OPP20222190KR KR 10-2021-0098072 2021-07-26 CJ CheilJedang Corporation CJ CheilJedang Corporation Microorganism with weakened activity of LacI family DNA-binding transcriptional regulator and production method of L-glutamic acid using the same 24 309 AA PAT REGION 1..309 note snythetic_LacI family DNA-binding transcriptional regulator (Q310var.) source 1..309 mol_type protein organism synthetic construct MASETSSPKKRATTLKDIAQATQLSVSTVSRALANNASIPESTRIRVVEAAQKLNYRPNAQARALRKSRTDTIGVIIPNIENPYFSSLAASIQKAAREAGVSTILSNSEENPELLGQTLAIMDDQRLDGIIVVPHIQSEEQVTDLVDRGVPVVLADRSFVNSSIPSVTSDPVPGMTEAVDLLLAADVQLGYLAGPQDTSTGQLRLNTFEKLCVDRGIVGASVYYGGYRQESGYDGIKVLIKQGANAIIAGDSMMTIGALLALHEMNLKIGEDVQLIGFDNNPIFRLQNPPLSIIDQHVQEIGKRAFEIL 1110 DNA PAT misc_feature 1..1110 note snythetic_coding gene LacI family DNA-binding transcriptionalregulator (Q310 var.) source 1..1110 mol_type other DNA organism synthetic construct atggcttccgaaacctccagcccgaagaagcgggccaccacgctcaaagacatcgcgcaagcaacacagctttcagtcagcacggtgtcccgggcattggccaacaacgcgagcattccggaatccacacgcatccgagtggttgaagccgctcaaaagctgaactaccgtcccaatgcccaagctcgtgcattgcggaagtcgaggacagacaccatcggtgtcatcattccaaacattgagaacccatatttctcctcactagcagcatcgattcaaaaagctgctcgtgaagctggggtgtccaccattttgtccaactctgaagaaaacccagagctgcttggtcagactttggcgatcatggatgaccaacgcctcgatggaatcatcgtggtgccacacattcagtcagaggaacaagtcactgacttggttgacaggggagtgccagtagtgctggcagaccgtagttttgttaactcgtctattccttcggttacctcagatccagttccgggcatgactgaagctgtggacttactcctggcagctgacgtgcaattgggctaccttgccggcccgcaggatacttccactggtcagctgcgtcttaacacttttgaaaaactatgcgtggaccgcggcatcgtcggagcatctgtctattacggtggctaccgccaagaatctggatatgacggcatcaaggtgctgatcaagcagggagccaatgcgattatcgctggtgactccatgatgaccatcggtgcgttgttggctcttcatgagatgaatttgaagatcggtgaggatgtgcagctcattgggtttgataacaacccaattttccggctgcagaatccaccgctgagcatcattgaccagcacgtacaagagatcggtaagcgtgcgtttgagattctgtagaagctgatcaatggggacaccgcgcaaaaatctgtggtgattccaacgcagctcagcatcaatggatcaacggcggtttcccaaaaggccgccgcaaaagcagcaaaagcagcccaaaaagcagccgcgaaagccgcacagaacacgcaacacgaggtgagcctagatggtgaactctga 369 AA PAT REGION 1..369 note snythetic_LacI family DNA-binding transcriptional regulator(wild) source 1..369 mol_type protein organism synthetic construct MASETSSPKKRATTLKDIAQATQLSVSTVSRALANNASIPESTRIRVVEAAQKLNYRPNAQARALRKSRTDTIGVIIPNIENPYFSSLAASIQKAAREAGVSTILSNSEENPELLGQTLAIMDDQRLDGIIVVPHIQSEEQVTDLVDRGVPVVLADRSFVNSSIPSVTSDPVPGMTEAVDLLLAADVQLGYLAGPQDTSTGQLRLNTFEKLCVDRGIVGASVYYGGYRQESGYDGIKVLIKQGANAIIAGDSMMTIGALLALHEMNLKIGEDVQLIGFDNNPIFRLQNPPLSIIDQHVQEIGKRAFEILQKLINGDTAQKSVVIPTQLSINGSTAVSQKAAAKAAKAAQKAAAKAAQNTQHEVSLDGEL 1110 DNA PAT misc_feature 1..1110 note snythetic_gene coding LacI family DNA-binding transcriptionalregulator (wild) source 1..1110 mol_type other DNA organism synthetic construct