093314 JP 2021-184197 2021-11-11 株式会社ヘリオス HEALIOS K.K. 遺伝子改変多能性幹細胞、それ由来の免疫担当細胞、それらの製造方法及びそれらの用途 Genetically-modified pluripotent stem cell, immunocompetent cell derived therefrom, method of producing same and use thereof 19 250 DNA PAT source 1..250 mol_type genomic DNA organism Trichoplusia ni ccctagaaagataatcatattgtgacgtacgttaaagataatcatgcgtaaaattgacgcatgtgttttatcggtctgtatatcgaggtttatttattaatttgaatagatattaagttttattatatttacacttacatactaataataaattcaacaaacaatttatttatgtttatttatttattaaaaaaaaacaaaaactcaaaatttcttctataaagtaacaaaacttttaaacattctctct 307 DNA PAT source 1..307 mol_type genomic DNA organism Trichoplusia ni tatctataacaagaaaatatatatataataagttatcacgtaagtagaacatgaaataacaatataattatcgtatgagttaaatcttaaaagtcacgtaaaagataatcatgcgtcattttgactcacgcggtcgttatagttcaaaatcagtgacacttaccgcattgacaagcacgcctcacgggagctccaagcggcgactgagatgtcctaaatgcacagcgacggattcgcgctatttagaaagagagagcaatatttcaagaatgcatgcgtcaattttacgcagactatctttctaggg 585 DNA PAT misc_feature 1..585 note IRES source 1..585 mol_type other DNA organism synthetic construct gcccctctccctcccccccccctaacgttactggccgaagccgcttggaataaggccggtgtgcgtttgtctatatgtgattttccaccatattgccgtcttttggcaatgtgagggcccggaaacctggccctgtcttcttgacgagcattcctaggggtctttcccctctcgccaaaggaatgcaaggtctgttgaatgtcgtgaaggaagcagttcctctggaagcttcttgaagacaaacaacgtctgtagcgaccctttgcaggcagcggaaccccccacctggcgacaggtgcctctgcggccaaaagccacgtgtataagatacacctgcaaaggcggcacaaccccagtgccacgttgtgagttggatagttgtggaaagagtcaaatggctctcctcaagcgtattcaacaaggggctgaaggatgcccagaaggtaccccattgtatgggatctgatctggggcctcggtgcacatgctttacatgtgtttagtcgaggttaaaaaaacgtctaggccccccgaaccacggggacgtggttttcctttgaaaaacacgatgataatatggccaca 479 DNA PAT source 1..479 mol_type other DNA organism Homo sapiens ccgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtcctt 1086 DNA PAT misc_feature 1..1086 note Modified human CCR2B source 1..1086 mol_type other DNA organism synthetic construct atggctctgtccacatctcgttctcggtttatcagaaataccaacgagagcggtgaagaagtcaccaccttttttgattatgattacggtgctccctgtcataaatttgacgtgaagcaaattggggcccaactcctgcctccgctctactcgctggtgttcatctttggttttgtgggcaacatgctggtcgtcctcatcttaatcaactgcaaaaagctgaagtgcttgactgacatttacctgctcaacctggccatctctgatctgctttttcttattactctcccattgtgggctcactctgctgcaaatgagtgggtctttgggaatgcaatgtgcaaattattcacagggctgtatcacatcggttattttggcggaatcttcttcatcatcctcctgacaatcgatagatacctggctattgtccatgctgtgtttgctttaaaagccaggacggtcacctttggggtggtgacaagtgtgatcacctggttggtggctgtgtttgcttctgtcccaggaatcatctttactaaatgccagaaagaagattctgtttatgtctgtggcccttattttccacgaggatggaataatttccacacaataatgaggaacattttggggctggtcctgccgctgctcatcatggtcatctgctactcgggaatcctgaaaaccctgcttcggtgtcgaaacgagaagaagaggcatagggcagtgagagtcatcttcaccatcatgattgtttactttctcttctggactccctataatattgtcattctcctgaacaccttccaggaattcttcggcctgagtaactgtgaaagcaccagtcaactggaccaagccacgcaggtgacagagactcttgggatgactcactgctgcatcaatcccatcatctatgccttcgttggggagaagttcagaaggtatctctcggtgttcttccgaaagcacatcaccaagcgcttctgcaaacaatgtccagttttctacagggagacagtggatggagtgacttcaacaaacacgccttccactggggagcaggaagtctcggctggtttataa 