P11058WO EP 22382208.1 2022-03-07 FUNDACION NAVARRA PARA LA ASISTENCIA MEDICA EN AFRICA Expression System For The Treatment Of Cancer 88 379 AA PAT REGION 1..379 note hSTING[R]wt source 1..379 mol_type protein organism Homo sapiens MPHSSLHPSIPCPRGHGAQKAALVLLSACLVTLWGLGEPPEHTLRYLVLHLASLQLGLLLNGVCSLAEELRHIHSRYRGSYWRTVRACLGCPLRRGALLLLSIYFYYSLPNAVGPPFTWMLALLGLSQALNILLGLKGLAPAEISAVCEKGNFNVAHGLAWSYYIGYLRLILPELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPNIRFLDKLPQQTGDRAGIKDRVYSNSIYELLENGQRAGTCVLEYATPLQTLFAMSQYSQAGFSREDRLEQAKLFCRTLEDILADAPESQNNCRLIAYQEPADDSSFSLSQEVLRHLRQEEKEEVTVGSLKTSAVPSTSTMSQEPELLISGMEKPLPLRTDFS 379 AA PAT REGION 1..379 note hSTING[H]wt source 1..379 mol_type protein organism Homo sapiens MPHSSLHPSIPCPRGHGAQKAALVLLSACLVTLWGLGEPPEHTLRYLVLHLASLQLGLLLNGVCSLAEELRHIHSRYRGSYWRTVRACLGCPLRRGALLLLSIYFYYSLPNAVGPPFTWMLALLGLSQALNILLGLKGLAPAEISAVCEKGNFNVAHGLAWSYYIGYLRLILPELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPNIRFLDKLPQQTGDHAGIKDRVYSNSIYELLENGQRAGTCVLEYATPLQTLFAMSQYSQAGFSREDRLEQAKLFCRTLEDILADAPESQNNCRLIAYQEPADDSSFSLSQEVLRHLRQEEKEEVTVGSLKTSAVPSTSTMSQEPELLISGMEKPLPLRTDFS 379 AA PAT REGION 1..379 note hSTING[AQ]wt source 1..379 mol_type protein organism Homo sapiens MPHSSLHPSIPCPRGHGAQKAALVLLSACLVTLWGLGEPPEHTLRYLVLHLASLQLGLLLNGVCSLAEELRHIHSRYRGSYWRTVRACLGCPLRRGALLLLSIYFYYSLPNAVGPPFTWMLALLGLSQALNILLGLKGLAPAEISAVCEKGNFNVAHGLAWSYYIGYLRLILPELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPNIRFLDKLPQQTADRAGIKDRVYSNSIYELLENGQRAGTCVLEYATPLQTLFAMSQYSQAGFSREDRLEQAKLFCQTLEDILADAPESQNNCRLIAYQEPADDSSFSLSQEVLRHLRQEEKEEVTVGSLKTSAVPSTSTMSQEPELLISGMEKPLPLRTDFS 283 AA PAT REGION 1..283 note hSTING[H]truncated source 1..283 mol_type protein organism Homo sapiens MPHSSLHPSIPCPRGHGAQKAALVLLSACLVTLWGLGEPPEHTLRYLVLHLASLQLGLLLNGVCSLAEELRHIHSRYRGSYWRTVRACLGCPLRRGALLLLSIYFYYSLPNAVGPPFTWMLALLGLSQALNILLGLKGLAPAEISAVCEKGNFNVAHGLAWSYYIGYLRLILPELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPNIRFLDKLPQQTGDHAGIKDRVYSNSIYELLENGQRNLQMTAASRCPRRFSGTCGRRKRKRLLWAA 1139 DNA PAT misc_feature 1..1139 note hSTING[R]-S162A/G230I/Q266I (hSTINGmut) source 1..1139 mol_type genomic DNA organism Homo sapiens atgccccactccagcctgcatccatccatcccgtgtcccaggggtcacggggcccagaaggcagccttggttctgctgagtgcctgcctggtgaccctttgggggctaggagagccaccagagcacactctccggtacctggtgctccacctagcctccctgcagctgggactgctgttaaacggggtctgcagcctggctgaggagctgcgccacatccactccaggtaccggggcagctactggaggactgtgcgggcctgcctgggctgccccctccgccgtggggccctgttgctgctgtccatctatttctactactccctcccaaatgcggtcggcccgcccttcacttggatgcttgccctcctgggcctctcgcaggcactgaacatcctcctgggcctcaagggcctggccccagctgagatctctgcagtgtgtgaaaaagggaatttcaacgtggcccatgggctggcatgggcatattacatcggatatctgcggctgatcctgccagagctccaggcccggattcgaacttacaatcagcattacaacaacctgctacggggtgcagtgagccagcggctgtatattctcctcccattggactgtggggtgcctgataacctgagtatggctgaccccaacattcgcttcctggataaactgccccagcagaccattgaccgtgctggcatcaaggatcgggtttacagcaacagcatctatgagcttctggagaacgggcagcgggcgggcacctgtgtcctggagtacgccacccccttgatcactttgtttgccatgtcacaatacagtcaagctggctttagccgggaggataggcttgagcaggccaaactcttctgccggacacttgaggacatcctggcagatgcccctgagtctcagaacaactgccgcctcattgcctaccaggaacctgcagatgacagcagcttctcgctgtcccaggaggttctccggcacctgcggcaggaggaaaaggaagaggttactgtgggcagcttgaagacctcagcggtgcccagtacctccacgatgtcccaagagcctgagctcctcatcagtggaatggaaaagcccctccctctccgcacggatttctctta 