atggcttccgaaacctccagcccgaagaagcgggccaccacgctcaaagacatcgcgcaagcaacacagctttcagtcagcacggtgtcccgggcattggccaacaacgcgagcattccggaatccacacgcatccgagtggttgaagccgctcaaaagctgaactaccgtcccaatgcccaagctcgtgcattgcggaagtcgaggacagacaccatcggtgtcatcattccaaacattgagaacccatatttctcctcactagcagcatcgattcaaaaagctgctcgtgaagctggggtgtccaccattttgtccaactctgaagaaaacccagagctgcttggtcagactttggcgatcatggatgaccaacgcctcgatggaatcatcgtggtgccacacattcagtcagaggaacaagtcactgacttggttgacaggggagtgccagtagtgctggcagaccgtagttttgttaactcgtctattccttcggttacctcagatccagttccgggcatgactgaagctgtggacttactcctggcagctgacgtgcaattgggctaccttgccggcccgcaggatacttccactggtcagctgcgtcttaacacttttgaaaaactatgcgtggaccgcggcatcgtcggagcatctgtctattacggtggctaccgccaagaatctggatatgacggcatcaaggtgctgatcaagcagggagccaatgcgattatcgctggtgactccatgatgaccatcggtgcgttgttggctcttcatgagatgaatttgaagatcggtgaggatgtgcagctcattgggtttgataacaacccaattttccggctgcagaatccaccgctgagcatcattgaccagcacgtacaagagatcggtaagcgtgcgtttgagattctgcagaagctgatcaatggggacaccgcgcaaaaatctgtggtgattccaacgcagctcagcatcaatggatcaacggcggtttcccaaaaggccgccgcaaaagcagcaaaagcagcccaaaaagcagccgcgaaagccgcacagaacacgcaacacgaggtgagcctagatggtgaactctga 36 DNA PAT misc_feature 1..36 note snythetic_1F primer source 1..36 mol_type other DNA organism synthetic construct attcgagctcggtacccgaaaacccagagctgcttg 31 DNA PAT misc_feature 1..31 note snythetic_2R primer source 1..31 mol_type other DNA organism synthetic construct cattgatcagcttctacagaatctcaaacgc 31 DNA PAT misc_feature 1..31 note snythetic_3F primer source 1..31 mol_type other DNA organism synthetic construct gcgtttgagattctgtagaagctgatcaatg 34 DNA PAT misc_feature 1..34 note snythetic_4R primer source 1..34 mol_type other DNA organism synthetic construct cgactctagaggatcccccctgtgcctgcctgcg 17 DNA PAT misc_feature 1..17 note snythetic_5F primer source 1..17 mol_type other DNA organism synthetic construct gctgctcgtgaagctgg 18 DNA PAT misc_feature 1..18 note snythetic_6R primer source 1..18 mol_type other DNA organism synthetic construct gaacccacctgagcattc 35 DNA PAT misc_feature 1..35 note snythetic_7F primer source 1..35 mol_type other DNA organism synthetic construct attcgagctcggtacccccagttcggtcacaagac 32 DNA PAT misc_feature 1..32 note snythetic_8R primer source 1..32 mol_type other DNA organism synthetic construct gctttttgggctgcttcgcttcttcgggctgg 32 DNA PAT misc_feature 1..32 note snythetic_9F primer source 1..32 mol_type other DNA organism synthetic construct ccagcccgaagaagcgaagcagcccaaaaagc 33 DNA PAT misc_feature 1..33 note snythetic_10R primer source 1..33 mol_type other DNA organism synthetic construct gactctagaggatccccggacaacgccttggcg 18 DNA PAT misc_feature 1..18 note snythetic_11F primer source 1..18 mol_type other DNA organism synthetic construct gcaaggcgatggaacgtc 17 DNA PAT misc_feature 1..17 note snythetic_12R primer source 1..17 mol_type other DNA organism synthetic construct ctcatccaagtggtgcg 38 DNA PAT misc_feature 1..38 note snythetic_13F primer source 1..38 mol_type other DNA organism synthetic construct tgaattcgagctcggtacccttgaacggaattgggtgg 38 DNA PAT misc_feature 1..38 note snythetic_14R