297 DNA PAT source 1..297 mol_type other DNA organism Homo sapiens atggccctgctactggccctcagcctgctggttctctggacttccccagccccaactctgagtggcaccaatgatgctgaagactgctgcctgtctgtgacccagaaacccatccctgggtacatcgtgaggaacttccactaccttctcatcaaggatggctgcagggtgcctgctgtagtgttcaccacactgaggggccgccagctctgtgcacccccagaccagccctgggtagaacgcatcatccagagactgcagaggacctcagccaagatgaagcgccgcagcagttaa 489 DNA PAT source 1..489 mol_type other DNA organism Homo sapiens atgagaatttcgaaaccacatttgagaagtatttccatccagtgctacttgtgtttacttctaaacagtcattttctaactgaagctggcattcatgtcttcattttgggctgtttcagtgcagggcttcctaaaacagaagccaactgggtgaatgtaataagtgatttgaaaaaaattgaagatcttattcaatctatgcatattgatgctactttatatacggaaagtgatgttcaccccagttgcaaagtaacagcaatgaagtgctttctcttggagttacaagttatttcacttgagtccggagatgcaagtattcatgatacagtagaaaatctgatcatcctagcaaacaacagtttgtcttctaatgggaatgtaacagaatctggatgcaaagaatgtgaggaactggaggaaaaaaatattaaagaatttttgcagagttttgtacatattgtccaaatgttcatcaacacttcttaa 804 DNA PAT source 1..804 mol_type other DNA organism Homo sapiens atggccccgcggcgggcgcgcggctgccggaccctcggtctcccggcgctgctactgctgctgctgctccggccgccggcgacgcggggcatcacgtgccctccccccatgtccgtggaacacgcagacatctgggtcaagagctacagcttgtactccagggagcggtacatttgtaactctggtttcaagcgtaaagccggcacgtccagcctgacggagtgcgtgttgaacaaggccacgaatgtcgcccactggacaacccccagtctcaaatgcattagagaccctgccctggttcaccaaaggccagcgccaccctccacagtaacgacggcaggggtgaccccacagccagagagcctctccccttctggaaaagagcccgcagcttcatctcccagctcaaacaacacagcggccacaacagcagctattgtcccgggctcccagctgatgccttcaaaatcaccttccacaggaaccacagagataagcagtcatgagtcctcccacggcaccccctctcagacaacagccaagaactgggaactcacagcatccgcctcccaccagccgccaggtgtgtatccacagggccacagcgacaccactgtggctatctccacgtccactgtcctgctgtgtgggctgagcgctgtgtctctcctggcatgctacctcaagtcaaggcaaactcccccgctggccagcgttgaaatggaagccatggaggctctgccggtgacttgggggaccagcagcagagatgaagacttggaaaactgctctcaccacctataa 618 DNA PAT source 1..618 mol_type other DNA organism Homo sapiens atggccccgcggcgggcgcgcggctgccggaccctcggtctcccggcgctgctactgctgctgctgctccggccgccggcgacgcggggcatcacgtgccctccccccatgtccgtggaacacgcagacatctgggtcaagagctacagcttgtactccagggagcggtacatttgtaactctggtttcaagcgtaaagccggcacgtccagcctgacggagtgcgtgttgaacaaggccacgaatgtcgcccactggacaacccccagtctcaaatgcattagagaccctgccctggttcaccaaaggccagcgccaccctccacagtaacgacggcaggggtgaccccacagccagagagcctctccccttctggaaaagagcccgcagcttcatctcccagctcaaacaacacagcggccacaacagcagctattgtcccgggctcccagctgatgccttcaaaatcaccttccacaggaaccacagagataagcagtcatgagtcctcccacggcaccccctctcagacaacagccaagaactgggaactcacagcatccgcctcccaccagccgccaggtgtgtatccacagggccacagcgacaccacttaa 405 DNA PAT misc_feature 1..405 note IL-2 signal peptide-fused IL-15 source 1..405 mol_type other DNA