26013-20001.40 US 63/291,235 2021-12-17 Umoja Biopharma, Inc. CYTOTOXIC INNATE LYMPHOID CELL AND USES THEREOF 51 86 AA PAT source 1..86 mol_type protein organism synthetic construct UNSURE 1..86 note IL2RG Common Gamma Chain Intracellular domain ERTMPRIPTLKNLEDLVTEYHGNFSAWSGVSKGLAESLQPDYSERLCLVSEIPPKGGALGEGPGASPCNQHSPYWAPPCYTLKPET 286 AA PAT source 1..286 mol_type protein organism synthetic construct UNSURE 1..286 note IL2RB intracellular domain NCRNTGPWLKKVLKCNTPDPSKFFSQLSSEHGGDVQKWLSSPFPSSSFSPGGLAPEISPLEVLERDKVTQLLLQQDKVPEPASLSSNHSLTSCFTNQGYFFFHLPDALEIEACQVYFTYDPYSEEDPDEGVAGAPTGSSPQPLQPLSGEDDAYCTFPSRDDLLLFSPSLLGGPSPPSTAPGGSGAGEERMPPSLQERVPRDWDPQPLGPPTPGVPDLVDFQPPPELVLREAGEEVPDAGPREGVSFPWSRPPGQGEFRALNARLPLNTDAYLSLQELQGQDPTHLV 195 AA PAT source 1..195 mol_type protein organism synthetic construct UNSURE 1..195 note IL7RB intracellular domain KKRIKPIVWPSLPDHKKTLEHLCKKPRKNLNVSFNPESFLDCQIHRVDDIQARDEVEGFLQDTFPQQLEESEKQRLGGDVQSPNCPSEDVVITPESFGRDSSLTCLAGNVSACDAPILSSSRSLDCRESGKNGPHVYQDLLLSLGTTNSTLPPPFSLQSGILTLNPVAQGQPILTSLGSNQEEAYVTMSSFYQNQ 285 AA PAT source 1..285 mol_type protein organism synthetic construct UNSURE 1..285 note IL21RB intracellular domain SLKTHPLWRLWKKIWAVPSPERFFMPLYKGCSGDFKKWVGAPFTGSSLELGPWSPEVPSTLEVYSCHPPRSPAKRLQLTELQEPAELVESDGVPKPSFWPTAQNSGGSAYSEERDRPYGLVSIDTVTVLDAEGPCTWPCSCEDDGYPALDLDAGLEPSPGLEDPLLDAGTTVLSCGCVSAGSPGLGGPLGSLLDRLKPPLADGEDWAGGLPWGGRSPGGVSESEAGSPLAGLDMDTFDSGFVGSDCSSPVECDFTSPGDEGPPRSYLRQWVVIPPPLSSPGPQAS 107 AA PAT source 1..107 mol_type protein organism synthetic construct UNSURE 1..107 note FKBP domain GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLE 93 AA PAT source 1..93 mol_type protein organism synthetic construct UNSURE 1..93 note FRB domain ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK 93 AA PAT source 1..93 mol_type protein organism synthetic construct UNSURE 1..93 note variant FRB domain (FRB mutant domain) ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK 21 AA PAT source 1..21 mol_type protein organism synthetic construct UNSURE 1..21 note transmembrane (TM) domain VVISVGSMGLIISLLCVYFWL 19 AA PAT source 1..19 mol_type protein organism synthetic construct UNSURE 1..19 note TM domain VAVAGCVFLLISVLLLSGL 25 AA PAT source 1..25 mol_type protein organism synthetic construct UNSURE 1..25 note TM domain PILLTISILSFFSVALLVILACVLW 21 AA PAT source 1..21 mol_type protein organism synthetic construct UNSURE 1..21 note TM domain GWNPHLLLLLLLVIVFIPAFW 21 AA PAT source 1..21 mol_type protein organism synthetic construct UNSURE 1..21 note CD8a signal sequence MALPVTALLLPLALLLHAARP 1554 DNA PAT source 1..1554 mol_type other DNA organism synthetic construct unsure 1..1554 note nucleotide sequence encoding a CAR