JP FP24-0190-00 WO PCT/JP2023/015233 2023-04-14 Chugai Pharmaceutical Co., Ltd. METHOD FOR SCREENING TARGET MOLECULE CAPABLE OF FORMING COMPLEX WITH PLURAL KINDS OF TARGET MOLECULES AND METHOD FOR PRODUCING TARGET MOLECULE INCLUDING THE METHOD FOR SCREENING 67 149 AA PAT source 1..149 mol_type protein organism synthetic construct SITE 144 note Biotinylation MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLEGGGSSDYKDDDDKSSGENLYFQSSGGGLNDIFEAQKIEWHE 142 AA PAT source 1..142 mol_type protein organism synthetic construct SITE 137 note Biotinylation MELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQGGGSSDYKDDDDKSSGENLYFQSSGGGLNDIFEAQKIEWHE 141 AA PAT source 1..141 mol_type protein organism synthetic construct MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLEGGGSSDYKDDDDKGGSSLPMTGGGGSSHHHHHH 134 AA PAT source 1..134 mol_type protein organism synthetic construct MELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQGGGSSDYKDDDDKGGSSLPMTGGGGSSHHHHHH 280 AA PAT source 1..280 mol_type protein organism synthetic construct SITE 11 note Biotinylation MGLNDIFEAQKIEWHESSGENLYFQSGGGSSDYKDDDDKSGGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLEGSGSSGLEVLFQGPSSGSSGTELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQGGGSSHHHHHH 206 AA PAT source 1..206 mol_type protein organism synthetic construct SITE 201 note Biotinylation GPMVNPTVFFDIAVDGEPLGRVSFELFADKVPKTAENFRALSTGEKGFGYKGSCFHRIIPGFMCQGGDFTRHNGTGGKSIYGEKFEDENFILKHTGPGILSMANAGPNTNGSQFFICTAKTEWLDGKHVVFGKVKEGMNIVEAMERFGSRNGKTSKKITIADCGQLEGSSDYKDDDDKSSGENLYFQSSGGGLNDIFEAQKIEWHE 738 AA PAT source 1..738 mol_type protein organism synthetic construct SITE 733 note Biotinylation GPMGNEASYPLEMCSHFDADEIKRLGKRFKKLDLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIINADKDGDGRISFEEFCAVVGGLDIHKKMVVDVGGGGGGMSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKIRAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSINKALTSETNGTDSNGSNSSNIQGSSDYKDDDDKSSGENLYFQSSGGGLNDIFEAQKIEWHE 196 AA PAT source 1..196 mol_type protein organism synthetic construct MVNPTVFFDIAVDGEPLGRVSFELFADKVPKTAENFRALSTGEKGFGYKGSCFHRIIPGFMCQGGDFTRHNGTGGKSIYGEKFEDENFILKHTGPGILSMANAGPNTNGSQFFICTAKTEWLDGKHVVFGKVKEGMNIVEAMERFGSRNGKTSKKITIADCGQLEGSSDYKDDDDKGGSSLPMTGGGGSSHHHHHH 728 AA PAT source 1..728 mol_type protein organism synthetic construct MGNEASYPLEMCSHFDADEIKRLGKRFKKLDLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIINADKDGDGRISFEEFCAVVGGLDIHKKMVVDVGGGGGGMSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKIRAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSINKALTSETNGTDSNGSNSSNIQGSSDYKDDDDKGGSSLPMTGGGGSSHHHHHH 920 AA PAT source 1..920 mol_type protein organism synthetic construct SITE 915 note Biotinylation MHHHHHHGNEASYPLEMCSHFDADEIKRLGKRFKKLDLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIINADKDGDGRISFEEFCAVVGGLDIHKKMVVDVGGGGGGSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAARAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSEEAKGLDRINERMPPRRDAMPSDANLNSINKALTSETNGTDSNGSNSSNIDGGGGSGLEVLFQGPGGGSGGGGSGGGSTVNPTVFFDIAVDGEPLGRVSFELFADKVPKTAENFRALSTGEKGFGYKGSCFHRIIPGFMCQGGDFTRHNGTGGKSIYGEKFEDENFILKHTGPGILSMANAGPNTNGSQFFICTAKTEWLDGKHVVFGKVKEGMNIVEAMERFGSRNGKTSKKITIADCGQLEDYKDDDDKGSGGENLYFQSSGGGLNDIFEAQKIEWHE 