WO 20.130.146940/01 GB 2117283.8 2021-11-30 OXFORD UNIVERSITY INNOVATION LIMITED Switchable polypeptide and its use for gentle affinity purification 19 113 AA PAT VARIANT 77 note wherein X is selected from A, G, S, N or T source 1..113 mol_type protein note SpySwitch variant organism synthetic construct VTTLSGLSGEQGPSGDMTTEEDSATHIHFHKHDEDGRELAGATMELRDCSGKTISTWITDGHVKDFYLYPGKYTFVXTAAPDGHHVATPIEFTINEDGQVTVDGEATEGDPHT 92 AA PAT VARIANT 56 note wherein in X is selected from A, G, S, N or T source 1..92 mol_type protein note Portion of SpySwitch variant organism synthetic construct DSATHIHFHKHDEDGRELAGATMELRDCSGKTISTWITDGHVKDFYLYPGKYTFVXTAAPDGHHVATPIEFTINEDGQVTVDGEATEGDPHT 13 AA PAT source 1..13 mol_type protein note SpyTag organism synthetic construct AHIVMVDAYKPTK 14 AA PAT source 1..14 mol_type protein note SpyTag002 organism synthetic construct VPTIVMVDAYKRYK 16 AA PAT source 1..16 mol_type protein note SpyTag003 organism synthetic construct RGVPHIVMVDAYKRYK 113 AA PAT source 1..113 mol_type protein note SpySwitch organism synthetic construct VTTLSGLSGEQGPSGDMTTEEDSATHIHFHKHDEDGRELAGATMELRDCSGKTISTWITDGHVKDFYLYPGKYTFVATAAPDGHHVATPIEFTINEDGQVTVDGEATEGDPHT 92 AA PAT source 1..92 mol_type protein note Portion of SpySwitch organism synthetic construct DSATHIHFHKHDEDGRELAGATMELRDCSGKTISTWITDGHVKDFYLYPGKYTFVATAAPDGHHVATPIEFTINEDGQVTVDGEATEGDPHT 339 DNA PAT source 1..339 mol_type other DNA note DNA encoding SpySwitch organism synthetic construct gtaaccaccttatcaggtttatcaggtgagcaaggtccgtccggtgatatgacaactgaagaagatagtgctacccatattcacttccataaacatgatgaggacggccgtgagttagctggtgcaactatggagttgcgtgattgctctggtaaaactattagtacatggattacagatggacatgtgaaggatttctacctgtatccaggaaaatatacatttgtcgcgaccgcagcaccagacggtcatcacgtagcaactccaattgaatttacaattaatgaggacggtcaggttactgtagatggtgaagcaactgaaggtgaccctcatact 276 DNA PAT source 1..276 mol_type other DNA note DNA encoding portion of SpySwitch organism synthetic construct gatagtgctacccatattcacttccataaacatgatgaggacggccgtgagttagctggtgcaactatggagttgcgtgattgctctggtaaaactattagtacatggattacagatggacatgtgaaggatttctacctgtatccaggaaaatatacatttgtcgcgaccgcagcaccagacggtcatcacgtagcaactccaattgaatttacaattaatgaggacggtcaggttactgtagatggtgaagcaactgaaggtgaccctcatact 113 AA PAT source 1..113 mol_type protein note SpyDock2.0 organism synthetic construct VTTLSGLSGEQGPSGDMTTEEDSATHIKFHKRDEDGRELAGATMELRDCSGKTISTWISDGHVKDFYLYPGKYTFVATAAPDGHHVATPIEFTVNEDGQVTVDGEATEGDAHT 113 AA PAT source 1..113 mol_type protein note SpyCatcher organism synthetic construct VDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDEDGKELAGATMELRDSSGKTISTWISDGQVKDFYLYPGKYTFVETAAPDGYEVATAITFTVNEQGQVTVNGKATKGDAHI 113 AA PAT source 1..113 mol_type protein note SpyCatcher002 organism synthetic construct VTTLSGLSGEQGPSGDMTTEEDSATHIKFSKRDEDGRELAGATMELRDSSGKTISTWISDGHVKDFYLYPGKYTFVETAAPDGYEVATAITFTVNEQGQVTVNGEATKGDAHT 113 AA PAT source 1..113 mol_type protein note SpyCatcher003 organism synthetic construct VTTLSGLSGEQGPSGDMTTEEDSATHIKFSKRDEDGRELAGATMELRDSSGKTISTWISDGHVKDFYLYPGKYTFVETAAPDGYEVATPIEFTVNEDGQVTVDGEATEGDAHT 7 AA PAT source 1..7 mol_type protein note TEV recognition site organism synthetic construct ENLYFQG 113 AA PAT source 1..113 mol_type protein note SpyDock organism synthetic construct VTTLSGLSGEQGPSGDMTTEEDSATHIKFSKRDEDGRELAGATMELRDCSGKTISTWISDGHVKDFYLYPGKYTFVATAAPDGYEVATPITFTVNEDGQVTVNGEATEGDAHT 136 AA PAT source 1..136 mol_type protein note SpySwitch + His-tag and linkers organism synthetic construct MSYYHHHHHHGSGGSGAMVTTLSGLSGEQGPSGDMTTEEDSATHIHFHKHDEDGRELAGATMELRDCSGKTISTWITDGHVKDFYLYPGKYTFVATAAPDGHHVATPIEFTINEDGQVTVDGEATEGDPHTGSSGS 414 DNA PAT source 1..414 mol_type other DNA note DNA encoding SpySwitch + His-tag and linkers organism synthetic construct atgtcgtactaccatcaccatcaccatcacggtagcggcggtagcggcgccatggtaaccaccttatcaggtttatcaggtgagcaaggtccgtccggtgatatgacaactgaagaagatagtgctacccatattcacttccataaacatgatgaggacggccgtgagttagctggtgcaactatggagttgcgtgattgctctggtaaaactattagtacatggattacagatggacatgtgaaggatttctacctgtatccaggaaaatatacatttgtcgcgaccgcagcaccagacggtcatcacgtagcaactccaattgaatttacaattaatgaggacggtcaggttactgtagatggtgaagcaactgaaggtgaccctcatactggatccagtggtagctaataa 6 AA PAT source 1..6 mol_type protein note Spacer sequence organism synthetic construct GSGGSG 5 AA PAT source 1..5 mol_type protein note Spacer sequence organism synthetic construct GSSGS