atggccttaccagtgaccgccttgctcctgccgctggccttgctgctccacgccgccaggccggatgtcgtgatgacccagacccccctcagcctcccagtgtccctcggtgaccaggcttctattagttgcagatccagccagtccctcgtgcactctaacggtaatacctacctgagatggtatctccagaagcccggacagagccctaaggtgctgatctacaaagtctccaaccgggtgtctggagtccctgaccgcttctcagggagcggttccggcaccgacttcaccctgaagatcaaccgggtggaggccgaagacctcggcgtctatttctgctctcagagtacacatgtgccctggaccttcggcggagggaccaagctggagatcaaaagctccgcagacgatgccaagaaagatgccgctaagaaagacgatgctaagaaagacgatgcaaagaaagacggtggcgtgaagctggatgaaaccggaggaggtctcgtccagccaggaggagccatgaagctgagttgcgtgaccagcggattcacctttgggcactactggatgaactgggtgcgacagtccccagagaaggggctcgaatgggtcgctcagttcaggaacaaaccctacaattatgagacatactattcagacagcgtgaagggcaggtttactatcagtagagacgattccaaatctagcgtgtacctgcagatgaacaatctcagggtcgaagatacaggcatctactattgcacaggggcatcctatggtatggagtatctcggtcaggggacaagcgtcacagtcagtttcgtgccggtcttcctgccagcgaagcccaccacgacgccagcgccgcgaccaccaacaccggcgcccaccatcgcgtcgcagcccctgtccctgcgcccagaggcgtgccggccagcggcggggggcgcagtgcacacgagggggctggacttcgcctgtgatatctacatctgggcgcccttggccgggacttgtggggtccttctcctgtcactggttatcaccctttactgcaaccacaggaaccgtttctctgttgttaaacggggcagaaagaaactcctgtatatattcaaacaaccatttatgagaccagtacaaactactcaagaggaagatggctgtagctgccgatttccagaagaagaagaaggaggatgtgaactgagagtgaagttcagcaggagcgcagacgcccccgcgtaccagcagggccagaaccagctctataacgagctcaatctaggacgaagagaggagtacgatgttttggacaagagacgtggccgggaccctgagatggggggaaagccgagaaggaagaaccctcaggaaggcctgtacaatgaactgcagaaagataagatggcggaggcctacagtgagattgggatgaaaggcgagcgccggaggggcaaggggcacgatggcctttaccagggtctcagtacagccaccaaggacacctacgacgcccttcacatgcaggccctgccccctcgctaa 517 AA PAT source 1..517 mol_type protein organism unidentified UNSURE 1..517 note CAR amino acid sequence MALPVTALLLPLALLLHAARPDVVMTQTPLSLPVSLGDQASISCRSSQSLVHSNGNTYLRWYLQKPGQSPKVLIYKVSNRVSGVPDRFSGSGSGTDFTLKINRVEAEDLGVYFCSQSTHVPWTFGGGTKLEIKSSADDAKKDAAKKDDAKKDDAKKDGGVKLDETGGGLVQPGGAMKLSCVTSGFTFGHYWMNWVRQSPEKGLEWVAQFRNKPYNYETYYSDSVKGRFTISRDDSKSSVYLQMNNLRVEDTGIYYCTGASYGMEYLGQGTSVTVSFVPVFLPAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCNHRNRFSVVKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 1560 DNA PAT source 1..1560 mol_type other DNA organism synthetic construct unsure 1..1560 note nucleotide insert gccaccatggccttaccagtgaccgccttgctcctgccgctggccttgctgctccacgccgccaggccggatgtcgtgatgacccagacccccctcagcctcccagtgtccctcggtgaccaggcttctattagttgcagatccagccagtccctcgtgcactctaacggtaatacctacctgagatggtatctccagaagcccggacagagccctaaggtgctgatctacaaagtctccaaccgggtgtctggagtccctgaccgcttctcagggagcggttccggcaccgacttcaccctgaagatcaaccgggtggaggccgaagacctcggcgtctatttctgctctcagagtacacatgtgccctggaccttcggcggagggaccaagctggagatcaaaagctccgcagacgatgccaagaaagatgccgctaagaaagacgatgctaagaaagacgatgcaaagaaagacggtggcgtgaagctggatgaaaccggaggaggtctcgtccagccaggaggagccatgaagctgagttgcgtgaccagcggattcacctttgggcactactggatgaactgggtgcgacagtccccagagaaggggctcgaatgggtcgctcagttcaggaacaaaccctacaattatgagacatactattcagacagcgtgaagggcaggtttactatcagtagagacgattccaaatctagcgtgtacctgcagatgaacaatctcagggtcgaagatacaggcatctactattgcacaggggcatcctatggtatggagtatctcggtcaggggacaagcgtcacagtcagtttcgtgccggtcttcctgccagcgaagcccaccacgacgccagcgccgcgaccaccaacaccggcgcccaccatcgcgtcgcagcccctgtccctgcgcccagaggcgtgccggccagcggcggggggcgcagtgcacacgagggggctggacttcgcctgtgatatctacatctgggcgcccttggccgggacttgtggggtccttctcctgtcactggttatcaccctttactgcaaccacaggaaccgtttctctgttgttaaacggggcagaaagaaactcctgtatatattcaaacaaccatttatgagaccagtacaaactactcaagaggaagatggctgtagctgccgatttccagaagaagaagaaggaggatgtgaactgagagtgaagttcagcaggagcgcagacgcccccgcgtaccagcagggccagaaccagctctataacgagctcaatctaggacgaagagaggagtacgatgttttggacaagagacgtggccgggaccctgagatggggggaaagccgagaaggaagaaccctcaggaaggcctgtacaatgaactgcagaaagataagatggcggaggcctacagtgagattgggatgaaaggcgagcgccggaggggcaaggggcacgatggcctttaccagggtctcagtacagccaccaaggacacctacgacgcccttcacatgcaggccctgccccctcgctaa 496 AA PAT source 1..496 mol_type protein organism synthetic construct UNSURE 1..496 note CAR amino acid sequence without the signal peptide DVVMTQTPLSLPVSLGDQASISCRSSQSLVHSNGNTYLRWYLQKPGQSPKVLIYKVSNRVSGVPDRFSGSGSGTDFTLKINRVEAEDLGVYFCSQSTHVPWTFGGGTKLEIKSSADDAKKDAAKKDDAKKDDAKKDGGVKLDETGGGLVQPGGAMKLSCVTSGFTFGHYWMNWVRQSPEKGLEWVAQFRNKPYNYETYYSDSVKGRFTISRDDSKSSVYLQMNNLRVEDTGIYYCTGASYGMEYLGQGTSVTVSFVPVFLPAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCNHRNRFSVVKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 21 AA PAT source 1..21 mol_type protein organism synthetic construct UNSURE 1..21 note CAR amino acid sequence signal peptide MALPVTALLLPLALLLHAARP 20 DNA PAT source 1..20 mol_type other DNA organism synthetic construct unsure 1..20 note B2M gRNA ggccgagatgtctcgctccg 20 DNA PAT source 1..20 mol_type other DNA organism synthetic construct unsure 1..20 note FKBP12 gRNA cacgcaggtctggccgcgct 20 DNA PAT source 1..20 mol_type other DNA organism synthetic construct unsure 1..20 note FKBP12 gRNA tgctgagccgatgcgcgcgg 20 DNA PAT source 1..20 mol_type other DNA organism synthetic construct unsure 1..20 note FKBP12 gRNA ctcaccgtctcctggggaga 500 DNA PAT source 1..500 mol_type other DNA organism synthetic construct unsure 1..500 note B2M 5 prime homology arm tacccagagaatggagaaaccctgcagggaattcccaagctgtagttataaacagaagttctccttctgctaggtagcattcaaagatcttaatcttctgggtttccgttttctcgaatgaaaaatgcaggtccgagcagttaactggctggggcaccattagcaagtcacttagcatctctggggccagtctgcaaagcgagggggcagccttaatgtgcctccagcctgaagtcctagaatgagcgcccggtgtcccaagctggggcgcgcaccccagatcggagggcgccgatgtacagacagcaaactcacccagtctagtgcatgccttcttaaacatcacgagactctaagaaaaggaaactgaaaacgggaaagtccctctctctaacctggcactgcgtcgctggcttggagacaggtgacggtccctgcgggccttgtcctgattggctgggcacgcgtttaatataagtggaggcgtcgcgctggcgggc 500 DNA PAT source 1..500 mol_type other DNA organism synthetic construct