379 AA PAT REGION 1..379 note hSTING[R]-S162A/G230I/Q266I (hSTINGmut) source 1..379 mol_type protein organism Homo sapiens MPHSSLHPSIPCPRGHGAQKAALVLLSACLVTLWGLGEPPEHTLRYLVLHLASLQLGLLLNGVCSLAEELRHIHSRYRGSYWRTVRACLGCPLRRGALLLLSIYFYYSLPNAVGPPFTWMLALLGLSQALNILLGLKGLAPAEISAVCEKGNFNVAHGLAWAYYIGYLRLILPELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPNIRFLDKLPQQTIDRAGIKDRVYSNSIYELLENGQRAGTCVLEYATPLITLFAMSQYSQAGFSREDRLEQAKLFCRTLEDILADAPESQNNCRLIAYQEPADDSSFSLSQEVLRHLRQEEKEEVTVGSLKTSAVPSTSTMSQEPELLISGMEKPLPLRTDFS 1137 DNA PAT misc_feature 1..1137 note mSTINGwt source 1..1137 mol_type genomic DNA organism Mus musculus atgccatactccaacctgcatccagccatcccacggcccagaggtcaccgctccaaatatgtagccctcatctttctggtggccagcctgatgatcctttgggtggcaaaggatccaccaaatcacactctgaagtacctagcacttcacctagcctcgcacgaacttggactactgttgaaaaacctctgctgtctggctgaagagctgtgccatgtccagtccaggtaccagggcagctactggaaggctgtgcgcgcctgcctgggatgccccatccactgtatggctatgattctactatcgtcttatttctatttcctccaaaacactgctgacatatacctcagttggatgtttggccttctggtcctctataagtccctaagcatgctcctgggccttcagagcttgactccagcggaagtctctgcagtctgtgaagaaaagaagttaaatgttgcccacgggctggcctggtcatactacattgggtacttgcggttgatcttaccagggctccaggcccggatccgaatgttcaatcagctacataacaacatgctcagtggtgcagggagccgaagactgtacatcctctttccattggactgtggggtgcctgacaacctgagtgtagttgaccccaacattcgattccgagatatgctgccccagcaaaacatcgaccgtgctggcatcaagaatcgggtttattccaacagcgtctacgagattctggagaacggacagccagcaggcgtctgtatcctggagtacgccacccccttgcagaccctgtttgccatgtcacaggatgccaaagctggcttcagtcgggaggatcggcttgagcaggctaaactcttctgccggacacttgaggaaatcctggaagatgtccccgagtctcgaaataactgccgcctcattgtctaccaagaacccacagacggaaacagtttctcactgtctcaggaggtgctccggcacattcgtcaggaagaaaaggaggaggttaccatgaatgcccccatgacctcagtggcacctcctccctccgtactgtcccaagagccaagactcctcatcagtggtatggatcagcctctcccactccgcactgacctcatctga 378 AA PAT REGION 1..378 note mSTINGwt source 1..378 mol_type protein organism Mus musculus MPYSNLHPAIPRPRGHRSKYVALIFLVASLMILWVAKDPPNHTLKYLALHLASHELGLLLKNLCCLAEELCHVQSRYQGSYWKAVRACLGCPIHCMAMILLSSYFYFLQNTADIYLSWMFGLLVLYKSLSMLLGLQSLTPAEVSAVCEEKKLNVAHGLAWSYYIGYLRLILPGLQARIRMFNQLHNNMLSGAGSRRLYILFPLDCGVPDNLSVVDPNIRFRDMLPQQNIDRAGIKNRVYSNSVYEILENGQPAGVCILEYATPLQTLFAMSQDAKAGFSREDRLEQAKLFCRTLEEILEDVPESRNNCRLIVYQEPTDGNSFSLSQEVLRHIRQEEKEEVTMNAPMTSVAPPPSVLSQEPRLLISGMDQPLPLRTDLI 540 AA PAT REGION 1..540 note hIL-12sc source 1..540 mol_type protein organism Homo sapiens MCHQQLVISWFSLVFLASPLVAIWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCSGGGGSGGGGSGGGGSRNLPVATPDPGMFPCLHHSQNLLRAVSNMLQKARQTLEFYPCTSEEIDHEDITKDKTSTVEACLPLELTKNESCLNSRETSFITNGSCLASRKTSFMMALCLSSIYEDLKMYQVEFKTMNAKLLMDPKRQIFLDQNMLAVIDELMQALNFNSETVPQKSSLEEPDFYKTKIKLCILLHAFRIRAVTIDRVMSYLNAS 