EP PCT/EP2019/077116 2019-10-07 PCT/EP2019/077116 IT 102018000009282 2018-10-09 METIS PRECISION MEDICINE SB S.R.L. ANTI-MET FAB-FC FOR THE TREATMENT OF A TUMOR AND/OR METASTASIS 43 10 AA PAT REGION 1..10 note VL-CDR1 source 1..10 mol_type protein organism synthetic construct QSVDYDGGSY 000 9 AA PAT REGION 1..9 note VL-CDR3 source 1..9 mol_type protein organism synthetic construct QQSYEDPLT 8 AA PAT REGION 1..8 note VH-CDR1 source 1..8 mol_type protein organism synthetic construct GYTFTSYW 8 AA PAT REGION 1..8 note VH-CDR2 source 1..8 mol_type protein organism synthetic construct INPSSGRT 5 AA PAT REGION 1..5 note VH-CDR3 source 1..5 mol_type protein organism synthetic construct ASRGY 131 AA PAT REGION 1..131 note humanized VL-cl03E08 source 1..131 mol_type protein organism synthetic construct METDTILLWVLLLWVPGSTGDIVLTQSPDSLAVSLGQRATINCKASQSVDYDGGSYMSWFQQKPGQPPKLLIYAASNLESGVPARFSGSGSGTDFTLTISSLQAEDVATYYCQQSYEDPLTFGGGTKVEIK 131 AA PAT REGION 1..131 note humanized VH-cl03E08 source 1..131 mol_type protein organism synthetic construct MGWSYIILFLVATATDGHSQVQLQQSGAEVKKPGASVKLSCKASGYTFTSYWIHWVRQAPGQGLEWIGEINPSSGRTNYNEKFKNRVTVTVDKSTSTAYMELSSLTSEDSAVYYCASRGYWGQGTTLTVSS 107 AA PAT source 1..107 mol_type protein organism Homo sapiens RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC 98 AA PAT source 1..98 mol_type protein organism Homo sapiens ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKV 232 AA PAT REGION 1..232 note First Fc (Knob mutation) source 1..232 mol_type protein organism synthetic construct EPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 246 AA PAT REGION 1..246 note Second Fc (Hole mutations) source 1..246 mol_type protein organism synthetic construct MGWSYIILFLVATATDGHSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 976 AA PAT REGION 1..976 note Decoy Met wt-TAGs source 1..976 mol_type protein organism synthetic construct MKAPAVLAPGILVLLFTLVQRSNGECKEALAKSEMNVNMKYQLPNFTAETPIQNVILHEHHIFLGATNYIYVLNEEDLQKVAEYKTGPVLEHPDCFPCQDCSSKANLSGGVWKDNINMALVVDTYYDDQLISCGSVNRGTCQRHVFPHNHTADIQSEVHCIFSPQIEEPSQCPDCVVSALGAKVLSSVKDRFINFFVGNTINSSYFPDHPLHSISVRRLKETKDGFMFLTDQSYIDVLPEFRDSYPIKYVHAFESNNFIYFLTVQRETLDAQTFHTRIIRFCSINSGLHSYMEMPLECILTEKRKKRSTKKEVFNILQAAYVSKPGAQLARQIGASLNDDILFGVFAQSKPDSAEPMDRSAMCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARRDEYRTEFTTALQRVDLFMGQFSEVLLTSISTFIKGDLTIANLGTSEGRFMQVVVSRSGPSTPHVNFLLDSHPVSPEVIVEHTLNQNGYTLVITGKKITKIPLNGLGCRHFQSCSQCLSAPPFVQCGWCHDKCVRSEECLSGTWTQQICLPAIYKVFPNSAPLEGGTRLTICGWDFGFRRNNKFDLKKTRVLLGNESCTLTLSESTMNTLKCTVGPAMNKHFNMSIIISNGHGTTQYSTFSYVDPVITSISPKYGPMAGGTLLTLTGNYLNSGNSRHISIGGKTCTLKSVSNSILECYTPAQTISTEFAVKLKIDLANRETSIFSYREDPIVYEIHPTKSFISGGSTITGVGKNLNSVSVPRMVINVHEAGRNFTVACQHRSNSEIICCTTPSLQQLNLQLPLKTKAFFMLDGILSKYFDLIYVHNPVFKPFEKPVMISMGNENVLEIKGNDIDPEAVKGEVLKVGNKSCENIHLHSEAVLCTVPNDLLKLNSELNIEWKQAISSTVLGKVIVQPDQNFTASGAAWSHPQFEKGAAWSHPQFEKGAAWSHPQFEKGAAHHHHHH 