0245-PA-020 CN 2023109693207 2023-08-03 Shanghai QuietD Biotechnology Co., Ltd. Shanghai QuietD Biotechnology Co., Ltd. LI Kaicheng USE OF PROTON PUMP REGULATOR IN PREPARATION OF DRUG 73 19 AA PAT REGION 1..19 note QD202 source 1..19 mol_type protein organism synthetic construct YGRKKRRQRRRSPVDVVCS 18 AA PAT REGION 1..18 note QD202 N -1 source 1..18 mol_type protein organism synthetic construct YGRKKRRQRRRPVDVVCS 18 AA PAT REGION 1..18 note QD202 C -1 source 1..18 mol_type protein organism synthetic construct YGRKKRRQRRRSPVDVVC 17 AA PAT REGION 1..17 note QD202 C -2 source 1..17 mol_type protein organism synthetic construct YGRKKRRQRRRSPVDVV 19 AA PAT REGION 1..19 note QD202 variant SITE 19 note misc_feature - Xaa = Ser or not exist source 1..19 mol_type protein organism synthetic construct YGRKKRRQRRRSPVDVVCX 775 AA PAT REGION 1..775 note ATP6V1B2-mCherry fusion protein source 1..775 mol_type protein organism synthetic construct MALRAMRGIVNGAAPELPVPTGGPMAGAREQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAEIVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPIDRGPVVLAEDFLDIMGQPINPQCRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFEKNFITQGPYENRTVYETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSAKHEQKLISEEDLATNFSLLKQAGDVEENPGPVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK 19 AA PAT REGION 1..19 note Shuffled QD201 as control source 1..19 mol_type protein organism synthetic construct YGRKKRRQRRRVCDSSVVP 511 AA PAT source 1..511 mol_type protein organism Mus musculus MALRAMRGIVNGAAPELPVPTGGPMAGAREQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAEIVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPIDRGPVVLAEDFLDIMGQPINPQCRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFEKNFITQGPYENRTVYETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSAKH 1536 DNA PAT source 1..1536 mol_type genomic DNA organism Mus musculus misc_feature 1..1536 note Mouse ATP6V1B2 DNA atggcgttgcgagcgatgcggggaatcgtgaacggggccgcacccgaactgcccgtgcccaccggtgggccgatggccggagctcgggagcaggcgctggcggtgagccggaactacctatcccagcctcgtctcacctacaagactgtctctggggtgaatggtccactagtgatcctagatcatgtgaagtttcccagatacgctgagattgtccacttgacattaccagatggcacaaagagaagtgggcaagtcctagaagttagtggctccaaagcagtggttcaggtatttgaagggacatctggtatagacgccaagaaaacatcctgtgagtttactggagatattctccgaacaccagtgtctgaggatatgcttggtcgagtattcaacggatcaggaaaacccattgaccgaggccctgtggtgctggctgaagacttccttgacatcatgggtcagccaatcaaccctcagtgtcggatctatccagaagagatgattcagacgggcatttccgccatcgatggcatgaacagtattgctaggggacagaaaatccccatcttttctgctgctggattaccacacaacgagattgcagctcagatctgtcgccaggctggtttggtaaagaaatccaaagatgtagtggactatagtgaagaaaattttgccattgtgtttgctgctatgggagtaaacatggaaacagcccggttctttaaatctgactttgaagaaaatggctcaatggacaatgtctgcctttttttgaatctggctaatgacccaaccattgagcggatcatcactcctcgcctggctctgaccacggccgagtttctggcatatcagtgtgagaagcacgtgctggtgatcctgacggacatgagctcctacgctgaagcgctgcgagaggtttcagctgccagggaagaggtccctggtcggcgaggcttcccaggctacatgtataccgacttagccacaatctatgaacgtgccggtcgagtggaaggtagaaacggctctattacccaaatccctattctcaccatgcccaatgacgatatcactcatcccatccctgacttgactgggtacattactgagggccagatctatgtggacagacagctgcacaacagacagatttaccctcctattaatgtgctgccctcactctctcggttaatgaagtcagctatcggagaaggaatgaccagaaaggatcacgctgatgtgtctaaccagttgtatgcgtgctatgccatcggtaaggacgtgcaagccatgaaagccgtggtgggagaggaagccctgacctcagatgatctcctttacctggaatttctgcagaagtttgagaagaacttcattactcagggtccctatgaaaaccgaactgtttatgagactttggacattggctggcagttgcttcgaatcttccccaaagaaatgctgaagagaatccctcagagcaccctgagcgaattttaccctcgagactctgcaaaacactag 286 AA