WO PO25-5304 WO PCT/KR2023/008718 2023-06-22 SL BIGEN, Inc. Novel fusion protein and use thereof 61 31 AA PAT REGION 1..31 note human GLP-1 source 1..31 mol_type protein organism synthetic construct HGEGTFTSDVSSYLEGQAAKEFIAWLVKGRG 31 AA PAT REGION 1..31 note human GLP-1 source 1..31 mol_type protein organism synthetic construct HAEGTFTSDVSSYLEGQAAKEFIAWLVKGRG 39 AA PAT REGION 1..39 note Exendin 3 source 1..39 mol_type protein organism synthetic construct HSDGTFTSDLSKQMEEEAVRLFIEWLKNGGPSSGAPPPS 39 AA PAT REGION 1..39 note Exendin 4 source 1..39 mol_type protein organism synthetic construct HGEGTFTSDLSKQMEEEAVRLFIEWLKNGGPSSGAPPPS 39 AA PAT REGION 1..39 note GLP-1/Exendin 4 hybrid source 1..39 mol_type protein organism synthetic construct HGEGTFTSDVSSYLEGQAAKEFIAWLVKGGPSSGAPPPS 44 AA PAT REGION 1..44 note Lixisenatide source 1..44 mol_type protein organism synthetic construct HGEGTFTSDLSKQMEEEAVRLFIEWLKNGGPSSGAPPSKKKKKK 905 AA PAT REGION 1..905 note Exendin 4-XTEN source 1..905 mol_type protein organism synthetic construct HGEGTFTSDLSKQMEEEAVRLFIEWLKNGGPSSGAPPPSGGSPAGSPTSTEEGTSESATPESGPGTSTEPSEGSAPGSPAGSPTSTEEGTSTEPSEGSAPGTSTEPSEGSAPGTSESATPESGPGSEPATSGSETPGSEPATSGSETPGSPAGSPTSTEEGTSESATPESGPGTSTEPSEGSAPGTSTEPSEGSAPGSPAGSPTSTEEGTSTEPSEGSAPGTSTEPSEGSAPGTSESATPESGPGTSTEPSEGSAPGTSESATPESGPGSEPATSGSETPGTSTEPSEGSAPGTSTEPSEGSAPGTSESATPESGPGTSESATPESGPGSPAGSPTSTEEGTSESATPESGPGSEPATSGSETPGTSESATPESGPGTSTEPSEGSAPGTSTEPSEGSAPGTSTEPSEGSAPGTSTEPSEGSAPGTSTEPSEGSAPGTSTEPSEGSAPGSPAGSPTSTEEGTSTEPSEGSAPGTSESATPESGPGSEPATSGSETPGTSESATPESGPGSEPATSGSETPGTSESATPESGPGTSTEPSEGSAPGTSESATPESGPGSPAGSPTSTEEGSPAGSPTSTEEGSPAGSPTSTEEGTSESATPESGPGTSTEPSEGSAPGTSESATPESGPGSEPATSGSETPGTSESATPESGPGSEPATSGSETPGTSESATPESGPGTSTEPSEGSAPGSPAGSPTSTEEGTSESATPESGPGSEPATSGSETPGTSESATPESGPGSPAGSPTSTEEGSPAGSPTSTEEGTSTEPSEGSAPGTSESATPESGPGTSESATPESGPGTSESATPESGPGSEPATSGSETPGSEPATSGSETPGSPAGSPTSTEEGTSTEPSEGSAPGTSTEPSEGSAPGSEPATSGSETPGTSESATPESGPGTSTEPSEGSAPG 645 AA PAT REGION 1..645 note Albiglutide source 1..645 mol_type protein organism synthetic construct HGEGTFTSDVSSYLEGQAAKEFIAWLVKGRHGEGTFTSDVSSYLEGQAAKEFIAWLVKGRDAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL 31 AA PAT REGION 1..31 note Liraglutide source 1..31 mol_type protein organism synthetic construct HAEGTFTSDVSSYLEGQAAKEFIAWLVRGRG 30 AA PAT REGION 1..30 note Taspoglutide MOD_RES 2 note METHYLATION, MOD_RES 29 note METHYLATION, source 1..30 mol_type protein organism synthetic construct HXEGTFTSDVSSYLEGQAAKEFIAWLVKXR 108 AA PAT REGION 1..108 note GLP-1 tandem repeat source 1..108 mol_type protein organism synthetic construct HGEGTFTSDVSSYLEGQAAKEFIAWLVKGGPSSGAPPPSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSHGEGTFTSDVSSYLEGQAAKEFIAWLVKGGPSSGAPPPS 215 AA PAT REGION 1..215 note hybrid Fc region source 1..215 mol_type protein organism synthetic construct SHTQPLGVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSLGK 215 AA PAT REGION 1..215 note hybrid Fc hole source 1..215 mol_type protein organism synthetic construct SHTQPLGVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVCTLPPSQEEMTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSLGK 215 AA PAT REGION 1..215 note hybrid Fc knob source 1..215 mol_type protein organism synthetic construct SHTQPLGVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPCQEEMTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSLGK 215 AA PAT REGION 1..215 note hybrid Fc knob source 1..215 mol_type protein organism synthetic construct SHTQPLGVFLFPPKPKDQLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPCQEEMTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVLHEALHNHYTQKSLSLSLGK 215 AA PAT REGION 1..215 note hybrid Fc hole source 1..215 mol_type protein organism synthetic construct SHTQPLGVFLFPPKPKDQLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVCTLPPSQEEMTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSRLTVDKSRWQEGNVFSCSVLHEALHNHYTQKSLSLSLGK 