976 AA PAT REGION 1..976 note Decoy Met mutation K842E-TAGs source 1..976 mol_type protein organism synthetic construct MKAPAVLAPGILVLLFTLVQRSNGECKEALAKSEMNVNMKYQLPNFTAETPIQNVILHEHHIFLGATNYIYVLNEEDLQKVAEYKTGPVLEHPDCFPCQDCSSKANLSGGVWKDNINMALVVDTYYDDQLISCGSVNRGTCQRHVFPHNHTADIQSEVHCIFSPQIEEPSQCPDCVVSALGAKVLSSVKDRFINFFVGNTINSSYFPDHPLHSISVRRLKETKDGFMFLTDQSYIDVLPEFRDSYPIKYVHAFESNNFIYFLTVQRETLDAQTFHTRIIRFCSINSGLHSYMEMPLECILTEKRKKRSTKKEVFNILQAAYVSKPGAQLARQIGASLNDDILFGVFAQSKPDSAEPMDRSAMCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARRDEYRTEFTTALQRVDLFMGQFSEVLLTSISTFIKGDLTIANLGTSEGRFMQVVVSRSGPSTPHVNFLLDSHPVSPEVIVEHTLNQNGYTLVITGKKITKIPLNGLGCRHFQSCSQCLSAPPFVQCGWCHDKCVRSEECLSGTWTQQICLPAIYKVFPNSAPLEGGTRLTICGWDFGFRRNNKFDLKKTRVLLGNESCTLTLSESTMNTLKCTVGPAMNKHFNMSIIISNGHGTTQYSTFSYVDPVITSISPKYGPMAGGTLLTLTGNYLNSGNSRHISIGGKTCTLKSVSNSILECYTPAQTISTEFAVKLKIDLANRETSIFSYREDPIVYEIHPTKSFISGGSTITGVGKNLNSVSVPRMVINVHEAGRNFTVACQHRSNSEIICCTTPSLQQLNLQLPLKTKAFFMLDGILSKYFDLIYVHNPVFEPFEKPVMISMGNENVLEIKGNDIDPEAVKGEVLKVGNKSCENIHLHSEAVLCTVPNDLLKLNSELNIEWKQAISSTVLGKVIVQPDQNFTASGAAWSHPQFEKGAAWSHPQFEKGAAWSHPQFEKGAAHHHHHH 131 AA PAT REGION 1..131 note murine VL source 1..131 mol_type protein organism synthetic construct METDTILLWVLLLWVPGSTGDIVLTQSPASLAVSLGQRATISCKASQSVDYDGGSYMSWFQQRPGQPPKLLISAASNLESGIPARFSGSGSGTDFTLNIHPVEEEDVATYYCQQSYEDPLTFGAGTKVEIK 131 AA PAT REGION 1..131 note murine VH source 1..131 mol_type protein organism synthetic construct MGWSYIILFLVATATDGHSQVQLQQPGTELVKPGASVKLSCKASGYTFTSYWIHWVKQRPGQGLEWIGEINPSSGRTNYNEKFKNKVTVTVDKSSTTAYMQLSNLTSEDSAVYYCASRGYWGQGTTLTVSS 131 AA PAT REGION 1..131 note humanized VL-cl03G05 source 1..131 mol_type protein organism synthetic construct METDTILLWVLLLWVPGSTGDIVLTQSPASLAVSLGERATISCKASQSVDYDGGSYMSWFQQRPGQPPKLLIYAASNLESGVPDRFSGSGSGTDFTLNISPLEAEDVAVYYCQQSYEDPLTFGGGTKVEIK 131 AA PAT REGION 1..131 note humanized VH-cl03G05 source 1..131 mol_type protein organism synthetic construct MGWSYIILFLVATATDGHSQVQLVQPGTEVKKPGASVKLSCKASGYTFTSYWIHWVRQAPGQGLEWMGEINPSSGRTNYNEKFKNRVTITADKSTSTAYMQLSSLRSEDTAVYYCASRGYWGQGTTVTVSS 131 AA PAT REGION 1..131 note humanized VL-cl03F04 source 1..131 mol_type protein organism synthetic construct METDTILLWVLLLWVPGSTGDIVMTQSPDSLAVSLGERATINCKASQSVDYDGGSYMSWYQQKPGQPPKLLISAASNLESGVPDRFSGSGSGTDFTLTIHSLQAEDVAVYYCQQSYEDPLTFGAGTKVEIK 131 AA PAT REGION 1..131 note humanized VH-cl03F04 source 1..131 mol_type protein organism synthetic construct MGWSYIILFLVATATDGHSQVQLQQPGAEVKKPGSSVKLSCKASGYTFTSYWIHWVKQAPGQGLEWIGEINPSSGRTNYNEKFKNKVTITVDKSTSTAYMELSNLRSEDSAVYYCASRGYWGQGTTLTVSS 131 AA PAT REGION 1..131 note humanized VL-cl03H08 source 1..131 mol_type protein organism synthetic construct