PAT REGION 1..286 note Mouse ATP6V1B2 1-286aa truncated sequence source 1..286 mol_type protein organism synthetic construct MALRAMRGIVNGAAPELPVPTGGPMAGAREQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAEIVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPIDRGPVVLAEDFLDIMGQPINPQCRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLA 172 AA PAT REGION 1..172 note Mouse ATP6V1B2 1-172aa truncated sequence source 1..172 mol_type protein organism synthetic construct MALRAMRGIVNGAAPELPVPTGGPMAGAREQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAEIVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPIDRGPVVLAEDFLDIMGQPINPQCRIYPEEMIQT 550 AA PAT REGION 1..550 note Mouse ATP6V1B2 fusion with Myc protein source 1..550 mol_type protein organism synthetic construct MALRAMRGIVNGAAPELPVPTGGPMAGAREQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAEIVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPIDRGPVVLAEDFLDIMGQPINPQCRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFEKNFITQGPYENRTVYETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSAKHEFCRYPAQWRPLESRGPFEQKLISEEDLNMHTGHHHHHH 325 AA PAT REGION 1..325 note Mouse ATP6V1B2 1-286aa fusion with Myc protein source 1..325 mol_type protein organism synthetic construct MALRAMRGIVNGAAPELPVPTGGPMAGAREQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAEIVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPIDRGPVVLAEDFLDIMGQPINPQCRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAEFCRYPAQWRPLESRGPFEQKLISEEDLNMHTGHHHHHH 211 AA PAT REGION 1..211 note MouseATP6V1B2 1-172aa truncated sequence fusion with Myc protein source 1..211 mol_type protein organism synthetic construct MALRAMRGIVNGAAPELPVPTGGPMAGAREQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAEIVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPIDRGPVVLAEDFLDIMGQPINPQCRIYPEEMIQTEFCRYPAQWRPLESRGPFEQKLISEEDLNMHTGHHHHHH 269 AA PAT REGION 1..269 note QD202-EGFP fusion protein source 1..269 mol_type protein organism synthetic construct MYGRKKRRQRRRSPVDVVCSGTAGPGSIATMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK 511 AA PAT source 1..511 mol_type protein organism Homo sapiens REGION 1..511 note Human ATP6V1B2 MALRAMRGIVNGAAPELPVPTGGPAVGAREQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAEIVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPIDRGPVVLAEDFLDIMGQPINPQCRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFERNFIAQGPYENRTVFETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSAKH 1536 DNA PAT source 1..1536 mol_type genomic DNA organism Homo sapiens misc_feature 1..1536 note Human ATP6V1B2 DNA atggcgctgcgggcgatgcgggggattgtcaacggggccgcacccgagctacccgtgcccaccggtgggccggcggtgggagctcgggagcaggcgctggcagtcagtcggaactacctctcccagcctcgcctcacatacaagacagtatctggagtcaatggtccactagtgatcttagatcatgttaagtttcccaggtatgctgaaattgtccatttgaccttaccggatggcacaaagagaagtgggcaagttctggaagttagtggttccaaggcagtagttcaggtatttgaagggacttcaggtatagatgctaagaaaacgtcctgtgagtttactggggatattctccgaacaccggtgtctgaggatatgcttggtcgggtattcaatggatcgggaaaacccattgacagaggtcctgttgtactggccgaagacttccttgatatcatgggtcagccaatcaaccctcaatgtcgaatctacccagaggaaatgattcagactggcatttcggccatcgatgggatgaacagtattgctagggggcagaaaattcctatcttctctgctgctgggctaccacacaatgagattgcagctcagatctgtcgccaggctggtttggtaaagaaatccaaagatgtagtagactacagtgaggaaaattttgcaattgtatttgctgctatgggtgtaaacatggaaactgcccggttcttcaaatctgactttgaagaaaatggctcaatggacaatgtctgcctctttttgaacttggctaatgacccaaccattgagcgaattatcactcctcgcctggctctaaccacagctgaatttctggcgtaccaatgtgagaaacatgtattggttattctaacagacatgagttcttatgctgaagcacttcgagaggtttcagcagccagggaagaggtacctggtcgacgaggttttccaggttacatgtatacagatttagccacgatatatgaacgcgctgggcgagtggaagggagaaacggctcgattactcaaatccctattctaaccatgcctaatgatgatatcactcaccccatcccagacttgactggctacattacagaggggcagatctatgtggacagacagctgcacaacagacagatttatccacctatcaatgtgctgccctcactatcacggttaatgaagtctgctattggagaagggatgaccaggaaggatcatgccgatgtatctaaccagctatatgcgtgctatgctattggaaaggatgtgcaagccatgaaagctgtcgttggagaagaagcccttacctcagatgatcttctctacttggaatttctgcagaagtttgagaggaacttcattgctcagggtccttacgaaaatcgcactgtctttgagactttggacattggctggcagctactccgaatcttccccaaagaaatgctgaagagaatccctcagagcaccctcagcgaattttaccctcgagactctgcaaagcattag 38 AA PAT REGION 1..38 note QD202 dimer source 1..38 mol_type protein organism synthetic construct YGRKKRRQRRRSPVDVVCSSCVVDVPSRRRQRRKKRGY 8 AA PAT REGION 1..8 note ATP6V1B2 binding region of QD202 SITE 8 note misc_feature - Xaa = Ser or not exist source 1..8 mol_type protein organism synthetic construct SPVDVVCX 8 AA PAT REGION 1..8 note Mutation of the second amino acid in ATP6V1B2 binding region to alanine SITE 8 note misc_feature - Xaa = Ser or not exist source 1..8 mol_type protein organism synthetic construct SAVDVVCX 8 AA PAT REGION 1..8 note Mutation of the third amino acid in ATP6V1B2 binding region to alanine SITE 8 note misc_feature - Xaa = Ser or not exist source 1..8 mol_type protein organism synthetic construct SPADVVCX 8 AA PAT REGION 1..8 note Mutation of the fourth amino acid in ATP6V1B2 binding region to alanine SITE 8 note misc_feature - Xaa = Ser or not exist source 1..8 mol_type protein organism synthetic construct SPVAVVCX 8 AA PAT REGION 1..8 note Mutation of the second and the third amino acid in ATP6V1B2 binding region to alanine SITE 8 note misc_feature - Xaa = Ser or not exist source 1..8 mol_type protein organism synthetic construct SAADVVCX 8 AA PAT REGION 1..8 note Mutation of the second and the fourth amino acid in ATP6V1B2 binding region to alanine SITE 8 note misc_feature - Xaa = Ser or not exist source 1..8 mol_type protein organism synthetic construct SAVAVVCX 8 AA PAT REGION 1..8 note Mutation of the third and the fourth amino acid in ATP6V1B2 binding region to alanine SITE 8 note misc_feature - Xaa = Ser or not exist source 1..8 mol_type protein organism synthetic construct SPAAVVCX 8 AA PAT REGION 1..8 note Mutation of the second, the third and the fourth amino acid in ATP6V1B2 binding region to alanine SITE 8 note misc_feature - Xaa = Ser or not exist source 1..8 mol_type protein organism synthetic construct SAAAVVCX 8 AA PAT source 1..8 mol_type protein organism synthetic construct SITE 8 note note = misc_feature - Xaa = Ser or not exist REGION 1..8 note Mutation of the second amino acid in ATP6V1B2 binding region SITE 2 note note = misc_feature - Xaa = any amino acid SXVDVVCX 8 AA PAT source 1..8 mol_type protein organism synthetic construct SITE 8 note note = misc_feature - Xaa = Ser or not exist REGION 1..8 note Mutation of the third amino acid in ATP6V1B2 binding region SITE 3 note note = misc_feature - Xaa = any amino acid SPXDVVCX 8 AA PAT source 1..8 mol_type protein organism synthetic construct SITE 8 note note = misc_feature - Xaa = Ser or not exist REGION 1..8 note Mutation of the fourth amino acid in ATP6V1B2 binding region SITE 4 note note = misc_feature - Xaa = any amino acid SPVXVVCX 8 AA PAT source 1..8 mol_type protein organism synthetic construct SITE 8 note note = misc_feature - Xaa = Ser or not exist REGION 