543 AA PAT REGION 1..543 note mIL-12sc source 1..543 mol_type protein organism Mus musculus MGPQKLTISWFAIVLLVSPLMAMWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIGSGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVQRNMDLKFNIKSSSSPPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELALEARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKMKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRSGGGGSGGGGSGGGGSRVIPVSGPARCLSQSRNLLKTTDDMVKTAREKLKHYSCTAEDIDHEDITRDQTSTLKTCLPLELHKNESCLATRETSSTTRGSCLPPQKTSLMMTLCLGSIYEDLKMYQTEFQAINAALQNHNHQQIILDKGMLVAIDELMQSLNHNGETLRQKPPVGEADPYRVKMKLCILLHAFSTRVVTINRVMGYLSSA 13 DNA PAT misc_feature 1..13 note ISRE source 1..13 mol_type other DNA organism synthetic construct agtttcgctttct 212 DNA PAT misc_feature 1..212 note ISREx4 source 1..212 mol_type other DNA organism synthetic construct atctctgcgatgaacctcacagtttcgctttctatctctgcgatgaacctcacagtttcgctttctatctctgcgatgaacctcacagtttcgctttctatctctgcgatgaacctcacagtttcgctttctgtgcctcttatgatctggatctcgagccatggtcgaggcctgtaggcgtgtacggtgggaggcttatataagcagagctc 156 DNA PAT misc_feature 1..156 note ISREx4 short source 1..156 mol_type other DNA organism synthetic construct gctagcagtttcgctttctgctagcagtttcgctttctgctagcagtttcgctttctgctagcagtttcgctttctgtgcctcttatgatctggatctcgagccatggtcgaggcctgtaggcgtgtacggtgggaggcttatataagcagagctc 22 DNA PAT misc_feature 1..22 note miR122 target sequence source 1..22 mol_type other DNA organism synthetic construct caaacaccattgtcacactcca 130 DNA PAT misc_feature 1..130 note miR122 target sequence x5 source 1..130 mol_type other DNA organism synthetic construct atccaaacaccattgtcacactccaagaccaaacaccattgtcacactccaagaccaaacaccattgtcacactccaagaccaaacaccattgtcacactccaagaccaaacaccattgtcacactccac 376 AA PAT REGION 1..376 note thymidine kinase source 1..376 mol_type protein organism Human alphaherpesvirus 1 MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQKMPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTIFLDRHPIAFMLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN 103 DNA PAT misc_feature 1..103 note AAV ITR source 1..103 mol_type genomic DNA organism Adeno-associated dependoparvovirus A catcatcaataatataccttattttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgtgggaacggggcgggtgacgtag 219 DNA PAT misc_feature 1..219 note short IFNb A-promoter source 1..219 mol_type other DNA organism synthetic construct caggagcttgaataaaatgaatattagaagctgttagaataagagaaaatgacagaggaaaactgaaagggagaactgaaagtgggaaattcctctgaggcagaaaggaccatcccttataaatagcacaggccatgaaggaagatcattctcactgcagcctttgacagcctttgcctcatcttgcaggtagcagccgacaccagcctggcttccatc 219 DNA PAT misc_feature 1..219 note short IFNb B-promoter source 1..219 mol_type other DNA organism synthetic construct