METDTILLWVLLLWVPGSTGDIVLTQSPASLAVSLGERATISCKASQSVDYDGGSYMSWYQQKPGQPPKLLIYAASNLESGVPDRFSGSGSGTDFTLNISSVQEEDVATYYCQQSYEDPLTFGAGTKVEIK 131 AA PAT REGION 1..131 note humanized VH-cl03H08 source 1..131 mol_type protein organism synthetic construct MGWSYIILFLVATATDGHSQVQLVQSGAEVKKPGSSVKLSCKASGYTFSSYWIHWVRQAPGQGLEWMGEINPSSGRTNYNEKFKNRVTITADKSTSTAYMELSNLRSEDSAVYYCASRGYWGQGTTLTVSS 131 AA PAT REGION 1..131 note humanized VL-cl04H08 source 1..131 mol_type protein organism synthetic construct METDTILLWVLLLWVPGSTGDIVLTQSPASLAVSLGERATINCKASQSVDYDGGSYMSWYQQRPGQPPKLLIYAASNLESGVPDRFSGSGSGTDFTLTIHSLQAEDVATYYCQQSYEDPLTFGGGTKVEIK 131 AA PAT REGION 1..131 note humanized VH-cl04H08 source 1..131 mol_type protein organism synthetic construct MGWSYIILFLVATATDGHSQVQLQQPGAEVKKPGSSVKLSCKASGYTFTSYWIHWVKQAPGQGLEWIGEINPSSGRTNYNEKFKNKVTITVDKSTSTAYMELSSLRSEDTAVYYCASRGYWGQGTTVTVSS 717 DNA PAT misc_feature 1..717 note humanized VL+CL-cl03E08 (nucleotides) source 1..717 mol_type other DNA organism synthetic construct atggagacagacacaatcctgctatgggtgctgctgctctgggttccaggctccactggtgatattgtgctgacccagagcccggacagcctggcagtgagtctgggtcaacgcgccaccattaactgcaaagccagccagagcgtggattatgacggcggtagctacatgagctggtttcagcagaaaccgggtcagccgccgaaactgctgatttatgccgccagcaatctggaaagcggcgttccggcccgttttagcggtagcggcagcggcaccgattttaccctgaccattagctcgctgcaggcggaggatgtggccacctattactgccagcagagctacgaagatccgctgacctttggcggcggtaccaaagtggagattaaacgaactgtggctgcaccatctgtcttcatcttcccgccatctgatgagcagttgaaatctggaactgcctctgttgtgtgcctgctgaataacttctatcccagagaggccaaagtacagtggaaggtggataacgccctccaatcgggtaactcccaggagagtgtcacagagcaggacagcaaggacagcacctacagcctcagcagcaccctgacgctgagcaaagcagactacgagaaacacaaagtctacgcctgcgaagtcacccatcagggcctgagctcgcccgtcacaaagagcttcaacaggggagagtgttaa 717 DNA PAT misc_feature 1..717 note humanized VL+CL-cl03G05 (nucleotides) source 1..717 mol_type other DNA organism synthetic construct atggagacagacacaatcctgctatgggtgctgctgctctgggttccaggctccactggtgacatcgtgctgacccagtcccctgcttccctcgctgtgtccctgggcgagagggctaccatctcctgtaaggcctcccagtccgtggactacgacggcggctcctacatgtcctggttccagcagaggcctggccagcctcccaagctgctgatctacgctgcctccaacctggagtccggcgtgcctgataggttcagcggatccggctccggcaccgacttcaccctgaatatctcccctctggaggctgaggacgtggccgtgtactactgccagcagtcctacgaggaccctctgaccttcggcggcggcaccaaggtggagatcaagagaactgtcgcagcaccttccgtctttattttcccaccatcagatgaacagctgaaatccggtactgccagtgtggtctgtctgctcaataatttctatccccgggaggctaaagtccagtggaaagtggataatgcccttcagtccggcaactcccaggagagcgtgaccgagcaggattctaaggactcaacatattcactgtcatctactctcaccctgtctaaggctgattacgaaaagcacaaggtgtacgcttgcgaagttactcatcagggcctctcttcccctgtgaccaagagtttcaaccggggcgagtgctaa 717 DNA PAT misc_feature 1..717 note humanized VL+CL-cl03F04 (nucleotides) source 1..717 mol_type other DNA organism synthetic construct