107 DNA PAT source 1..107 mol_type other DNA organism synthetic construct misc_feature 50..76 note 9 repeats of the triplet randomly selected from the group consisting of TTT, TTG, CTT, CTG, ATT, ATG, GTT, GTG, TCT, TCG, CCG, ACT, GCT, TAC, CAT, CAG, AAC, GAT, GAG, TGC, TGG, CGT, CGG, AGT, AGG, and GGT. gtaatacgactcactatagggttaactttaataaggagatataaatatgnnnnnnnnnnnnnnnnnnnnnnnnnnntagccgaccggcaccggcaccggcaaaaaaa 104 DNA PAT source 1..104 mol_type other DNA organism synthetic construct misc_feature 50..73 note 8 repeats of the triplet randomly selected from the group consisting of TTT, TTG, CTT, CTG, ATT, ATG, GTT, GTG, TCT, TCG, CCG, ACT, GCT, TAC, CAT, CAG, AAC, GAT, GAG, TGC, TGG, CGT, CGG, AGT, AGG, and GGT. gtaatacgactcactatagggttaactttaataaggagatataaatatgnnnnnnnnnnnnnnnnnnnnnnnntagccgaccggcaccggcaccggcaaaaaaa 38 DNA PAT source 1..38 mol_type other DNA organism synthetic construct gtaatacgactcactatagggttaactttaataaggag 31 DNA PAT source 1..31 mol_type other DNA organism synthetic construct tttttttgccggtgccggtgccggtcggcta 110 DNA PAT source 1..110 mol_type other DNA organism synthetic construct ggcgtaatacgactcactatagggttaactttaataaggagatataaatatgcattcgggtgagtgccatactagttcttagccgaccggcaccggcaccggcaaaaaaa 110 DNA PAT source 1..110 mol_type other DNA organism synthetic construct ggcgtaatacgactcactatagggttaactttaataaggagatataaatatgttttcggagtgcactaaccagccgttgtagccgaccggcaccggcaccggcaaaaaaa 110 DNA PAT source 1..110 mol_type other DNA organism synthetic construct ggcgtaatacgactcactatagggttaactttaataaggagatataaatatgtctggtcgtcatctgtgccatattaggtagccgaccggcaccggcaccggcaaaaaaa 110 DNA PAT source 1..110 mol_type other DNA organism synthetic construct ggcgtaatacgactcactatagggttaactttaataaggagatataaatatgtgggttttgctgtgctctactggtggttagccgaccggcaccggcaccggcaaaaaaa 89 RNA PAT source 1..89 mol_type other RNA organism synthetic construct gggttaactttaataaggagatataaatatgcattcgggtgagtgccatactagttcttagccgaccggcaccggcaccggcaaaaaaa 89 RNA PAT source 1..89 mol_type other RNA organism synthetic construct gggttaactttaataaggagatataaatatgttttcggagtgcactaaccagccgttgtagccgaccggcaccggcaccggcaaaaaaa 89 RNA PAT source 1..89 mol_type other RNA organism synthetic construct gggttaactttaataaggagatataaatatgtctggtcgtcatctgtgccatattaggtagccgaccggcaccggcaccggcaaaaaaa 89 RNA PAT source 1..89 mol_type other RNA organism synthetic construct gggttaactttaataaggagatataaatatgtgggttttgctgtgctctactggtggttagccgaccggcaccggcaccggcaaaaaaa 110 DNA PAT source 1..110 mol_type other DNA organism synthetic construct ggcgtaatacgactcactatagggttaactttaataaggagatataaatatgcgtcagctgattcggtgggttgtgacttagccgaccggcaccggcaccggcaaaaaaa 110 DNA PAT source 1..110 mol_type other DNA organism synthetic construct