggatggttgtccgctgcgtgccgatgatctgatcccgaccggtgaaggtgaatttttcgttagcccgttttctgccgaagaaggtgattttcatcagattcatgcagatctgaatgcagcacagaatctgcaacgtcgtctgtggtcagattttgatatcagccagattcgtctgcgctgtgattggggtgaagttgatggcgaaccggtgctgattccgcgtaccaccggtaaacgtacagcagatagctatggtaacaaagtgttctataccaaaaccggtgtgacctattatgaacgtgaacgcggtaagaaacgtcgtaaagtgtttgcacaagaagaactgtcagaagaagaagccgaactgttagttgaagcagatgaagcgcgtgaaaaatcagttgttctgatgcgcgatccgagcggtattatcaatcgtggtgattggacacgtcagaaagaattttggagcatggttaatcagcgcattgaaggttatctggtgaaacaaattcgtagccgtgttcgtctgcaagaaagcgcatgtgaaaataccggtgatattgcaagcccgaaaaagaaacgtaaagttggtagcggtagtccggcagccaaaaagaagaaactggacggtagcggttcaaaacgtcctgcagcaaccaaaaaggcaggtcaggctaaaaagaaaaagtaa 3519 DNA PAT misc_feature 1..3519 note Ecoli Cas12b-M7 source 1..3519 mol_type other DNA organism synthetic construct atgaagcggacggcggacgggagcgaatttgaaagcccgaaaaaaaaacgcaaagttgaaccgggcatggcagtgaaaagtatgaaagtgaaactgcgtctggataatatgccggaaattcgtgcaggcctgtggaaactgcataccgaagttaatgccggcgttcgctattataccgaatggctgagtctgctgcgtcaggaaaatctgtatcgtcgcagtccgaatggcgatggtgaacaggaatgctataaaaccgccgaagaatgcaaagccgaactgctggaacgtctgcgcgcccgccaggttgaaaatggtcattgcggtccggcaggtagcgatgatgaactgctgcagctggcacgccagctgtatgaactgctggttccgcaggcaattggtgccaaaggtgacgcccagtttattgcccgcaaatttctgagtccgctggccgataaagatgccgtgggtggcctgggtattgccaaagccggcaataagccgcgttgggttcgcatgcgtgaagccggcgaaccgggttgggaagaagaaaaagcaaaagccgaagcacgtaaaagcaccgatcgcaccgcagatgttctgcgtgcactggcagattttggtctgaaaccgctgatgcgtgtttataccgatagcgatatgagcagcgtgcagtggaaaccgctgcgcaaaggccaggccgtgcgcacatgggatcgtgatatgtttcagcaggccattgaacgtatgatgagttgggaaagttggaatcagcgtgtgggtgaagcctatgcaaaactggtggaacagaaaagtcgttttgaacagaaaaatttcgtgggtcaggaacatctggttcagctggtgaatcagctgcagcaggatatgaaagaagcaagccacggtctggaaagcaaagaacagaccgcacattatctgaccggccgtgccctgcgcggcagtgataaagtgtttgaaaaatgggaaaagctggaccctgatgcaccgtttgatctgtatgataccgaaattaagaacgtgcagcgccgtaatacccgccgctttggtagtcatgatctgtttgccaaactggcagaaccgaaatatcaggcactgtggcgcgaagatgccagttttctgacccgttatgccgtttataatagcattgtgcgcaaactgaatcatgccaaaatgtttgccacctttaccctgccggatgcaaccgcacatccgatttggacccgctttgataaactgggtggtaatctgcatcagtatacctttctgtttaatgaatttggtgagggccgtcatgcaattcgctttcagaaactgctgaccgtggaagatggcgtggcaaaagaagtggatgatgtgaccgtgccgattagcatgagcgcacagctggatgatctgctgccgcgtgatccgcatgaactggtggcactgtattttcaggattatggtgcagaacagcatctggccggccgttttggtggtgccaaaattcagtatcgccgtgatcagctgaatcatctgcatgcccgccgtggcgcccgtgatgtttatctgaatctgagcgttcgcgttcagagtcagagcgaagcacgtggtgaacgccgtccgccgtatgcagccgtgtttcgcctggttggcgataatcatcgcgcatttgtgcattttgataaattaagtgactacctggcagaacatccggatgatggtaaactgggtagtgaaggcctgctgagtggcctgcgtgttatgagtgtggatctgggcctgcgtaccagcgcaagtattagtgtgtttcgtgtggcacgtaaagatgaactgaaaccgaatagtgaaggccgcgttccgttttgttttccgattgaaggcaatgaaaatctggttgcagttcatgaacgcagccagctgctgaaactgccgggcgaaaccgaaagcaaagatctgcgcgcaattcgcgaagaacgtcagcgcaccctgcgtcagctgcgtacccagctggcctatctgcgcctgctggtgcgttgtggcagcgaagatgttggccgtcgtgaacgcagctgggcaaaactgattgaacagccgatggatgcaaatcagatgaccccggattggcgcgaagcatttgaagatgaactgcagaaactgaaaagtctgtatggcatttgtggtgaccgtgaatggaccgaagccgtgtatgaaagcgttcgccgcgtttggcgccacatgggcaaacaggttcgcgattggcgcaaagatgttcgtagcggcgaacgtccgaaaattcgtggttatcagaaagatgtggttggcggcaatagcattcgccagattgaatatctggaacgtcagtataaattcctgaaaagttggagctttttcggtaaagtgagtggtcaggttattcgtgccgaaaaaggtagtcgctttgcaattaccctgcgcgaacatattgatcatgccaaagaagatcgtctgaaaaaactggcagatcgtattattatggaagccctgggttatgtgtatgccctggatgatgaacgtggtaaaggcaaatgggtggcaaaatatccgccgtgccagctgattctgctggaagaactgagcgaatatcagtttaataatgatcgcccgccgagtgaaaataatcagctgatgcagtggagccatcgtggtgtgtttcaggaactgctgaatcaggcacaggttcatgatctgctggttggcaccatgtatgcagcctttagtagccgttttgatgcacgtaccggcgcaccgggtattcgttgtcgtcgcgttccggcccgctgcgctcgtgaacagaatccggaaccgtttccgtggtggctgaataagtttgtggccgaacataaactggatggttgtccgctgcgcgcagatgatctgattccgaccggtgaaggtgaatttttcgttagcccgtttagtgccgaagaaggtgactttcatcagattcatgccgatctgaatgccgcacagaatctgcagcgccgtctgtggagcgattttgatattagtcagattcgtctgcgttgtgattggggtgaagtggatggtgaaccggtgctgattccgcgtaccaccggtaaacgcaccgccgatagttatggcaataaggtgttttataccaaaaccggcgttacctattatgaacgtgaacgcggcaaaaaacgtcgcaaagtttttgcccaggaagaactgagtgaagaagaagccgaactgttagtggaagcagatgaagcacgtgaaaaaagcgtggtgctgatgcgcgatccgagtggtattattaatcgcggtgactggacccgtcagaaagaattttggagcatggttaatcagcgcattgaaggttatctggtgaaacagattcgtagccgtgttcgtctgcaggaaagtgcctgtgaaaataccggtgacattgcaagtaagcgcaccgcagatggaagcgaatttgaaagcccgaaaaaaaaaagaaaggtggaataa 3546 DNA PAT misc_feature 1..3546 note Ecoli Cas12b-M8 source 1..3546 mol_type other DNA organism synthetic construct atgcccaagaagaagaggaaggttacgcgtatggccgtgaagagtatgaaggtgaagctgcggctggacaacatgcccgagatccgggccggcctgtggaagctgcacaccgaggtgaacgccggcgtgcggtactacaccgagtggctgagcctgctgcggcaggagaacctgtaccggcggagcccaaacggcgacggcgagcaggagtgctacaagaccgccgaggagtgcaaggccgagctgctggagcggctgcgggcccggcaggtggagaacggccactgcggccccgccggcagcgacgacgagctgctgcagctggcccggcagctgtacgagctgctggtgccccaggccatcggcgccaagggcgacgcccagttcatcgcccggaagttcctgagccccctggccgacaaggacgccgtgggcggcctgggcatcgccaaggccggcaacaagccccggtgggtgcggatgcgggaggccggcgagcccggctgggaggaggagaaggccaaggccgaggcccggaagagtaccgaccggaccgccgacgtgctgcgggccctggccgacttcggcctgaagcccctgatgcgggtgtacaccgacagcgacatgagcagcgtgcagtggaagccactgcggaagggccaggccgtgcggacctgggaccgggacatgttccagcaggccatcgagcggatgatgagctgggagagctggaaccagcgggtgggcgaggcctacgccaagctggtggagcagaagagtcggttcgagcagaagaacttcgtgggccaggagcacctggtgcagctggtgaaccagctgcagcaggacatgaaggaggccagccacggcctggagagcaaggagcagaccgcccactacctgaccggccgggccctgcggggcagcgacaaggtgttcgagaagtgggagaagctggaccccgacgcccccttcgacctgtacgacaccgagatcaagaacgtgcagcggcggaacacccggcggttcggcagccacgacctgttcgccaagctggccgagcccaagtaccaggccctgtggcgggaggacgccagcttcctgacccggtacgccgtgtacaacagcatcgtgcggaagctgaaccacgccaagatgttcgccaccttcaccctgcccgacgccaccgcccaccccatctggacccggttcgacaagctgggcggcaacctgcaccagtacaccttcctgttcaacgagttcggcgagggccggcacgccatccggttccagaagctgctgaccgtggaggacggcgtggccaaggaggtggacgacgtgaccgtgcccatcagcatgagcgcccagctggacgacctgctgccccgggacccccacgagctggtggccctgtacttccaggactacggcgccgagcagcacctggccggccggttcggcggcgccaagatccagtaccggcgggaccagctgaaccacctgcacgcccggcggggcgcccgggacgtgtacctgaacctgagcgtgcgggtgcagagccagagcgaggcccggggcgagcggcggccaccctacgccgccgtgttccggctggtgggcgacaaccaccgggccttcgtgcacttcgacaagctgagcgactacctggccgagcacccagacgacggcaagctgggcagcgagggcctgctgagcggcctgcgggtgatgagcgtggacctgggcctgcggaccagcgccagcatcagcgtgttccgggtggcccggaaggacgagctgaagcccaacagcgagggccgggtgcccttctgcttccccatcgagggcaacgagaacctggtggccgtgcacgagcggagccagctgctgaagctgcccggcgaaaccgagagcaaggacctgcgggccatccgggaggagcggcagcggaccctgcggcagctgcggacccagctggcctacctgcggctgctggtgcggtgcggcagcgaggacgtgggccggcgggagcggagctgggccaagctgatcgagcagcccatggacgccaaccagatgacccccgactggcgggaggccttcgaggacgagctgcagaagctgaagagtctgtacggcatctgcggcgaccgggagtggaccgaggccgtgtacgagagcgtgcggcgggtgtggcggcacatgggcaagcaggtgcgggactggcggaaggacgtgcggagcggcgagcggcccaagatccggggctaccagaaggacgtggtgggcggcaacagcatccggcagatcgagtacctggagcggcagtacaagttcctgaagagttggagcttcttcggcaaggtgagcggccaggtgatccgggccgagaagggcagccggttcgccatcaccctgcgggagcacatcgaccacgccaaggaggaccggctgaagaagctggccgaccggatcatcatggaggccctgggctacgtgtacgccctggacgacgagcggggcaagggcaagtgggtggccaagtacccaccctgccagctgatcctgctggaggagctgagcgagtaccagttcaacaacgaccggcccccaagcgagaacaaccagctgatgcagtggagccaccggggcgtgttccaggagctgctgaaccaggcccaggtgcacgacctgctggtgggcaccatgtacgccgccttcagcagccggttcgacgcccggaccggcgcccccggcatccggtgccggcgggtgcccgcccggtgcgcccgggagcagaacccagagcccttcccctggtggctgaacaagttcgtggccgagcacaagctggacggctgccccctgcgggccgacgacctgatccccaccggcgagggcgagttcttcgtgagccccttcagcgccgaggagggcgacttccaccagatccacgccgacctgaacgccgcccagaacctgcagcggcggctgtggagcgacttcgacatcagccagatccggctgcggtgcgactggggcgaggtggacggcgagcccgtgctgatcccccggaccaccggcaagcggaccgccgacagctacggcaacaaggtgttctacaccaagaccggcgtgacctactacgagcgggagcggggcaagaagcggcggaaggtgttcgcccaggaggagctgagcgaggaggaggccgagctgctggtggaggccgacgaggcccgggagaagagtgtggtgctgatgcgggaccccagcggcatcatcaaccggggcgactggacccggcagaaggagttctggagcatggtgaaccagcggatcgagggctacctggtgaagcagatccggagccgggtgcggctgcaggagagcgcctgcgagaacaccggcgacatcgctagcccaaagaagaagaggaaagtcggctctggcagccctgccgccaagaaaaagaagctggacggatctggcagcaagaggcctgctgccaccaagaaggccggccaggccaagaaaaaaaagtga 3240 DNA PAT misc_feature 1..3240 note Cas12i-M1 DNA source 1..3240 mol_type other DNA organism synthetic construct