unsure 1..500 note B2M 3 prime homology arm tggccttagctgtgctcgcgctactctctctttctggcctggaggctatccagcgtgagtctctcctaccctcccgctctggtccttcctctcccgctctgcaccctctgtggccctcgctgtgctctctcgctccgtgacttcccttctccaagttctccttggtggcccgccgtggggctagtccagggctggatctcggggaagcggcggggtggcctgggagtggggaagggggtgcgcacccgggacgcgcgctacttgcccctttcggcggggagcaggggagacctttggcctacggcgacgggagggtcgggacaaagtttagggcgtcgataagcgtcagagcgccgaggttgggggagggtttctcttccgctctttcgcggggcctctggctcccccagcgcagctggagtgggggacgggtaggctcgtcccaaaggcgcggcgctgaggtttgtgaacgcgtggaggggcgcttggggtctggggga 1189 DNA PAT source 1..1189 mol_type other DNA organism synthetic construct unsure 1..1189 note EF1alpha promoter cgtgaggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacgcccctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtgaa 1188 DNA PAT source 1..1188 mol_type other DNA organism synthetic construct unsure 1..1188 note EF1alpha promoter cgtgaggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacgcccctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcttgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtga 1205 DNA PAT source 1..1205 mol_type other DNA organism synthetic construct unsure 1..1205 note EF1alpha promoter cgtgaggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcactagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgtggccttgcgcttaaggagccccttcgcctcgtgcttgagttgtggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatcagcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctgcccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagcacaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctccagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtgaaaactacccctaaaagccaaa 347 DNA PAT source 1..347 mol_type other DNA organism synthetic construct unsure 1..347 note MND promoter gaacagagaaacaggagaatatgggccaaacaggatatctgtggtaagcagttcctgccccggctcagggccaagaacagttggaacagcagaatatgggccaaacaggatatctgtggtaagcagttcctgccccggctcagggccaagaacagatggtccccagatgcggtcccgccctcagcagtttctagagaaccatcagatgtttccagggtgccccaaggacctgaaatgaccctgtgccttatttgaactaaccaatcagttcgcttctcgcttctgttcgcgcgcttctgctccccgagctctatataagcagagctcgtttagtgaaccgtcagatc 231 AA PAT source 1..231 mol_type protein organism synthetic construct UNSURE 1..231 note RACR (g) GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLGEGSNTSKENPFLFALEAVVISVGSMGLIISLLCVYFWLERTMPRIPTLKNLEDLVTEYHGNFSAWSGVSKGLAESLQPDYSERLCLVSEIPPKGGALGEGPGASPCNQHSPYWAPPCYTLKPET 20 AA PAT source 1..20 mol_type protein organism synthetic construct UNSURE 1..20 note Signal peptide MPLGLLWLGLALLGALHAQA 108 AA PAT source 1..108 mol_type protein organism synthetic construct UNSURE 1..108 note FKBP domain (FKBP12) GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLGE 37 AA PAT source 1..37 mol_type protein organism synthetic