0195-PA-004RU CN CN2021109086972 2021-08-09 Changchun Intellicrown Pharmaceutical Co., Ltd. NOVEL MUTANT OF RECOMBINANT GANODERMA LUCIDUM IMMUNOMODULATORY PROTEIN AND USE THEREOF 33 40 DNA PAT misc_feature 1..40 note Forward Primer F source 1..40 mol_type other DNA organism synthetic construct ccgctcgagaaaagaatgtctgatactgctttgatcttca 27 DNA PAT misc_feature 1..27 note Reverse Primer R source 1..27 mol_type other DNA organism synthetic construct gctctagactagttccattgagcgata 27 DNA PAT misc_feature 1..27 note Forward Primer F3 source 1..27 mol_type other DNA organism synthetic construct ccgctcgagaaaagagaggctgaagct 23 DNA PAT misc_feature 1..23 note Reverse Primer R3 source 1..23 mol_type other DNA organism synthetic construct gctctagatcactagttccattg 23 DNA PAT misc_feature 1..23 note Forward Primer M13F source 1..23 mol_type other DNA organism synthetic construct cccagtcacgacgttgtaaaacg 23 DNA PAT misc_feature 1..23 note Reverse Primer M13R source 1..23 mol_type other DNA organism synthetic construct agcggataacaatttcacacagg 70 DNA PAT misc_feature 1..70 note Forward Primer F2 source 1..70 mol_type other DNA organism synthetic construct ccgctcgagaaaagagaagaggccgaagccgaagcagaacctaaaatgtctgatactgctttgatcttca 4 AA PAT REGION 1..4 note spacer peptide sequence 1 source 1..4 mol_type protein organism synthetic construct EAEA 10 AA PAT REGION 1..10 note spacer peptide sequence 2 source 1..10 mol_type protein organism synthetic construct EEAEAEAEPK 111 AA PAT REGION 1..111 note rLZ-8 source 1..111 mol_type protein organism synthetic construct MSDTALIFRLAWDVKKLSFDYTPNWGRGNPNNFIDTVTFPKVLTDKAYTYRVAVSGRNLGVKPSYAVESDGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 115 AA PAT REGION 1..115 note EAEA-rLZ-8 source 1..115 mol_type protein organism synthetic construct EAEAMSDTALIFRLAWDVKKLSFDYTPNWGRGNPNNFIDTVTFPKVLTDKAYTYRVAVSGRNLGVKPSYAVESDGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 121 AA PAT REGION 1..121 note EEAEAEAEPK-rLZ-8 source 1..121 mol_type protein organism synthetic construct EEAEAEAEPKMSDTALIFRLAWDVKKLSFDYTPNWGRGNPNNFIDTVTFPKVLTDKAYTYRVAVSGRNLGVKPSYAVESDGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 111 AA PAT REGION 1..111 note rLZ-8 mutant (D70K) source 1..111 mol_type protein organism synthetic construct MSDTALIFRLAWDVKKLSFDYTPNWGRGNPNNFIDTVTFPKVLTDKAYTYRVAVSGRNLGVKPSYAVESKGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 115 AA PAT REGION 1..115 note EAEA-rLZ-8 mutant (D70K) source 1..115 mol_type protein organism synthetic construct EAEAMSDTALIFRLAWDVKKLSFDYTPNWGRGNPNNFIDTVTFPKVLTDKAYTYRVAVSGRNLGVKPSYAVESKGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 121 AA PAT REGION 1..121 note EEAEAEAEPK-rLZ-8 mutant (D70K) source 1..121 mol_type protein organism synthetic construct EEAEAEAEPKMSDTALIFRLAWDVKKLSFDYTPNWGRGNPNNFIDTVTFPKVLTDKAYTYRVAVSGRNLGVKPSYAVESKGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 111 AA PAT REGION 1..111 note rLZ-8 mutant 1 (R9A) source 1..111 mol_type protein organism synthetic construct MSDTALIFALAWDVKKLSFDYTPNWGRGNPNNFIDTVTFPKVLTDKAYTYRVAVSGRNLGVKPSYAVESDGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 111 AA PAT REGION 1..111 note rLZ-8 mutant 2 (L17K/D70K) source 1..111 mol_type protein organism synthetic construct MSDTALIFRLAWDVKKKSFDYTPNWGRGNPNNFIDTVTFPKVLTDKAYTYRVAVSGRNLGVKPSYAVESKGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 111 AA PAT REGION 1..111 note rLZ-8 mutant 3 (D20H) source 1..111 mol_type protein organism synthetic construct MSDTALIFRLAWDVKKLSFHYTPNWGRGNPNNFIDTVTFPKVLTDKAYTYRVAVSGRNLGVKPSYAVESDGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 111 AA PAT REGION 1..111 note rLZ-8 mutant 