01183-0210-00PCT-KAD US US 63/284,937 2021-12-01 KADMON CORPORATION, LLC LU, Dan et al. B7-H4 ANTIBODIES AND ANTI-B7-H4 ANTIBODY/IL-15 FUSION PROTEINS 189 117 AA PAT REGION 1..117 note Synthetic: 1A12 VH source 1..117 mol_type protein organism synthetic construct EVQLLESGGGLVKPGGSLRLSCAASGFTFSSYAMHWVRQAPGKGLEWVAVISYDGSNKYYADSVKGRFTISRDDSKNTLYLQMNSLKTEDTAVYYCARHGWDAFDIWGQGTLVTVSS 107 AA PAT REGION 1..107 note Synthetic: 1A12 VL source 1..107 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQDISNRLNWYQQKPGKAPKLLIYLASSLQGGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQAISFPLTFGGGTKVEIK 121 AA PAT REGION 1..121 note Synthetic: 1D3 VH source 1..121 mol_type protein organism synthetic construct EVQLLESGGGLVQPGGSLRLSCAASGFTFNTHAMHWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDISKNTLYLQMNSLRAEDTAVYYCARLLGRFGEYGMDVWGQGTTVTVSS 10 AA PAT REGION 1..10 note Synthetic: 1D3 CDR1H source 1..10 mol_type protein organism synthetic construct GFTFNTHAMH 17 AA PAT REGION 1..17 note Synthetic: 1D3 CDR2H source 1..17 mol_type protein organism synthetic construct AISGSGGSTYYADSVKG 12 AA PAT REGION 1..12 note Synthetic: 1D3 CDR3H source 1..12 mol_type protein organism synthetic construct LLGRFGEYGMDV 107 AA PAT REGION 1..107 note Synthetic: 1D3 VL source 1..107 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASRSIYTWLAWYQQKPGKAPKLLIYDASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPYTFGGGTKVEIK 11 AA PAT REGION 1..11 note Synthetic: 1D3 CDR1L source 1..11 mol_type protein organism synthetic construct RASRSIYTWLA 5 AA PAT REGION 1..5 note Synthetic: 1D3 CDR2L source 1..5 mol_type protein organism synthetic construct STLQS 9 AA PAT REGION 1..9 note Synthetic: 1D3 CDR3L source 1..9 mol_type protein organism synthetic construct QQSYSTPYT 116 AA PAT REGION 1..116 note Synthetic: 1F11 VH source 1..116 mol_type protein organism synthetic construct QVQLVQSGAEVKKPGSSVKVSCKASGDTFTTYQIHWVRQAPGQGLEWMGGIMPIFGTTKYAQNFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARRNYGMDVWGQGTTVTVSS 113 AA PAT REGION 1..113 note Synthetic: 1F11 VL source 1..113 mol_type protein organism synthetic construct DIVMTQSPDSLAVSLGERATINCKSSQSLLYRPVNKNFLAWYQQKPGQPPKLLIYWASTRGSGVPDRFSGSGSGTDFTLTISSLQAEDVATYFCQQYYSTPITFGQGTKVEIK 116 AA PAT REGION 1..116 note Synthetic: 3D1 VH source 1..116 mol_type protein organism synthetic construct QVQLVQSGAEVKKPGSSVKVSCKASGYSFISYAITWVRQAPGQGLEWIGGINPIFGTAKYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCAKGQALIQHWGQGTLVTVSS 113 AA PAT REGION 1..113 note Synthetic: 3D1 VL source 1..113 mol_type protein organism synthetic construct DIVMTQSPDSLAVSLGERATINCKSSQSVLSSSNNKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSTPLAFGQGTKVEIK 116 AA PAT REGION 1..116 note Synthetic: 3F2 VH source 1..116 mol_type protein organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTAQYMYWVRQAPGQGLEWMGRINPTSGNTVYAQKLQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGINWFDPWGQGTLVTVSS 10 AA PAT REGION 1..10 note Synthetic: 3F2 CDR1H source 1..10 mol_type protein organism synthetic construct GYTFTAQYMY 17 AA PAT REGION 1..17 note Synthetic: 3F2 CDR2H source 1..17 mol_type protein organism synthetic construct RINPTSGNTVYAQKLQG 7 AA PAT REGION 1..7 note Synthetic: 3F2 CDR3H source 1..7 mol_type protein organism synthetic construct GINWFDP 107 AA PAT REGION 1..107 note Synthetic: 3F2 VL source 1..107 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTFGGGTKVEIK 11 AA PAT REGION 1..11 note Synthetic: 3F2 CDR1L source 1..11 mol_type protein organism synthetic construct RASQSISSYLN 5 AA PAT REGION 1..5 note Synthetic: 3F2 CDR2L source 1..5 mol_type protein organism synthetic construct SSLQS 9 AA PAT REGION 1..9 note Synthetic: 3F2 CDR3L source 1..9 mol_type protein organism synthetic construct QQSYSTPLT 118 AA PAT REGION 1..118 note Synthetic: 4H6 VH source 1..118 mol_type protein organism synthetic construct EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYGMHWVRQAPGKGLEWVSGISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARTQWRASLDPWGQGTLVNVSS 107 AA PAT REGION 1..107 note Synthetic: 4H6 VL source 1..107 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQDISYWLAWYQQKPGKAPKLLIYGASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQTTMSPYTFGQGTKVEIK 120 AA PAT REGION 1..120 note Synthetic: 6C3 VH source 1..120 mol_type protein organism synthetic construct EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMHWVRQAPGKGLEWVASISGTGFTTRYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARGGWFRDTPVDYWGQGTLVTVSS 112 AA PAT REGION 1..112 note Synthetic: 6C3 VL