US 63/304,282 2022-01-28 源點生物科技股份有限公司 YUANDIAN BIOLABS CO., LTD CHENG-YAO CHEN ENZYMATIC SYNTHESIS OF POLYNUCLEOTIDE 17 45 DNA PAT source 1..45 mol_type other DNA organism synthetic construct ctcggcctggcacaggtccgttcagtgctgcggcgaccaccgagg 46 DNA PAT source 1..46 mol_type other DNA organism synthetic construct modified_base 46 mod_base OTHER note uracil ctcggcctggcacaggtccgttcagtgctgcggcgaccaccgaggt 47 DNA PAT source 1..47 mol_type other DNA organism synthetic construct modified_base 46 mod_base OTHER note uracil modified_base 47 mod_base OTHER note 3'-O-azidomethyl-2'-deoxyguanosine ctcggcctggcacaggtccgttcagtgctgcggcgaccaccgaggtn 38 DNA PAT source 1..38 mol_type other DNA organism synthetic construct modified_base 22 mod_base OTHER note uracil cagggatccgtgaagctatcctgcgtctaggactaagc 38 DNA PAT source 1..38 mol_type other DNA organism synthetic construct modified_base 22 mod_base i cagggatccgtgaagctatccngcgtctaggactaagc 38 DNA PAT source 1..38 mol_type other DNA organism synthetic construct gcttagtcctagacgcaggatagcttcacggatccctg 223 AA PAT source 1..223 mol_type protein organism Escherichia coli MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEVTRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFVDGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLAWVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRYTANQP 190 AA PAT source 1..190 mol_type protein organism Pyrococcus furiosus IDLRKLTEVQRRLSRKIVEKPIDIAKVKRVGAVDVSYKNNFAKAAFVCVEFPSGEIIKTKTIVTTVEFPYIPTFFFLRETKPILLAVKDENFDVLLVEGHGKAHPRRYGLASHIGVILSKPTIGVAKRLLRGVSKDTYVKVGKAFVSVGNLITLNDAVRIVEKLLDENGYPKPLNIADKLSKRGESREKE 424 AA PAT source 1..424 mol_type protein organism Pyrococcus furiosus MIVDGDLHIHSHYSKAVSKLMTFPIIAENAKLKGLNLVGTGDSLNPHWEKELLKHSKPIDDGTFEVNGVKFILTCEVEDKRRVHHLLIFPTLSQVREFREKVKIYSTNIESEGRPNLNLTAEEIAEMANELDILIGPAHAFTPWTSLYKEYDSLKDAYGDAKIDFLELGLSADSDMADMIKAHHSIPYLSNSDAHSPNPHRLGREFNRFEVKDVTFEEIRKAIKGVGGRKIMLNAGLDPRLGKYHLTACSRCYTKYTLQDAVSLSWKCPKCGGIIKKGVRDRILELADTSEKPKDRPPYVRLAPLAEIIAMVLGKGIESKAVKLLWNRFLREFGSEIRVLIDLPIESIASVHEGVAKAIWAYRNNKLIIVPGGGGKYGEIRIPEEILKAKIEDLNSIEISGGEAELPKPKQRTLLQYIKNKEVN 189 AA PAT source 1..189 mol_type protein organism Thermococcus barophilus SRANVNFKKIAEVQRKLSKRIVEKPLDISRIKTIGAVDVSYKKGKAKAAFVLCSFPSCEILKAKVIETNVDFPYIPTFFFLRETRPVLLAIRGETFDVLLVEGHGKAHPRGYGLASHIGLLLKKPTIGIAKKPLRGYPEGSLVKVGKAYVSVGNLIDLNSAAKIVEMVNENGYPKPLRIADKLSKGAEK 237 AA PAT source 1..237 mol_type protein organism Bacillus subtilis