KR 2023-01-12 P220047WO KR 10-2022-0005303 2022-01-13 툴젠 toolgen 슈반 세포 특이적 프로모터{A Schwann cell specific promoter} 34 1082 DNA PAT misc_feature 1..1082 note Rat MPZ promoter source 1..1082 mol_type other DNA organism synthetic construct aacacagatggcacatatctactctaaatatatgcagagcttcacaaacgtcatacacatacgtgtgtcacacacgcacacacacacccttccacctctgcccttacctttgctgtcccatctagacattatccctcccatccccttatttcccttatcaaaatggctgctccttcaaggttccaaataacactgcttcctggacctgactcctctttcctctgaacttcctgtgttaagtggtattcctagtgcactgtgccttggtagttgttgagattgccctctgcttctcccttctgcctcctcatctagtgatcttgagcttgtagaaagaactgaattaccattctaatacgagcattctcgaactctccaaatagccaccaagcaggacaataggcagtcttgatcatttaaactgctgcatggcaaaaggaatcgaaggatttcttaacagaagtggggggggggggagatctgggcttcttcctggaagtttcctgatagagaaaatcttctgcctgggtagaatctcccaggatgcagggagatggaaaaagttgttcccagaggactttgtagtctacagtgttgtcgtagccatcggaacaacgagacaccctaatttgggagtgctctgaaagaaacttgcctctaggccctagggctctcaggcaaggaggctaagaaggaatcctttgctgtagccttttggatttaggtttctcagcttatctatccctcagagaagtgtgtctatgtcccttttctgtccctctgcctcaccccaccccaacattccaacctagggtagggggaggtcagtatacacaaagccctctgtgtaaggggtggtatgtgtccccccacccccctacccagagtatacaatgccccttctgctccatgcccctgccaccctccccaccacctctcaattgcacatgccaggctgcaattggtcactggctcaggacagccccctcatgctggggatccaggggattttaagcaggttccagaaaacaccactcagttccttgtcccccgctctctccaccccacagacgctctgggcc 1000 DNA PAT misc_feature 1..1000 note human MPZ promoter source 1..1000 mol_type other DNA organism synthetic construct actatgcctggcataaacttcatttattaaagtttattttgtctttaatctctcatataacttagtcttcctgatattgcagctgtgtgtgcccctcttttgtactcccagcattttgttcattactaaaggaagtgtcatggcttattatacttgattgttgatgggtttgtcctctgatcttcccatctccacctccccaaaccaaattttcaactccttgctggaaggacttaatttttattcctctctctattacctgcattctcatactttacatattgctggcacttaatacaattttgtagccttgaaataaattgaaatggacttaaacagcagcatgaagcactgaaggacttcttgacaaacggaaaggtcaggggcttcttgcctggaaatagtccagtggagaaaaacttctgtctgggaagaatcgcacaggatgaagggaggtgcggggaaaaaaactcccataggacttggtcatctcaagaagtctgtaatgcagcccacattagaggagataacaggggatatcctattttcagagttctctgggggaaacctccctctagttcctagggctgtgaggcagcctctctcaggcaaggaggctgaggagaaatccctttttatggcctttaaattgaggttccatatctatccctcagagaagtgtgtctgtgtccctgtttttgtccctctccctcaccaccccccacaacattccagcctggggcagggggaggccagtggacacaaagccctctgtgtatggggtggtatgtgtccccccacccctccacccagactatacaatgccccttctgctccctgcactctgcccccctccccaccacctctcaactgcacatgccaggctgcaattggttactggctgaggacagccccctcatgctggggccctaggggattttaagcaggttccaggaaccccccgttcagttcctggtcccccactttctcaaccccacag 300 DNA PAT misc_feature 1..300 note Schwann cell specific promoter source 1..300 mol_type other DNA organism synthetic construct gcctcaccccaccccaacattccaacctagggtagggggaggtcagtatacacaaagccctctgtgtaaggggtggtatgtgtccccccacccccctacccagagtatacaatgccccttctgctccatgcccctgccaccctccccaccacctctcaattgcacatgccaggctgcaattggtcactggctcaggacagccccctcatgctggggatccaggggattttaagcaggttccagaaaacaccactcagttccttgtcccccgctctctccaccccacagacgctctgggcc 200 DNA PAT misc_feature 1..200 note Schwann cell specific promoter source 1..200 mol_type other DNA organism synthetic construct cagagtatacaatgccccttctgctccatgcccctgccaccctccccaccacctctcaattgcacatgccaggctgcaattggtcactggctcaggacagccccctcatgctggggatccaggggattttaagcaggttccagaaaacaccactcagttccttgtcccccgctctctccaccccacagacgctctgggcc 