atgcatcatcatcatcaccatcccaagaagaagaggaaggttcccgggatgagcagcgccatcaagagctacaagagcgtgctgcgccccaacgagcgcaagaaccagctgctgaagagcaccatccagtgcctggaggacggcagcgccttcttcttcaagatgctgcagggcctgttcggcggcatcacccccgagatcgtgcgcttcagcaccgagcaggagaagcagcagcaggacatcgccctgtggtgcgccgtgaactggttccgccccgtgagccaggacagcctgacccacaccatcgccagcgacaacctggtggagaagttcgaggagtactacggcggcaccgccagcgacgccatcaagcagtacttcagcgccagcatcggcgagagctactactggaacgactgccgccagcagtactacgacctgtgccgcgagctgggcgtggaggtgagcgacctgacccacgacctggagatcctgtgccgcgagaagtgcctggccgtggccaccgagagcaaccagtacaacagcatcatcagcgtgctgttcggcaccggcagaaaggaggaccgcagcgtgaagctgcgcatcaccaagaagatcctggaggccatcagcaacctgaaggagatccccaagaacgtggcccccatccaggagatcatcctgaacgtggccaaggccaccaaggagaccttccgccaggtgtacgccggcaacctgggcgcccccagcaccctggagagattcatcgccaaggacggccagaaggagttcgacctgaagaagctgcagaccgacctgaagaaggtgatccgcggcaagagcaaggagcgcgactggtgctgccaggaggagctgcgcagctacgtggagcagaacaccatccagtacgacctgtgggcctggggcgagatgttcaacaaggcccacaccgccctgaagatcaagagcacccgcaactacaacttcgccaagcagcgcctggagcagttcaagagaatccagagcctgaacaacctgctggtggtgaagaagctgaacgacttcttcgacagcgagttcttcagcggcgaggagacctacaccatctgcgtgcaccacctgggcggcaagagactgagcaagctgtacaaggcctgggaggacgaccccgccgaccccgagaacgccatcgtggtgctgtgcgacgacctgaagaacaacttcaaggccgagcccatccgcaacatcctgcgctacatcttcaccatccgccaggagtgcagcgcccaggacatcctggccgccgccaagtacaaccagcagctggaccgctacaagagccagaaggccaaccccagcgtgctgggcaaccagggcttcacctggagaaacgccgtgatcctgcccgagaaggcccagcgcaacgaccgccccaacagcctggacctgcgcatctggctgtacctgaagctgcgccaccccgacggccgctggaagaagcaccacatccccttctacgacacccgcttcttccaggagatctacgccgccggcaacagccccgtggacacctgccagttccgcaccccccgcttcggctaccacctgcccaagctgaccgaccagaccgccatccgcgtgaacaagaagcacgtgaaggccgccaagaccgaggcccgcatccgcctggccatccagcagggcaccctgcccgtgagcaacctgaagatcaccagaatcagcgccaccatcaacagcaagggccaggtgcgcatccccgtgaagttcgacgtgggccgccagaagggcaccctgcagatcggcgaccgcttctgcggctacgaccagaaccagaccgccagccacgcctacagcctgtgggaggtggtgaaggagggccagtaccacaaggagctgggctgcttcgtgcgcttcatcagcagcggcgacatcgtgagcatcaccgagaaccgcggcaaccagttcgaccagctgagctacgagggcctggcctacccccagtacgccgactggcgcaagaaggccagcaagttcgtgagcctgtggcagatcaccaagaagaacaagaagaaggagatcgtgaccgtggaggccaaggagaagttcgacgccatctgcaagtaccagccccgcctgtacaagttcaacaaggagtacgcctacctgctgcgcgacatcgtgcgcggcaagagcctggtggagctgcagcagatccgccaggagatcttccgcttcatcgagcaggactgcggcgtgacccgcctgggcagcctgagcctgagcaccctggagaccgtgaaggccgtgaagggcatcatctacagctacttcagcaccgccctgaacgccagcaagaacaaccccatcagcgacgagcagcgcaaggagttcgaccccgagctgttcgccctgctggagaagctggagctgatccgcacccgcaagaagaagcagaaggtggagcgcatcgccaacagcctgatccagacctgcctggagaacaacatcaagttcatccgcggcgagggcgacctgagcaccaccaacaacgccaccaagaagaaggccaacagccgcagcatggactggctggcccgcggcgtgttcaacaagatccgccagctggcccccatgcacaacatcaccctgttcggctgcggcagcctgtacaccagccaccaggaccccctggtgcaccgcaaccccgacaaggccatgaagtgccgctgggccgccatccccgtgaaggacatcggcgactgggtgctgcgcaagctgagccagaacctgcgcgccaagaacatcggcaccggcgagtactaccaccagggcgtgaaggagttcctgagccactacgagctgcaggacctggaggaggagctgctgaagtggcgcagcgaccgcaagagcaacatcccctgctgggtgctgcagaaccgcctggccgagaagctgggcaacaaggaggccgtggtgtacatccccgtgcgcggcggccgcatctacttcgccacccacaaggtggccaccggcgccgtgagcatcgtgttcgaccagaagcaggtgtgggtgtgcaacgccgaccacgtggccgccgccaacatcgccctgaccgtgaagggcatcggcgagcagagcagcgacgaggagaaccccgacggcagccgcatcaagctgcagctgaccagcgctagcccaaagaagaagaggaaagtctag 3240 DNA PAT misc_feature 1..3240 note Cas12i-M2 DNA source 1..3240 mol_type other DNA organism synthetic construct atgcatcatcatcatcaccatcccaagaagaagaggaaggttcccgggatgagcagcgccatcaagagctacaagagcgtgctgcgccccaacgagcgcaagaaccagctgctgaagagcaccatccagtgcctggaggacggcagcgccttcttcttcaagatgctgcagggcctgttcggcggcatcacccccgagatcgtgcgcttcagcaccgagcaggagaagcagcagcaggacatcgccctgtggtgcgccgtgaactggttccgccccgtgagccaggacagcctgacccacaccatcgccagcgacaacctggtggagaagttcgaggagtactacggcggcaccgccagcgacgccatcaagcagtacttcagcgccagcatcggcgagagctactactggaacgactgccgccagcagtactacgacctgtgccgcgagctgggcgtggaggtgagcgacctgacccacgacctggagatcctgtgccgcgagaagtgcctggccgtggccaccgagagcaaccagtacaacagcatcatcagcgtgctgttcggcaccggcagaaaggaggaccgcagcgtgaagctgcgcatcaccaagaagatcctggaggccatcagcaacctgaaggagatccccaagaacgtggcccccatccaggagatcatcctgaacgtggccaaggccaccaaggagaccttccgccaggtgtacgccggcaacctgggcgcccccagcaccctggagagattcatcgccaaggacggccagaaggagttcgacctgaagaagctgcagaccgacctgaagaaggtgatccgcggcaagagcaaggagcgcgactggtgctgccaggaggagctgcgcagctacgtggagcagaacaccatccagtacgacctgtgggcctggggcgagatgttcaacaaggcccacaccgccctgaagatcaagagcacccgcaactacaacttcgccaagcagcgcctggagcagttcaagagaatccagagcctgaacaacctgctggtggtgaagaagctgaacgacttcttcgacagcgagttcttcagcggcgaggagacctacaccatctgcgtgcaccacctgggcggcaagagactgagcaagctgtacaaggcctgggaggacgaccccgccgaccccgagaacgccatcgtggtgctgtgcgacgacctgaagaacaacttcaagaaggagcccatccgcaacatcctgcgctacatcttcaccatccgccaggagtgcagcgcccaggacatcctggccgccgccaagtacaaccagcagctggaccgctacaagagccagaaggccaaccccagcgtgctgggcaaccagggcttcacctggagaaacgccgtgatcctgcccgagaaggcccagcgcaacgaccgccccaacagcctggacctgcgcatctggctgtacctgaagctgcgccaccccgacggccgctggaagaagcaccacatccccttctacgacacccgcttcttccaggagatctacgccgccggcaacagccccgtggacacctgccagttccgcaccccccgcttcggctaccacctgcccaagctgaccgaccagaccgccatccgcgtgaacaagaagcacgtgaaggccgccaagaccgaggcccgcatccgcctggccatccagcagggcaccctgcccgtgagcaacctgaagatcaccagaatcagcgccaccatcaacagcaagggccaggtgcgcatccccgtgaagttcgacgtgggccgccagaagggcaccctgcagatcggcgaccgcttctgcggctacgaccagaaccagaccgccagccacgcctacagcctgtgggaggtggtgaaggagggccagtaccacaaggagctgggctgcttcgtgcgcttcatcagcagcggcgacatcgtgagcatcaccgagaaccgcggcaaccagttcgaccagctgagctacgagggcctggcctacccccagtacgccgactggcgcaagaaggccagcaagttcgtgagcctgtggcagatcaccaagaagaacaagaagaaggagatcgtgaccgtggaggccaaggagaagttcgacgccatctgcaagtaccagccccgcctgtacaagttcaacaaggagtacgcctacctgctgcgcgacatcgtgcgcggcaagagcctggtggagctgcagcagatccgccaggagatcttccgcttcatcgagcaggactgcggcgtgacccgcctgggcagcctgagcctgagcaccctggagaccgtgaaggccgtgaagggcatcatctacagctacttcagcaccgccctgaacgccagcaagaacaaccccatcagcgacgagcagcgcaaggagttcgaccccgagctgttcgccctgctggagaagctggagctgatccgcacccgcaagaagaagcagaaggtggagcgcatcgccaacagcctgatccagacctgcctggagaacaacatcaagttcatccgcggcgagggcgacctgagcaccaccaacaacgccaccaagaagaaggccaacagccgcagcatggactggctggcccgcggcgtgttcaacaagatccgccagctggcccccatgcacaacatcaccctgttcggctgcggcagcctgtacaccagccaccaggaccccctggtgcaccgcaaccccgacaaggccatgaagtgccgctgggccgccatccccgtgaaggacatcggcgactgggtgctgcgcaagctgagccagaacctgcgcgccaagaacatcggcaccggcgagtactaccaccagggcgtgaaggagttcctgagccactacgagctgcaggacctggaggaggagctgctgaagtggcgcagcgaccgcaagagcaacatcccctgctgggtgctgcagaaccgcctggccgagaagctgggcaacaaggaggccgtggtgtacatccccgtgcgcggcggccgcatctacttcgccacccacaaggtggccaccggcgccgtgagcatcgtgttcgaccagaagcaggtgtgggtgtgcaacgccgaccacgtggccgccgccaacatcgccctgaccgtgaagggcatcggcgagcagagcagcgacgaggagaaccccgacggcagccgcatcaagctgcagctgaccagcgctagcccaaagaagaagaggaaagtctag 3216 DNA PAT misc_feature 1..3216 note Cas12i-M3 DNA source 1..3216 mol_type other DNA organism synthetic construct cccaagaagaagaggaaggttcccgggatgagcagcgccatcaagagctacaagagcgtgctgcgccccaacgagcgcaagaaccagctgctgaagagcaccatccagtgcctggaggacggcagcgccttcttcttcaagatgctgcagggcctgttcggcggcatcacccccgagatcgtgcgcttcagcaccgagcaggagaagcagcagcaggacatcgccctgtggtgcgccgtgaactggttccgccccgtgagccaggacagcctgacccacaccatcgccagcgacaacctggtggagaagttcgaggagtactacggcggcaccgccagcgacgccatcaagcagtacttcagcgccagcatcggcgagagctactactggaacgactgccgccagcagtactacgacctgtgccgcgagctgggcgtggaggtgagcgacctgacccacgacctggagatcctgtgccgcgagaagtgcctggccgtggccaccgagagcaaccagtacaacagcatcatcagcgtgctgttcggcaccggcagaaaggaggaccgcagcgtgaagctgcgcatcaccaagaagatcctggaggccatcagcaacctgaaggagatccccaagaacgtggcccccatccaggagatcatcctgaacgtggccaaggccaccaaggagaccttccgccaggtgtacgccggcaacctgggcgcccccagcaccctggagagattcatcgccaaggacggccagaaggagttcgacctgaagaagctgcagaccgacctgaagaaggtgatccgcggcaagagcaaggagcgcgactggtgctgccaggaggagctgcgcagctacgtggagcagaacaccatccagtacgacctgtgggcctggggcgagatgttcaacaaggcccacaccgccctgaagatcaagagcacccgcaactacaacttcgccaagcagcgcctggagcagttcaagagaatccagagcctgaacaacctgctggtggtgaagaagctgaacgacttcttcgacagcgagttcttcagcggcgaggagacctacaccatctgcgtgcaccacctgggcggcaagagactgagcaagctgtacaaggcctgggaggacgaccccgccgaccccgagaacgccatcgtggtgctgtgcgacgacctgaagaacaacttcaagaaggagcccatccgcaacatcctgcgctacatcttcaccatccgccaggagtgcagcgcccaggacatcctggccgccgccaagtacaaccagcagctggaccgctacaagagccagaaggccaaccccagcgtgctgggcaaccagggcttcacctggagaaacgccgtgatcctgcccgagaaggcccagcgcaacgaccgccccaacagcctggacctgcgcatctggctgtacctgaagctgcgccaccccgacggccgctggaagaagcaccacatccccttctacgacacccgcttcttccaggagatctacgccgccggcaacagccccgtggacacctgccagttccgcaccccccgcttcggctaccacctgcccaagctgaccgaccagaccgccatccgcgtgaacaagaagcacgtgaaggccgccaagaccgaggcccgcatccgcctggccatccagcagggcaccctgcccgtgagcaacctgaagatcaccagaatcagcgccaccatcaacagcaagggccaggtgcgcatccccgtgaagttcgacgtgggccgccagaagggcaccctgcagatcggcgaccgcttctgcggctacgaccagaaccagaccgccagccacgcctacagcctgtgggaggtggtgaaggagggccagtaccacaaggagctgggctgcttcgtgcgcttcatcagcagcggcgacatcgtgagcatcaccgagaaccgcggcaaccagttcgaccagctgagctacgagggcctggcctacccccagtacgccgactggcgcaagaaggccagcaagttcgtgagcctgtggcagatcaccaagaagaacaagaagaaggagatcgtgaccgtggaggccaaggagaagttcgacgccatctgcaagtaccagccccgcctgtacaagttcaacaaggagtacgcctacctgctgcgcgacatcgtgcgcggcaagagcctggtggagctgcagcagatccgccaggagatcttccgcttcatcgagcaggactgcggcgtgacccgcctgggcagcctgagcctgagcaccctggagaccgtgaaggccgtgaagggcatcatctacagctacttcagcaccgccctgaacgccagcaagaacaaccccatcagcgacgagcagcgcaaggagttcgaccccgagctgttcgccctgctggagaagctggagctgatccgcacccgcaagaagaagcagaaggtggagcgcatcgccaacagcctgatccagacctgcctggagaacaacatcaagttcatccgcggcgagggcgacctgagcaccaccaacaacgccaccaagaagaaggccaacagccgcagcatggactggctggcccgcggcgtgttcaacaagatccgccagctggcccccatgcacaacatcaccctgttcggctgcggcagcctgtacaccagccaccaggaccccctggtgcaccgcaaccccgacaaggccatgaagtgccgctgggccgccatccccgtgaaggacatcggcgactgggtgctgcgcaagctgagccagaacctgcgcgccaagaacatcggcaccggcgagtactaccaccagggcgtgaaggagttcctgagccactacgagctgcaggacctggaggaggagctgctgaagtggcgcagcgaccgcaagagcaacatcccctgctgggtgctgcagaaccgcctggccgagaagctgggcaacaaggaggccgtggtgtacatccccgtgcgcggcggccgcatctacttcgccacccacaaggtggccaccggcgccgtgagcatcgtgttcgaccagaagcaggtgtgggtgtgcaacgccgaccacgtggccgccgccaacatcgccctgaccgtgaagggcatcggcgagcagagcagcgacgaggagaaccccgacggcagccgcatcaagctgcagctgaccagcgctagcccaaagaagaagaggaaagtc 113 RNA PAT modified_base 3 mod_base OTHER note thymine modified_base 6 mod_base OTHER note thymine modified_base 8 mod_base OTHER note thymine modified_base 10 mod_base OTHER note thymine modified_base 22..26 mod_base OTHER note thymine modified_base 34 mod_base OTHER note thymine modified_base 36 mod_base OTHER note thymine modified_base 42 mod_base OTHER note thymine modified_base 49..51 mod_base OTHER note thymine modified_base 57 mod_base OTHER note thymine modified_base 69..70 mod_base OTHER note thymine modified_base 75..76 mod_base OTHER note thymine modified_base 85 mod_base OTHER note thymine modified_base 94 mod_base OTHER note thymine modified_base 97 mod_base OTHER note thymine modified_base 99 mod_base OTHER note thymine misc_feature 1..113 note 12b-gRNA1-3ex(113) source 1..113 mol_type other RNA organism synthetic construct modified_base 1..3 mod_base OTHER note 2'-O-methyl nucleotide modified_base 1..4 mod_base