source 1..112 mol_type protein organism synthetic construct DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQLLIYGASSLRSGVPDRFSGSGSGTDFTLKISRVEAZDVGVYYCMQASHWPPTFGQGTRLEIK 115 AA PAT REGION 1..115 note Synthetic: 9H2 VH source 1..115 mol_type protein organism synthetic construct QVQLVQSGAEVKKPGSSVKVSCKASGGTFSTYAINWVRQAPGQGLEWMGGINPMFGTARYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARGRLHRHWGQGTLVTVSS 113 AA PAT REGION 1..113 note Synthetic: 9H2 VL source 1..113 mol_type protein organism synthetic construct DIVMTQSPDSLAVSLGERATINCKSSQSVLYSSNNKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYTTPITFGQGTRLEIK 122 AA PAT REGION 1..122 note Synthetic: 4B9 VH source 1..122 mol_type protein organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYYMHWVRQAPGQGLEWMGWMNPNSGNTGYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGQGRYLNYYYMDVWGKGTTVTVSS 107 AA PAT REGION 1..107 note Synthetic: 4B9 VL source 1..107 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRVSQGISNYLAWYQQKPGKAPKLLIYDASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPFTFGPGTKVDIK 118 AA PAT REGION 1..118 note Synthetic: 15B11 VH source 1..118 mol_type protein organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYIHWVRQAPGQGLEWMGWMNPHSGHTGYAQNLQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARVKVTTGFDYWGQGTLVTVSS 112 AA PAT REGION 1..112 note Synthetic: 15B11 VL source 1..112 mol_type protein organism synthetic construct DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQLLIYLGSNRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQGTHWPPTFGQGTKLEIK 118 AA PAT REGION 1..118 note Synthetic: 24B6 VH source 1..118 mol_type protein organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYMHWVRQAPGQGLEWIGWMNPNSGDTGYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAKVGAYGGMDVWGQGTTVTVSS 112 AA PAT REGION 1..112 note Synthetic: 24B6 VL source 1..112 mol_type protein organism synthetic construct DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQLLIYGGSNRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQGTHWPPTFGPGTKVDIK 119 AA PAT REGION 1..119 note Synthetic: 24F4 VH source 1..119 mol_type protein organism synthetic construct QVQLVQSGAEVKKPGSSVKVSCKASGYRFTGDYIHWVRQAPGQGLEWMGWMNPNSGNTGLAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARVLGFSGGMDVWGQGTTVTVSS 112 AA PAT REGION 1..112 note Synthetic: 24F4 VL source 1..112 mol_type protein organism synthetic construct DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQLLIYAASSLRSGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQGTHWPPTFGQGTKLEIK 118 AA PAT REGION 1..118 note Synthetic: 30G4 VH source 1..118 mol_type protein organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFSSYYMHWVRQAPGQGLEWMGWMNPNSGDTGYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARVPETGGMDVWGQGTTVTVSS 112 AA PAT REGION 1..112 note Synthetic: 30G4 VL source 1..112 mol_type protein organism synthetic construct DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQLLIYGASSLHSGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQGTHWPPTFGQGTKVEIK 127 AA PAT REGION 1..127 note Synthetic: 5E4 VH source 1..127 mol_type protein organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYDINWVRQAPGQGLEWMGWMNPNSGNTGYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGELWFGEFDYYYYYGMDVWGQGTTVTVSS 107 AA PAT REGION 1..107 note Synthetic: 5E4 VL source 1..107 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYGASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQADRIPFTFGRGTKVEIK 120 AA PAT REGION 1..120 note Synthetic: 5F4 VH source 1..120 mol_type protein organism synthetic construct EVQLLESGGGLVKPGGSLRLSCAASGFTFSSYAMHWVRQAPGKGLEWVSAIGGSGRTTYYADSVKGRFTISRDDSKNTLYLQMNSLKTEDTAVYYCARERGYSYGQIDYWGQGTLVTVSS 112 AA PAT REGION 1..112 note Synthetic: 5F4 VL source 1..112 mol_type protein organism synthetic construct DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQLLIYAASSLQSGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQGAHWPLTFGQGTKVEIK 119 AA PAT REGION 1..119 note Synthetic: 5G6 VH source 1..119 mol_type protein organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYYMHWVRQAPGQGLEWLGWVNPNTGTTRFAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDANYYYGMDVWGQGTMVTVSS 114 AA PAT REGION 1..114 note Synthetic: 5G6 VL source 1..114 mol_type protein organism synthetic construct DIVMTQSPDSLAVSLGERATINCKSSQSVLYSSNNKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVATYYCQKYNSALPITFGQGTRLEIK 120 AA PAT REGION 1..120 note Synthetic: 9D11 VH source 1..120 mol_type protein organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYGISWVRQAPGQGLEWVGVINPNGGTTTYAQTFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARTGYSSGWAFDYWGRGTLVTVSS 10 AA PAT REGION 1..10 note Synthetic: 9D11 CDR1H source 1..10 mol_type protein organism synthetic construct GYTFTSYGIS 17 AA PAT REGION 1..17 note Synthetic: 9D11 CDR2H source 1..17 mol_type protein organism synthetic construct VINPNGGTTTYAQTFQG 11 AA PAT REGION 1..11 note Synthetic: 9D11 CDR3H source 1..11 mol_type protein organism synthetic construct TGYSSGWAFDY 112 AA PAT REGION 1..112 note Synthetic: 9D11 VL source 1..112 mol_type protein organism synthetic construct DIVMTQSPDSLAVSLGERATINCKSSQSVLYSSNNKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYFSTPSFGQGTKVEIK 17 AA PAT REGION 1..17 note Synthetic: 9D11 CDR1L source 1..17 mol_type protein organism synthetic construct KSSQSVLYSSNNKNYLA 5 AA PAT REGION 1..5 note Synthetic: 9D11 CDR2L source 1..5 mol_type protein organism synthetic construct STRES 8 AA PAT REGION 1..8 note Synthetic: 9D11 CDR3L source 1..8 mol_type protein organism synthetic construct QQYFSTPS 119 AA PAT REGION 1..119 note Synthetic: 9E1 VH source 1..119 mol_type protein organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYLHWVRQAPGQGLEWMGWINPNSGGTNSAQRFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARVRGYEGGMDVWGQGTTVTVSS 112 AA PAT REGION 1..112 note Synthetic: 9E1 VL source 1..112 mol_type protein organism synthetic construct DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQLLIYLGSNRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQGSHWPPTFGQGTRLEIK 118 AA PAT REGION 1..118 note Synthetic: 31D7 VH source 1..118 mol_type protein organism synthetic construct EVQLLESGGGLVQPGGSXRLSCAASGFTFSSYGMHWVRQAPGKGLEWVSSISSSSSYIYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARIGSGGSLDYWGQGTLVTVSS 107 AA PAT REGION 1..107 note Synthetic: 31D7 VL source 1..107 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQSISNWLAWYQQKPGKAPKLLIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYTIPITFGQGTRLEIK 116 AA PAT REGION 1..116 note Synthetic: 39A11 VH source 1..116 mol_type protein organism synthetic construct EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYGMHWVRQAPGKGLEWVASISSSSGYIYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARASSGMDVWGQGTTVTVSS 10 AA PAT REGION 1..10 note Synthetic: 39A11 CDR1H source 1..10 mol_type protein organism synthetic construct GFTFSSYGMH 17 AA PAT REGION 1..17 note Synthetic: 39A11 CDR2H source 1..17 mol_type protein organism synthetic construct SISSSSGYIYYADSVKG 7 AA PAT REGION 1..7 note Synthetic: 39A11 CDR3H source 1..7 mol_type protein organism synthetic construct ASSGMDV 107 AA PAT REGION 1..107 note Synthetic: 39A11 VL source 1..107 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQTIRSYLNWYQQKPGKAPKLLIYAASHLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYTTPYTFGQGTKLEIK 11 AA PAT REGION 1..11 note Synthetic: 39A11 CDR1L source 1..11 mol_type protein organism synthetic construct RASQTIRSYLN 5 AA PAT REGION 1..5 note Synthetic: 39A11 CDR2L source 1..5 mol_type protein organism synthetic construct SHLQS 9 AA PAT REGION 1..9 note Synthetic: 39A11 CDR3L source 1..9 mol_type protein organism synthetic construct QQSYTTPYT 106 AA PAT REGION 1..106 note Synthetic: 49A2 VL source 1..106 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQNIDTYVNWYQQKPGKAPKLLIYAASRLHTVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYTSPFTFGGGTKVEIK 11 AA PAT REGION 1..11 note Synthetic: 49A2 CDR1L source 1..11 mol_type protein organism synthetic construct RASQNIDTYVN 5 AA PAT REGION 1..5 note Synthetic: 49A2 CDR2L source 1..5 mol_type protein organism synthetic construct SRLHT 9 AA PAT REGION 1..9 note Synthetic: 49A2 CDR3L source 1..9 mol_type protein organism synthetic construct QQSYTSPFT 107 AA PAT REGION 1..107 note Synthetic: 50A10 VL source 1..107 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCQASQDISNYINWYQQKPGKAPKLLIYAASRLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYRSPFTFGQGTKLEIK 11 AA PAT REGION 1..11 note Synthetic: 50A10 CDR1L source 1..11 mol_type protein organism synthetic construct QASQDISNYIN 5 AA PAT REGION 1..5 note Synthetic: 50A10 CDR2L source 1..5 mol_type protein organism synthetic construct SRLQS 9 AA PAT REGION 1..9 note Synthetic: 50A10 CDR3L source 1..9 mol_type protein organism synthetic construct QQSYRSPFT 11 AA PAT REGION 1..11 note Synthetic: 3F2 CDR motif CDR1L VARIANT 1 note X is R or Q VARIANT 5 note X is D, N, or S VARIANT 7 note X is D or S VARIANT 8 note X is N, S, or T VARIANT 10 note X is I, L, or V source 1..11 