300 DNA PAT misc_feature 1..300 note Schwann cell specific promoter source 1..300 mol_type other DNA organism synthetic construct gtccctctccctcaccaccccccacaacattccagcctggggcagggggaggccagtggacacaaagccctctgtgtatggggtggtatgtgtccccccacccctccacccagactatacaatgccccttctgctccctgcactctgcccccctccccaccacctctcaactgcacatgccaggctgcaattggttactggctgaggacagccccctcatgctggggccctaggggattttaagcaggttccaggaaccccccgttcagttcctggtcccccactttctcaaccccacag 200 DNA PAT misc_feature 1..200 note Schwann cell specific promoter source 1..200 mol_type other DNA organism synthetic construct cccctccacccagactatacaatgccccttctgctccctgcactctgcccccctccccaccacctctcaactgcacatgccaggctgcaattggttactggctgaggacagccccctcatgctggggccctaggggattttaagcaggttccaggaaccccccgttcagttcctggtcccccactttctcaaccccacag 117 DNA PAT misc_feature 1..117 note Intronic enhancer source 1..117 mol_type other DNA organism synthetic construct gacaatgagactttgtttggtcgcctcctcctaaagaacagaaaagtctataattgttcctccccagagccagcccacacacatagactgcctgggatgactctccctgtgtgggcg 417 DNA PAT misc_feature 1..417 note Schwann cell specific promoter source 1..417 mol_type other DNA organism synthetic construct gacaatgagactttgtttggtcgcctcctcctaaagaacagaaaagtctataattgttcctccccagagccagcccacacacatagactgcctgggatgactctccctgtgtgggcggcctcaccccaccccaacattccaacctagggtagggggaggtcagtatacacaaagccctctgtgtaaggggtggtatgtgtccccccacccccctacccagagtatacaatgccccttctgctccatgcccctgccaccctccccaccacctctcaattgcacatgccaggctgcaattggtcactggctcaggacagccccctcatgctggggatccaggggattttaagcaggttccagaaaacaccactcagttccttgtcccccgctctctccaccccacagacgctctgggcc 317 DNA PAT misc_feature 1..317 note Schwann cell specific promoter source 1..317 mol_type other DNA organism synthetic construct gacaatgagactttgtttggtcgcctcctcctaaagaacagaaaagtctataattgttcctccccagagccagcccacacacatagactgcctgggatgactctccctgtgtgggcgcagagtatacaatgccccttctgctccatgcccctgccaccctccccaccacctctcaattgcacatgccaggctgcaattggtcactggctcaggacagccccctcatgctggggatccaggggattttaagcaggttccagaaaacaccactcagttccttgtcccccgctctctccaccccacagacgctctgggcc 123 DNA PAT misc_feature 1..123 note Intronic enhancer source 1..123 mol_type other DNA organism synthetic construct gacaatggaactttgtttggttgccacctccccctcctgtagaacaaaaaggtctacagttgcccctccctggggccagcccacacacatagtctctctggaatgaccttccttgtgtgggta 423 DNA PAT misc_feature 1..423 note Schwann cell specific promoter source 1..423 mol_type other DNA organism synthetic construct gacaatggaactttgtttggttgccacctccccctcctgtagaacaaaaaggtctacagttgcccctccctggggccagcccacacacatagtctctctggaatgaccttccttgtgtgggtagtccctctccctcaccaccccccacaacattccagcctggggcagggggaggccagtggacacaaagccctctgtgtatggggtggtatgtgtccccccacccctccacccagactatacaatgccccttctgctccctgcactctgcccccctccccaccacctctcaactgcacatgccaggctgcaattggttactggctgaggacagccccctcatgctggggccctaggggattttaagcaggttccaggaaccccccgttcagttcctggtcccccactttctcaaccccacag 323 DNA PAT misc_feature 1..323 note Schwann cell specific promoter source 1..323 mol_type other DNA organism synthetic construct gacaatggaactttgtttggttgccacctccccctcctgtagaacaaaaaggtctacagttgcccctccctggggccagcccacacacatagtctctctggaatgaccttccttgtgtgggtacccctccacccagactatacaatgccccttctgctccctgcactctgcccccctccccaccacctctcaactgcacatgccaggctgcaattggttactggctgaggacagccccctcatgctggggccctaggggattttaagcaggttccaggaaccccccgttcagttcctggtcccccactttctcaaccccacag 