caggagcttgaataaaatgaatattagaagctgttagaataagagaaaatgacagaggaaaactgaaagggagaactgaaagcaaaaaattcctctgaggcagaaaggaccatcccttataaatagcacaggccatgaaggaagatcattctcactgcagcctttgacagcctttgcctcatcttgcaggtagcagccgacaccagcctggcttccatc 205 DNA PAT misc_feature 1..205 note short IFNb C-promoter source 1..205 mol_type other DNA organism synthetic construct caggagcttgaataaaatgaatattagaagctgttagaataagagaaaatgacagaggaaaactgaaagggagaactgaaagtgggcagaaaggaccatcccttataaatagcacaggccatgaaggaagatcattctcactgcagcctttgacagcctttgcctcatcttgcaggtagcagccgacaccagcctggcttccatc 185 DNA PAT misc_feature 1..185 note short IFNb D-promoter source 1..185 mol_type other DNA organism synthetic construct caggagcttgaataaaatgaatattagaagctgttagaataagagaaaatgacagaggaaaactgaaagggagaactgaaagtgggaaattcctctgaggcagaaaggaccatattctcactgcagcctttgacagcctttgcctcatcttgcaggtagcagccgacaccagcctggcttccatc 110 DNA PAT misc_feature 1..110 note short IFNb E-promoter source 1..110 mol_type other DNA organism synthetic construct ggagaactgaaagtgggaaattcctctgaggcagaaagattctcactgcagcctttgacagcctttgcctcatcttgcaggtagcagccgacaccagcctggcttccatc 556 DNA PAT misc_feature 1..556 note CXCL9-promoter source 1..556 mol_type genomic DNA organism Mus musculus gacacagacatctcgtgaaacatctggaaaaggtagcagggaacgggacagcagtagcttatgcaactaatctagcaattacaatgataggaaactgtagccacggtgagacgaaatggatttaccctcaacaaattaagaagctttgacttgtgaggaaaggcagtttgagtcgccatatagtgtcatgtcccacgtgggaagtacatctccaaccttcattgtacaacattaatatagtcattgtataatctattccacatccaggtagcaactttgcctgggggtggtttcacatcccttactataaactccccgtttatgtgaaatggaagtagaacatgcagaaattccctgggatctgagagtagggttttccccaggacgatcaatttgtggttagtttagttcttctaggtcagctgaggagaccagccaatcagagacgggaaggaaaagggatttcctaaataaatatgatccccaagaacatgctctctaaagacattctcggacttcactccaacacagtgactcaatagaactcagctctgcc 565 DNA PAT misc_feature 1..565 note CXCL10-promoter source 1..565 mol_type genomic DNA organism Mus musculus tctaactgttcactaagttgtatagactttggatctggctaaatttggcgtgtgatttttttttaaagtttattccaagtattttataataaagcatatgaagtaaaaagcaaaaataaacagtgtaaacacaacaaatgtaaaaaccatggttagaacctgacttagatatcagttctgtgttttatttatgagagaaggaagatgagaattaaagccatttccagacttattctgcaaggcactgcatctgatttctcaaacagctcacgctttggaaagtgaaacttacctcactcgttaaaaattaaaaggagcacaagaggggagagggaaattccaagttcatgggtcacaataaacacaagcaatgccctcggtttacaggggacttccctcgggttgcggagccttgctgagtcatctccaaagtcagccaatcaggactcagggagggaaactctttgcagataaatactcctcagcagccggcactcgagaagcgcttcatccaccgctgagagacatcccgagccaaccttccggaagcctccccatcagcacc 571 DNA PAT misc_feature 1..571 note IFNb1-promoter source 1..571 mol_type genomic DNA organism Mus musculus ccaggatgtggggttctctcccaaagggagctatggcccattgatgtgccatttctcttatagtacactattgtaagccccctgggagctcacagagaccctctcccaccatcccactatttcaaagggcagcagctgtgccatcctttcaggatgagcagctactctgcctggcttttcagtggacactttggctgtttgagagttcttttatcttcagggctgtctcctttctgttcttctctcctggatatttctcttcctttgctccagcaattggtgaaactgtacaagattttataaatccttagtttgtatatattttaacccagtacatagcatataaaatagccaggagcttgaataaaatgaatattagaagctgttagaataagagaaaatgacagaggaaaactgaaagggagaactgaaagtgggaaattcctctgaggcagaaaggaccatcccttataaatagcacaggccatgaaggaagatcattctcactgcagcctttgacagcctttgcctcatcttgcaggtagcagccgacaccagcctggcttccatc 