WO PCT/US2022/036876 2022-07-12 070816-02782 US 63/221,454 2021-07-13 REGENERON PHARMACEUTICALS, INC. FENG, Yu MEASUREMENT OF THERAPEUTIC PROTEINS CO-ADMINISTERED TO A SUBJECT BY LC-MRM-MS ASSAY 33 10 AA PAT source 1..10 mol_type protein organism synthetic construct DSFSANQEIR 6 AA PAT source 1..6 mol_type protein organism synthetic construct GQQMGR 7 AA PAT source 1..7 mol_type protein organism synthetic construct GGQQMGR 10 AA PAT source 1..10 mol_type protein organism synthetic construct SMTGGQQMGR 11 AA PAT source 1..11 mol_type protein organism synthetic construct ASMTGGQQMGR 15 AA PAT source 1..15 mol_type protein organism synthetic construct DPLIADSFSANQEIR 16 AA PAT source 1..16 mol_type protein organism synthetic construct RPDLIADSFSANQEIR 11 AA PAT source 1..11 mol_type protein organism synthetic construct MASMTGGQQMG 13 AA PAT source 1..13 mol_type protein organism synthetic construct VNVDEVGGEALGR 13 AA PAT source 1..13 mol_type protein organism synthetic construct NVVDEVGGEALGR 8 AA PAT source 1..8 mol_type protein organism synthetic construct VGGEALGR 7 AA PAT source 1..7 mol_type protein organism synthetic construct RKKRKKR 432 AA PAT source 1..432 mol_type protein organism synthetic construct SDTGRPFVEMYSEIPEIIHMTEGRELVIPCRVTSPNITVTLKKFPLDTLIPDGKRIIWDSRKGFIISNATYKEIGLLTCEATVNGHLYKTNYLTHRQTNTIIDVVLSPSHGIELSVGEKLVLNCTARTELNVGIDFNWEYPSSKHQHKKLVNRDLKTQSGSEMKKFLSTLTIDGVTRSDQGLYTCAASSGLMTKKNSTFVRVHEKDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 24 AA PAT source 1..24 mol_type protein organism synthetic construct SDTGRPFVEMYSEIPEIIHMTEGR 14 AA PAT source 1..14 mol_type protein organism synthetic construct YSEIPEIIHMTEGR 6 AA PAT source 1..6 mol_type protein organism synthetic construct NYLTHR 20 AA PAT source 1..20 mol_type protein organism synthetic construct TIIDVVLSPSHGIELSVGEK 5 AA PAT source 1..5 mol_type protein organism synthetic construct QVTLR 18 AA PAT source 1..18 mol_type protein organism synthetic construct DIQMTQSPSTLSASVGDR 316 AA PAT source 1..316 mol_type protein organism synthetic construct DSFSANQEIRYSEVTPYHVTSVWTKGVTPPANFTQGEDVFHAPYVANQGWYDITKTFNGKDDLLCGAATAGNMLHWWFDQNKDQIKRYLEEHPEKQKINFNGEQMFDVKEAIDTKNHQLDSKLFEYFKEKAFPYLSTKHLGVFPDHVIDMFINGYRLSLTNHGPTPVKEGSKDPRGGIFDAVFTRGDQSKLLTSRHDFKEKNLKEISDLIKKELTEGKALGLSHTYANVRINHVINLWGADFDSNGNLKAIYVTDSDSNASIGMKKYFVGVNSAGKVAISAKEIKEDNIGAQVLGLFTLSTGQDSWNQTNHHHHHH 5 AA PAT source 1..5 mol_type protein organism synthetic construct RDPLA 7 AA PAT source 1..7 mol_type protein organism synthetic construct LIRDPLA 9 AA PAT source 1..9 mol_type protein organism synthetic construct VARIANT 1 note X can be any amino acid VARIANT 2 note X can be any amino acid VARIANT 3 note X can be any amino acid XXXLRDPLA 15 AA PAT source 1..15 mol_type protein organism synthetic construct VARIANT 1 note X can be any amino acid VARIANT 2 note X can be any amino acid VARIANT 3 note X can be any amino acid VARIANT 4 note X can be any amino acid VARIANT 5 note X can be any amino acid VARIANT 6 note X can be any amino acid VARIANT 7 note X can be any amino acid VARIANT 8 note X can be any amino acid VARIANT 9 note X can be any amino acid XXXXXXXXXLRDPLA 16 AA PAT source 1..16 mol_type protein organism synthetic construct RDPLIADSFSANQEIR 16 AA PAT source 1..16 mol_type protein organism synthetic construct DRPLIADSFSANQEIR 16 AA PAT source 1..16 mol_type protein organism synthetic construct LRDPLADSFSANQEIR 15 AA PAT source 1..15 mol_type protein organism synthetic construct RDPLADSFSANQEIR 27 AA PAT source 1..27 mol_type protein organism synthetic construct MASMTGGQQMGRDPLIADSFSANQEIR 18 AA PAT source 1..18 mol_type protein organism synthetic construct LIRDPLIADSFSANQEIR 336 AA PAT source 1..336 mol_type protein organism synthetic construct VARIANT 334 note X can be any amino acid VARIANT 335 note X can be any amino acid VARIANT 336 note X can be any amino acid MASMTGGQQMGRDPLIADSFSANQEIRYSEVTPYHVTSVWTKGVTPPANFTQGEDVFHAPYVANQGWYDITKTFNGKDDLLCGAATAGNMLHWWFDQNKDQIKRYLEEHPEKQKINFNGEQMFDVKEAIDTKNHQLDSKLFEYFKEKAFPYLSTKHLGVFPDHVIDMFINGYRLSLTNHGPTPVKEGSKDPRGGIFDAVFTRGDQSKLLTSRHDFKEKNLKEISDLIKKELTEGKALGLSHTYANVRINHVINLWGADFDSNGNLKAIYVTDSDSNASIGMKKYFVGVNSAGKVAISAKEIKEDNIGAQVLGLFTLSTGQDSWNQTNHHHHHHXXX 327 AA PAT source 1..327 mol_type protein organism synthetic construct VARIANT 325 note X can be any amino acid VARIANT 326 note X can be any amino acid VARIANT 327 note X can be any amino acid LIRDPLIADSFSANQEIRYSEVTPYHVTSVWTKGVTPPANFTQGEDVFHAPYVANQGWYDITKTFNGKDDLLCGAATAGNMLHWWFDQNKDQIKRYLEEHPEKQKINFNGEQMFDVKEAIDTKNHQLDSKLFEYFKEKAFPYLSTKHLGVFPDHVIDMFINGYRLSLTNHGPTPVKEGSKDPRGGIFDAVFTRGDQSKLLTSRHDFKEKNLKEISDLIKKELTEGKALGLSHTYANVRINHVINLWGADFDSNGNLKAIYVTDSDSNASIGMKKYFVGVNSAGKVAISAKEIKEDNIGAQVLGLFTLSTGQDSWNQTNHHHHHHXXX 335 AA PAT source 1..335 mol_type protein organism synthetic construct MASMTGGQQMGRDPLADSFSANQEIRYSEVTPYHVTSVWTKGVTPPANFTQGEDVFHAPYVANQGWYDITKTFNGKDDLLCGAATAGNMLHWWFDQNKDQIKRYLEEHPEKQKINFNGEQMFDVKEAIDTKNHQLDSKLFEYFKEKAFPYLSTKHLGVFPDHVIDMFINGYRLSLTNHGPTPVKEGSKDPRGGIFDAVFTRGDQSKLLTSRHDFKEKNLKEISDLIKKELTEGKALGLSHTYANVRINHVINLWGADFDSNGNLKAIYVTDSDSNASIGMKKYFVGVNSAGKVAISAKEIKEDNIGAQVLGLFTLSTGQDSWNQTNHHHHHHVMV