417 DNA PAT misc_feature 1..417 note Schwann cell specific promoter source 1..417 mol_type other DNA organism synthetic construct gcctcaccccaccccaacattccaacctagggtagggggaggtcagtatacacaaagccctctgtgtaaggggtggtatgtgtccccccacccccctacccagagtatacaatgccccttctgctccatgcccctgccaccctccccaccacctctcaattgcacatgccaggctgcaattggtcactggctcaggacagccccctcatgctggggatccaggggattttaagcaggttccagaaaacaccactcagttccttgtcccccgctctctccaccccacagacgctctgggccgacaatgagactttgtttggtcgcctcctcctaaagaacagaaaagtctataattgttcctccccagagccagcccacacacatagactgcctgggatgactctccctgtgtgggcg 317 DNA PAT misc_feature 1..317 note Schwann cell specific promoter source 1..317 mol_type other DNA organism synthetic construct cagagtatacaatgccccttctgctccatgcccctgccaccctccccaccacctctcaattgcacatgccaggctgcaattggtcactggctcaggacagccccctcatgctggggatccaggggattttaagcaggttccagaaaacaccactcagttccttgtcccccgctctctccaccccacagacgctctgggccgacaatgagactttgtttggtcgcctcctcctaaagaacagaaaagtctataattgttcctccccagagccagcccacacacatagactgcctgggatgactctccctgtgtgggcg 423 DNA PAT misc_feature 1..423 note Schwann cell specific promoter source 1..423 mol_type other DNA organism synthetic construct gtccctctccctcaccaccccccacaacattccagcctggggcagggggaggccagtggacacaaagccctctgtgtatggggtggtatgtgtccccccacccctccacccagactatacaatgccccttctgctccctgcactctgcccccctccccaccacctctcaactgcacatgccaggctgcaattggttactggctgaggacagccccctcatgctggggccctaggggattttaagcaggttccaggaaccccccgttcagttcctggtcccccactttctcaaccccacaggacaatggaactttgtttggttgccacctccccctcctgtagaacaaaaaggtctacagttgcccctccctggggccagcccacacacatagtctctctggaatgaccttccttgtgtgggta 323 DNA PAT misc_feature 1..323 note Schwann cell specific promoter source 1..323 mol_type other DNA organism synthetic construct cccctccacccagactatacaatgccccttctgctccctgcactctgcccccctccccaccacctctcaactgcacatgccaggctgcaattggttactggctgaggacagccccctcatgctggggccctaggggattttaagcaggttccaggaaccccccgttcagttcctggtcccccactttctcaaccccacaggacaatggaactttgtttggttgccacctccccctcctgtagaacaaaaaggtctacagttgcccctccctggggccagcccacacacatagtctctctggaatgaccttccttgtgtgggta 104 DNA PAT misc_feature 1..104 note simian virus 40 polyadenylation signal source 1..104 mol_type other DNA organism synthetic construct tgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggagatgtgggaggttttttaaa 92 DNA PAT misc_feature 1..92 note synthetic polyadenylation signal source 1..92 mol_type other DNA organism synthetic construct aagaggtaagggtttaagggatggttggttggtggggtattaatgtttaattacctggagcacctgcctgaaatcactttttttcaggttgg 247 DNA PAT misc_feature 1..247 note WPRE3 source 1..247 mol_type other DNA organism synthetic construct aatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttagttcttgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgt 592 DNA PAT misc_feature 1..592 note WPRE source 1..592 mol_type other DNA organism synthetic construct aatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcctg 92 DNA PAT misc_feature 1..92 note MVM intron source 1..92 mol_type other DNA organism synthetic construct aagaggtaagggtttaagggatggttggttggtggggtattaatgtttaattacctggagcacctgcctgaaatcactttttttcaggttgg 23 DNA PAT misc_feature 1..23 note Target Sequence with PAM (5'-3') source 1..23 mol_type