ggcgtaatacgactcactatagggttaactttaataaggagatataaatatgtggtttgataactcgaacgtggtgacttagccgaccggcaccggcaccggcaaaaaaa 110 DNA PAT source 1..110 mol_type other DNA organism synthetic construct ggcgtaatacgactcactatagggttaactttaataaggagatataaatatgatgccgaggaacgttcttactactcagtagccgaccggcaccggcaccggcaaaaaaa 110 DNA PAT source 1..110 mol_type other DNA organism synthetic construct ggcgtaatacgactcactatagggttaactttaataaggagatataaatatgtttagtggtattccgtgggttgtgatttagccgaccggcaccggcaccggcaaaaaaa 89 RNA PAT source 1..89 mol_type other RNA organism synthetic construct gggttaactttaataaggagatataaatatgcgtcagctgattcggtgggttgtgacttagccgaccggcaccggcaccggcaaaaaaa 89 RNA PAT source 1..89 mol_type other RNA organism synthetic construct gggttaactttaataaggagatataaatatgtggtttgataactcgaacgtggtgacttagccgaccggcaccggcaccggcaaaaaaa 89 RNA PAT source 1..89 mol_type other RNA organism synthetic construct gggttaactttaataaggagatataaatatgatgccgaggaacgttcttactactcagtagccgaccggcaccggcaccggcaaaaaaa 89 RNA PAT source 1..89 mol_type other RNA organism synthetic construct gggttaactttaataaggagatataaatatgtttagtggtattccgtgggttgtgatttagccgaccggcaccggcaccggcaaaaaaa 28 DNA PAT source 1..28 mol_type other DNA organism synthetic construct misc_feature 1 note Substituent having an azide group is bound to phosphoric acid group. tttttttgccggtgccggtgccggtcgg 110 DNA PAT source 1..110 mol_type other DNA organism synthetic construct ggcgtaatacgactcactatagggttaactttaataaggagatataaatatggtttgctcttctaggtctctgaaccggtagccgaccggcaccggcaccggcaaaaaaa 110 DNA PAT source 1..110 mol_type other DNA organism synthetic construct ggcgtaatacgactcactatagggttaactttaataaggagatataaatatgactctgaacgagtgcggtcattgccattagccgaccggcaccggcaccggcaaaaaaa 110 DNA PAT source 1..110 mol_type other DNA organism synthetic construct ggcgtaatacgactcactatagggttaactttaataaggagatataaatatgcagttggagtggtttgtttggccgacttagccgaccggcaccggcaccggcaaaaaaa 89 RNA PAT source 1..89 mol_type other RNA organism synthetic construct gggttaactttaataaggagatataaatatggtttgctcttctaggtctctgaaccggtagccgaccggcaccggcaccggcaaaaaaa 89 RNA PAT source 1..89 mol_type other RNA organism synthetic construct gggttaactttaataaggagatataaatatgactctgaacgagtgcggtcattgccattagccgaccggcaccggcaccggcaaaaaaa 89 RNA PAT source 1..89 mol_type other RNA organism synthetic construct gggttaactttaataaggagatataaatatgcagttggagtggtttgtttggccgacttagccgaccggcaccggcaccggcaaaaaaa 28 DNA PAT source 1..28 mol_type other DNA organism synthetic construct tttttttgccggtgccggtgccggtcgg 110 DNA PAT source 1..110 mol_type other DNA organism synthetic construct ggcgtaatacgactcactatagggttaactttaataaggagatataaatatgtctactgctgtgactaggttgaacagttagccgaccggcaccggcaccggcaaaaaaa 110 DNA PAT source 1..110 mol_type other DNA organism synthetic construct ggcgtaatacgactcactatagggttaactttaataaggagatataaatatggattggccgggtttgcggactattggttagccgaccggcaccggcaccggcaaaaaaa 110 DNA PAT source 1..110 mol_type other DNA organism synthetic construct ggcgtaatacgactcactatagggttaactttaataaggagatataaatatgatttgggttcttactaacactgttgtttagccgaccggcaccggcaccggcaaaaaaa 89 RNA PAT source 