construct UNSURE 1..37 note IL2RG transmembrane domain and ectodomain portion GSNTSKENPFLFALEAVVISVGSMGLIISLLCVYFWL 3354 DNA PAT source 1..3354 mol_type other DNA organism synthetic construct unsure 1..3354 note RACR cgtgaggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacgcccctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcttgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtgagccaccatgccactgggactgctgtggctgggactggccctgctgggcgccctgcacgcccaggccggcgtgcaggtggagacaatcagcccaggcgacggacgcacctttcctaagcggggccagacatgcgtggtgcactacaccggcatgctggaggatggcaagaagttcgactctagcagagataggaacaagccctttaagttcatgctgggcaagcaggaagtgatcagaggctgggaggagggcgtggcccagatgtctgtgggccagagggccaagctgacaatcagcccagactacgcatatggagcaaccggacacccaggaatcatcccacctcacgccacactggtgttcgatgtggagctgctgaagctgggcgagggctctaacaccagcaaggagaatccatttctgttcgcactggaggcagtggtcatctccgtgggctctatgggcctgatcatctccctgctgtgcgtgtacttttggctggagagaacaatgcccaggatccctaccctgaagaacctggaggacctggtgaccgagtatcacggcaatttcagcgcctggtccggcgtgtctaagggactggcagagtccctgcagccagattactctgagcggctgtgcctggtgagcgagatcccacccaagggaggcgccctgggagagggacctggagcaagcccatgcaaccagcactccccatattgggcccctccatgttacaccctgaagcctgagacaggaagcggagcgacgaattttagtctactgaaacaagcgggagacgtggaggaaaaccctggacctatggcactgccagtgaccgccctgctgctgccactggccctgctgctgcacgcagcacgccccatcctgtggcacgaaatgtggcatgaaggcctggaggaggcaagcagactgtattttggcgagagaaatgtgaaaggaatgtttgaggtgctggagccactgcacgcaatgatggagaggggacctcagaccctgaaggagacatcctttaaccaggcctatggcagagacctgatggaggcccaggagtggtgcaggaagtacatgaagagcggaaatgtgaaagacctgctgcaggcctgggatctgtattatcacgtgttcagaaggatcagcaagggcaaggataccatcccttggctgggacacctgctggtgggactgtccggagcctttggcttcatcatcctggtgtacctgctgatcaactgccgcaatacaggcccatggctgaagaaggtgctgaagtgtaacaccccagacccctccaagttcttttctcagctgtcctctgagcacggcggcgatgtgcagaagtggctgagctccccttttccatctagctccttcagccctggaggactggcaccagagatctcccctctggaggtgctggagcgggacaaggtgacccagctgctgctgcagcaggataaggtgcccgagcctgcctccctgtctagcaaccacagcctgacctcctgctttacaaatcagggctatttctttttccacctgccagacgcactggagatcgaggcatgtcaggtgtacttcacatatgatccatacagcgaggaggacccagatgagggagtggccggcgcccctaccggatcctctccacagccactgcagccactgtctggcgaggacgatgcctactgtacatttcctagcagagacgatctgctgctgttctccccttctctgctgggaggaccatctccacctagcaccgcacctggaggatccggggcaggggaggagaggatgccaccatctctgcaggagagggtgccaagggactgggatcctcagccactgggacctccaaccccaggagtgcccgacctggtggatttccagccccctccagagctggtgctgagagaggcaggagaggaggtgccagacgcaggaccaagagagggcgtgagctttccatggtccaggccacctggacagggagagttcagagccctgaacgccaggctgccactgaatacagacgcctacctgtctctgcaggagctgcagggccaggatcccacccacctggtgtaaacaataaaagatctttattttcattagatctgtgtgttggttttttgtgtg 429 AA PAT source 1..429 mol_type protein organism synthetic construct UNSURE 1..429 note RACR (b) MALPVTALLLPLALLLHAARPILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISKGKDTIPWLGHLLVGLSGAFGFIILVYLLINCRNTGPWLKKVLKCNTPDPSKFFSQLSSEHGGDVQKWLSSPFPSSSFSPGGLAPEISPLEVLERDKVTQLLLQQDKVPEPASLSSNHSLTSCFTNQGYFFFHLPDALEIEACQVYFTYDPYSEEDPDEGVAGAPTGSSPQPLQPLSGEDDAYCTFPSRDDLLLFSPSLLGGPSPPSTAPGGSGAGEERMPPSLQERVPRDWDPQPLGPPTPGVPDLVDFQPPPELVLREAGEEVPDAGPREGVSFPWSRPPGQGEFRALNARLPLNTDAYLSLQELQGQDPTHLV 21 AA PAT source 1..21 mol_type protein organism synthetic construct UNSURE 1..21 note CD8a Signal peptide MALPVTALLLPLALLLHAARP 29 AA PAT source 1..29 mol_type protein organism synthetic construct UNSURE 1..29 note IL2RB transmembrane domain and ectodomain portion GKDTIPWLGHLLVGLSGAFGFIILVYLLI 25 AA PAT source 1..25 mol_type protein organism synthetic construct UNSURE 1..25 note IL2RB transmembrane domain IPWLGHLLVGLSGAFGFIILVYLLI 753 DNA PAT source 1..753 mol_type other DNA organism synthetic construct unsure 1..753 note RACR (g) Nucleotides atgccactgggactgctgtggctgggactggccctgctgggcgccctgcacgcccaggccggcgtgcaggtggagacaatcagcccaggcgacggacgcacctttcctaagcggggccagacatgcgtggtgcactacaccggcatgctggaggatggcaagaagttcgactctagcagagataggaacaagccctttaagttcatgctgggcaagcaggaagtgatcagaggctgggaggagggcgtggcccagatgtctgtgggccagagggccaagctgacaatcagcccagactacgcatatggagcaaccggacacccaggaatcatcccacctcacgccacactggtgttcgatgtggagctgctgaagctgggcgagggctctaacaccagcaaggagaatccatttctgttcgcactggaggcagtggtcatctccgtgggctctatgggcctgatcatctccctgctgtgcgtgtacttttggctggagagaacaatgcccaggatccctaccctgaagaacctggaggacctggtgaccgagtatcacggcaatttcagcgcctggtccggcgtgtctaagggactggcagagtccctgcagccagattactctgagcggctgtgcctggtgagcgagatcccacccaagggaggcgccctgggagagggacctggagcaagcccatgcaaccagcactccccatattgggcccctccatgttacaccctgaagcctgagaca 1287 DNA PAT source 1..1287 mol_type other DNA organism synthetic construct unsure 1..1287 note RACR (b) nucleotides atggcactgccagtgaccgccctgctgctgccactggccctgctgctgcacgcagcacgccccatcctgtggcacgaaatgtggcatgaaggcctggaggaggcaagcagactgtattttggcgagagaaatgtgaaaggaatgtttgaggtgctggagccactgcacgcaatgatggagaggggacctcagaccctgaaggagacatcctttaaccaggcctatggcagagacctgatggaggcccaggagtggtgcaggaagtacatgaagagcggaaatgtgaaagacctgctgcaggcctgggatctgtattatcacgtgttcagaaggatcagcaagggcaaggataccatcccttggctgggacacctgctggtgggactgtccggagcctttggcttcatcatcctggtgtacctgctgatcaactgccgcaatacaggcccatggctgaagaaggtgctgaagtgtaacaccccagacccctccaagttcttttctcagctgtcctctgagcacggcggcgatgtgcagaagtggctgagctccccttttccatctagctccttcagccctggaggactggcaccagagatctcccctctggaggtgctggagcgggacaaggtgacccagctgctgctgcagcaggataaggtgcccgagcctgcctccctgtctagcaaccacagcctgacctcctgctttacaaatcagggctatttctttttccacctgccagacgcactggagatcgaggcatgtcaggtgtacttcacatatgatccatacagcgaggaggacccagatgagggagtggccggcgcccctaccggatcctctccacagccactgcagccactgtctggcgaggacgatgcctactgtacatttcctagcagagacgatctgctgctgttctccccttctctgctgggaggaccatctccacctagcaccgcacctggaggatccggggcaggggaggagaggatgccaccatctctgcaggagagggtgccaagggactgggatcctcagccactgggacctccaaccccaggagtgcccgacctggtggatttccagccccctccagagctggtgctgagagaggcaggagaggaggtgccagacgcaggaccaagagagggcgtgagctttccatggtccaggccacctggacagggagagttcagagccctgaacgccaggctgccactgaatacagacgcctacctgtctctgcaggagctgcagggccaggatcccacccacctggtg 