202 DNA PAT misc_feature 1..202 note STATx4 source 1..202 mol_type other DNA organism synthetic construct atctctgcgatgaacctcacttcgctagaaatctctgcgatgaacctcacttcgctagaaatatctctgcgatgaacctcacttcgctagaaatctctgcgatgaacctcacttcgctagaagtgcctcttatgatctggatctcgagccatggtcgaggcctgtaggcgtgtacggtgggaggcttatataagcagagctc 208 DNA PAT misc_feature 1..208 note IRSF3x4 source 1..208 mol_type other DNA organism synthetic construct atctctgcgatgaacctcacgaaatagaaactatctctgcgatgaacctcacgaaatagaaactatctctgcgatgaacctcacgaaatagaaactatctctgcgatgaacctcacgaaatagaaactgtgcctcttatgatctggatctcgagccatggtcgaggcctgtaggcgtgtacggtgggaggcttatataagcagagctc 152 DNA PAT misc_feature 1..152 note short IRSF3x4 source 1..152 mol_type other DNA organism synthetic construct gctagcgaaatagaaactgctagcgaaatagaaactgctagcgaaatagaaactgctagcgaaatagaaactgtgcctcttatgatctggatctcgagccatggtcgaggcctgtaggcgtgtacggtgggaggcttatataagcagagctc 200 DNA PAT misc_feature 1..200 note NF Bx4 source 1..200 mol_type other DNA organism synthetic construct atctctgcgatgaacctcacgggactttccatctctgcgatgaacctcacgggactttccatctctgcgatgaacctcacgggactttccatctctgcgatgaacctcacgggactttccgtgcctcttatgatctggatctcgagccatggtcgaggcctgtaggcgtgtacggtgggaggcttatataagcagagctc 144 DNA PAT misc_feature 1..144 note short NF Bx4 source 1..144 mol_type other DNA organism synthetic construct gctagcgggactttccgctagcgggactttccgctagcgggactttccgctagcgggactttccgtgcctcttatgatctggatctcgagccatggtcgaggcctgtaggcgtgtacggtgggaggcttatataagcagagctc 581 DNA PAT misc_feature 1..581 note IFNb-minimal-promoter source 1..581 mol_type other DNA organism synthetic construct ccccaggatgtggggttctctcccaaagggagctatggcccattgatgtgccatttctcttatagtacactattgtaagccccctgggagctcacagagaccctctcccaccatcccactatttcaaagggcagcagctgtgccatcctttcaggatgagcagctactctgcctggcttttcagtggacactttggctgtttgagagttcttttatcttcagggctgtctcctttctgttcttctctcctggatatttctcttcctttgctccagcaattggtgaaactgtacaagattttataaatccttagtttgtatatattttaacccagtacatagcatataaaatagcaggagcttgaataaaatgaatattagaagctgttagaataagagaaaatgacagaggaaaactgaaagggagaactgaaagtgggaaattcctctgaggcagaaaggaccatcccttataaatagcacaggccatgaaggaagatcattctcactgcagcctttgacagcctttgcctcatcttgcaggtagcagccgacaccagcctggcttccatcggatccatg 8 AA PAT VARIANT 2..4 note 2A core consensus sequence motif, Xaa = Any Amino Acid source 1..8 mol_type protein organism synthetic construct DXEXNPGP 16 DNA PAT misc_feature 1..16 note Primer mouse Actin fw source 1..16 mol_type other DNA organism synthetic construct cgcgtccacccgcgag 16 DNA PAT misc_feature 1..16 note Primer mouse Actin rev source 1..16 mol_type other DNA organism synthetic construct cctggtgcctagggcg 20 DNA PAT misc_feature 1..20 note Primer mouse IFNb fw source 1..20 mol_type other DNA organism synthetic construct ccctatggagatgacggaga 20 DNA PAT misc_feature 1..20 note Primer mouse IFNb rev source 1..20 mol_type