mol_type protein organism synthetic construct XASQXIXXYXN 000 9 AA PAT REGION 1..9 note Synthetic: 3F2 CDR motif CDR3L VARIANT 5 note X is R, S, or T VARIANT 6 note X is S or T VARIANT 8 note X is F or L source 1..9 mol_type protein organism synthetic construct QQSYXXPXT 107 AA PAT REGION 1..107 note Synthetic: 45A2 VL source 1..107 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQNIYTWLAWYQQKPGKAPKLLIYDATNLPTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTRWTFGGGTKVEIK 11 AA PAT REGION 1..11 note Synthetic: 45A2 CDR1L source 1..11 mol_type protein organism synthetic construct RASQNIYTWLA 5 AA PAT REGION 1..5 note Synthetic: 45A2 CDR2L source 1..5 mol_type protein organism synthetic construct TNLPT 9 AA PAT REGION 1..9 note Synthetic: 45A2 CDR3L source 1..9 mol_type protein organism synthetic construct QQSYSTRWT 107 AA PAT REGION 1..107 note Synthetic: 47B2 VL source 1..107 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQTVYTWLAWYQQKPGKAPKLLIYDATNLATGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTSWTFGGGTKVEIK 11 AA PAT REGION 1..11 note Synthetic: 47B2 CDR1L source 1..11 mol_type protein organism synthetic construct RASQTVYTWLA 5 AA PAT REGION 1..5 note Synthetic: 47B2 CDR2L source 1..5 mol_type protein organism synthetic construct TNLAT 9 AA PAT REGION 1..9 note Synthetic: 47B2 CDR3L source 1..9 mol_type protein organism synthetic construct QQSYSTSWT 11 AA PAT REGION 1..11 note Synthetic: 1D3 CDR motif CDR1L VARIANT 4 note X is Q or R VARIANT 5 note X is N, S, or T VARIANT 6 note X is I or V source 1..11 mol_type protein organism synthetic construct RASXXXYTWLA 000 9 AA PAT REGION 1..9 note Synthetic: 1D3 CDR motif CDR3L VARIANT 7 note X is P, R, or S VARIANT 8 note X is W or Y source 1..9 mol_type protein organism synthetic construct QQSYSTXXT 112 AA PAT REGION 1..112 note Synthetic: 67E12 VL source 1..112 mol_type protein organism synthetic construct DIVMTQSPDSLAVSLGERATINCKSSQSVLYSSNNKNYLAWYQQKPGQPPKLLIYWASKRVSGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYFDSPTFGQGTKVEIK 17 AA PAT REGION 1..17 note Synthetic: 67E12 CDR1L source 1..17 mol_type protein organism synthetic construct KSSQSVLYSSNNKNYLA 5 AA PAT REGION 1..5 note Synthetic: 67E12 CDR2L source 1..5 mol_type protein organism synthetic construct SKRVS 8 AA PAT REGION 1..8 note Synthetic: 67E12 CDR3L source 1..8 mol_type protein organism synthetic construct QQYFDSPT 112 AA PAT REGION 1..112 note Synthetic: 67C6 VL source 1..112 mol_type protein organism synthetic construct DIVMTQSPDSLAVSLGERATINCKSSQSVLSSSNNKNYLAWYQQKPGQPPKLLIYWASTRQSGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSDPTFGQGTKVEIK 17 AA PAT REGION 1..17 note Synthetic: 67C6 CDR1L source 1..17 mol_type protein organism synthetic construct KSSQSVLSSSNNKNYLA 5 AA PAT REGION 1..5 note Synthetic: 67C6 CDR2L source 1..5 mol_type protein organism synthetic construct STRQS 8 AA PAT REGION 1..8 note Synthetic: 67C6 CDR3L source 1..8 mol_type protein organism synthetic construct QQYYSDPT 112 AA PAT REGION 1..112 note Synthetic: 67C3 VL source 1..112 mol_type protein organism synthetic construct DIVMTQSPDSLAVSLGERATINCKSSQSVLYSSNNKNYLAWYQQKPGQPPKLLIYWASTRASGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYDTPTFGQGTKVEIK 17 AA PAT REGION 1..17 note Synthetic: 67C3 CDR1L source 1..17 mol_type protein organism synthetic construct KSSQSVLYSSNNKNYLA 5 AA PAT REGION 1..5 note Synthetic: 67C3 CDR2L source 1..5 mol_type protein organism synthetic construct STRAS 8 AA PAT REGION 1..8 note Synthetic: 67C3 CDR3L source 1..8 mol_type protein organism synthetic construct QQYYDTPT 112 AA PAT REGION 1..112 note Synthetic: 67G3 VL source 1..112 mol_type protein organism synthetic construct DIVMTQSPDSLAVSLGERATINCKSSQSVLSSSNNKNYLAWYQQKPGQPPKLLIYWASTRQSGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYTSPTFGQGTKVEIK 17 AA PAT REGION 1..17 note Synthetic: 67G3 CDR1L source 1..17 mol_type protein organism synthetic construct KSSQSVLSSSNNKNYLA 5 AA PAT REGION 1..5 note Synthetic: 67G3 CDR2L source 1..5 mol_type protein organism synthetic construct STRQS 8 AA PAT REGION 1..8 note Synthetic: 67G3 CDR3L source 1..8 mol_type protein organism synthetic construct QQYYTSPT 112 AA PAT REGION 1..112 note Synthetic: 67H9 VL source 1..112 mol_type protein organism synthetic construct DIVMTQSPDSLAVSLGERATINCRSSQSVLYSSNNKNYLAWYQQKPGQPPKLLIYWASNRKSGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSAPTFGQGTKVEIK 17 AA PAT REGION 1..17 note Synthetic: 67H9 CDR1L source 1..17 mol_type protein organism synthetic construct RSSQSVLYSSNNKNYLA 5 AA PAT