atggagacagacacaatcctgctatgggtgctgctgctctgggttccaggctccactggtgacatcgtgatgacccagtcccctgactccctggctgtgtccctgggcgaaagggccaccatcaactgcaaggcctcccagtccgtggactacgacggcggctcctacatgtcctggtaccagcagaagcccggccagcctcccaagctgctgatctccgccgcttccaatctggagtccggcgtgcccgataggttttccggctccggctccggcaccgactttaccctgaccatccactccctgcaggccgaggacgtggctgtgtactactgccagcagtcctacgaggaccctctgaccttcggcgccggcaccaaggtggagatcaagagaactgtcgcagcaccttccgtctttattttcccaccatcagatgaacagctgaaatccggtactgccagtgtggtctgtctgctcaataatttctatccccgggaggctaaagtccagtggaaagtggataatgcccttcagtccggcaactcccaggagagcgtgaccgagcaggattctaaggactcaacatattcactgtcatctactctcaccctgtctaaggctgattacgaaaagcacaaggtgtacgcttgcgaagttactcatcagggcctctcttcccctgtgaccaagagtttcaaccggggcgagtgctaa 717 DNA PAT misc_feature 1..717 note humanized VL+CL-cl03H08 (nucleotides) source 1..717 mol_type other DNA organism synthetic construct atggagacagacacaatcctgctatgggtgctgctgctctgggttccaggctccactggtgacatcgtgctgacccagtcccctgcttccctggctgtgtccctgggcgagagggctaccatctcctgcaaggcctcccagtccgtggactacgacggcggctcctacatgtcctggtaccagcagaagcccggccagcctcccaagctgctgatctacgccgcctccaacctggagtccggcgtgcccgataggttctccggatccggctccggcaccgacttcaccctgaacatctcctccgtgcaggaggaggacgtggccacctactactgccagcagtcctatgaggaccccctgaccttcggcgctggcaccaaggtggagatcaagagaactgtcgcagcaccttccgtctttattttcccaccatcagatgaacagctgaaatccggtactgccagtgtggtctgtctgctcaataatttctatccccgggaggctaaagtccagtggaaagtggataatgcccttcagtccggcaactcccaggagagcgtgaccgagcaggattctaaggactcaacatattcactgtcatctactctcaccctgtctaaggctgattacgaaaagcacaaggtgtacgcttgcgaagttactcatcagggcctctcttcccctgtgaccaagagtttcaaccggggcgagtgctaa 717 DNA PAT misc_feature 1..717 note humanized VL+CL-cl04H08 (nucleotides) source 1..717 mol_type other DNA organism synthetic construct atggagacagacacaatcctgctatgggtgctgctgctctgggttccaggctccactggtgacatcgtgctgacccagtcccctgcttccctggctgtgtccctgggagagagggccaccatcaactgcaaggcctcccagtccgtggactacgacggcggctcctacatgtcctggtaccagcagaggcccggacagcctcccaagctgctgatctacgctgcctccaacctggagtccggcgtgcctgataggttttccggctccggctccggaaccgacttcaccctgaccatccactccctgcaggcggaggacgtggccacctactactgccagcagtcctacgaggaccccctgacattcggcggcggaaccaaggtggagatcaagagaactgtcgcagcaccttccgtctttattttcccaccatcagatgaacagctgaaatccggtactgccagtgtggtctgtctgctcaataatttctatccccgggaggctaaagtccagtggaaagtggataatgcccttcagtccggcaactcccaggagagcgtgaccgagcaggattctaaggactcaacatattcactgtcatctactctcaccctgtctaaggctgattacgaaaagcacaaggtgtacgcttgcgaagttactcatcagggcctctcttcccctgtgaccaagagtttcaaccggggcgagtgctaa 717 DNA PAT misc_feature 1..717 note chimeric VL+CL (nucleotides) source 1..717 mol_type other DNA organism synthetic construct atggagacagacacaatcctgctatgggtgctgctgctctgggttccaggctccactggtgacattgtgctgacccaatctccagcttctttggctgtgtctctagggcagagggccaccatctcctgcaaggccagccaaagtgttgattatgatggtggtagttatatgagttggttccaacagagaccaggacagccacccaaactcctcatctctgctgcatccaaccttgaatctggcatcccagccaggtttagtggcagtggctctgggacagacttcaccctcaatatccatcctgtggaggaggaggatgttgcaacctattactgtcagcaaagttatgaagacccgctcacgttcggtgctggtaccaaggtggagatcaaacgaactgtggctgcaccatctgtcttcatcttcccgccatctgatgagcagttgaaatctggaactgcctctgttgtgtgcctgctgaataacttctatcccagagaggccaaagtacagtggaaggtggataacgccctccaatcgggtaactcccaggagagtgtcacagagcaggacagcaaggacagcacctacagcctcagcagcaccctgacgctgagcaaagcagactacgagaaacacaaagtctacgcctgcgaagtcacccatcagggcctgagctcgcccgtcacaaagagcttcaacaggggagagtgttaa 