other DNA organism synthetic construct ctgtctgctggtggagttca 20 DNA PAT misc_feature 1..20 note Primer mouse TNFa fw source 1..20 mol_type other DNA organism synthetic construct cttccagaactccaggcggt 18 DNA PAT misc_feature 1..18 note Primer mouse TNFa rev source 1..18 mol_type other DNA organism synthetic construct ggtttgctacgacgtggg 23 DNA PAT misc_feature 1..23 note Primer mouse STING fw source 1..23 mol_type other DNA organism synthetic construct catactacattgggtacttgcgg 24 DNA PAT misc_feature 1..24 note Primer mouse STING rev source 1..24 mol_type other DNA organism synthetic construct tggaataaacccgattcttgatgc 22 DNA PAT misc_feature 1..22 note Primer mouse scIL12 fw source 1..22 mol_type other DNA organism synthetic construct actggaccaaagggactatgag 20 DNA PAT misc_feature 1..20 note Primer mouse scIL12 rev source 1..20 mol_type other DNA organism synthetic construct tgatgaagaagctggtgctg 20 DNA PAT misc_feature 1..20 note Primer mouse IL6 fw source 1..20 mol_type other DNA organism synthetic construct acaaagccagagtccttcag 20 DNA PAT misc_feature 1..20 note Primer mouse IL6 rev source 1..20 mol_type other DNA organism synthetic construct tggatggtcttggtccttag 22 DNA PAT misc_feature 1..22 note Primer mouse CXCL9 fw source 1..22 mol_type other DNA organism synthetic construct tcgaggaaccctagtgataagg 21 DNA PAT misc_feature 1..21 note Primer mouse CXCL9 rev source 1..21 mol_type other DNA organism synthetic construct tgagggatttgtagtggatcg 20 DNA PAT misc_feature 1..20 note Primer mouse CXCL10 fw source 1..20 mol_type other DNA organism synthetic construct aagtgctgccgtcattttct 20 DNA PAT misc_feature 1..20 note Primer mouse CXCL10 rev source 1..20 mol_type other DNA organism synthetic construct gtggcaatgatctcaacacg 21 DNA PAT misc_feature 1..21 note Primer mouse IL15 fw source 1..21 mol_type other DNA organism synthetic construct cagaggccaactggatagatg 20 DNA PAT misc_feature 1..20 note Primer mouse IL15 rev source 1..20 mol_type other DNA organism synthetic construct gcaattccaggagaaagcag 22 DNA PAT misc_feature 1..22 note Primer mouse IL7 fw source 1..22 mol_type other DNA organism synthetic construct ctgagtgccacattaaagacaa 22 DNA PAT misc_feature 1..22 note Primer mouse IL7 rev source 1..22 mol_type other DNA organism synthetic construct gatttagaaaagcagcttcctt 20 DNA PAT misc_feature 1..20 note Primer human Actin fw source 1..20 mol_type other DNA organism synthetic construct attgccgacaggatgcagaa 20 DNA PAT misc_feature 1..20 note Primer human Actin rev source 1..20 mol_type other DNA organism synthetic construct cggactcgtcatactcctgc 20 DNA PAT misc_feature 1..20 note Primer human IFNb fw source 1..20 mol_type other DNA organism synthetic construct tgcagcagttccagaaggag 20 DNA PAT misc_feature 1..20 note Primer human IFNb rev source 1..20 mol_type other DNA organism synthetic construct agtctcattccagccagtgc 20 DNA PAT misc_feature 1..20 note Primer