KVFDVHKFDMKKEQDFLQVQFNLKNRINLSPTIHPDSINTCAGVDLAYWEQDGEPYGVCCIIVIDADTKEVIEKVHSMGRISVPYVSGFLAFRELPLIIEAAKKLETEPDVFLFDGNGYLHYNHMGVATHAAFFLGKPTIGIAKTYLKIKGCDFVTPEIEVGAYTDIIIDGEVYGRALRTRRDVKPIFLSCGNYIDLDSSYQITMSLINQESRLPIPVRLADLETHVLRTFYQKNHV 393 AA PAT source 1..393 mol_type protein organism Bacillus pumilus MKEFFADIHIHIGRTRTGRAVKITGARSLTIDQILIEAAESKGMGMIGVIDAQSPEVLEELIEGVEEGRYTELNDGGLSFGQTVLLLGSELEINDDHSKGPIHVLAFMPTLHKMSEFSAWLSLHMKNVHLSSQRLYVDGKILQRKVKELGGLFIPAHIFTPHKSLYGKGVNTSLTEVFDPDLIDAVELGLSCDTSMASQLSELNRYPFLTNSDAHSLGKIAREYTKIKMDHASFAEFKLALEEKDGRAVTGNYGLAPRLGKYYHTTCEKCGVRPHAPDQEKCHVCGHTRMTKGVSERLKELADQESEKGTRPPYVHQLPLQFIPGVGPKTLEKLKAVFHTEMNILHQATEQELKAVLPEKTAGYIIKARKGEVELIAGGGGVYGKVDINHESH 251 AA PAT source 1..251 mol_type protein organism Pyrococcus abyssi MRKVIIKENPSEEEIKELLDLAEKHGGVVTIFARCKVHYEGRAKSELGEGDRIIIIKPDGSFLIHQNKKREPVNWQPPGSKVTFKENSIISIRRRPYERLEVEIIEPYSLVVFLAEDYEELALTGSEAEMANLIFENPRVIEEGFKPIYREKPIRHGIVDVMGVDKDGNIVVLELKRRKADLHAVSQLKRYVDSLKEEYGENVRGILVAPSLTEGAKKLLEKEGLEFRKLEPPKKGNEKRSKQKTLDFFTP 467 AA PAT source 1..467 mol_type protein organism Methanosarcina acetivorans MKVNADLHLHSKYSMACSGKMELPTIASEASKKGMELIGTGDCTHPEWLKDIKKAAISDEEIRIGNTFFIPTTEIEDSNRVHHLLILSSVSKAEELAERIAPFGNLEADGRPTVKLDGCEIAEIAKDLEALIGPCHAFTPWTALYAYHDSLESCYGDMIDYISFIELGLSADSDYADRIEELQRLTFLTNSDAHSPYTNKLAREFTQFNVPDITFEGLRKAILREQGYGPTLNVGFFPEEGKYNRSACIKCFTRYTLPEAEANRWRCPKCGGIMKKGVADRVNELADFETPRHPDHRPPYLHLIPLAEIIQMALGHASIQTKGVKTAWETLVENFGNEVEALIYAEPDALGIVDDRIVNAILAFRKGNVIIHPGGGGQYGWLELPKPLKAEKPGTAEKSAGEKKKTSKTTKREKEKTGAQISFADLESIKSAELAKTTEENTAGEEAKTPEEKGPEKPAGQKSLFDF 249 AA PAT source 1..249 mol_type protein organism Thermococcus gammatolerans MPKVELRENPSPEEIKLLVDLAISSEGVLTIFARCRVHYDGRAKSELGPGDRVIIVKPDGSFLIHQKEKREPVNWQPPGSVVRLELREKPVLISVRRKPRETLEVELDEVYLITVFHAEDYEELALTGSEAEMAELIFENPEVIELGFKPLYREKPIRHGIVDVLGVDRDGNLVVLELKRRRADLHAVSQLKRYVETLREEHENVRGILVAPSLTSGAKKLLEKEGLEFRKLEPPKRDGKSRGRQLRLF 197 AA PAT source 1..197 mol_type protein organism Thermococcus kodakarensis MSEGLFKKLEEVQRKLAERIVERPLEVSKIKTVGAVDVSYRDERARAAFVLCSFPDCELLKQRVVEVDVSFPYIPTFFFLRETRPVLIALGKERPDVLLVEGHGRAHPRGYGLASHIGLVLGIPTIGISKRLLRGTPEGSWVKVGKAYVSVGHLIDLPSAVEVVKTLNKNGYPLPLRIADRLSRGHTSWSGGVNDEH 252 AA PAT source 1..252 mol_type protein organism Thermococcus kodakarensis MSKDKVTVITSPSTEELVSLVNSALLEEAMLTIFARCKVHYDGRAKSELGSGDRVIIVKPDGSFLIHQSKKREPVNWQPPGSRVRLELRENPVLVSIRRKPRETLEVELEEVYMVSVFRAEDYEELALTGSEAEMAELIFENPEVIEPGFKPLFREKAIGTGIVDVLGRDSDGNIVVLELKRRRAELHAVRQLKSYVEILREEYGDKVRGILVAPSLTSGAKRLLEKEGLEFRKLEPPKRDSKKKGRQKTLF