1386 DNA PAT misc_feature 1..1386 note humanized VH+CH1+CH2+CH3-cl03E08, knob mutation, (nucleotides) source 1..1386 mol_type other DNA organism synthetic construct atgggatggagctatatcatcctctttttggtagcaacagctacagatggccactcccaggtgcagctgcagcagtcgggtgccgaagtgaaaaaaccgggtgctagtgtgaaactgagctgcaaagccagcggctataccttcaccagctactggatccactgggtgcgtcaggcgcctggccagggcctggaatggattggcgaaatcaatccgagcagcggtcgcaccaattacaatgaaaaatttaaaaatcgtgtgaccgttaccgtggataaaagcacctccaccgcctatatggagctgagcagcctgaccagcgaagatagcgccgtgtattattgcgccagccgcggttattggggccagggtaccaccctgaccgtgagcagcgctagcacgaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcaggcgccctgaccagcggcgtgcacaccttcccggctgtcctacagtcctcaggactctactccctcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacctacatctgcaacgtgaatcacaagcccagcaacaccaaggtggacaagaaagttgagcccaaatcttgtgacaaaactcacacatgcccaccgtgcccagcacctgaactcctggggggaccgtcagtcttcctcttccccccaaaacccaaggacaccctcatgatctcccggacccctgaggtcacatgcgtggtggtggacgtgagccacgaagaccctgaggtcaagttcaactggtacgtggacggcgtggaggtgcataatgccaagacaaagccgcgggaggagcagtacaacagcacgtaccgtgtggtcagcgtcctcaccgtcctgcaccaggactggctgaatggcaaggagtacaagtgcaaggtctccaacaaagccctcccagcccccatcgagaaaaccatctccaaagccaaagggcagccccgagaaccacaggtgtacaccctgcccccatcccgggatgagctgaccaagaaccaggtcagcctgtggtgcctggtcaaaggcttctatcccagcgacatcgccgtggagtgggagagcaatgggcagccggagaacaactacaagaccacgcctcccgtgctggactccgacggctccttcttcctctacagcaagctcaccgtggacaagagcaggtggcagcaggggaacgtcttctcatgctccgtgatgcatgaggctctgcacaaccactacacgcagaagagcctctccctgtctccgggtaaatag 1386 DNA PAT misc_feature 1..1386 note humanized VH+CH1+CH2+CH3-cl03G05, knob mutation, (nucleotides) source 1..1386 mol_type other DNA organism synthetic construct atgggatggagctatatcatcctctttttggtagcaacagctacagatggccactcccaggtgcagctggtgcagcccggcacagaggtgaagaagcctggcgcctccgtgaagctgtcctgcaaggcctccggctacaccttcaccagctactggatccactgggtgaggcaggctcctggacagggcctggagtggatgggcgagatcaacccctcctccggcaggaccaactacaacgagaagttcaagaaccgggtgaccatcaccgccgacaagtccacctccaccgcctacatgcagctgtcctccctgaggtccgaggacaccgccgtgtactactgcgcctccaggggatattggggccagggcaccacagtgaccgtgagctccgctagcacgaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcaggcgccctgaccagcggcgtgcacaccttcccggctgtcctacagtcctcaggactctactccctcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacctacatctgcaacgtgaatcacaagcccagcaacaccaaggtggacaagaaagttgagcccaaatcttgtgacaaaactcacacatgcccaccgtgcccagcacctgaactcctggggggaccgtcagtcttcctcttccccccaaaacccaaggacaccctcatgatctcccggacccctgaggtcacatgcgtggtggtggacgtgagccacgaagaccctgaggtcaagttcaactggtacgtggacggcgtggaggtgcataatgccaagacaaagccgcgggaggagcagtacaacagcacgtaccgtgtggtcagcgtcctcaccgtcctgcaccaggactggctgaatggcaaggagtacaagtgcaaggtctccaacaaagccctcccagcccccatcgagaaaaccatctccaaagccaaagggcagccccgagaaccacaggtgtacaccctgcccccatcccgggatgagctgaccaagaaccaggtcagcctgtggtgcctggtcaaaggcttctatcccagcgacatcgccgtggagtgggagagcaatgggcagccggagaacaactacaagaccacgcctcccgtgctggactccgacggctccttcttcctctacagcaagctcaccgtggacaagagcaggtggcagcaggggaacgtcttctcatgctccgtgatgcatgaggctctgcacaaccactacacgcagaagagcctctccctgtctccgggtaaatag 1386 DNA PAT misc_feature 1..1386 note humanized VH+CH1+CH2+CH3-cl03F04, knob mutation (nucleotides) source 1..1386 mol_type other DNA organism synthetic construct atgggatggagctatatcatcctctttttggtagcaacagctacagatggccactcccaggtgcagctgcagcagcccggagccgaagtgaagaagcccggctccagcgtgaagctgtcctgcaaggcctccggctacaccttcacctcctactggatccactgggtgaagcaggctcctggacagggcctggagtggatcggcgagatcaacccctcctccggccggaccaactacaacgagaagttcaagaacaaggtgaccatcaccgtggacaagtccacctccaccgcctacatggagctgtccaacctgcggtccgaggactccgccgtgtactactgtgcctccaggggctattggggccagggcacaaccctgaccgtgtcctccgctagcacgaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcaggcgccctgaccagcggcgtgcacaccttcccggctgtcctacagtcctcaggactctactccctcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacctacatctgcaacgtgaatcacaagcccagcaacaccaaggtggacaagaaagttgagcccaaatcttgtgacaaaactcacacatgcccaccgtgcccagcacctgaactcctggggggaccgtcagtcttcctcttccccccaaaacccaaggacaccctcatgatctcccggacccctgaggtcacatgcgtggtggtggacgtgagccacgaagaccctgaggtcaagttcaactggtacgtggacggcgtggaggtgcataatgccaagacaaagccgcgggaggagcagtacaacagcacgtaccgtgtggtcagcgtcctcaccgtcctgcaccaggactggctgaatggcaaggagtacaagtgcaaggtctccaacaaagccctcccagcccccatcgagaaaaccatctccaaagccaaagggcagccccgagaaccacaggtgtacaccctgcccccatcccgggatgagctgaccaagaaccaggtcagcctgtggtgcctggtcaaaggcttctatcccagcgacatcgccgtggagtgggagagcaatgggcagccggagaacaactacaagaccacgcctcccgtgctggactccgacggctccttcttcctctacagcaagctcaccgtggacaagagcaggtggcagcaggggaacgtcttctcatgctccgtgatgcatgaggctctgcacaaccactacacgcagaagagcctctccctgtctccgggtaaatag 1386 DNA PAT misc_feature 1..1386 note humanized VH+CH1+CH2+CH3-cl03H08, knob mutation, (nucleotides) source 1..1386 mol_type other DNA organism synthetic construct atgggatggagctatatcatcctctttttggtagcaacagctacagatggccactcccaggtgcagctggtgcagtccggcgccgaagtgaagaagcccggctcctccgtgaagctgagctgcaaggcctccggctacaccttctcctcctactggatccactgggtgaggcaggctcctggacagggcctggagtggatgggcgagatcaacccctcctccggcaggaccaactacaacgagaagttcaagaaccgggtgaccatcaccgccgacaagtccacctccaccgcctacatggagctgtccaacctgaggtccgaggactccgccgtgtactactgcgcctccaggggatattggggccagggcaccaccctgacagtgtcctccgctagcacgaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcaggcgccctgaccagcggcgtgcacaccttcccggctgtcctacagtcctcaggactctactccctcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacctacatctgcaacgtgaatcacaagcccagcaacaccaaggtggacaagaaagttgagcccaaatcttgtgacaaaactcacacatgcccaccgtgcccagcacctgaactcctggggggaccgtcagtcttcctcttccccccaaaacccaaggacaccctcatgatctcccggacccctgaggtcacatgcgtggtggtggacgtgagccacgaagaccctgaggtcaagttcaactggtacgtggacggcgtggaggtgcataatgccaagacaaagccgcgggaggagcagtacaacagcacgtaccgtgtggtcagcgtcctcaccgtcctgcaccaggactggctgaatggcaaggagtacaagtgcaaggtctccaacaaagccctcccagcccccatcgagaaaaccatctccaaagccaaagggcagccccgagaaccacaggtgtacaccctgcccccatcccgggatgagctgaccaagaaccaggtcagcctgtggtgcctggtcaaaggcttctatcccagcgacatcgccgtggagtgggagagcaatgggcagccggagaacaactacaagaccacgcctcccgtgctggactccgacggctccttcttcctctacagcaagctcaccgtggacaagagcaggtggcagcaggggaacgtcttctcatgctccgtgatgcatgaggctctgcacaaccactacacgcagaagagcctctccctgtctccgggtaaatag 1386 DNA PAT misc_feature 1..1386 note humanized VH+CH1+CH2+CH3-cl04H08, knob mutation, (nucleotides) source 1..1386 mol_type other DNA organism synthetic construct atgggatggagctatatcatcctctttttggtagcaacagctacagatggccactcccaggtgcagctgcagcagcctggagccgaggtgaagaagcccggctcctccgtgaagctgtcctgcaaggcctccggctacaccttcacctcctactggatccactgggtgaagcaggcccctggacagggcctggagtggatcggcgagatcaacccctcctccggcaggaccaactacaacgagaagttcaagaacaaggtgaccatcaccgtggacaagtccacctccaccgcctacatggagctgtcctccctgaggtccgaggacaccgccgtgtactactgtgcctccaggggctattggggccagggcacaaccgtgaccgtgtcctccgctagcacgaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcaggcgccctgaccagcggcgtgcacaccttcccggctgtcctacagtcctcaggactctactccctcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacctacatctgcaacgtgaatcacaagcccagcaacaccaaggtggacaagaaagttgagcccaaatcttgtgacaaaactcacacatgcccaccgtgcccagcacctgaactcctggggggaccgtcagtcttcctcttccccccaaaacccaaggacaccctcatgatctcccggacccctgaggtcacatgcgtggtggtggacgtgagccacgaagaccctgaggtcaagttcaactggtacgtggacggcgtggaggtgcataatgccaagacaaagccgcgggaggagcagtacaacagcacgtaccgtgtggtcagcgtcctcaccgtcctgcaccaggactggctgaatggcaaggagtacaagtgcaaggtctccaacaaagccctcccagcccccatcgagaaaaccatctccaaagccaaagggcagccccgagaaccacaggtgtacaccctgcccccatcccgggatgagctgaccaagaaccaggtcagcctgtggtgcctggtcaaaggcttctatcccagcgacatcgccgtggagtgggagagcaatgggcagccggagaacaactacaagaccacgcctcccgtgctggactccgacggctccttcttcctctacagcaagctcaccgtggacaagagcaggtggcagcaggggaacgtcttctcatgctccgtgatgcatgaggctctgcacaaccactacacgcagaagagcctctccctgtctccgggtaaatag 1386 DNA PAT misc_feature 1..1386 note chimeric VH+CH1+CH2+CH3, knob mutation, (nucleotides) source 1..1386 mol_type other DNA organism synthetic construct atgggatggagctatatcatcctctttttggtagcaacagctacagatggccactcccaggtccaactgcaacagcctgggactgaactggtgaagcctggggcttcagtgaagctgtcctgcaaggcttctggctacaccttcaccagttactggatacactgggtgaagcagaggcctggacaaggccttgagtggattggagagattaatcctagcagcggtcgtactaactacaacgagaaattcaagaacaaggtcacagtgactgtagacaaatcttccaccacagcctacatgcaactcagcaacctgacatctgaggactctgcggtctattactgtgcaagtaggggctactggggccaaggcaccactctcacagtctcctcagctagcacgaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcaggcgccctgaccagcggcgtgcacaccttcccggctgtcctacagtcctcaggactctactccctcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacctacatctgcaacgtgaatcacaagcccagcaacaccaaggtggacaagaaagttgagcccaaatcttgtgacaaaactcacacatgcccaccgtgcccagcacctgaactcctggggggaccgtcagtcttcctcttccccccaaaacccaaggacaccctcatgatctcccggacccctgaggtcacatgcgtggtggtggacgtgagccacgaagaccctgaggtcaagttcaactggtacgtggacggcgtggaggtgcataatgccaagacaaagccgcgggaggagcagtacaacagcacgtaccgtgtggtcagcgtcctcaccgtcctgcaccaggactggctgaatggcaaggagtacaagtgcaaggtctccaacaaagccctcccagcccccatcgagaaaaccatctccaaagccaaagggcagccccgagaaccacaggtgtacaccctgcccccatcccgggatgagctgaccaagaaccaggtcagcctgtggtgcctggtcaaaggcttctatcccagcgacatcgccgtggagtgggagagcaatgggcagccggagaacaactacaagaccacgcctcccgtgctggactccgacggctccttcttcctctacagcaagctcaccgtggacaagagcaggtggcagcaggggaacgtcttctcatgctccgtgatgcatgaggctctgcacaaccactacacgcagaagagcctctccctgtctccgggtaaatag 741 DNA PAT misc_feature 1..741 note human CH2+CH3, Hole mutations, (nucleotides) source 1..741 mol_type other DNA organism synthetic construct atgggatggagctatatcatcctctttttggtagcaacagctacagatggccactccgacaaaactcacacatgcccaccgtgcccagcacctgaactcctggggggaccgtcagtcttcctcttccccccaaaacccaaggacaccctcatgatctcccggacccctgaggtcacatgcgtggtggtggacgtgagccacgaagaccctgaggtcaagttcaactggtacgtggacggcgtggaggtgcataatgccaagacaaagccgcgggaggagcagtacaacagcacgtaccgtgtggtcagcgtcctcaccgtcctgcaccaggactggctgaatggcaaggagtacaagtgcaaggtctccaacaaagccctcccagcccccatcgagaaaaccatctccaaagccaaagggcagccccgagaaccacaggtgtacaccctgcccccatcccgggatgagctgaccaagaaccaggtcagcctgagttgcgcggtcaaaggcttctatcccagcgacatcgccgtggagtgggagagcaatgggcagccggagaacaactacaagaccacgcctcccgtgctggactccgacggctccttcttcctcgtcagcaagctcaccgtggacaagagcaggtggcagcaggggaacgtcttctcatgctccgtgatgcatgaggctctgcacaaccactacacgcagaagagcctctccctgtctccgggtaaatag 18 DNA PAT misc_feature 1..18 note His-TAG (nucleotide) source 1..18 mol_type other DNA organism synthetic construct caccatcaccatcaccat 6 AA PAT REGION 1..6 note His-TAG source 1..6 mol_type protein organism synthetic construct HHHHHH 108 DNA PAT misc_feature 1..108 note Strep-TAG (nucleotide) source 1..108 mol_type other DNA organism synthetic construct ggtgccgcatggagccacccccagttcgaaaaaggggccgcatggagccacccccagttcgaaaaaggggccgcatggagccacccccagttcgaaaaaggggccgca 36 AA PAT REGION 1..36 note Strep-TAG source 1..36 mol_type protein organism synthetic construct GAAWSHPQFEKGAAWSHPQFEKGAAWSHPQFEKGAA 39 DNA PAT misc_feature 1..39 note K842E sense nucleotide source 1..39 mol_type other DNA organism synthetic construct gtacataatcctgtgtttgagccttttgaaaagccagtg 39 DNA PAT misc_feature 1..39 note K842E antisense nucleotide source 1..39 mol_type other DNA organism synthetic construct cactggcttttcaaaaggctcaaacacaggattatgtac