human TNFa fw source 1..20 mol_type other DNA organism synthetic construct gtgcttgttcctcagcctct 20 DNA PAT misc_feature 1..20 note Primer human TNFa rev source 1..20 mol_type other DNA organism synthetic construct atgggctacaggcttgtcac 20 DNA PAT misc_feature 1..20 note Primer human STING fw source 1..20 mol_type other DNA organism synthetic construct ggtgaccgtgctggcatcaa 20 DNA PAT misc_feature 1..20 note Primer human STING rev source 1..20 mol_type other DNA organism synthetic construct aaagtctgcaagggggtggc 22 DNA PAT misc_feature 1..22 note Primer human scIL12 fw source 1..22 mol_type other DNA organism synthetic construct ccaagtcaaagagtttggagat 22 DNA PAT misc_feature 1..22 note Primer human scIL12 rev source 1..22 mol_type other DNA organism synthetic construct aggtgaaacgtccagaataatt 20 DNA PAT misc_feature 1..20 note Primer human IL6 fw source 1..20 mol_type other DNA organism synthetic construct agtgaggaacaagccagagc 20 DNA PAT misc_feature 1..20 note Primer human IL6 rev source 1..20 mol_type other DNA organism synthetic construct ggtcaggggtggttattgca 21 DNA PAT misc_feature 1..21 note Primer human CXCL9 fw source 1..21 mol_type other DNA organism synthetic construct accaaccaagggactatccac 20 DNA PAT misc_feature 1..20 note Primer human CXCL9 rev source 1..20 mol_type other DNA organism synthetic construct tcaattttctcgcaggaagg 22 DNA PAT misc_feature 1..22 note Primer human CXCL10 fw source 1..22 mol_type other DNA organism synthetic construct tgctgccttatctttctgactc 21 DNA PAT misc_feature 1..21 note Primer human CXCL10 rev source 1..21 mol_type other DNA organism synthetic construct tgatgcaggtacagcgtacag 22 DNA PAT misc_feature 1..22 note Primer human IL15 fw source 1..22 mol_type other DNA organism synthetic construct caaagtaacagcaatgaagtgc 22 DNA PAT misc_feature 1..22 note Primer human IL15 rev source 1..22 mol_type other DNA organism synthetic construct caaactgttgtttgctaggatg 22 DNA PAT misc_feature 1..22 note Primer human IL7 fw source 1..22 mol_type other DNA organism synthetic construct ttcagaaggcacaacaatactg 22 DNA PAT misc_feature 1..22 note Primer human IL7 rev source 1..22 mol_type other DNA organism synthetic construct gttctttagtgcccatcaaaat 21 DNA PAT misc_feature 1..21 note Primer AAV2 ITR fw source 1..21 mol_type other DNA organism synthetic construct ggaacccctagtgatggagtt 16 DNA PAT misc_feature 1..16 note Primer AAV2 ITR rev source 1..16 mol_type other DNA organism synthetic construct cggcctcagtgagcga 20 DNA PAT misc_feature 1..20 note Primer luciferase fw source 1..20 mol_type other DNA organism synthetic construct aaccagcgccattctgatca 20 DNA PAT misc_feature 1..20 note Primer luciferase rev source 1..20 mol_type other DNA organism synthetic construct ccagtgtcttaccggtgtcc 22 DNA PAT misc_feature 1..22 note Primer eGFP fw source 1..22 mol_type other DNA organism synthetic construct atcaaggtgaacttcaagatcc 22 DNA PAT misc_feature 1..22 note Primer eGFP rev source 1..22 mol_type other DNA organism synthetic construct ttacttgtacagctcgtccatg 187 AA PAT source 1..187 mol_type protein organism Homo sapiens MTNKCLLQIALLLCFSTTALSMSYNLLGFLQRSSNFQCQKLLWQLNGRLEYCLKDRMNFDIPEEIKQLQQFQKEDAALTIYEMLQNIFAIFRQDSSSTGWNETIVENLLANVYHQINHLKTVLEEKLEKEDFTRGKLMSSLHLKRYYGRILHYLKAKEYSHCAWTIVRVEILRNFYFINRLTGYLRN 475 AA PAT source 1..475 mol_type protein organism Homo sapiens MAPRRARGCRTLGLPALLLLLLLRPPATRGITCPPPMSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKCIRDGGSGGGGSGGGSGGGGSLQNWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVENLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTSGAPDEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQ 98 AA PAT source 1..98 mol_type protein organism Homo sapiens MNQTAILICCLIFLTLSGIQGVPLSRTVRCTCISISNQPVNPRSLEKLEIIPASQFCPRVEIIATMKKKGEKRCLNPESKAIKNLLKAVSKERSKRSP 21 AA PAT source 1..21 mol_type protein organism synthetic construct GGSGNFDLLKLAGDVESNPGP 266 DNA PAT source 1..266 mol_type other DNA organism synthetic construct acatgtgactttggtcagctagcaaagaattcaaaagatctaaactcgagaaaaggcctacggttaccccggcgggtttctgacatccggcgggtttctgacatccggcgggtttctgacatccggcgggtttctgacatccggcgggtttctgacatccggcgggtttctgacatccggcgggtttctgacatccggcgggtttctgacatccggcgggtttctgacatccggcgggtgactcacaaccccagaaacagacatcc 586 DNA PAT source 1..586 mol_type other DNA organism synthetic construct cccctctccctcccccccccctaacgttactggcgaagccgcttggaataaggccggtgtgcgtttgtctatatgttattttccaccatattgccgtcttttggcaatgtgagggcccggaaacctggccctgtcttcttgacgagcattcctaggggtctttcccctctcgccaaaggaatgcaaggtctgttgaatgtcgtgaaggaagcagttcctctggaagcttcttgaagacaaacaacgtctgtagcgaccctttgcaggcagcggaaccccccacctggcgacaggtgcctctgcggccaaaagccacgtgtataagatacacctgcaaaggcggcacaaccccagtgccacgttgtgagttggatagttgtggaaagagtcaaatggctctcctcaagcgtattcaacaaggggctgaaggatgcccagaaggtaccccattgtatgggatctgatctggggcctcggtacacatgctttacatgtgtttagtcgaggttaaaaaaacgtctaggccccccgaaccacggggacgtggttttcctttgaaaaacacgatgataatatggccacaacc 12 AA PAT source 1..12 mol_type protein organism synthetic construct VQWRIRVAVIRK 418 DNA PAT source 1..418 mol_type genomic DNA organism Homo sapiens ccatataaggagcaggaaggatcccccgccggaacaacccttatttgggcagcaccttatttggagtggcccgatatggcccggccgcttccggctctgggaggagggaagaaggcggagggaggggcaacgcgggaactccggagctgcgcgggtcccggaggccccggcggcggctagagctctaggcttccccgaagcctgggcgcctgggatgcgggcgcgggcgcgggccctagggtgcaggatggaggtgccgggcgctgtcggatggggggcttcacgtcactccgggtcctcccggccggtcctgccatattagggcttcctgcttcccatatatggccatgtacgtcacgacggaggcggacccgtgccgttccagacccttcaaatagaggcggatccggggagtcgc 10 DNA PAT source 1..10 mol_type other DNA organism synthetic construct misc_difference 3..8 ccwwwwwwgg 181 DNA PAT source 1..181 mol_type genomic DNA organism Homo sapiens tgggtgaggttccaatggcaggttagagcccctcgggccaactgccatcctggaaagtagagacagcagtgcccgctgcccagaagagaccagcaagccaaactggagcccccattgcaggctgtcgccatgtggaaagagtaactcacaattgccaataaagtctcatgtggttttatct