other DNA organism synthetic construct ggaccagcccctgaataaactgg 25 DNA PAT misc_feature 1..25 note Target Sequence with PAM (5'-3') source 1..25 mol_type other DNA organism synthetic construct attcaggggctggtccaatgctggg 20 DNA PAT misc_feature 1..20 note SpCas9 forward primer source 1..20 mol_type other DNA organism synthetic construct ggacttctaccccttcctga 19 DNA PAT misc_feature 1..19 note SpCas9 reverse primer source 1..19 mol_type other DNA organism synthetic construct gtgatggtctcctcgctct 22 DNA PAT misc_feature 1..22 note hGAPDH forward primer source 1..22 mol_type other DNA organism synthetic construct gtctcctctgacttcaacagcg 22 DNA PAT misc_feature 1..22 note hGAPDH reverse primer source 1..22 mol_type other DNA organism synthetic construct accaccctgttgctgtagccaa 20 DNA PAT misc_feature 1..20 note rGAPDH forward primer source 1..20 mol_type other DNA organism synthetic construct gaaacccatcaccatcttcc 21 DNA PAT misc_feature 1..21 note rGAPDH reverse primer source 1..21 mol_type other DNA organism synthetic construct gttcacacccatcacaaacat 21 DNA PAT misc_feature 1..21 note rPMP22 forward primer source 1..21 mol_type other DNA organism synthetic construct tccaagttcatttcctgcagg 20 DNA PAT misc_feature 1..20 note rPMP22 reverse primer source 1..20 mol_type other DNA organism synthetic construct ccaggctccctgagatgttc 212 DNA PAT source 1..212 mol_type other DNA organism synthetic construct misc_feature 1..212 note EPS promoter gggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgatccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacag 1431 DNA PAT source 1..1431 mol_type other DNA organism synthetic construct misc_feature 1..1431 note dtTomato atggtgagcaagggcgaggaggtcatcaaagagttcatgcgcttcaaggtgcgcatggagggctccatgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggcggccccctgcccttcgcctgggacatcctgtccccccagttcatgtacggctccaaggcgtacgtgaagcaccccgccgacatccccgattacaagaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggtctggtgaccgtgacccaggactcctccctgcaggacggcacgctgatctacaaggtgaagatgcgcggcaccaacttcccccccgacggccccgtaatgcagaagaagaccatgggctgggaggcctccaccgagcgcctgtacccccgcgacggcgtgctgaagggcgagatccaccaggccctgaagctgaaggacggcggccactacctggtggagttcaagaccatctacatggccaagaagcccgtgcaactgcccggctactactacgtggacaccaagctggacatcacctcccacaacgaggactacaccatcgtggaacagtacgagcgctccgagggccgccaccacctgttcctggggcatggcaccggcagcaccggcagcggcagctccggcaccgcctcctccgaggacaacaacatggccgtcatcaaagagttcatgcgcttcaaggtgcgcatggagggctccatgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggcggccccctgcccttcgcctgggacatcctgtccccccagttcatgtacggctccaaggcgtacgtgaagcaccccgccgacatccccgattacaagaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggtctggtgaccgtgacccaggactcctccctgcaggacggcacgctgatctacaaggtgaagatgcgcggcaccaacttcccccccgacggccccgtaatgcagaagaagaccatgggctgggaggcctccaccgagcgcctgtacccccgcgacggcgtgctgaagggcgagatccaccaggccctgaagctgaaggacggcggccactacctggtggagttcaagaccatctacatggccaagaagcccgtgcaactgcccggctactactacgtggacaccaagctggacatcacctcccacaacgaggactacaccatcgtggaacagtacgagcgctccgagggccgccaccacctgttcctgtacggcatggacgagctgtacaagtag 22 DNA PAT source 1..22 mol_type other DNA organism synthetic construct misc_feature 1..22 note Target Sequence with PAM (5'-3') gaccagcccctgaataaactgg