OTHER note phosphorothioate internucleotide linkage modified_base 111..113 mod_base OTHER note 2'-O-methyl nucleotide modified_base 110..113 mod_base OTHER note phosphorothioate internucleotide linkage ggtcgtctataggacggcgagtttttcaacgggtgtgccaatggccactttccaggtggcaaagcccgttgagcttcaaagaagtggcacagatagtgtggggaaggggcccc 1129 AA PAT REGION 1..1129 note wt AaCas12b source 1..1129 mol_type protein organism synthetic construct MAVKSMKVKLRLDNMPEIRAGLWKLHTEVNAGVRYYTEWLSLLRQENLYRRSPNGDGEQECYKTAEECKAELLERLRARQVENGHCGPAGSDDELLQLARQLYELLVPQAIGAKGDAQQIARKFLSPLADKDAVGGLGIAKAGNKPRWVRMREAGEPGWEEEKAKAEARKSTDRTADVLRALADFGLKPLMRVYTDSDMSSVQWKPLRKGQAVRTWDRDMFQQAIERMMSWESWNQRVGEAYAKLVEQKSRFEQKNFVGQEHLVQLVNQLQQDMKEASHGLESKEQTAHYLTGRALRGSDKVFEKWEKLDPDAPFDLYDTEIKNVQRRNTRREGSHDLFAKLAEPKYQALWREDASFLTRYAVYNSIVRKLNHAKMFATFTLPDATAHPIWTREDKLGGNLHQYTFLENEFGEGRHAIRFQKLLTVEDGVAKEVDDVTVPISMSAQLDDLLPRDPHELVALYFQDYGAEQHLAGEFGGAKIQYRRDQLNHLHARRGARDVYLNLSVRVQSQSEARGERRPPYAAVFRLVGDNHRAFVHFDKLSDYLAEHPDDGKLGSEGLLSGLRVMSVDLGLRTSASISVFRVARKDELKPNSEGRVPFCFPIEGNENLVAVHERSQLLKLPGETESKDLRAIREERQRTLRQLRTQLAYLRLLVRCGSEDVGRRERSWAKLIEQPMDANQMTPDWREAFEDELQKLKSLYGICGDREWTEAVYESVRRVWRHMGKQVRDWRKDVRSGERPKIRGYQKDVVGGNSIEQIEYLERQYKFLKSWSFFGKVSGQVIRAEKGSRFAITLREHIDHAKEDRLKKLADRIIMEALGYVYALDDERGKGKWVAKYPPCQLILLEELSEYQFNNDRPPSENNQLMQWSHRGVFQELLNQAQVHDLLVGTMYAAFSSREDARTGAPGIRCRRVPARCAREQNPEPFPWWLNKFVAEHKLDGCPLRADDLIPTGEGEFFVSPFSAEEGDFHQIHADLNAAQNLQRRLWSDFDISQIRLRCDWGEVDGEPVLIPRTTGKRTADSYGNKVFYTKTGVTYYERERGKKRRKVFAQEELSEEEAELLVEADEAREKSVVLMRDPSGIINRGDWTRQKEFWSMVNQRIEGYLVKQIRSRVRLQESACENTGDI 1054 AA PAT REGION 1..1054 note wt Cas12i2 source 1..1054 mol_type protein organism synthetic construct MSSAIKSYKSVLRPNERKNQLLKSTIQCLEDGSAFFFKMLQGLFGGITPEIVRFSTEQEKQQQDIALWCAVNWFRPVSQDSLTHTIASDNLVEKFEEYYGGTASDAIKQYFSASIGESYYWNDCRQQYYDLCRELGVEVSDLTHDLEILCREKCLAVATESNQNNSIISVLFGTGEKEDRSVKLRITKKILEAISNLKEIPKNVAPIQEIILNVAKATKETFRQVYAGNLGAPSTLEKFIAKDGQKEFDLKKLQTDLKKVIRGKSKERDWCCQEELRSYVEQNTIQYDLWAWGEMFNKAHTALKIKSTRNYNFAKQRLEQFKEIQSLNNLLVVKKLNDFFDSEFFSGEETYTICVHHLGGKDLSKLYKAWEDDPADPENAIVVLCDDLKNNFKKEPIRNILRYIFTIRQECSAQDILAAAKYNQQLDRYKSQKANPSVLGNQGFTWTNAVILPEKAQRNDRPNSLDLRIWLYLKLRHPDGRWKKHHIPFYDTRFFQEIYAAGNSPVDTCQFRTPRFGYHLPKLTDQTAIRVNKKHVKAAKTEARIRLAIQQGTLPVSNLKITEISATINSKGQVRIPVKFDVGRQKGTLQIGDRFCGYDQNQTASHAYSLWEVVKEGQYHKELGCFVRFISSGDIVSITENRGNQFDQLSYEGLAYPQYADWRKKASKFVSLWQITKKNKKKEIVTVEAKEKFDAICKYQPRLYKFNKEYAYLLRDIVRGKSLVELQQIRQEIFRFIEQDCGVTRLGSLSLSTLETVKAVKGIIYSYFSTALNASKNNPISDEQRKEFDPELFALLEKLELIRTRKKKQKVERIANSLIQTCLENNIKFIRGEGDLSTTNNATKKKANSRSMDWLARGVFNKIRQLAPMHNITLFGCGSLYTSHQDPLVHRNPDKAMKCRWAAIPVKDIGDWVLRKLSQNLRAKNIGTGEYYHQGVKEFLSHYELQDLEEELLKWRSDRKSNIPCWVLQNRLAEKLGNKEAVVYIPVRGGRIYFATHKVATGAVSIVFDQKQVWVCNADHVAAANIALTVKGIGEQSSDEENPDGSRIKLQLTS