33 AA PAT REGION 1..33 note human GLP-2 mutant (A2G) source 1..33 mol_type protein organism synthetic construct HGDGSFSDEMNTILDNLAARDFINWLIQTKITD 33 AA PAT REGION 1..33 note human GLP-2 wild type source 1..33 mol_type protein organism synthetic construct HADGSFSDEMNTILDNLAARDFINWLIQTKITD 33 AA PAT REGION 1..33 note human GLP-2 mutant (A2G, N16G, L17Q) source 1..33 mol_type protein organism synthetic construct HGDGSFSDEMNTILDGQAARDFINWLIQTKITD 33 AA PAT REGION 1..33 note human GLP-2 mutant (A2G, L17Q) source 1..33 mol_type protein organism synthetic construct HGDGSFSDEMNTILDNQAARDFINWLIQTKITD 39 AA PAT REGION 1..39 note Glepaglutide source 1..39 mol_type protein organism synthetic construct HGEGTFSSELATILDALAARDFIAWLIATKITDKKKKKK 42 AA PAT REGION 1..42 note GLP-2 analogue 10 MOD_RES 11 note linked with a lipidated cross-linker via thiol groups of 11th and18th amino acids, cystein MOD_RES 18 note linked with a lipidated cross-linker via thiol groups of 11th and18th amino acids, cystein source 1..42 mol_type protein organism synthetic construct HGDGSFSDEMCTILDNLCARDFINWLIQTKITDPSSGAPPPS 287 AA PAT REGION 1..287 note first fusion protein of bispecific fusion protein containingGLP-1 analogue source 1..287 mol_type protein organism synthetic construct HGEGTFTSDVSSYLEGQAAKEFIAWLVKGGPSSGAPPPSGGGGSGGGGSGGGGSEKEKEEQEERTHTCPPCPSHTQPLGVFLFPPKPKDQLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPCQEEMTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVLHEALHNHYTQKSLSLSLGK 309 AA PAT REGION 1..309 note first fusion protein of bispecific fusion protein containingGLP-1 analogue source 1..309 mol_type protein organism synthetic construct HGEGTFTSDVSSYLEGQAAKEFIAWLVKGGPSSGAPPPSGGGGSGGGGSGGGGSAKNTTAPATTRNTTRGGEEKKKEKEKEEQEERTHTCPPCPSHTQPLGVFLFPPKPKDQLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPCQEEMTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVLHEALHNHYTQKSLSLSLGK 353 AA PAT REGION 1..353 note first fusion protein of bispecific fusion protein containingGLP-1 analogue source 1..353 mol_type protein organism synthetic construct HGEGTFTSDVSSYLEGQAAKEFIAWLVKGGPSSGAPPPSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSHGEGTFTSDVSSYLEGQAAKEFIAWLVKGGPSSGAPPPSGGGGSGGGGSGGGGSEPKSSDKTHTCPPCPSHTQPLGVFLFPPKPKDQLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPCQEEMTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVLHEALHNHYTQKSLSLSLGK 281 AA PAT REGION 1..281 note second fusion protein of bispecific fusion protein containingGLP-2 analogue source 1..281 mol_type protein organism synthetic construct HGDGSFSDEMNTILDNLAARDFINWLIQTKITDGGGGSGGGGSGGGGSEKEKEEQEERTHTCPPCPSHTQPLGVFLFPPKPKDQLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVCTLPPSQEEMTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSRLTVDKSRWQEGNVFSCSVLHEALHNHYTQKSLSLSLGK 304 AA PAT REGION 1..304 note second fusion protein of bispecific fusion protein containingGLP-2 analogue source 1..304 mol_type protein organism synthetic construct HGDGSFSDEMNTILDNLAARDFINWLIQTKITDGGGGGSGGGGSGGGGSAKNTTAPATTRNTTRGGEEKKKEKEKEEQEERTHTCPPCPSHTQPLGVFLFPPKPKDQLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVCTLPPSQEEMTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSRLTVDKSRWQEGNVFSCSVLHEALHNHYTQKSLSLSLGK 278 AA PAT REGION 1..278 note second fusion protein of bispecific fusion protein containingGLP-2 analogue source 1..278 mol_type protein organism synthetic construct HGDGSFSDEMNTILDGQAARDFINWLIQTKITDGGGGSGGGGSGGGGSEPKSSDKTHTCPPCPSHTQPLGVFLFPPKPKDQLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVCTLPPSQEEMTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSRLTVDKSRWQEGNVFSCSVLHEALHNHYTQKSLSLSLGK 15 AA PAT REGION 1..15 note linker peptide source 1..15 mol_type protein organism synthetic construct EPKSSDKTHTCPPCP 15 AA PAT REGION 1..15 note