organism synthetic construct atgtacaggatgcaactcctgtcttgcattgcactaagtcttgcacttgtcacaaacagtaactgggtgaatgtaataagtgatttgaaaaaaattgaagatcttattcaatctatgcatattgatgctactttatatacggaaagtgatgttcaccccagttgcaaagtaacagcaatgaagtgctttctcttggagttacaagttatttcacttgagtccggagatgcaagtattcatgatacagtagaaaatctgatcatcctagcaaacaacagtttgtcttctaatgggaatgtaacagaatctggatgcaaagaatgtgaggaactggaggaaaaaaatattaaagaatttttgcagagttttgtacatattgtccaaatgttcatcaacacttcttaa 772 DNA PAT misc_feature 1..772 note human CD16a F176V natural variant source 1..772 mol_type other DNA organism synthetic construct atgtggcagctgctcctcccaactgctctgctacttctagtttcagctggcatgcggactgaagatctcccaaaggctgtggtgttcctggagcctcaatggtacagggtgctcgagaaggacagtgtgactctgaagtgccagggagcctactcccctgaggacaattccacacagtggtttcacaatgagagcctcatctcaagccaggcctcgagctacttcattgacgctgccacagtcgacgacagtggagagtacaggtgccagacaaacctctccaccctcagtgacccggtgcagctagaagtccatatcggctggctgttgctccaggcccctcggtgggtgttcaaggaggaagaccctattcacctgaggtgtcacagctggaagaacactgctctgcataaggtcacatatttacagaatggcaaaggcaggaagtattttcatcataattctgacttctacattccaaaagccacactcaaagacagcggctcctacttctgcagggggcttgtcgggagtaaaaatgtgtcttcagagactgtgaacatcaccatcactcaaggtttggcagtgtcaaccatctcatcattctttccacctgggtaccaagtctctttctgcttggtgatggtactcctttttgcagtggacacaggactatatttctctgtgaagacaaacattcgaagctcaacaagagactggaaggaccataaatttaaatggagaaaggaccctcaagacaaatgagggctaa 651 DNA PAT misc_feature 1..651 note modified human NKG2D source 1..651 mol_type other DNA organism synthetic construct atggggtggattcgtggtcggaggtctcgacacagctgggagatgagtgaatttcataattataacttggatctgaagaagagtgatttttcaacacgatggcaaaagcaaagatgtccagtagtcaaaagcaaatgtagagaaaatgcatctccattttttttctgctgcttcatcgctgtagccatgggaatccgtttcattattatggtaacaatatggagtgctgtattcctaaactcattattcaaccaagaagttcaaattcccttgaccgaaagttactgtggcccatgtcctaaaaactggatatgttacaaaaataactgctaccaattttttgatgagagtaaaaactggtatgagagccaggcttcttgtatgtctcaaaatgccagccttctgaaagtatacagcaaagaggaccaggatttacttaaactggtgaagtcatatcattggatgggactggtacacattccaacaaatggatcttggcagtgggaagatggctccattctctcacccaacctactaacaataattgaaatgcagaagggagactgtgcactctatgcctcgagctttaaaggctatatagaaaactgttcaactccaaatacgtacatctgcatgcaaaggactgtgtaa 1416 DNA PAT misc_feature 1..1416 note NKG2D-CAR source 1..1416 mol_type other DNA organism synthetic construct atggccttaccagtgaccgccttgctcctgccgctggccttgctgctccacgccgccaggccgttattcaaccaagaagttcaaattcccttgaccgaaagttactgtggcccatgtcctaaaaactggatatgttacaaaaataactgctaccaattttttgatgagagtaaaaactggtatgagagccaggcttcttgtatgtctcaaaatgccagccttctgaaagtatacagcaaagaggaccaggatttacttaaactggtgaagtcatatcattggatgggactagtacacattccaacaaatggatcttggcagtgggaagatggctccattctctcacccaacctactaacaataattgaaatgcagaagggagactgtgcactctatgcctcgagctttaaaggctatatagaaaactgttcaactccaaatacgtacatctgcatgcaaaggactgtgttcgtgccggtcttcctgccagcgaagcccaccacgacgccagcgccgcgaccaccaacaccggcgcccaccatcgcgtcgcagcccctgtccctgcgcccagaggcgtgccggccagcggcggggggcgcagtgcacacgagggggctggacttcgcctgtgatatctacatctgggcgcccttggccgggacttgtggggtccttctcctgtcactggttatcaccctttactgcaaccacaggaactggaggagaaagaggaaggagaagcagtcagagaccagtcccaaggaatttttgacaatttacgaagatgtcaaggatctgaaaaccaggagaaatcacgagcaggagcagacttttcctggaggggggagcaccatctactctatgatccagtcccagtcttctgctcccacgtcacaagaacctgcatatacattatattcattaattcagccttccaggaagtctggatccaggaagaggaaccacagcccttccttcaatagcactatctatgaagtgattggaaagagtcaacctaaagcccagaaccctgctcgattgagccgcaaagagctggagaactttgatgtttattccagagtgaagttcagcaggagcgcagacgcccccgcgtaccagcagggccagaaccagctctataacgagctcaatctaggacgaagagaggagtacgatgttttggacaagagacgtggccgggaccctgagatggggggaaagccgagaaggaagaaccctcaggaaggcctgtacaatgaactgcagaaagataagatggcggaggcctacagtgagattgggatgaaaggcgagcgccggaggggcaaggggcacgatggcctttaccagggtctcagtacagccaccaaggacacctacgacgcccttcacatgcaggccctgccccctcgctaa 282 DNA PAT misc_feature 1..282 note Modified human DAP10 source 1..282 mol_type other DNA organism synthetic construct atgatccatctgggtcacatcctcttcctgcttttgctcccagtggctgcagctcagacgactccaggagagagatcatcactccctgccttttaccctggcacttcaggctcttgttccggatgtgggtccctctctctgccgctcctggcaggcctcgtggctgctgatgcggtggcatcgctgctcatcgtgggagcagtgtttctttgtgcaagacctaggagatctccagctcaagaagatggcaaagtctacatcaacatgccaggcaggggctga 600 DNA PAT misc_feature 1..600 note Puromycin-resistant gene source 1..600 mol_type other DNA organism synthetic construct atgaccgaatataaacctacggttagattggcgacaagggacgacgtgcctagagctgtacgcacactggcagccgcatttgcggactacccggcaacacgacacaccgtggaccctgatcgccacatagagcgggtcactgaactccaggaattgtttctcacccgagtaggccttgacattggcaaggtatgggtcgcggacgacggtgccgccgttgctgtctggactaccccggaaagcgtagaagctggagctgtattcgcagagatcggtccaaggatggcagagctttctggctccagacttgccgcccaacaacagatggaaggacttctggcgccccataggccgaaagagcctgcgtggtttttggctacagttggcgtttccccagatcatcaaggtaaagggctcggatctgccgtggtgcttcctggggttgaggcagctgagagggccggtgttcctgcgttccttgagacatccgctcctcgcaacttgcctttctatgaaaggctgggttttactgttacggccgatgtagaagtgccagaaggaccacgcacctggtgcatgactagaaagcctggggcatga 1038 DNA PAT misc_feature 1..1038 note Hygromycin resistant gene source 1..1038 mol_type other DNA organism synthetic construct atgaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtggggatcgggagatgggggaggctaactga 375 DNA PAT misc_feature 1..375 note Zeocin-resistant gene source 1..375 mol_type other DNA organism synthetic construct atggcaaagctcacaagcgcagtccctgtgttgacagcaagagatgtcgccggagcagtcgagttttggactgataggttgggattcagcagggacttcgtcgaagatgactttgctggcgtcgtgagagacgatgtgactctgttcatcagcgcagtccaggatcaggttgtccctgacaacaccctcgcttgggtctgggttaggggactcgacgaactctacgcagaatggagcgaagtcgtgtccaccaactttcgggatgcctccggccctgctatgactgagatcggagaacagccttggggaagggaatttgcactgagagatcctgccggaaactgcgtccacttcgtcgctgaagagcaagattga 804 DNA PAT misc_feature 1..804 note Neomycin-resistant gene source 1..804 mol_type other DNA organism synthetic construct atgggatctgccatcgaacaggatgggctccatgcaggatctccagccgcatgggtggaaaggttgttcggatacgactgggctcagcaaactatcggatgctctgatgcagcggtttttcgcctctccgctcagggacgccctgtacttttcgtcaagacagatttgagcggcgcactcaatgaactccaggacgaagctgctagactgtcctggttggctaccactggggttccctgtgctgctgtcttggacgttgttacagaagctggaagagattggctcctgctcggagaagtgcctggacaggacctcctctccagccacctcgcacctgccgagaaagtcagcatcatggcagatgctatgcgccgccttcataccctcgatcccgcaacatgcccattcgatcaccaggccaagcacaggatagagagagcacgcactagaatggaagctggattggtcgatcaagatgatctcgacgaagaacatcagggccttgctcctgccgaattgttcgctcggctcaaagccagaatgcccgatggcgaggatctggtcgttacacatggcgacgcatgtctcccaaacatcatggttgagaacggacggttctctggctttatcgactgcggacggctgggagtcgcagatagataccaagacattgcactggctaccagagatatagcagaggaactcggcggagagtgggcagatagatttctcgttctgtatggtattgctgcacccgatagccaaagaatcgccttttatagactcctggatgagtttttctga 1785 DNA PAT misc_feature 1..1785 note PBase source 1..1785 mol_type other DNA organism synthetic construct atgggaagctccctggacgatgaacatatcctctccgctctgctccaaagcgatgacgaactggtgggcgaggattccgactctgagatttctgaccatgtcagcgaggacgatgtgcagtccgatactgaagaggcatttatcgacgaggtgcatgaagtccagcccacttcctctggaagcgaaatcctggacgagcagaatgtcatcgaacagcctggctcctctctcgcttccaacagaatcctgaccctccctcagaggacaatcagaggcaagaacaagcattgctggtctacaagcaaatccaccaggagaagccgggtgtctgccctcaatatcgtcagaagccaaaggggacccacccggatgtgtaggaacatttacgatcctctgctctgcttcaagctgtttttcactgacgagatcatttctgaaattgtgaagtggacaaacgccgaaatctctctcaagagaagggaaagcatgacaggggctacattcagggacacaaatgaggatgaaatttacgccttctttggcattctggtgatgacagcagtcagaaaagataatcacatgagcacagatgatctcttcgacagatccctgagcatggtgtatgtctctgtgatgtccagagatcggttcgattttctcattagatgcctgcggatggacgataaatccatcaggcccactctcagagagaatgatgtgttcacccctgtgcggaagatttgggatctgttcatccaccagtgcattcaaaattacacccccggcgctcatctcacaatcgacgaacaactcctgggatttcggggcagatgtcccttcagaatgtatattcctaataagccaagcaagtacgggatcaaaattctgatgatgtgtgactctggaaccaagtatatgattaacggaatgccttatctcggcagagggacccaaactaacggcgtgccactcggcgaatactatgtcaaagagctgtccaaacctgtccacggaagctgtagaaatatcacctgcgataactggtttacatccattcccctcgccaagaacctgctccaagaaccttacaagctgaccattgtcggcactgtcaggtccaacaagagggagattccagaagtgctcaagaactccagaagcaggccagtcggcacatccatgttctgtttcgacggaccactgactctcgtgtcttacaagcccaaacctgctaagatggtctatctgctcagctcctgtgatgaggacgcctctattaacgagagcaccggcaagccacaaatggtgatgtactataaccagaccaaggggggagtcgatactctcgaccagatgtgttccgtgatgacatgctccagaaagactaatcggtggcctatggctctcctgtatggcatgattaacattgcctgtatcaacagcttcatcatttatagccacaacgtgtcctctaagggagaaaaggtccaaagcagaaagaaattcatgcggaacctgtatatgagcctcacctcctcttttatgcggaagaggctggaggcacctactctcaaaagatacctgagggataacatcagcaacattctccctaatgaggtccccggaacatccgacgattctactgaggaacctgtgatgaagaaacggacctattgcacatactgtcctagcaagatcagaaggaaggcaaatgcttcctgcaagaaatgtaagaaggtcatttgtagagaacataacattgatatgtgtcagagctgcttctaa