49 DNA PAT source 1..49 mol_type other DNA organism synthetic construct unsure 1..49 note Synthetic polyA aataaaagatctttattttcattagatctgtgtgttggttttttgtgtg 4354 DNA PAT source 1..4354 mol_type other DNA organism synthetic construct unsure 1..4354 note RACR_B2M ssODN tacccagagaatggagaaaccctgcagggaattcccaagctgtagttataaacagaagttctccttctgctaggtagcattcaaagatcttaatcttctgggtttccgttttctcgaatgaaaaatgcaggtccgagcagttaactggctggggcaccattagcaagtcacttagcatctctggggccagtctgcaaagcgagggggcagccttaatgtgcctccagcctgaagtcctagaatgagcgcccggtgtcccaagctggggcgcgcaccccagatcggagggcgccgatgtacagacagcaaactcacccagtctagtgcatgccttcttaaacatcacgagactctaagaaaaggaaactgaaaacgggaaagtccctctctctaacctggcactgcgtcgctggcttggagacaggtgacggtccctgcgggccttgtcctgattggctgggcacgcgtttaatataagtggaggcgtcgcgctggcgggccgtgaggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacgcccctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcttgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtgagccaccatgccactgggactgctgtggctgggactggccctgctgggcgccctgcacgcccaggccggcgtgcaggtggagacaatcagcccaggcgacggacgcacctttcctaagcggggccagacatgcgtggtgcactacaccggcatgctggaggatggcaagaagttcgactctagcagagataggaacaagccctttaagttcatgctgggcaagcaggaagtgatcagaggctgggaggagggcgtggcccagatgtctgtgggccagagggccaagctgacaatcagcccagactacgcatatggagcaaccggacacccaggaatcatcccacctcacgccacactggtgttcgatgtggagctgctgaagctgggcgagggctctaacaccagcaaggagaatccatttctgttcgcactggaggcagtggtcatctccgtgggctctatgggcctgatcatctccctgctgtgcgtgtacttttggctggagagaacaatgcccaggatccctaccctgaagaacctggaggacctggtgaccgagtatcacggcaatttcagcgcctggtccggcgtgtctaagggactggcagagtccctgcagccagattactctgagcggctgtgcctggtgagcgagatcccacccaagggaggcgccctgggagagggacctggagcaagcccatgcaaccagcactccccatattgggcccctccatgttacaccctgaagcctgagacaggaagcggagcgacgaattttagtctactgaaacaagcgggagacgtggaggaaaaccctggacctatggcactgccagtgaccgccctgctgctgccactggccctgctgctgcacgcagcacgccccatcctgtggcacgaaatgtggcatgaaggcctggaggaggcaagcagactgtattttggcgagagaaatgtgaaaggaatgtttgaggtgctggagccactgcacgcaatgatggagaggggacctcagaccctgaaggagacatcctttaaccaggcctatggcagagacctgatggaggcccaggagtggtgcaggaagtacatgaagagcggaaatgtgaaagacctgctgcaggcctgggatctgtattatcacgtgttcagaaggatcagcaagggcaaggataccatcccttggctgggacacctgctggtgggactgtccggagcctttggcttcatcatcctggtgtacctgctgatcaactgccgcaatacaggcccatggctgaagaaggtgctgaagtgtaacaccccagacccctccaagttcttttctcagctgtcctctgagcacggcggcgatgtgcagaagtggctgagctccccttttccatctagctccttcagccctggaggactggcaccagagatctcccctctggaggtgctggagcgggacaaggtgacccagctgctgctgcagcaggataaggtgcccgagcctgcctccctgtctagcaaccacagcctgacctcctgctttacaaatcagggctatttctttttccacctgccagacgcactggagatcgaggcatgtcaggtgtacttcacatatgatccatacagcgaggaggacccagatgagggagtggccggcgcccctaccggatcctctccacagccactgcagccactgtctggcgaggacgatgcctactgtacatttcctagcagagacgatctgctgctgttctccccttctctgctgggaggaccatctccacctagcaccgcacctggaggatccggggcaggggaggagaggatgccaccatctctgcaggagagggtgccaagggactgggatcctcagccactgggacctccaaccccaggagtgcccgacctggtggatttccagccccctccagagctggtgctgagagaggcaggagaggaggtgccagacgcaggaccaagagagggcgtgagctttccatggtccaggccacctggacagggagagttcagagccctgaacgccaggctgccactgaatacagacgcctacctgtctctgcaggagctgcagggccaggatcccacccacctggtgtaaacaataaaagatctttattttcattagatctgtgtgttggttttttgtgtgtggccttagctgtgctcgcgctactctctctttctggcctggaggctatccagcgtgagtctctcctaccctcccgctctggtccttcctctcccgctctgcaccctctgtggccctcgctgtgctctctcgctccgtgacttcccttctccaagttctccttggtggcccgccgtggggctagtccagggctggatctcggggaagcggcggggtggcctgggagtggggaagggggtgcgcacccgggacgcgcgctacttgcccctttcggcggggagcaggggagacctttggcctacggcgacgggagggtcgggacaaagtttagggcgtcgataagcgtcagagcgccgaggttgggggagggtttctcttccgctctttcgcggggcctctggctcccccagcgcagctggagtgggggacgggtaggctcgtcccaaaggcgcggcgctgaggtttgtgaacgcgtggaggggcgcttggggtctggggga 66 DNA PAT source 1..66 mol_type other DNA organism synthetic construct unsure 1..66 note PQR linker nucleotide ggaagcggagcgacgaattttagtctactgaaacaagcgggagacgtggaggaaaaccctggacct 22 AA PAT source 1..22 mol_type protein organism synthetic construct UNSURE 1..22 note PQR linker GSGATNFSLLKQAGDVEENPGP 22 AA PAT source 1..22 mol_type protein organism synthetic construct UNSURE 1..22 note F2A VKQTLNFDLLKLAGDVESNPGP 20 AA PAT source 1..20 mol_type protein organism synthetic construct UNSURE 1..20 note E2A QCTNYALLKLAGDVESNPGP 24 AA PAT source 1..24 mol_type protein organism synthetic construct UNSURE 1..24 note T2A LEGGGEGRGSLLTCGDVEENPGPR 18 AA PAT source 1..18 mol_type protein organism synthetic construct UNSURE 1..18 note T2A EGRGSLLTCGDVEENPGP 22 AA PAT source 1..22 mol_type protein organism synthetic construct UNSURE 1..22 note P2A GSGATNFSLLKQAGDVEENPGP 106 AA PAT source 1..106 mol_type protein organism synthetic construct UNSURE 1..106 note FKBP domain GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKL 108 AA PAT source 1..108 mol_type protein organism synthetic construct UNSURE 1..108 note FKBP domain GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLGE 90 AA PAT source 1..90 mol_type protein organism synthetic construct UNSURE 1..90 note FRB domain MEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK 230 AA PAT source 1..230 mol_type protein organism synthetic construct UNSURE 1..230 note IL-9RA KLSPRVKRIFYQNVPSPAMFFQPLYSVHNGNFQTWMGAHGAGVLLSQDCAGTPQGALEPCVQEATALLTCGPARPWKSVALEEEQEGPGTRLPGNLSSEDVLPAGCTEWRVQTLAYLPQEDWAPTSLTRPAPPDSEGSRSSSSSSSSNNNNYCALGCYGGWHLSALPGNTQSSGPIPALACGLSCDHQGLETQQGVAWVLAGHCQRPGLHEDLQGMLLPSVLSKARSWTF