linker peptide source 1..15 mol_type protein organism synthetic construct EPKSCDKTHTCPPCP 30 AA PAT REGION 1..30 note linker peptide source 1..30 mol_type protein organism synthetic construct GGGGSGGGGSGGGGSEPKSSDKTHTCPPCP 30 AA PAT REGION 1..30 note linker peptide source 1..30 mol_type protein organism synthetic construct GGGGSGGGGSGGGGSEPKSCDKTHTCPPCP 40 AA PAT REGION 1..40 note linker peptide source 1..40 mol_type protein organism synthetic construct AKATTAPATTRNTGRGGEEKKKEKEKEEQEERETKTPECP 33 AA PAT REGION 1..33 note linker peptide source 1..33 mol_type protein organism synthetic construct GGGGSGGGGSGGGGSEKEKEEQEERTHTCPPCP 55 AA PAT REGION 1..55 note linker peptide source 1..55 mol_type protein organism synthetic construct GGGGSGGGGSGGGGSAKNTTAPATTRNTTRGGEEKKKEKEKEEQEERTHTCPPCP 20 AA PAT REGION 1..20 note linker peptide source 1..20 mol_type protein organism synthetic construct AAGSGGGGGSGGGGSGGGGS 15 AA PAT REGION 1..15 note linker peptide source 1..15 mol_type protein organism synthetic construct GGGGSGGGGSGGGGS 5 AA PAT REGION 1..5 note linker peptide source 1..5 mol_type protein organism synthetic construct GGSGG 9 AA PAT REGION 1..9 note linker peptide source 1..9 mol_type protein organism synthetic construct GGSGGSGGS 6 AA PAT REGION 1..6 note linker peptide source 1..6 mol_type protein organism synthetic construct GGGSGG 5 AA PAT REGION 1..5 note linker peptide unit sequence source 1..5 mol_type protein organism synthetic construct GGGGS 6 AA PAT REGION 1..6 note linker peptide unit sequence source 1..6 mol_type protein organism synthetic construct GSSGGS 18 AA PAT REGION 1..18 note linker peptide source 1..18 mol_type protein organism synthetic construct KESGSVSSEQLAQFRSLD 14 AA PAT REGION 1..14 note linker peptide source 1..14 mol_type protein organism synthetic construct EGKSSGSGSESKST 12 AA PAT REGION 1..12 note linker peptide source 1..12 mol_type protein organism synthetic construct GSAGSAAGSGEF 5 AA PAT REGION 1..5 note linker peptide unit sequence source 1..5 mol_type protein organism synthetic construct EAAAK 12 AA PAT REGION 1..12 note linker peptide source 1..12 mol_type protein organism synthetic construct CRRRRRREAEAC 46 AA PAT REGION 1..46 note linker peptide source 1..46 mol_type protein organism synthetic construct AEAAAKEAAAKEAAAKEAAAKALEAEAAAKEAAAKEAAAKEAAAKA 8 AA PAT REGION 1..8 note linker peptide source 1..8 mol_type protein organism synthetic construct GGGGGGGG 6 AA PAT REGION 1..6 note linker peptide source 1..6 mol_type protein organism synthetic construct GGGGGG 13 AA PAT REGION 1..13 note linker peptide source 1..13 mol_type protein organism synthetic construct AEAAAKEAAAAKA 5 AA PAT REGION 1..5 note linker peptide source 1..5 mol_type protein organism synthetic construct PAPAP 17 AA PAT REGION 1..17 note linker peptide source 1..17 mol_type protein organism synthetic construct VSQTSKLTRAETVFPDV 6 AA PAT REGION 1..6 note linker peptide source 1..6 mol_type protein organism synthetic construct PLGLWA 10 AA PAT REGION 1..10 note linker peptide source 1..10 mol_type protein organism synthetic construct TRHRQPRGWE 10 AA PAT REGION 1..10 note linker peptide source 1..10 mol_type protein organism synthetic construct AGNRVRRSVG 8 AA PAT REGION 1..8 note linker peptide source 1..8 mol_type protein organism synthetic construct RRRRRRRR 4 AA PAT REGION 1..4 note linker peptide source 1..4 mol_type protein organism synthetic construct GFLG 31 AA PAT REGION 1..31 note linker peptide source 1..31 mol_type protein organism synthetic construct GSSGGSGSSGGSGGGDEADGSRGSQKAGVDE 15 AA PAT REGION 1..15 note linker peptide source 1..15 mol_type protein organism synthetic construct GSTSGSGKPGSGEGS 103 AA PAT REGION 1..103 note IgG1 CH3 domain source 1..103 mol_type protein organism synthetic construct EPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK