US X22492 US 63/260496 2021-08-23 Eli Lilly and Company COMPOUNDS AND METHODS FOR LIQUID PHASE SYNTHESIS 32 19 AA PAT source 1..19 mol_type protein organism synthetic construct MOD_RES 4 note Glutamic acid at position 4 has a protected side chain (-CO2tBu) MOD_RES 5 note Tyrosine at position 5 has a protected side chain (-OtBu) MOD_RES 8 note Glutamic acid at position 8 has a protected side chain (-CO2tBu) MOD_RES 12 note Serine at position 12 has a protected side chain (-OtBu) MOD_RES 13 note Serine at position 13 has a protected side chain (OtBu) MOD_RES 19 note Serine at position 19 has a protected side chain (-OtBu) MOD_RES 19 note Serine at position 19 is amidated with a hydrophilic linker (Rink-(AEEA)6-NH2) AFIEYLLEGGPSSGAPPPS 19 AA PAT source 1..19 mol_type protein organism synthetic construct MOD_RES 19 note Serine at position 19 is amidated as a C-terminal primary amide AFIEYLLEGGPSSGAPPPS 10 AA PAT source 1..10 mol_type protein organism synthetic construct MOD_RES 3 note Serine at position 3 has a protected side chain (-OtBu) MOD_RES 4 note Serine at position 4 has a protected side chain (-OtBu) MOD_RES 10 note Serine at position 10 has a protected side chain (-OtBu) MOD_RES 10 note Serine at position 10 is amidated with a hydrophilic linker (Ramage-(AEEA)10-NH2) GPSSGAPPPS 10 AA PAT source 1..10 mol_type protein organism synthetic construct MOD_RES 3 note Serine at position 3 has a protected side chain (-OtBu) MOD_RES 4 note Serine at position 4 has a protected side chain (-OtBu) MOD_RES 10 note Serine at position 10 is amidated as a C-terminal primary amide GPSSGAPPPS 10 AA PAT source 1..10 mol_type protein organism synthetic construct MOD_RES 3 note Serine at position 3 has a protected side chain (-OtBu) MOD_RES 4 note Serine at position 4 has a protected side chain (-OtBu) MOD_RES 10 note Serine at position 10 has a protected side chain (-OtBu) MOD_RES 10 note Serine at position 10 is amidated with a hydrophilic linker (Rink-(AEEA)2-NH2) GPSSGAPPPS 8 AA PAT source 1..8 mol_type protein organism synthetic construct MOD_RES 1 note Phenylalanine at position 1 is -Fmoc protected MOD_RES 3 note Glutamine residue at position 3 has a protected side chain (-CONH-trityl) MOD_RES 4 note Tryptophan at position 4 has a protected side chain (-NBoc-) MOD_RES 8 note Glycine at position 8 is esterified with a hydrophilic linker (HMPA-(AEEA)10-NH2) FVQWLIAG 8 AA PAT source 1..8 mol_type protein organism synthetic construct MOD_RES 1 note Phenylalanine at position 1 is -Fmoc protected MOD_RES 3 note Glutamine at position 3 has a protected side chain (-CONH-trityl) MOD_RES 4 note Tryptophan at position 4 has a protected side chain (-NBoc-) FVQWLIAG 8 AA PAT source 1..8 mol_type protein organism synthetic construct MOD_RES 1 note Phenylalanine at position 1 is -Fmoc protected MOD_RES 3 note Glutamine at position 3 has a protected side chain (-CONH-trityl) MOD_RES 4 note Tryptophan at position 4 has a protected side chain (-NBoc-) MOD_RES 8 note Glycine at position 8 is esterified with a hydrophilic linker (HMPB-(AEEA)10-NH2) FVQWLIAG 7 AA PAT source 1..7 mol_type protein organism synthetic construct MOD_RES 1 note Aspartic acid at position 1 is -Fmoc protected MOD_RES 1 note Aspartic acid at position 1 has a protected side chain (-CO2tBu) MOD_RES 2 note Lysine at position 2 has a protected side chain (-NH-Boc) MOD_RES 5 note Glutamine at position 5 has a protected side chain (-CONH-trityl) MOD_RES 6 note Lysine at position 6 is chemically modified by conjugation of the epsilon-amino group of the Lys side chain with -(AEEA)2-gamma glutamic acid(OtBu)-CO-(CH2)18-CO2tBu MOD_RES 7 note Alanine at position 7 is esterified with a hydrophilic linker (HMPB-(AEEA)10-NH2) DKIAQKA 7 AA PAT source 1..7 mol_type protein organism synthetic construct MOD_RES 1 note Aspartic acid at position 1 is -Fmoc protected MOD_RES 2 note Lysine at position 2 has a protected side chan (-NH-Boc) MOD_RES 1 note Aspartic acid at position 1 has a protected side chain (-CO2tBu) MOD_RES 5 note Glutamine at position 5 has a protected side chain (-CONH-trityl) MOD_RES 6 note Lysine at position 6 is chemically modified through conjugation to the epsilon-amino group of the Lys side-chain with (AEEA)2-gamma glutamic acid(OtBu)-CO-(CH2)18-CO2tBu DKIAQKA 14 AA PAT source 1..14 mol_type protein organism synthetic construct MOD_RES 1 note Tyrosine at position 1 is -Boc protected MOD_RES 1 note Tyrosine at position 1 has a protected side chain (-OtBu) NON_STD 2 note X at position 2 is nonnaturally occurring amino acid 2-Aminoisobutyric Acid (Aib) MOD_RES 3 note Glutamic acid at position 3 has a protected side chain (-CO2tBu) MOD_RES 5 note Threonine at position 5 has a protected side chain (-OtBu) MOD_RES 7 note Threonine at position 7 has a protected side chain (-OtBu) MOD_RES 8 note Serine at position 8 has a protected side chain (-OtBu) MOD_RES 9 note Aspartic acid at position 9 has a protected side chain (-CO2tBu) MOD_RES 10 note Tyrosine at position 10 has a protected side chain (-OtBu) MOD_RES 11 note Serine at position 11 has a protected side chain (-OtBu) NON_STD 13 note X at position 13 is nonnaturally occurring amino acid 2-Aminoisobutyric Acid (Aib) MOD_RES 14 note Leucine at position 14 is esterified with a hydrophilic linker (HMPB-(AEEA)10-NH2) YXEGTFTSDYSIXL 4 AA PAT source 1..4 mol_type protein organism synthetic construct MOD_RES 1 note Tyrosine at position 1 is -Boc protected MOD_RES 1 note Tyrosine at position 1 has a protected side chain (-OtBu) MOD_RES 2 note Alanine at position 2 is alpha-methylated (Aib) MOD_RES 3 note Glutamic acid at position 3 has a protected side chain (-CO2tBu) MOD_RES 4 note Glycine at position 4 is esterified with a hydrophilic linker (HMPB-(AEEA)10-NH2) YAEG 4 AA PAT source 1..4 mol_type protein organism synthetic construct MOD_RES 1 note Tyrosine at position 1 is -Boc protected MOD_RES 1 note Tyrosine at position 1 has a protected side chain (-OtBu) MOD_RES 2 note Alanine at position 2 is alpha-methylated (Aib) MOD_RES 3 note Gutamic acid at position 3 has a protected side chain (-CO2tBu) YAEG 4 AA PAT source 1..4 mol_type protein organism synthetic construct MOD_RES 1 note Tyrosine at position 1 is -Boc protected MOD_RES 1 note Tyrosine at position 1 has a protected side chain (-OtBu) MOD_RES 2 note Alanine at position 2 is alpha-methylated (Aib) MOD_RES 3 note Glutamic acid at position 3 has a protected side chain (-CO2tBu) MOD_RES 4 note Glycine at position 4 is esterified with a hydrophilic linker (HMPB-(AEEA)4-NH2) YAEG 4 AA PAT source 1..4 mol_type protein organism synthetic construct MOD_RES 1 note Tyrosine at position 1 is -Boc protected MOD_RES 1 note Tyrosine at position 1 has a protected side chain (-OtBu) MOD_RES 2 note Alanine at position 2 is alpha-methylated (Aib) MOD_RES 3 note Glutamic acid at position 3 has a protected side chain (-CO2tBu) MOD_RES 4 note Glycine at position 4 is esterified with a hydrophilic linker (HMPB-(AEEA)2-NH2) YAEG 4 AA PAT source 1..4 mol_type protein organism synthetic construct MOD_RES 1 note Tyrosine at position 1 is -Boc protected MOD_RES 1 note Tyrosine at position 1 has a protected side chain (-OtBu) MOD_RES 2 note Alanine at position 2 is alpha-methylated (Aib) MOD_RES 3 note Glutamic acid at position 3 has a protected side chain (-CO2tBu) MOD_RES 4 note Glycine at position 4 is esterified with a hydrophilic linker (HMPB-(AEEA)6-NH2) YAEG 4 AA PAT source 1..4 mol_type protein organism unidentified MOD_RES 1 note Tyrosine at position 1 is -Boc protected MOD_RES 1 note Tyrosine at position 1 has a protected side chain (-OtBu) MOD_RES 2 note Alanine at position 2 is alpha-methylated (Aib) MOD_RES 3 note Glutamic acid at position 3 has a protected side chain (-CO2tBu) MOD_RES 4 note Glycine at position 4 is esterified with a hydrophilic linker (HMPA-(AEEA)10-NH2) YAEG 4 AA PAT source 1..4 mol_type protein organism synthetic construct MOD_RES 1 note Tyrosine at position 1 is -Boc protected MOD_RES 1 note Tyrosine at position 1 has a protected side chain (-OtBu) MOD_RES 2 note Alanine at position 2 is alpha-methylated (Aib) MOD_RES 3 note Glutamic acid at position 3 has a protected side chain (-CO2tBu) MOD_RES 4 note Glycine at position 4 is esterified with a hydrophilic side chain (HMPA-(AEEA)2-NH2) YAEG 10 AA PAT source 1..10 mol_type protein organism synthetic construct MOD_RES 3 note Serine at position 3 has a protected side chain (-OtBu) MOD_RES 4 note Serine at position 4 has a protected side chain (-OtBu) MOD_RES 10 note Serine at position 10 has a protected side chain (-OtBu) MOD_RES 10 note Serine at position 10 is amidated with a hydrophilic linker (Rink-(AEEA)6-NH2) GPSSGAPPPS 18 AA PAT source 1..18 mol_type protein organism unidentified MOD_RES 1 note Phenylalanine at position 1 is -Fmoc protected MOD_RES 3 note Glutamine at position 3 has a protected side chain (-CONH-trityl) MOD_RES 4 note Tryptophan at position 4 has a protected side chain (-NBoc-) MOD_RES 11 note Serine at position 11 has a protected side chain (-OtBu) MOD_RES 12 note Serine at position 12 has a protected side chain (-OtBu) MOD_RES 18 note Serine at position 18 has a protected side chain (-OtBu) MOD_RES 18 note Serine at position 18 is amidated with a hydrophilic linker (Rink-(AEEA)6-NH2) FVQWLIAGGPSSGAPPPS 18 AA PAT source 1..18 mol_type protein organism synthetic construct MOD_RES 3 note Glutamine at position 3 has a protected side chain (-CONH-trityl) MOD_RES 4 note Tryptophan at position 4 has a protected side chain (-NBoc-) MOD_RES 11 note Serine at position 11 has a protected side chain (-OtBu) MOD_RES 12 note Serine at position 12 has a protected side chain (-OtBu) MOD_RES 18 note Serine at position 18 has a protected side chain (-OtBu) MOD_RES 18 note Serine at position 18 is amidated with a hydrophilic linker (Rink-(AEEA)6-NH2) FVQWLIAGGPSSGAPPPS 25 AA PAT source 1..25 mol_type protein organism synthetic construct MOD_RES 1 note Aspartic acid at position 1 has a protected side chain (-CO2tBu) MOD_RES 2 note Lysine at position 2 has a protected side chain (-NH-Boc) MOD_RES 5 note Glutamine at position 5 has a protected side chain (-CONH-trityl) MOD_RES 6 note Lysine at position 6 is chemically modified by conjugation of the epsilon-amino group of the Lys side chain with (AEEA)2-gamma glutamic acid(OtBu)-CO-(CH2)18-CO2tBu MOD_RES 10 note Glutamine at position 10 has a protected side chain (-CONH-trityl) MOD_RES 11 note Tryptophan at position 11 has a protected side chain (-NBoc-) MOD_RES 18 note Serine at position 18 has a protected side chain (-OtBu) MOD_RES 19 note Serine at position 19 has a protected side chain (-OtBu) MOD_RES 25 note Serine at position 25 has a protected side chain (-OtBu) MOD_RES 25 note Serine at position 25 is amidated with a hydrophilic linker (Rink-(AEEA)6-NH2) DKIAQKAFVQWLIAGGPSSGAPPPS 32 AA PAT source 1..32 mol_type protein organism synthetic construct MOD_RES 1 note Serine at position 1 has a protected side chain (-OtBu) MOD_RES 2 note Aspartic acid at position 2 has a protected side chain (-CO2tBu) MOD_RES 3 note Tyrosine at position 3 has a protected side chain (-OtBu) MOD_RES 4 note Serine at position 4 has a protected side chain (-OtBu) NON_STD 6 note X at position 6 is nonnaturally occurring amino acid 2-Aminoisobutyric Acid (Aib) MOD_RES 8 note Aspartic acid at position 8 has a protected side chain (-CO2tBu) MOD_RES 9 note Lysine at position 9 has a protected side (-NHBoc) MOD_RES 12 note Glutamine at position 12 has a protected side chain (-CONH-trityl) MOD_RES 13 note Lysine at position 13 is chemically modified through conjugation to the epsilon-amino group of the Lys side-chain with -(AEEA)2-gamma glutamic acid(OtBu)-CO-(CH2)18-CO2tBu MOD_RES 17 note Glutamine at position 17 has a protected side chain (-CONH-trityl) MOD_RES 18 note Tryptophan at position 18 has a protected side chain (-NBoc-) MOD_RES 25 note Serine at position 25 has a protected side chain (-OtBu) MOD_RES 26 note Serine at position 26 has a protected side chain (-OtBu) MOD_RES 32 note Serine at position 32 has a protected side chain (-OtBu) MOD_RES 32 note Serine at position 32 is amidated with a hydrophilic linker (Rink-(AEEA)10-NH2) SDYSIXLDKIAQKAFVQWLIAGGPSSGAPPPS 4 AA PAT source 1..4 mol_type protein organism synthetic construct MOD_RES 1 note Proline at position 1 is -Fmoc protected MOD_RES 4 note Serine at position 4 has a protected side chain (-OtBu) PPPS 4 AA PAT source 1..4 mol_type protein organism synthetic construct MOD_RES 1 note Serine at position 1 is -Fmoc protected MOD_RES 1 note Serine at position 1 has a protected side chain (-OtBu) MOD_RES 2 note Serine at position 2 has a protected side chain (-OtBu) SSGA 4 AA PAT source 1..4 mol_type protein organism synthetic construct MOD_RES 1 note Alanine at position 1 is -Fmoc protected AAGP 4 AA PAT source 1..4 mol_type protein organism synthetic construct MOD_RES 1 note Glutamine at position 1 is -Fmoc protected MOD_RES 1 note Glutamine at position 1 has a protected side chain (-CONH-trityl) MOD_RES 2 note Tryptophan at position 2 has a protected side chain (-NBoc-) QWLI 4 AA PAT source 1..4 mol_type protein organism synthetic construct MOD_RES 1 note Lysine at position 1 is -Fmoc protected MOD_RES 1 note Lysine at position 1 is chemically modified by conjugation of the epsilon-amino group of the Lys side chain with (AEEA)2-gamma glutamic acid(OtBu)-CO-(CH2)18-CO2tBu KAFV 4 AA PAT source 1..4 mol_type protein organism synthetic construct MOD_RES 1 note Lysine at position 1 is -Fmoc protected MOD_RES 4 note Glutamine at position 4 has a protected side chain (-CONH-trityl) KIAQ 4 AA PAT source 1..4 mol_type protein organism synthetic construct MOD_RES 1 note Isoleucine at position 1 is -Fmoc protected MOD_RES 2 note Alanine at position 2 is alpha-methylated (Aib) MOD_RES 4 note Aspartic acid at position 4 has a protected side chain (-CO2tBu) IALD 4 AA PAT source 1..4 mol_type protein organism synthetic construct MOD_RES 1 note Serine at position 1 is -Fmoc protected MOD_RES 1 note Serine at position 1 has a protected side chain (-OtBu) MOD_RES 2 note Aspartic acid at position 2 has a protected side chain (-CO2tBu) MOD_RES 3 note Tyrosine at position 3 has a protected side chain (-OtBu) MOD_RES 4 note Serine at position 4 has a protected side chain (-OtBu) SDYS 39 AA PAT source 1..39 mol_type protein organism synthetic construct NON_STD 2 note X at position 2 is nonnaturally occurring amino acid 2-Aminoisobutyric Acid (Aib) NON_STD 13 note X at position 13 is nonnaturally occurring amino acid 2-Aminoisobutyric Acid (Aib) MOD_RES 20 note Lysine at position 20 is is chemically modified through conjugation to the epsilon-amino group of the Lys side-chain with (AEEA)2-gamma glutamic acid-CO-(CH2)18-CO2H MOD_RES 39 note Serine at position 39 is amidated as a C-terminal primary amide YXEGTFTSDYSIXLDKIAQKAFVQWLIAGGPSSGAPPPS