PCT/KR2023/004709 KR 10-2022-0051454 2022-04-26 LepiDyne Co., Ltd. COMPOSITION FOR DETERMINING WHETHER NUCLEIC ACID IS METHYLATED AND METHOD FOR DETERMINING WHETHER NUCLEIC ACID IS METHYLATED 11 480 DNA PAT misc_feature 1..480 note Nucleic acid to be identified source 1..480 mol_type other DNA organism synthetic construct aggaaggagacggggtggcgccaaggaaggaggagaaaaggcggccgagaaaaggaggagggcaaggggaagaggaagggcgagggaggagcctgaggagactcgcccggctcaaccccgacgtccgcgccccggccgcctgttggccatggcgggcctgggcctgggctccgccgttcccgtgtggctggccgaggacgacctcggctgcatcatctgccaggggctgctggactggcccgccacgctgccctgcggccacagcttctgccgccactgcctggaggccctgtggggcgcccgcgacgcccgccgctgggcctgccccacttgccgccagggcgccgcgcagcagccgcacctgcggaagaacacgctactgcaggacctggccgacaagtaccgccgcgccgcacgcgagatacaggcgggctccgaccctgcccactgcccctgcccgggctccagttccctctccag 480 DNA PAT misc_feature 1..480 note Methylated nucleic acid to be identified treated with sulfite source 1..480 mol_type other DNA organism synthetic construct aggaaggagacggggtggcgttaaggaaggaggagaaaaggcggtcgagaaaaggaggagggtaaggggaagaggaagggcgagggaggagtttgaggagattcgttcggtttaatttcgacgttcgcgtttcggtcgtttgttggttatggcgggtttgggtttgggtttcgtcgttttcgtgtggttggtcgaggacgatttcggttgtattatttgttaggggttgttggattggttcgttacgttgttttgcggttatagtttttgtcgttattgtttggaggttttgtggggcgttcgcgacgttcgtcgttgggtttgttttatttgtcgttagggcgtcgcgtagtagtcgtatttgcggaagaatacgttattgtaggatttggtcgataagtatcgtcgcgtcgtacgcgagatataggcgggtttcgattttgtttattgtttttgttcgggttttagttttttttttag 480 DNA PAT misc_feature 1..480 note Unmethylated nucleic acid to be identified treated with sulfite source 1..480 mol_type other DNA organism synthetic construct aggaaggagatggggtggtgttaaggaaggaggagaaaaggtggttgagaaaaggaggagggtaaggggaagaggaagggtgagggaggagtttgaggagatttgtttggtttaattttgatgtttgtgttttggttgtttgttggttatggtgggtttgggtttgggttttgttgtttttgtgtggttggttgaggatgattttggttgtattatttgttaggggttgttggattggtttgttatgttgttttgtggttatagtttttgttgttattgtttggaggttttgtggggtgtttgtgatgtttgttgttgggtttgttttatttgttgttagggtgttgtgtagtagttgtatttgtggaagaatatgttattgtaggatttggttgataagtattgttgtgttgtatgtgagatataggtgggttttgattttgtttattgtttttgtttgggttttagttttttttttag 25 DNA PAT misc_feature 1..25 note Methylation primer FWD source 1..25 mol_type other DNA organism synthetic construct ttatggcgggtttgggtttgggttt 25 DNA PAT misc_feature 1..25 note Unmethylation primer FWD source 1..25 mol_type other DNA organism synthetic construct ttatggtgggtttgggtttgggttt 24 DNA PAT misc_feature 1..24 note common REV primer source 1..24 mol_type other DNA organism synthetic construct caacccctaacaaataatacaacc 24 DNA PAT misc_feature 1..24 note methylation independent primer FWD source 1..24 mol_type other DNA organism synthetic construct gttgtattatttgttaggggttgt 22 DNA PAT misc_feature 1..22 note methylation independent primer RVS source 1..22 mol_type other DNA organism synthetic construct ccacaaaacctccaaacaataa 26 DNA PAT misc_feature 1..26 note primer with CpG recognition site FWD source 1..26 mol_type other DNA organism synthetic construct tttcggttgtattatttgttaggggt 22 DNA PAT misc_feature 1..22 note primer with CpG recognition site RVS source 1..22 mol_type other DNA organism synthetic construct aacgccccacaaaacctccaaa 18 DNA PAT misc_feature 1..18 note Fluorescent probe source 1..18 mol_type other DNA organism synthetic construct ggttcgttacgttgtttt