REGION 1..5 note Synthetic: 67H9 CDR2L source 1..5 mol_type protein organism synthetic construct SNRKS 8 AA PAT REGION 1..8 note Synthetic: 67H9 CDR3L source 1..8 mol_type protein organism synthetic construct QQYYSAPT 112 AA PAT REGION 1..112 note Synthetic: 68F5 VL source 1..112 mol_type protein organism synthetic construct DIVMTQSPDSLAVSLGERATINCKSSRSVLSRSNNKNYLAWYQQKPGQPPKLLIYWASTRQFGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYDTPTFGQGTKVEIK 17 AA PAT REGION 1..17 note Synthetic: 68F5 CDR1L source 1..17 mol_type protein organism synthetic construct KSSRSVLSRSNNKNYLA 5 AA PAT REGION 1..5 note Synthetic: 68F5 CDR2L source 1..5 mol_type protein organism synthetic construct STRQF 8 AA PAT REGION 1..8 note Synthetic: 68F5 CDR3L source 1..8 mol_type protein organism synthetic construct QQYYDTPT 17 AA PAT REGION 1..17 note Synthetic: 9D11 CDR motif CDR1L VARIANT 1 note X is K or R VARIANT 4 note X is Q or R VARIANT 8 note X is S or Y VARIANT 9 note X is R or S source 1..17 mol_type protein organism synthetic construct XSSXSVLXXSNNKNYLA 000 8 AA PAT REGION 1..8 note Synthetic: 9D11 CDR motif CDR3L VARIANT 4 note X is F or Y VARIANT 5 note X is D, S, or T VARIANT 6 note X is A, D, S, or T VARIANT 8 note X is S or T source 1..8 mol_type protein organism synthetic construct QQYXXXPX 107 AA PAT REGION 1..107 note Synthetic: 57H3 VL source 1..107 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCQASQDIRKYLNWYQQKPGKAPKLLIYAASTRESGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYYSLPLTFGQGTKLEIK 11 AA PAT REGION 1..11 note Synthetic: 57H3 CDR1L source 1..11 mol_type protein organism synthetic construct QASQDIRKYLN 5 AA PAT REGION 1..5 note Synthetic: 57H3 CDR2L source 1..5 mol_type protein organism synthetic construct STRES 9 AA PAT REGION 1..9 note Synthetic: 57H3 CDR3L source 1..9 mol_type protein organism synthetic construct QQYYSLPLT 107 AA PAT REGION 1..107 note Synthetic: 57G8 VL source 1..107 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQSIRSYLNWYQQKPGKAPKLLIYYASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYYSTPLTFGQGTKVEIK 11 AA PAT REGION 1..11 note Synthetic: 57G8 CDR1L source 1..11 mol_type protein organism synthetic construct RASQSIRSYLN 5 AA PAT REGION 1..5 note Synthetic: 57G8 CDR2L source 1..5 mol_type protein organism synthetic construct SSLQS 9 AA PAT REGION 1..9 note Synthetic: 57G8 CDR3L source 1..9 mol_type protein organism synthetic construct QQYYSTPLT 107 AA PAT REGION 1..107 note Synthetic: 56A9 VL source 1..107 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYAASIRESGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYYTTPLTFGQGTKLEIK 11 AA PAT REGION 1..11 note Synthetic: 56A9 CDR1L source 1..11 mol_type protein organism synthetic construct RASQSISSYLN 5 AA PAT REGION 1..5 note Synthetic: 56A9 CDR2L source 1..5 mol_type protein organism synthetic construct SIRES 9 AA PAT REGION 1..9 note Synthetic: 56A9 CDR3L source 1..9 mol_type protein organism synthetic construct QQYYTTPLT 107 AA PAT REGION 1..107 note Synthetic: 56H7 VL source 1..107 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYAASNLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYYTTPLTFGQGTKLEIK 11 AA PAT REGION 1..11 note Synthetic: 56H7 CDR1L source 1..11 mol_type protein organism synthetic construct RASQSISSYLN 5 AA PAT REGION 1..5 note Synthetic: 56H7 CDR2L source 1..5 mol_type protein organism synthetic construct SNLQS 9 AA PAT REGION 1..9 note Synthetic: 56H7 CDR3L source 1..9 mol_type protein organism synthetic construct QQYYTTPLT 107 AA PAT REGION 1..107 note Synthetic: 62F9 VL source 1..107 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYAASIRESGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYYSTPLTFGQGTKLEIK 11 AA PAT REGION 1..11 note Synthetic: 62F9 CDR1L source 1..11 mol_type protein organism synthetic construct RASQSVSSAVA 5 AA PAT REGION 1..5 note Synthetic: 62F9 CDR2L source 1..5 mol_type protein organism synthetic construct SIRES 9 AA PAT REGION 1..9 note Synthetic: 62F9 CDR3L source 1..9 mol_type protein organism synthetic construct QQYYSTPLT 000 000 9 AA PAT REGION 1..9 note Synthetic: 39A11 CDR motif CDR3L VARIANT 3 note X is S or Y VARIANT 5 note X is S or T VARIANT 6 note X is L or T VARIANT 8 note X is L or Y source 1..9 mol_type protein organism synthetic construct QQXYXXPXT 120 AA PAT REGION 1..120 note Synthetic: Control antibody 1 VH source 1..120 mol_type protein organism synthetic construct QLQLQESGPGLVKPSETLSLTCTVSGGSIKSGSYYWGWIRQPPGKGLEWIGNIYYSGSTYYNPSLRSRVTISVDTSKNQFSLKLSSVTAADTAVYYCAREGSYPNQFDPWGQGTLVTVSS 107 