4 (L17K) source 1..111 mol_type protein organism synthetic construct MSDTALIFRLAWDVKKKSFDYTPNWGRGNPNNFIDTVTFPKVLTDKAYTYRVAVSGRNLGVKPSYAVESDGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 111 AA PAT REGION 1..111 note rLZ-8 mutant 5 (K46E/K74E) source 1..111 mol_type protein organism synthetic construct MSDTALIFRLAWDVKKLSFDYTPNWGRGNPNNFIDTVTFPKVLTDEAYTYRVAVSGRNLGVKPSYAVESDGSQEVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 111 AA PAT REGION 1..111 note rLZ-8 mutant 6 (K46E) source 1..111 mol_type protein organism synthetic construct MSDTALIFRLAWDVKKLSFDYTPNWGRGNPNNFIDTVTFPKVLTDEAYTYRVAVSGRNLGVKPSYAVESDGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 115 AA PAT REGION 1..115 note EAEA+mutant 1 (R9A) source 1..115 mol_type protein organism synthetic construct EAEAMSDTALIFALAWDVKKLSFDYTPNWGRGNPNNFIDTVTFPKVLTDKAYTYRVAVSGRNLGVKPSYAVESDGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 115 AA PAT REGION 1..115 note EAEA+mutant 2 (L17K/D70K) source 1..115 mol_type protein organism synthetic construct EAEAMSDTALIFRLAWDVKKKSFDYTPNWGRGNPNNFIDTVTFPKVLTDKAYTYRVAVSGRNLGVKPSYAVESKGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 115 AA PAT REGION 1..115 note EAEA+mutant 3 (D20H) source 1..115 mol_type protein organism synthetic construct EAEAMSDTALIFRLAWDVKKLSFHYTPNWGRGNPNNFIDTVTFPKVLTDKAYTYRVAVSGRNLGVKPSYAVESDGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 115 AA PAT REGION 1..115 note EAEA+mutant 4 (L17K) source 1..115 mol_type protein organism synthetic construct EAEAMSDTALIFRLAWDVKKKSFDYTPNWGRGNPNNFIDTVTFPKVLTDKAYTYRVAVSGRNLGVKPSYAVESDGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 115 AA PAT REGION 1..115 note EAEA+mutant 5 (K46E/K74E) source 1..115 mol_type protein organism synthetic construct EAEAMSDTALIFRLAWDVKKLSFDYTPNWGRGNPNNFIDTVTFPKVLTDEAYTYRVAVSGRNLGVKPSYAVESDGSQEVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 115 AA PAT REGION 1..115 note EAEA+mutant 6 (K46E) source 1..115 mol_type protein organism synthetic construct EAEAMSDTALIFRLAWDVKKLSFDYTPNWGRGNPNNFIDTVTFPKVLTDEAYTYRVAVSGRNLGVKPSYAVESDGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 121 AA PAT REGION 1..121 note EEAEAEAEPK+mutant 1 (R9A) source 1..121 mol_type protein organism synthetic construct EEAEAEAEPKMSDTALIFALAWDVKKLSFDYTPNWGRGNPNNFIDTVTFPKVLTDKAYTYRVAVSGRNLGVKPSYAVESDGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 121 AA PAT REGION 1..121 note EEAEAEAEPK+mutant 2 (L17K/D70K) source 1..121 mol_type protein organism synthetic construct EEAEAEAEPKMSDTALIFRLAWDVKKKSFDYTPNWGRGNPNNFIDTVTFPKVLTDKAYTYRVAVSGRNLGVKPSYAVESKGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 121 AA PAT REGION 1..121 note EEAEAEAEPK+mutant 3 (D20H) source 1..121 mol_type protein organism synthetic construct EEAEAEAEPKMSDTALIFRLAWDVKKLSFHYTPNWGRGNPNNFIDTVTFPKVLTDKAYTYRVAVSGRNLGVKPSYAVESDGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 121 AA PAT REGION 1..121 note EEAEAEAEPK+mutant 4 (L17K) source 1..121 mol_type protein organism synthetic construct EEAEAEAEPKMSDTALIFRLAWDVKKKSFDYTPNWGRGNPNNFIDTVTFPKVLTDKAYTYRVAVSGRNLGVKPSYAVESDGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 121 AA PAT REGION 1..121 note EEAEAEAEPK+mutant 5 (K46E/K74E) source 1..121 mol_type protein organism synthetic construct EEAEAEAEPKMSDTALIFRLAWDVKKLSFDYTPNWGRGNPNNFIDTVTFPKVLTDEAYTYRVAVSGRNLGVKPSYAVESDGSQEVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN 121 AA PAT REGION 1..121 note EEAEAEAEPK+mutant 6 (K46E) source 1..121 mol_type protein organism synthetic construct EEAEAEAEPKMSDTALIFRLAWDVKKLSFDYTPNWGRGNPNNFIDTVTFPKVLTDEAYTYRVAVSGRNLGVKPSYAVESDGSQKVNFLEYNSGYGIADTNTIQVFVVDPDTNNDFIIAQWN