1..8 note Mutation of the second and the third amino acid in ATP6V1B2 binding region SITE 2 note note = misc_feature - Xaa = any amino acid SITE 3 note note = misc_feature - Xaa = any amino acid SXXDVVCX 8 AA PAT source 1..8 mol_type protein organism synthetic construct SITE 8 note note = misc_feature - Xaa = Ser or not exist REGION 1..8 note Mutation of the second and the fourth amino acid in ATP6V1B2 binding region SITE 2 note note = misc_feature - Xaa = any amino acid SITE 4 note note = misc_feature - Xaa = any amino acid SXVXVVCX 8 AA PAT source 1..8 mol_type protein organism synthetic construct SITE 8 note note = misc_feature - Xaa = Ser or not exist REGION 1..8 note Mutation of the third and the fourth amino acid in ATP6V1B2 binding region SITE 3 note note = misc_feature - Xaa = any amino acid SITE 4 note note = misc_feature - Xaa = any amino acid SPXXVVCX 8 AA PAT source 1..8 mol_type protein organism synthetic construct SITE 8 note note = misc_feature - Xaa = Ser or not exist REGION 1..8 note Mutation of the second, the third and the fourth amino acid in ATP6V1B2 binding region SITE 2 note note = misc_feature - Xaa = any amino acid SITE 3 note note = misc_feature - Xaa = any amino acid SITE 4 note note = misc_feature - Xaa = any amino acid SXXXVVCX 11 AA PAT source 1..11 mol_type protein organism synthetic construct REGION 1..8 note Cell penetrating peptide YGRKKRRQRRR 19 AA PAT source 1..19 mol_type protein organism synthetic construct REGION 1..19 note Mutation of the second amino acid in ATP6V1B2 binding region to alanine fusion with cell penetrating peptide SITE 19 note note = misc_feature - Xaa = Ser or not exist YGRKKRRQRRRSAVDVVCX 19 AA PAT source 1..19 mol_type protein organism synthetic construct SITE 19 note note = misc_feature - Xaa = Ser or not exist REGION 1..19 note Mutation of the third amino acid in ATP6V1B2 binding region to alanine fusion with cell penetrating peptide YGRKKRRQRRRSPADVVCX 19 AA PAT source 1..19 mol_type protein organism synthetic construct SITE 19 note note = misc_feature - Xaa = Ser or not exist REGION 1..19 note Mutation of the fourth amino acid in ATP6V1B2 binding region to alanine fusion with cell penetrating peptide YGRKKRRQRRRSPVAVVCX 19 AA PAT source 1..19 mol_type protein organism synthetic construct SITE 19 note note = misc_feature - Xaa = Ser or not exist REGION 1..19 note Mutation of the second and the third amino acid in ATP6V1B2 binding region to alanine fusion with cell penetrating peptide YGRKKRRQRRRSAADVVCX 19 AA PAT source 1..19 mol_type protein organism synthetic construct SITE 19 note note = misc_feature - Xaa = Ser or not exist REGION 1..19 note Mutation of the second and the fourth amino acid in ATP6V1B2 binding region to alanine fusion with cell penetrating peptide YGRKKRRQRRRSAVAVVCX 19 AA PAT source 1..19 mol_type protein organism synthetic construct SITE 19 note note = misc_feature - Xaa = Ser or not exist REGION 1..19 note Mutation of the third and the fourth amino acid in ATP6V1B2 binding region to alanine fusion with cell penetrating peptide YGRKKRRQRRRSPAAVVCX 19 AA PAT source 1..19 mol_type protein organism synthetic construct SITE 19 note note = misc_feature - Xaa = Ser or not exist REGION 1..19 note Mutation of the second, the third and the fourth amino acid in ATP6V1B2 binding region to alanine fusion with cell penetrating peptide YGRKKRRQRRRSAAAVVCX 19 AA PAT source 1..19 mol_type protein organism synthetic construct SITE 13 note misc_feature - Xaa = any amino acid SITE 19 note note = misc_feature - Xaa = Ser or not exist REGION 1..19 note Mutation of the second amino acid in ATP6V1B2 binding region fusion with cell penetrating peptide YGRKKRRQRRRSXVDVVCX 19 AA PAT source 1..19 mol_type protein organism synthetic construct SITE 19 note note = misc_feature - Xaa = Ser or not exist SITE 14 note note = misc_feature - Xaa = any amino acid REGION 1..19 note Mutation of the second, the third and the fourth amino acid in ATP6V1B2 binding region to alanine fusion with cell penetrating peptide YGRKKRRQRRRSPXDVVCX 19 AA PAT source 1..19 mol_type protein organism synthetic construct SITE 15 note note = misc_feature - Xaa = any amino acid SITE 19 note note = misc_feature - Xaa = Ser or not exist REGION 1..19 note Mutation of the fourth amino acid in ATP6V1B2 binding region fusion with cell penetrating peptide YGRKKRRQRRRSPVXVVCX 19 AA PAT source 1..19 mol_type protein organism synthetic construct SITE 13 note note = misc_feature - Xaa = any amino acid SITE 14 note note = misc_feature - Xaa = any amino acid REGION 1..19 note Mutation of the second and the third amino acid in ATP6V1B2 binding region fusion with cell penetrating peptide YGRKKRRQRRRSXXDVVCX 19 AA PAT source 1..19 mol_type protein organism synthetic construct SITE 19 note note = misc_feature - Xaa = Ser or not exist SITE 13 note note = misc_feature - Xaa = any amino acid SITE 15 note note = misc_feature - Xaa = any amino acid REGION 1..19 note Mutation of the second, the third and the fourth amino acid in ATP6V1B2 binding region to alanine fusion with cell penetrating peptide YGRKKRRQRRRSXVXVVCX 19 AA PAT source 1..19 mol_type protein organism synthetic construct SITE 14 note note = misc_feature - Xaa = any amino acid SITE 15 note note = misc_feature - Xaa = any amino acid SITE 19 note note = misc_feature - Xaa = Ser or not exist REGION 1..19 note Mutation of the third and the fourth amino acid in ATP6V1B2 binding region fusion with cell penetrating peptide YGRKKRRQRRRSPXXVVCX 19 AA PAT source 1..19 mol_type protein organism synthetic construct SITE 13 note note = misc_feature - Xaa = any amino acid SITE 15 note note = misc_feature - Xaa = any amino acid SITE 14 note note = misc_feature - Xaa = any amino acid SITE 19 note note = misc_feature - Xaa = Ser or not exist REGION 1..19 note Mutation of the second, the third and the fourth amino acid in ATP6V1B2 binding region fusion with cell penetrating peptide YGRKKRRQRRRSXXXVVCX 1533 DNA PAT source 1..1533 mol_type other DNA organism synthetic construct misc_feature 1..1533 note hATP6V1B2 plasmid atggcgctgcgggcgatgcgggggattgtcaacggggccgcacccgagctacccgtgcccaccggtgggccggcggtgggagctcgggagcaggcgctggcagtcagtcggaactacctctcccagcctcgcctcacatacaagacagtatctggagtcaatggtccactagtgatcttagatcatgttaagtttcccaggtatgctgaaattgtccatttgaccttaccggatggcacaaagagaagtgggcaagttctggaagttagtggttccaaggcagtagttcaggtatttgaagggacttcaggtatagatgctaagaaaacgtcctgtgagtttactggggatattctccgaacaccggtgtctgaggatatgcttggtcgggtattcaatggatcgggaaaacccattgacagaggtcctgttgtactggccgaagacttccttgatatcatgggtcagccaatcaaccctcaatgtcgaatctacccagaggaaatgattcagactggcatttcggccatcgatgggatgaacagtattgctagggggcagaaaattcctatcttctctgctgctgggctaccacacaatgagattgcagctcagatctgtcgccaggctggtttggtaaagaaatccaaagatgtagtagactacagtgaggaaaattttgcaattgtatttgctgctatgggtgtaaacatggaaactgcccggttcttcaaatctgactttgaagaaaatggctcaatggacaatgtctgcctctttttgaacttggctaatgacccaaccattgagcgaattatcactcctcgcctggctctaaccacagctgaatttctggcgtaccaatgtgagaaacatgtattggttattctaacagacatgagttcttatgctgaagcacttcgagaggtttcagcagccagggaagaggtacctggtcgacgaggttttccaggttacatgtatacagatttagccacgatatatgaacgcgctgggcgagtggaagggagaaacggctcgattactcaaatccctattctaaccatgcctaatgatgatatcactcaccccatcccagacttgactggctacattacagaggggcagatctatgtggacagacagctgcacaacagacagatttatccacctatcaatgtgctgccctcactatcacggttaatgaagtctgctattggagaagggatgaccaggaaggatcatgccgatgtatctaaccagctatatgcgtgctatgctattggaaaggatgtgcaagccatgaaagctgtcgttggagaagaagcccttacctcagatgatcttctctacttggaatttctgcagaagtttgagaggaacttcattgctcagggtccttacgaaaatcgcactgtctttgagactttggacattggctggcagctactccgaatcttccccaaagaaatgctgaagagaatccctcagagcaccctcagcgaattttaccctcgagactctgcaaagcat 60 DNA PAT source 1..60 mol_type other DNA organism synthetic construct misc_feature 1..60 note QD202(D15A) DNA sequence atgtacggcaggaagaagaggaggcagaggaggaggagccccgtggccgtggtgtgcagc 60 DNA PAT misc_feature 1..60 note QD202(V16A) DNA sequence source 1..60 mol_type other DNA organism synthetic construct atgtacggcaggaagaagaggaggcagaggaggaggagccccgtggacgccgtgtgcagc 60 DNA PAT misc_feature 1..60 note QD202(P13A) DNA sequence source 1..60 mol_type other DNA organism synthetic construct atgtacggcaggaagaagaggaggcagaggaggaggagcgccgtggacgtggtgtgcagc 60 DNA PAT misc_feature 1..60 note QD202(V14A) DNA sequence source 1..60 mol_type other DNA organism synthetic construct atgtacggcaggaagaagaggaggcagaggaggaggagccccgccgacgtggtgtgcagc 8 AA PAT REGION 1..8 note ATP6V1B2 binding region of QD202 with 8AA source 1..8 mol_type protein organism synthetic construct SPVDVVCS 7 AA PAT REGION 1..7 note ATP6V1B2 binding region of QD202 with 7AA source 1..7 mol_type protein organism synthetic construct SPVDVVC 84 AA PAT source 1..84 mol_type protein organism synthetic construct REGION 1..84 note DIR MVATGSLSSKNPASISELLDCGYHPESLLSGECPRCPPFSPPKVGASGSIGWAGFRLPPLPLCRRCHKIHLRRLLSKFSNIFSP 30 AA PAT source 1..30 mol_type protein organism synthetic construct MVATGSLSSKNPASISELLDCGYHPESLLS 54 AA PAT source 1..54 mol_type protein organism synthetic construct GECPRCPPFSPPKVGASGSIGWAGFRLPPLPLCRRCHKIHLRRLLSKFSNIFSP 27 AA PAT source 1..27 mol_type protein organism synthetic construct GECPRCPPFSPPKVGASGSIGWAGFRL 27 AA PAT source 1..27 mol_type protein organism synthetic construct PPLPLCRRCHKIHLRRLLSKFSNIFSP 20 DNA PAT source 1..20 mol_type other DNA organism synthetic construct misc_feature 1..20 note forward primer tgctggactgtggctatcac 21 DNA PAT source 1..21 mol_type other DNA organism synthetic construct misc_feature 1..21 note reverse primer acaaggacctttgagcaacca 255 DNA PAT source 1..255 mol_type other DNA organism synthetic construct misc_feature 1..255 note DIR ORF atggttgcaactggcagtttgagcagcaagaacccggccagcatttcagaattgctggactgtggctatcacccagagagcctgctaagtggtgagtgtccccgctgtccacccttctcccctcccaaggtcggtgcctctggatctattgggtgggcgggctttcgacttcctcccctacctctatgtaggaggtgccacaaaatccatctgaggagactcctttccaaattttctaacatcttctctccatga 23 DNA PAT source 1..23 mol_type other DNA organism synthetic construct misc_feature 1..23 note sgRNA for DIR knock in atttcagagttgttggacggtgg 3000 DNA PAT source 1..3000 mol_type other DNA organism synthetic construct misc_feature 1..3000 note 5' homology arm