1..89 mol_type other RNA organism synthetic construct gggttaactttaataaggagatataaatatgtctactgctgtgactaggttgaacagttagccgaccggcaccggcaccggcaaaaaaa 89 RNA PAT source 1..89 mol_type other RNA organism synthetic construct gggttaactttaataaggagatataaatatggattggccgggtttgcggactattggttagccgaccggcaccggcaccggcaaaaaaa 89 RNA PAT source 1..89 mol_type other RNA organism synthetic construct gggttaactttaataaggagatataaatatgatttgggttcttactaacactgttgtttagccgaccggcaccggcaccggcaaaaaaa 9 AA PAT source 1..9 mol_type protein organism synthetic construct PTGTGTGKK 160 AA PAT source 1..160 mol_type protein organism synthetic construct SITE 155 note Biotinylation GLGGGGSGGGGSGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLEGGGSSDYKDDDDKSSGENLYFQSSGGGLNDIFEAQKIEWHE 328 AA PAT source 1..328 mol_type protein organism synthetic construct ARDGDYLHLPSEVSRFFRPQETNDDHGEDSVGTRWAVLIAGSKGYANYRHQAGVCHAYQILKRGGLKDENIVVFMYDDIAYNESNPRPGVIINSPHGSDVYAGVPKDYTGEEVNAKNFLAAILGNKSAITGGSGKVVDSGPNDHIFIYYTDHGAAGVIGMPSKPYLYADELNDALKKKHASGTYKSLVFYLEACESGSMFEGILPEDLNIYALTSTNTTESSWAYYCPAQENPPPPEYNVCLGDLFSVAWLEDSDVQNSWYETLNQQYHHVDKRISHASHATQYGNLKLGEEGLFVYMGSNPANDNYTSLDGNALTPSSIVVNQRDAD 156 AA PAT source 1..156 mol_type protein organism synthetic construct MQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATREQLNRGVSFAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRNVKPTAVEVLDEQKGKDKQLTLITCDDYNEETGVWETRKIFVATEVKLEHHHHHH 727 AA PAT source 1..727 mol_type protein organism synthetic construct SITE 727 note N-{4-(4-methyltetrazinyl(phenlyacetyl)}lysine MGNEASYPLEMCSHFDADEIKRLGKRFKKLDLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIINADKDGDGRISFEEFCAVVGGLDIHKKMVVDVGGGGGGMSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKIRAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSINKALTSETNGTDSNGSNSSNIQGSSDYKDDDDKGGSSLPMTGGGGSGGGGSK 174 AA PAT source 1..174 mol_type protein organism synthetic construct SITE 1 note N-{(E)-cyclooct-4-enoxycarbonyl}glycine SITE 169 note biotinylation GGSGGGGSGGGGSNGLGGGGSGGGGSGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLEGGGSSDYKDDDDKSSGENLYFQSSGGGLNDIFEAQKIEWHE 107 DNA PAT source 1..107 mol_type other DNA organism synthetic construct gtaatacgactcactatagggttaactttaataaggagatataaatatgtgcacttctactggttactggcttcattagccgaccggcaccggcaccggcaaaaaaa 107 DNA PAT source 1..107 mol_type other DNA organism synthetic construct gtaatacgactcactatagggttaactttaataaggagatataaatatgattaacgtgttttcgggttgctacccgtagccgaccggcaccggcaccggcaaaaaaa 89 RNA PAT source 1..89 mol_type other RNA organism synthetic construct gggttaactttaataaggagatataaatatgtgcacttctactggttactggcttcattagccgaccggcaccggcaccggcaaaaaaa 89 RNA PAT source 1..89 mol_type other RNA organism synthetic construct gggttaactttaataaggagatataaatatgattaacgtgttttcgggttgctacccgtagccgaccggcaccggcaccggcaaaaaaa 311 AA PAT source 1..311 mol_type protein organism synthetic construct SITE 306 note Biotinylation MHHHHHHSGGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLEGSGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGTELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQGGGSSDYKDDDDKSSGENLYFQSSGGGLNDIFEAQKIEWHE 289 AA PAT source 1..289 mol_type protein organism synthetic construct SITE 11 note Biotinylation MGLNDIFEAQKIEWHESSGENLYFQSGGGSSDYKDDDDKSGGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLEGSGSSGSSGLEVLFQGPSSGSSGSSGSSGTELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQGGGSSHHHHHH 170 AA PAT source 1..170 mol_type protein organism synthetic construct SITE 23 note Biotinylation MDYKDDDDKGGSSGLNDIFEAQKIEWHESSGENLYFQGGGGSGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLEGGGSSLPMTGGGGSSHHHHHH 143 AA PAT source 1..143 mol_type protein organism synthetic construct SITE 11 note Biotinylation MGLNDIFEAQKIEWHESSGENLYFQGGGGSELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQGGGSSDYKDDDDK 276 AA PAT source 1..276 mol_type protein organism synthetic construct SITE 23 note Biotinylation MDYKDDDDKGGSSGLNDIFEAQKIEWHESSGENLYFQGGGGSGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLEGGGSSLPMTGGGGSELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQGGGSSDYKDDDDK 125 AA PAT source 1..125 mol_type protein organism synthetic construct GLGGGGSGGGGSELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQGGGSSDYKDDDDK 307 AA PAT source 1..307 mol_type protein organism synthetic construct SITE 23 note Biotinylation MDYKDDDDKGGSSGLNDIFEAQKIEWHESSGENLYFQGGGGSGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLEGGGSSLPMTGGGGGGGSGGGGSGGGGSGGGGSNGLGGGGSGGGGSELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQGGGSSDYKDDDDK 27 AA PAT source 1..27 mol_type protein organism unidentified GGGGGGGSGGGGSGGGGSGGGGSNGLH 306 AA PAT source 1..306 mol_type protein organism synthetic construct SITE 301 note Biotinylation MELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQGGGSSDYKDDDDKGGSSLPMTGGGGGGGSGGGGSGGGGSGGGGSNGLGGGGSGGGGSGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLEGGGSSDYKDDDDKSSGENLYFQSSGGGLNDIFEAQKIEWHE 169 AA PAT source 1..169 mol_type protein organism synthetic construct SITE 169 note N-{4-(4-methyltetrazinyl(phenlyacetyl)}lysine SITE 23 note Biotinylation MDYKDDDDKGGSSGLNDIFEAQKIEWHESSGENLYFQGGGGSGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLEGGGSSLPMTGGGGSGGGGSK 138 AA PAT source 1..138 mol_type protein organism synthetic construct SITE 138 note N-{(E)-cyclooct-4-enoxycarbonyl}lycine MELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQGGGSSDYKDDDDKGGSSLPMTGGGGSGGGGSGGGGSK 174 AA PAT source 1..174 mol_type protein organism synthetic construct SITE 1 note N-{4-(4-methyltetrazinyl(phenlyacetyl)}glysine SITE 169 note Biotinylation GGSGGGGSGGGGSNGLGGGGSGGGGSGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLEGGGSSDYKDDDDKSSGENLYFQSSGGGLNDIFEAQKIEWHE 139 AA PAT source 1..139 mol_type protein organism synthetic construct SITE 1 note N-{(E)-cyclooct-4-enoxycarbonyl}glycine GGSGGGGSGGGGSNGLGGGGSGGGGSELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQGGGSSDYKDDDDK