P2024-1275 Shanghai Minwei Biotechnology Co., LTD Shanghai Minwei Biotechnology Co., LTD FUSION PROTEIN HAVING TRIPLE ACTIVITY AND USE THEREOF 23 181 AA PAT source 1..181 mol_type protein note Wild type FGF21 organism synthetic construct HPIPDSSPLLQFGGQVRQRYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRELLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVGSSDPLSMVGPSQGRSPSYAS 181 AA PAT source 1..181 mol_type protein note FGF21 mutant organism synthetic construct HPIPDSSPLLQFGGQVRQVYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRERLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVGSSDPLSMVGNSTGRSPSYAS 424 AA PAT source 1..424 mol_type protein note Fc-FGF21 mutant fusion protein organism synthetic construct ESKYGPPCPPCPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSLGGGGGSGGGGSGGGGSHPIPDSSPLLQFGGQVRQVYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRERLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVGSSDPLSMVGNSTGRSPSYAS 479 AA PAT source 1..479 mol_type protein note 12C1-F fusion protein organism synthetic construct YGEGTFTSDYSILLDKIAQKAFIEWLIAGGPSSGAPPPSGGGGSGGGGSGGGGSAESKYGPPCPPCPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSLGGGGGSGGGGSGGGGSHPIPDSSPLLQFGGQVRQVYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRERLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVGSSDPLSMVGNSTGRSPSYAS 479 AA PAT source 1..479 mol_type protein note 12C3-F fusion protein organism synthetic construct YGEGTFTSDYSIYLDKIAQKAFIEWLIAGGPSSGAPPPSGGGGSGGGGSGGGGSAESKYGPPCPPCPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSLGGGGGSGGGGSGGGGSHPIPDSSPLLQFGGQVRQVYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRERLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVGSSDPLSMVGNSTGRSPSYAS 479 AA PAT source 1..479 mol_type protein note 12C11-F fusion protein organism synthetic construct HGEGTFTSDYSIYLDKIAAKDFIEWLIAGGPSSGAPPPSGGGGSGGGGSGGGGSAESKYGPPCPPCPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSLGGGGGSGGGGSGGGGSHPIPDSSPLLQFGGQVRQVYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRERLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVGSSDPLSMVGNSTGRSPSYAS 479 AA PAT source 1..479 mol_type protein note 12C12-F fusion protein organism synthetic construct HSEGTFTSDYSILLDKIAQKAFIEWLIAGGPSSGAPPPSGGGGSGGGGSGGGGSAESKYGPPCPPCPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSLGGGGGSGGGGSGGGGSHPIPDSSPLLQFGGQVRQVYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRERLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVGSSDPLSMVGNSTGRSPSYAS 39 AA PAT source 1..39 mol_type protein note 12C1 organism synthetic construct YGEGTFTSDYSILLDKIAQKAFIEWLIAGGPSSGAPPPS 39 AA PAT source 1..39 mol_type protein note 12C2 organism synthetic construct HSEGTFTSDYSIYLDKIAQKAFIEWLIAGGPSSGAPPPS 39 AA PAT source 1..39 mol_type protein note 12C3 organism synthetic construct YGEGTFTSDYSIYLDKIAQKAFIEWLIAGGPSSGAPPPS 39 AA PAT source 1..39 mol_type protein note 12C4 organism synthetic construct HSEGTFTSDYSILLDKIAAKDFIEWLIAGGPSSGAPPPS 39 AA PAT source 1..39 mol_type protein note 12C5 organism synthetic construct YGEGTFTSDYSILLDKIAAKDFVQWLIAGGPSSGAPPPS 39 AA PAT source 1..39 mol_type protein note 12C6 organism synthetic construct HGEGTFTSDYSILLDKIAQKDFVQWLIAGGPSSGAPPPS 39 AA PAT source 1..39 mol_type protein note 12C7 organism synthetic construct HSEGTFTSDYSIYLDKIAAKDFIEWLIAGGPSSGAPPPS 39 AA PAT source 1..39 mol_type protein note 12C8 organism synthetic construct YGEGTFTSDYSIYLDKIAAKAFVQWLIAGGPSSGAPPPS 39 AA PAT source 1..39 mol_type protein note 12C9 organism synthetic construct HSEGTFTSDYSILLDKIAAKDFVQWLIAGGPSSGAPPPS 39 AA PAT source 1..39 mol_type protein note 12C10 organism synthetic construct YSEGTFTSDYSIYLDKIAAKAFVQWLIAGGPSSGAPPPS 39 AA PAT source 1..39 mol_type protein note 12C11 organism synthetic construct HGEGTFTSDYSIYLDKIAAKDFIEWLIAGGPSSGAPPPS 39 AA PAT source 1..39 mol_type protein note 12C12 organism synthetic construct HSEGTFTSDYSILLDKIAQKAFIEWLIAGGPSSGAPPPS 39 AA PAT source 1..39 mol_type protein note Polypeptide design template organism synthetic construct SITE 1 note X =H or Y SITE 2 note X =G or S SITE 13 note X= Y or L SITE 19 note X= A or Q SITE 21 note X= A or D SITE 23 note X= V or I SITE 24 note X= A or Q or E XXEGTFTSDYSIXLDKIAXKXFXXWLIAGGPSSGAPPPS 228 AA PAT source 1..228 mol_type protein note Fc organism synthetic construct ESKYGPPCPPCPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSLG 16 AA PAT source 1..16 mol_type protein note linker1 organism synthetic construct GGGGSGGGGSGGGGSA 15 AA PAT source 1..15 mol_type protein note linker2 organism synthetic construct GGGGSGGGGSGGGGS