caacttgactatatttggaatgaactacaatccagaattggaaggctcaccagtgacccttatctggaggcttggagatccttatctggatcttggtatggagatcttgagccatagtgactatggattccagaagattgaatctcagagtttaaggaacacacctttaacctgggctacacctttcatctgggattaaaggtgtggtggaacacacctttaatctgggctacaccttctgctggagacaatataaggacattggaagaagggagtctagctcttgctccttcgcctgcttgctgcgtgagactgagtaactgctagatccttggacttccattcacagcttctactgaacaattgttgggaattgggctgccgactgtaagtcatcaataaattcctttactatccataagttctgtgactctagagaaccctgactaatacagtagtcttggtttttcttgattctgtttgaatccttaaaatatttcattttcttaagtcatctacttagatgcaaagatagaagttggtgagagcatgtggcttgaaatcatgttaggtgacctttaagaaagacaatctctgtcggttactgcaggtatatgctctacacctcctgattcctcctgccctaagcttcctgatagcatcactcgttcagtcaaaaagaatgctcttatttgaaatttttgcatatgggtaaattgccttctgagataaaagcccagagagtgatataaaattgtagctaggtgagccaaaagtaaataccacattctagaactttctataactttgtcccggtggaagtatgaattatgcctaaatacccgagtggaaatgctgcattttctctattctacccacgtccattctgatcacttactatcataacactaataacattccttgaagcagggaggacgcagctgtcattaacaaggctgactagataaggaccagcggctctgtagcagctaaaggacccattctaaccggcttaaatgggattgcttgttttccacttttgttattaaaatagaagtggaaatctgctttccccagcctatcagcatcccgtgcctgagcctgcctgaccttgagcagtgcagctgcttcgggagagttaaaaatggaccttcaagctggctttagtttctgttgctctgcctgcaagtgtcctcccactgatctgtttatcacactctctttaccacttttgttgttgtttttccttctgagagtttttttagcctggacagcggatcctacttctcaaggccctaacctgcaggtccttccttgcacgtggggacccaaagtgtgcgggagtggtgctggaacctggccttgaatgaggttcaccctcaccccaagtatctcggtctcttccttttctgatccacctcaaccatctcagcaacctcagcaacctcgctgggaacgcccacccattgcaaaccctgtgccactggcgcagggcagcaggaagggtggcttagtaaagttagaagaaaacgtttggcatcgtgcttttagtggtgtcccagaccctctgttccctcttgctcaagactccccggaaagagtcgcagagcttatcctagcaggacaactctttattctcatgccccacaagctttttaaagaccactaaagcttaaacttcttcccaaacaagcccaccgggaacttttgcttctgggaatggcaattgctcctaactatcctgcggctggagcaggcaactgagatcctgttagtggttttgtttagtttcttggcactggccctcttcaattcaagttctgtgcctttcgaagccaggtcttagaacagggccaggctcgccctggccgcggagaaaaacggtttagtcacagatgtagtatccccaaagatgccccattgattcctgagtgcagctgaagctagagagcccttaccacatcccgaccacctgcggggagactgggtgcggactggagctgatgggttagagtcagaacatgggcataaaggatgctcacagagcacagtgtcaactaagccaaatacttattcagaatgacacagcaaagttttgggtcatgcctaacaggacctggagcttctagctgcttgaaaagttccatagccaaaacctgagcagtctgagcctggaagcaggcaagctgtgggcaggggctcacgccaaagaatctaggttagtggccgaagggagatgctctttaggagggtgaccagtggccaactcagggtgaccagtggccaactcaggaagcagcaggtgtccgcccaaggcgaggctgatcaccaactcccaggggcttgcctgggcccctgcctgggaaggaactgaaaggtgacctgaccctgcaggggcgggccggggcacgggcccgagcgagtggcgcaggtgggcgtgccggccaggaggagaggcgggacctataaagcgctctccattcgggaggcggagcggccgggtgcagcctgtgctggtgggcgcggtggcccgcagccttcggcttgctgcaggactgtgcaggggaccactgtccaagcttcggactactgaggggcgcctgcctcggtttacccttcagcgtctggtgaaatccggcaggtgagcgacccgaggaggcgggatagggcagggtcgcttcccagttgtgctgactttccagctacttccagcctgttggctttagttggcgcctccgctcctagggctttgtcaacttgagttgctaaggctggtccctccctttggaagggcagtgcccgggctagttcgccctggcgatgccgggactctgaggagcctgtgcacttcttgcgtgcttttgcaaacttctcactttttcctcccagcgcctagggaaagatccgttctgctccgcgagggaaacagagccgttgaccatggttgcaacgggcagtttgagcagtaagaacccggccagcatttcagagttgttggac 195 DNA PAT source 1..195 mol_type other DNA organism synthetic construct misc_feature 1..195 note Nucleic acid sequence encoding the last 64 amino acids of human QDLIR tgtggctatcacccagagagcctgctaagtggtgagtgtccccgctgtccacccttctcccctcccaaggtcggtgcctctggatctattgggtgggcgggctttcgacttcctcccctacctctatgtaggaggtgccacaaaatccatctgaggagactcctttccaaattttctaacatcttctctccatga 3000 DNA PAT source 1..3000 mol_type other DNA organism synthetic construct misc_feature 1..3000 note 3' homology arm ggtggctatcaccctgggagtctgctaagtggtgagtctctgggctgtccacctctttcctatccgaaggttgcggggcccctctctagtgtaagcgtccctgcctgagtgcgcgagacttcatctcctcctagagacacagaggactatgaaacccatctgagatctctttctaaacttcctttgcattgtctgcccaccaaaaacaaacgtaaggggaataatgcgttgttccagtctgctaagaggctcttggggatgaagttccctatctcatggttggaacattaagtgtacaggctgggtgtgtttaaagacatgcgcttacttgtgtagctagcaccaccacgtccgtcccccctccccccccccccccaaattctcccttttctgtttgccacctaatccttctctttcccgtctccagaagaccatccttcggcattttgttaccacgttagttttgcttttagaatgttaaataataagtgtatatgctcttctcagttcggtttgctttcccaccgtgtgatgttgtagagttatacacttacctcatcagtgagttccttttcgttgctaagtaagattctattatgaaatgcacccactgtgcattaatgtcagaagaaaacttgaacagccctggttctacaggaatcatcatttgcaacttgaaacgtcaatttctaatctgacagactattcaactatggaagagtggtgtgagcatatattaagtggcttaagctctttagattttcttcatctataagataatattataattctaaatttgtctgtcttcaatgtggataaaaatacagatatgatttggaatctatttgttggaatttaaaaaataaaacaaaaaacaaaaaacaagcagtgccctctaagtgagctaatgacatagaaaaccaggagaggagaggaaggctcgtggccgcagtgtggcatggcacccggccaagtagtaggattaatttggaaggtaggtgaccaaatgcagagctatcttttttcctctgagtaaacctaactttgttttcctgggactgttttgttttgctcagctaagaaaaactgacccagcccggttataaataaactcaggaacctcaaaagtcctctctcctctgcgagcaaacttgaatcaaacaggtcaaagccaacactggacctccaggccatcctagtaaggacttctaacgaaaactaaggctggtagatcattagcattgggacaggctcactttacagcgtttgaaaccattctgcacgccaacggtccctttgttaagcaatagtgatgatttttattccagatgttctgcatggacttgtgaaaaaaaactaaaataaacttggaaaaccctacagagagagcaagggggaaaggagatcactagtaatcttggatgaccttggacagatttttgccacataatcctcaagactacggggagagaagaaattgtaaaagagggttgttttaacctattcattatgtttatcagagtctttagggcatagatgagctttcagtcctgaaaggttagaaggcctgttctattacctacatggttaacagcaaaattgagatgagagaaagcagtattaaaagcaggtagatatgaagctagaagtggccagtcttactatgccagtaagacctcgtacaccttgcttctctgttcactaaaactttcatttctttcagatttcgactactgggattatgttgtccctgagcccaacctcaacgaggtggtatttgaagagacaacatgccagaatttggttaaaatgttggagaactgtctgtccagatcaaagcaaaccaaacttggttgctctaaggtcttggtccctgagaaactgacccagagaattgctcaagatgtcctgcggctctcgtccacggagccctgcggccttcggggttgtgttatgcacgtgaacttggagattgaaaatgtatgtaaaaagctggataggatcgtgtgtgatgctagcgtggtgccgacctttgagctcacgcttgtgtttaagcaggagagctgcccgtggaccagcctcaaggacttcttctttagcagaggtcgcttctcctcaggccttaagcgaactctgattctcagctcaggatttcgacttgtgaagaaaaaactgtactccctgattggaacgacagtcattgaagagtgctaaggaggaaaaacaattaaaggcccctaatgagtggataataaacctctgaagctatccagccagtcatttgtagtttgcctgcttgccctcaccaggaaatcccagatttaggcccttttccttctgtgtgtctcacccatagcaacccactttaaaggctgattttttttttttttgtccagcttttaagacataattcaagagagcaaattgcccacatatgtgtccatatgggatagaatggaataaggaggaaaaagaagcaatctcctttaattttatatttggtctcatatttagcaggtagtcttagcaacagagcaagatccactttgcctaatttgactttcatgtgcccagggcttgctgttattgtacccgcctctctctctctctctctctctctctctgtgtgtgtgtgtgtgtgtgtgtgtatatatatatatctgtctgtctctctctgtctctctgtctctctctctctcctcccctccctccctctctgagagccatcttttcaatgacaggacctgacaagatgatgtgcagccccatcaaaacatatagagatctgcactaccagcttcctccctgaatttgaagagcttttacacaagagggtacagttaaattgatccgctggattaaaacatcaccttacatagagcgaataaacatcacagtgacaagagcatggcttccctgcctggtggtctccccacacccgtgcccaggagacacgagaagtgtgcctcttactgcaatgattacatgatggctttcagt 20 DNA PAT source 1..20 mol_type other DNA organism synthetic construct misc_feature 1..20 note Primer P1 gaggagcctgtgcacttctt 20 DNA PAT source 1..20 mol_type other DNA organism synthetic construct misc_feature 1..20 note P2 primer cacacccagcctgtacactt 18 DNA PAT source 1..18 mol_type other DNA organism synthetic construct misc_feature 1..18 note P3 primer tgcctcggtttacccttc 23 DNA PAT source 1..23 mol_type other DNA organism synthetic construct misc_feature 1..23 note P4 primer ccagctttttacatacattttca 24 DNA PAT source 1..24 mol_type other DNA organism synthetic construct misc_feature 1..24 note P5 primer aagatgttagaaaatttggaaagg 60 DNA PAT source 1..60 mol_type other DNA organism synthetic construct misc_feature 1..60 note QD202 DNA atgtacggcaggaagaagaggaggcagaggaggaggagccccgtggacgtggtgtgcagc