primer source 1..38 mol_type other DNA organism synthetic construct cccaggtggcatcggtaccttcacccagcgccacgcag 39 DNA PAT misc_feature 1..39 note snythetic_15F primer source 1..39 mol_type other DNA organism synthetic construct cgctgggtgaaggtaccgatgccacctgggttggtcaag 38 DNA PAT misc_feature 1..38 note snythetic_16R primer source 1..38 mol_type other DNA organism synthetic construct gtcgactctagaggatccccggacaaggaatggagaga 18 DNA PAT misc_feature 1..18 note snythetic_17F primer source 1..18 mol_type other DNA organism synthetic construct cttaccgttgttgccctt 19 DNA PAT misc_feature 1..19 note snythetic_18R primer source 1..19 mol_type other DNA organism synthetic construct ctccttcacccacatcatt 1205 AA PAT REGION 1..1205 note snythetic_odhA protein (wild) source 1..1205 mol_type protein organism synthetic construct MFQQFQKDPKSVDKEWRELFEAQGGPNATPATTEAQPSAPKESAKPAPKAAPAAKAAPRVETKPAAKTAPKAKESSVPQQPKLPEPGQTPIRGIFKSIAKNMDISLEIPTATSVRDMPARLMFENRAMVNDQLKRTRGGKISFTHIIGYAMVKAVMAHPDMNNSYDVIDGKPTLIVPEHINLGLAIDLPQKDGSRALVVAAIKETEKMNFSEFLAAYEDIVTRSRKGKLTMDDYQGVTVSLTNPGGIGTRHSVPRLTKGQGTIIGVGSMDYPAEFQGASEDRLAELGVGKLVTITSTYDHRVIQGAVSGEFLRTMSRLLTDDSFWDEIFDAMNVPYTPMRWAQDVPNTGVDKNTRVMQLIEAYRSRGHLIADTNPLSWVQPGMPVPDHRDLDIETHSLTIWDLDRTFSVGGFGGKETMTLREVLSRLRAAYTLKVGSEYTHILDRDERTWLQDRLEAGMPKPTQAEQKYILQKLNAAEAFENFLQTKYVGQKRFSLEGAEALIPLMDSAIDTAAGQGLDEVVIGMPHRGRLNVLFNIVGKPLASIFNEFEGQMEQGQIGGSGDVKYHLGSEGQHLQMFGDGEIKVSLTANPSHLEAVNPVMEGIVRAKQDYLDKGVDGKTVVPLLLHGDAAFAGLGIVPETINLAKLRGYDVGGTIHIVVNNQIGFTTTPDSSRSMHYATDYAKAFGCPVFHVNGDDPEAVVWVGQLATEYRRRFGKDVFIDLVCYRLRGHNEADDPSMTQPKMYELITGRETVRAQYTEDLLGRGDLSNEDAEAVVRDFHDQMESVFNEVKEGGKKQAEAQTGITGSQKLPHGLETNISREELLELGQAFANTPEGFNYHPRVAPVAKKRVSSVTEGGIDWAWGELLAFGSLANSGRLVRLAGEDSRRGTFTQRHAVAIDPATAEEFNPLHELAQSKGNNGKFLVYNSALTEYAGMGFEYGYSVGNEDSVVAWEAQFGDFANGAQTIIDEYVSSGEAKWGQTSKLILLLPHGYEGQGPDHSSARIERFLQLCAEGSMTVAQPSTPANHFHLLRRHALSDLKRPLVIFTPKSMLRNKAAASAPEDFTEVTKFQSVIDDPNVADAAKVKKVMLVSGKLYYELAKRKEKDGRDDIAIVRIEMLHPIPFNRISEALAGYPNAEEVLFVQDEPANQGPWPFYQEHLPELIPNMPKMRRVSRRAQSSTATGVAKVHQLEEKQLIDEAFEA 3618 DNA PAT misc_feature 1..3618 note snythetic_gene coding odhA protein (wild) source 1..3618 mol_type other DNA organism synthetic construct atgttccagcagttccagaaggaccccaagtccgtggacaaggaatggagagaactctttgaggcgcaggggggaccaaatgctacccccgctacaacagaagcacagccttcagcgcccaaggagtctgcgaaaccagcaccaaaggctgcccctgcagccaaggcagcaccgcgcgtagaaaccaagccggccgccaagaccgcccctaaggccaaggagtcctcagtgccacagcaacctaagcttccggagccaggacaaaccccaatcaggggtattttcaagtccatcgcgaagaacatggatatctccctggaaatcccaaccgcaacctcggttcgcgatatgccagctcgcctcatgttcgaaaaccgcgcgatggtcaacgatcagctcaagcgcacccgcggtggcaagatctccttcacccacatcattggctacgccatggtgaaggcagtcatggctcacccggacatgaacaactcctacgacgtcatcgacggcaagccaaccctgatcgtgcctgagcacatcaacctgggccttgccatcgaccttcctcagaaggacggctcccgcgcacttgtcgtagcagccatcaaggaaaccgagaagatgaacttctccgagttcctcgcagcatacgaagacatcgtgacacgctcccgcaagggcaagctcaccatggatgactaccagggcgttaccgtttccttgaccaacccaggtggcatcggtacccgccactctgtcccacgtctgaccaagggccagggcaccatcatcggtgtcggttccatggattacccagcagagttccagggcgcttccgaagaccgccttgcagagctcggcgttggcaagcttgtcaccatcacctccacctacgatcaccgcgtgatccagggtgctgtgtccggtgaattcctgcgtaccatgtctcgcctgctcaccgatgattccttctgggatgagatcttcgacgcaatgaacgttccttacaccccaatgcgttgggcacaggacgttccaaacaccggtgttgataagaacacccgcgtcatgcagctcattgaggcataccgctcccgtggacacctcatcgctgacaccaacccactttcatgggttcagcctggcatgccagttccagaccaccgcgacctcgacatcgagacccacagcctgaccatctgggatctggaccgtaccttcagcgtcggtggcttcggcggcaaggagaccatgaccctgcgcgaggtactgtcccgcctgcgcgctgcctacaccttgaaggtcggctccgaatacacccacatcctggaccgcgacgagcgcacctggctgcaggaccgcctcgaagccggaatgccaaagccaacccaggcagagcagaagtacatcctgcagaagctgaacgccgcagaggctttcgagaacttcctgcagaccaagtacgtcggccagaagcgcttctccctcgaaggtgcagaagctctcatcccactgatggactccgccatcgacaccgccgcaggccagggcctcgacgaagttgtcatcggtatgccacaccgtggtcgcctcaacgtgctgttcaacatcgtgggcaagccactggcatccatcttcaacgagtttgaaggccaaatggagcagggccagatcggtggctccggtgacgtgaagtaccacctcggttccgaaggccagcacctgcagatgttcggcgacggcgagatcaaggtctccctgactgctaacccgtcccacctggaagctgttaacccagtgatggaaggtatcgtccgcgcaaagcaggactacctggacaagggcgtagacggcaagactgttgtgccactgctgctccacggtgacgctgcattcgcaggcctgggcatcgtgccagaaaccatcaacctggctaagctgcgtggctacgacgtcggaggcaccatccacatcgtggtgaacaaccagatcggcttcaccaccaccccagactccagccgctccatgcactacgcaaccgactacgccaaggcattcggctgcccagtcttccacgtcaatggtgatgacccagaggcagttgtctgggttggccagctggcaaccgagtaccgtcgtcgcttcggcaaggacgtcttcatcgacctcgtttgctaccgcctccgcggccacaacgaagctgatgatccttccatgacccagccaaagatgtatgagctcatcaccggccgcgagaccgttcgtgctcagtacaccgaagacctgctcggacgtggagacctctccaacgaagatgcagaagcagtcgtccgcgacttccacgaccagatggaatctgtgttcaacgaagtcaaggaaggcggcaagaagcaggctgaggcacagaccggcatcaccggctcccagaagcttccacacggccttgagaccaacatctcccgtgaagagctcctggaactgggacaggctttcgccaacaccccagaaggcttcaactaccacccacgtgtggctccagttgctaagaagcgcgtctcctctgtcaccgaaggtggcatcgactgggcatggggcgagctcctcgccttcggttccctggctaactccggccgcttggttcgccttgcaggtgaagattcccgccgcggtaccttcacccagcgccacgcagttgccatcgacccagcgaccgctgaagagttcaacccactccacgagcttgcacagtccaagggcaacaacggtaagttcctggtctacaactccgcactgaccgagtacgcaggcatgggcttcgagtacggctactccgtaggaaacgaagactccgtcgttgcatgggaagcacagttcggcgacttcgccaacggcgctcagaccatcatcgatgagtacgtctcctcaggcgaagctaagtggggccagacctccaagctgatccttctgctgcctcacggctacgaaggccagggcccagaccactcttccgcacgtatcgagcgcttcctgcagctgtgcgctgagggttccatgactgttgctcagccatccaccccagcaaaccacttccacctgctgcgtcgtcacgctctgtccgacctgaagcgtccactggttatcttcaccccgaagtccatgctgcgtaacaaggctgctgcctccgcaccagaagacttcactgaggtcaccaagttccaatccgtgatcgacgatccaaacgttgcagatgcagccaaggtgaagaaggtcatgctggtctccggcaagctgtactacgaattggcaaagcgcaaggagaaggacggacgcgacgacatcgcgatcgttcgtatcgaaatgctccacccaattccgttcaaccgcatctccgaggctcttgccggctaccctaacgctgaggaagtcctcttcgttcaggatgagccagcaaaccagggcccatggccgttctaccaggagcacctcccagagctgatcccgaacatgccaaagatgcgccgcgtttcccgccgcgctcagtcctccaccgcaactggtgttgctaaggtgcaccagctggaggagaagcagcttatcgacgaggctttcgaggcttaa