AA PAT REGION 1..107 note Synthetic: Control antibody 1 VL source 1..107 mol_type protein organism synthetic construct EIVMTQSPATLSVSPGERATLSCRASQSVSSNLAWYQQKPGQAPRLLIYGASTRATGIPARFSGSGSGTEFTLTISSLQSEDFAVYYCQQYHSFPFTFGGGTKVEIK 115 AA PAT REGION 1..115 note Synthetic: Control antibody 2 (antibody 6H3 described in US9,574,000) VH source 1..115 mol_type protein organism synthetic construct EVQLQQSGPVLVKPGTSVKMSCKASGYTFTDYYMNWVKQSHGKSLEWIGVINPYNGDTTYNQKFKGKATLTVDKSSSTAYMEVNSLTFEDSAVYYCARYPESTYWGQGTLVTVSA 112 AA PAT REGION 1..112 note Synthetic: Control antibody 2 (antibody 6H3 described in US9,574,000) VL source 1..112 mol_type protein organism synthetic construct DVVMTQTPLSLPVSLGDQASISCRSSQSLVHINGNTYLHWYLQKPGQSPKVLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYFCSQSTHVPLTFGAGTKLELK 115 AA PAT REGION 1..115 note Synthetic: DP47 VH source 1..115 mol_type protein organism synthetic construct EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKGSGFDYWGQGTLVTVSS 108 AA PAT REGION 1..108 note Synthetic: DP47 VL source 1..108 mol_type protein organism synthetic construct EIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPLTFGQGTKVEIK 214 AA PAT REGION 1..214 note Synthetic: 20502 VL source 1..214 mol_type protein organism synthetic construct EIVMTQSPATLSVSPGERATLSCRASQSVSSNLAWYQQKPGQAPRLLIYGASTRATGIPARFSGSGSGTEFTLTISSLQSEDFAVYYCQQYHSFPFTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC 214 AA PAT REGION 1..214 note Synthetic: 57G8 VL source 1..214 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQSIRSYLNWYQQKPGKAPKLLIYYASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYYSTPLTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC 214 AA PAT REGION 1..214 note Synthetic: 50A10 VL source 1..214 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCQASQDISNYINWYQQKPGKAPKLLIYAASRLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYRSPFTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC 215 AA PAT REGION 1..215 note Synthetic: DP47 VL source 1..215 mol_type protein organism synthetic construct EIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPLTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC 450 AA PAT REGION 1..450 note Synthetic: 20502 VH source 1..450 mol_type protein organism synthetic construct QLQLQESGPGLVKPSETLSLTCTVSGGSIKSGSYYWGWIRQPPGKGLEWIGNIYYSGSTYYNPSLRSRVTISVDTSKNQFSLKLSSVTAADTAVYYCAREGSYPNQFDPWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPCRDELTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 446 AA PAT REGION 1..446 note Synthetic: 39A11 VH source 1..446 mol_type protein organism synthetic construct EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYGMHWVRQAPGKGLEWVASISSSSGYIYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARASSGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPCRDELTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 446 AA PAT REGION 1..446 note Synthetic: 39A11 VH LS mutation source 1..446 mol_type protein organism synthetic construct EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYGMHWVRQAPGKGLEWVASISSSSGYIYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARASSGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPCRDELTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVLHEALHSHYTQKSLSLSPGK 446 AA PAT REGION 1..446 note Synthetic: 3F2 VH source 1..446 mol_type protein organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTAQYMYWVRQAPGQGLEWMGRINPTSGNTVYAQKLQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGINWFDPWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPCRDELTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 446 AA PAT REGION 1..446 note Synthetic: 3F2 VH LS mutation source 1..446 mol_type protein organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTAQYMYWVRQAPGQGLEWMGRINPTSGNTVYAQKLQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGINWFDPWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPCRDELTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVLHEALHSHYTQKSLSLSPGK 445 AA PAT REGION 1..445 note Synthetic: DP47 HC1 source 1..445 mol_type protein organism synthetic construct EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKGSGFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPCRDELTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 445 AA PAT REGION 1..445 note Synthetic: LS mutation source 1..445 mol_type protein organism synthetic construct EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKGSGFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPCRDELTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVLHEALHSHYTQKSLSLSPGK 659 AA PAT REGION 1..659 note Synthetic: 20502 VH IL-15R-alpha sushi domain Linker IL-15 source 1..659 mol_type protein organism synthetic construct QLQLQESGPGLVKPSETLSLTCTVSGGSIKSGSYYWGWIRQPPGKGLEWIGNIYYSGSTYYNPSLRSRVTISVDTSKNQFSLKLSSVTAADTAVYYCAREGSYPNQFDPWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVCTLPPSRDELTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGSCPPPMSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKCIRDPALVHQRPAPPSGGSGGGGSGGGSGGGGSLQNWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVESLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS 655 AA PAT REGION 1..655 note Synthetic: 39A11 VH IL-15R-alpha sushi domain Linker IL-15 source 1..655 mol_type protein organism synthetic construct EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYGMHWVRQAPGKGLEWVASISSSSGYIYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARASSGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVCTLPPSRDELTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGSCPPPMSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKCIRDPALVHQRPAPPSGGSGGGGSGGGSGGGGSLQNWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVESLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS 655 AA PAT REGION 1..655 note Synthetic: 39A11 VH LS mutation IL-15R-alpha sushi domain Linker IL-15 source 1..655 mol_type protein organism synthetic construct EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYGMHWVRQAPGKGLEWVASISSSSGYIYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARASSGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVCTLPPSRDELTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSKLTVDKSRWQQGNVFSCSVLHEALHSHYTQKSLSLSPGSCPPPMSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKCIRDPALVHQRPAPPSGGSGGGGSGGGSGGGGSLQNWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVESLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS 655 AA PAT REGION 1..655 note Synthetic: 3F2 VH IL-15R-alpha sushi domain Linker IL-15 source 1..655 mol_type protein organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTAQYMYWVRQAPGQGLEWMGRINPTSGNTVYAQKLQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGINWFDPWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVCTLPPSRDELTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGSCPPPMSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKCIRDPALVHQRPAPPSGGSGGGGSGGGSGGGGSLQNWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVESLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS 655 AA PAT REGION 1..655 note Synthetic: 3F2 VH LS mutation IL-15R-alpha sushi domain Linker IL-15 source 1..655 mol_type protein organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTAQYMYWVRQAPGQGLEWMGRINPTSGNTVYAQKLQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGINWFDPWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVCTLPPSRDELTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSKLTVDKSRWQQGNVFSCSVLHEALHSHYTQKSLSLSPGSCPPPMSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKCIRDPALVHQRPAPPSGGSGGGGSGGGSGGGGSLQNWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVESLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS 654 AA PAT REGION 1..654 note Synthetic: DP47 VH IL-15R-alpha sushi domain Linker IL-15 source 1..654 mol_type protein organism synthetic construct EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKGSGFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVCTLPPSRDELTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGSCPPPMSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKCIRDPALVHQRPAPPSGGSGGGGSGGGSGGGGSLQNWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVESLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS 654 AA PAT REGION 1..654 note Synthetic: LS mutation IL-15R-alpha sushi domain Linker IL-15 source 1..654 mol_type protein organism synthetic construct EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKGSGFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVCTLPPSRDELTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSKLTVDKSRWQQGNVFSCSVLHEALHSHYTQKSLSLSPGSCPPPMSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKCIRDPALVHQRPAPPSGGSGGGGSGGGSGGGGSLQNWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVESLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS 282 AA PAT REGION 1..282 note misc_feature - human B7-H4 (NCBI accession #: NP_078902) signal peptide IgV domain IgC domain transmembrane region source 1..282 mol_type protein organism Homo sapiens MASLGQILFWSIISIIIILAGAIALIIGFGISGRHSITVTTVASAGNIGEDGILSCTFEPDIKLSDIVIQWLKEGVLGLVHEFKEGKDELSEQDEMFRGRTAVFADQVIVGNASLRLKNVQLTDAGTYKCYIITSKGKGNANLEYKTGAFSMPEVNVDYNASSETLRCEAPRWFPQPTVVWASQVDQGANFSEVSNTSFELNSENVTMKVVSVLYNVTINNTYSCMIENDIAKATGDIKVTESEIKRRSHLQLLNSKASLCVSSFFAISWALLPLSPYLMLK 283 AA PAT REGION 1..283 note misc_feature - Murine B7-H4 (NCBI accession#: Q7TSP5, Phe29-Pro258) signal peptide IgV domain IgC domain transmembrane region source 1..283 mol_type protein organism Mus musculus MASLGQIIFWSIINIIIILAGAIALIIGFGISGKHFITVTTFTSAGNIGEDGTLSCTFEPDIKLNGIVIQWLKEGIKGLVHEFKEGKDDLSQQHEMFRGRTAVFADQVVVGNASLRLKNVQLTDAGTYTCYIRTSKGKGNANLEYKTGAFSMPEINVDYNASSESLRCEAPRWFPQPTVAWASQVDQGANFSEVSNTSFELNSENVTMKVVSVLYNVTINNTYSCMIENDIAKATGDIKVTDSEVKRRSQLQLLNSGPSPCVFSSAFVAGWALLSLSCCLMLR 330 AA PAT REGION 1..330 note misc_feature - IgG1 Fc (NCBI accession #: P01857) Pro100-Lys330 underlined source 1..330 mol_type protein organism Homo sapiens ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 162 AA PAT REGION 1..162 note misc_feature - Full-length human Il-15 (NCBI accession #: P40933) mature protein source 1..162 mol_type protein organism Homo sapiens MRISKPHLRSISIQCYLCLLLNSHFLTEAGIHVFILGCFSAGLPKTEANWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVENLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS 114 AA PAT REGION 1..114 note misc_feature - Human IL-15 (mature protein) source 1..114 mol_type protein organism Homo sapiens NWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVENLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS 114 AA PAT REGION 1..114 note misc_feature - Human IL-15 N65S (mature protein) source 1..114 mol_type protein organism Homo sapiens NWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVESLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS 61 AA PAT REGION 1..61 note misc_feature - IL-15R-alpha sushi domain (residues 33-93 of NCBI accession #: EAW86418.1) source 1..61 mol_type protein organism Homo sapiens CPPPMSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKC 20 AA PAT REGION 1..20 note Synthetic: Artificial sequence source 1..20 mol_type protein organism synthetic construct SGGSGGGGSGGGSGGGGSLQ 209 AA PAT REGION 1..209 note Synthetic: IL-15R-alpha sushi domain + linker + Il-15 N65S source 1..209 mol_type protein organism synthetic construct CPPPMSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKCIRDPALVHQRPAPPSGGSGGGGSGGGSGGGGSLQNWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVESLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS 34 AA PAT REGION 1..34 note Synthetic: Artificial sequence source 1..34 mol_type protein organism synthetic construct IRDPALVHQRPAPPSGGSGGGGSGGGSGGGGSLQ 14 AA PAT REGION 1..14 note Synthetic: Artificial sequence source 1..14 mol_type protein organism synthetic construct IRDPALVHQRPAPP 5 AA PAT REGION 1..5 note Synthetic: 3F2 CDR1H source 1..5 mol_type protein organism synthetic construct AQYMY 17 AA PAT REGION 1..17 note Synthetic: 3F2 CDR2H source 1..17 mol_type protein organism synthetic construct RINPTSGNTVYAQKLQG 7 AA PAT REGION 1..7 note Synthetic: 3F2 CDR3H source 1..7 mol_type protein organism synthetic construct GINWFDP 7 AA PAT REGION 1..7 note Synthetic: 50A10 CDR1L source 1..7 mol_type protein organism synthetic construct SQDISNY 000 6 AA PAT REGION 1..6 note Synthetic: 50A10 CDR3L source 1..6 mol_type protein organism synthetic construct SYRSPF 8 AA PAT REGION 1..8 note Synthetic: 3F2 CDR1H source 1..8 mol_type protein organism synthetic construct GYTFTAQY 8 AA PAT REGION 1..8 note Synthetic: 3F2 CDR2H source 1..8 mol_type protein organism synthetic construct INPTSGNT 9 AA PAT REGION 1..9 note Synthetic: 3F2 CDR3H source 1..9 mol_type protein organism synthetic construct ARGINWFDP 7 AA PAT REGION 1..7 note Synthetic: 50A10 CDR1L source 1..7 mol_type protein organism synthetic construct SQDISNY 000 6 AA PAT REGION 1..6 note Synthetic: 50A10 CDR3L source 1..6 mol_type protein organism synthetic construct SYRSPF 7 AA PAT REGION 1..7 note Synthetic: 3F2 CDR1H source 1..7 mol_type protein organism synthetic construct GYTFTAQ 4 AA PAT REGION 1..4 note Synthetic: 3F2 CDR2H source 1..4 mol_type protein organism synthetic construct PTSG 5 AA PAT REGION 1..5 note Synthetic: 3F2 CDR3H source 1..5 mol_type protein organism synthetic construct INWFD 11 AA PAT REGION 1..11 note Synthetic: 50A10 CDR1L source 1..11 mol_type protein organism synthetic construct QASQDISNYIN 7 AA PAT REGION 1..7 note Synthetic: 50A10 CDR2L source 1..7 mol_type protein organism synthetic construct AASRLQS 9 AA PAT REGION 1..9 note Synthetic: 50A10 CDR3L source 1..9 mol_type protein organism synthetic construct QQSYRSPFT