US PCT/US2022/074075 2022-07-22 0019240.01250WO1 US 63/225,193 2021-07-23 THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK CHARACTERIZATION OF POTENT AND BROADLY NEUTRALIZING MONOCLONAL ANTIBODIES AGAINST SARS-COV-2, ITS VARIANTS, AND RELATED CORONAVIRUSES AND METHODS OF USE 141 123 AA PAT source 1..123 mol_type protein organism synthetic construct QMQLVQSGPEVKKPGTSVKVSCKASGFTFISSAMQWVRQARGQRLEWIGWIVVGSGNTNYAQKFQERVSITRDMSTSTAYMELSSLRSEDTAVYYCAAPYCNSTNCYDAFNIWGQGTMVTVSS 108 AA PAT source 1..108 mol_type protein organism synthetic construct EIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGRSPWTFGQGTKVDIK 130 AA PAT source 1..130 mol_type protein organism synthetic construct QLQLQESGPGLVKPSETLSLTCTVSGGSISSSNFYWGWIRQPPGKGLEWIASITYSGRTFYNPSLKSRVAISVDTSKNQFSLKLSSVTAADTAVYYCARTFPSYYDRSGYHYLNYGMDVWGQGTTVTVSS 110 AA PAT source 1..110 mol_type protein organism synthetic construct NFMLTQPHSMSESPGKTVTISCTRSSGSIASNYVQWYQQRPGSSPTTVIYEDNQRPSGVPDRFSGSIDSSSNSASLTISGLKTEDEADYYCQSYDSSSWVFGGGTKLTVL 129 AA PAT source 1..129 mol_type protein organism synthetic construct QVQLQESGPGLVKPSQTLSLTCTVSGGSISSGGYYWSWIRQHPGKGLEWIGYIYYSGSTYYNPSLKSRVTISVDTSKNQFSLKVSSVTAADTAVYYCASSNYYDGSGYNLLESYGMDVWGQGTSVTVSS 112 AA PAT source 1..112 mol_type protein organism synthetic construct QSVLTQPPSVSGAPGQRVTISCTGSSSNIGAGYDVHWYQQLPGTAPKLLIYGNSNRPSGVPDRFSGSRSGTSASLAITGLQAEDEADYYCQSYDSSLSGSWVFGGGTKVTVL 124 AA PAT source 1..124 mol_type protein organism synthetic construct QVQLVQSGAEVKKPGSSVKVSCKASGGTFSNYAISWVRQAPGQGLEWMGGFIRIFDTAIYAQKFQGRVTITADESTSTAYMDLSSLRSEDTAVYYCARSPYYYDKSGYYYFDHWGQGTLVTVSS 107 AA PAT source 1..107 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQNISSYLNWYQQKPGKAPNLLIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSPQWTFGQGTKVEIK 123 AA PAT source 1..123 mol_type protein organism synthetic construct EVQLVQSGAEVKKPGESLKMSCKGSGYNFTFYWIGWVRQMPGKGLEWMGSIYPGDSETRYSPSFQGQVTISAATSISTAYLQWSSLKASDAAMYYCTARLSGVQTALEYFDFWGQGTLVTVSS 107 AA PAT source 1..107 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCQASQDITNHLNWYQLRPGKAPKLLIYDASNLDAGVPSRFSGSGSGTDFTFTISSLQPEDIATYYCQQYDNLLLTFGGGTKVEIK 126 AA PAT source 1..126 mol_type protein organism synthetic construct QVQLVESGGGVVQPGRSLRLSCAASGFIFSDFGMHWVRQAPGKGLEWVAVISYDGSNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCKARDYYDISGFYHPPNFDYWGQGTLVTVSS 108 AA PAT source 1..108 mol_type protein organism synthetic construct SYELTQPPSVSVSPGQTARIPCSGHTLPKQYAYWYQQKPGQAPVLVIYKDSERPSGIPERFSGSSSGTTVTLTISGVQAEDEANYYCQSADSSGTYWVFGGGTKLTVL 120 AA PAT source 1..120 mol_type protein organism synthetic construct QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYDMHWVRQAPGKGLEWVAVISYDGSSKFYAESVKGRFTISRDNSKNTLYLQMNSLRAEETAVYYCVKDGEQLVPLFDYWGQGTLVTVSS 108 AA PAT source 1..108 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPGVTFGPGTKVDIK 132 AA PAT source 1..132 mol_type protein organism synthetic construct QVQLVESGGGVVQPGRSLRLSCAASGFTFSNYGMHWVRQAPGKGLEWVAIISFDGSNRYYADSVKGRFTISRDNSKNTLYLQMDSLRAEDTAVYYCAKDLSFYYDISTGYYPQSYNSGTDVWGQGTTVTVSS 108 AA PAT source 1..108 mol_type protein organism synthetic construct SYELTQPPSVSVSPGQTASITCSGDKLGDKYACWYQQKPGQSPVLVIYQDNKRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCQGWDRSTGYYVFGTGTKVTVL 133 AA PAT source 1..133 mol_type protein organism synthetic construct QVQLVESGGGVVQPGRSLRLSCEASGFTFNTYDMHWARQAPGKGLEWVAVIWYDGSNKFYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARDRTPVYDILTGYYWPPPRVDGMDVWGQGTTVTVSS 112 AA PAT source 1..112 mol_type protein organism synthetic construct DNVMTQTPFSLSVTPGQPASISCKSSQSLLHSDGKTYLYWYLQKPGQSPQLLIYEVSNRFSGVPERFSGSGSGTDFTLKISRVEAEDVGVYYCMQSIQLPFTFGQGTKLEIK 130 AA PAT source 1..130 mol_type protein organism synthetic construct QLQLQESGPGLVKPSETLSLTCTVSGGSISSSNFYWGWIRQPPGKGLEWIASITYSGRTFYNPSLKSRVAISVDTSKNQFSLKLSSVTAADTAVYYCARTFPSYYDRSGYHYLNYGMDVWGQGTTVTVSS 128 AA PAT source 1..128 mol_type protein organism synthetic construct QVQLQESGPGLVKPSETLSLTCTVSGGSVSSSNYYWSWIRQPPGKGLEWIGYMYYSGSTKYNPSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCAREVYYYDRSGYYASDGFDIWGQGTMVTVSS 129 AA PAT source 1..129 mol_type protein organism synthetic construct QVQLQESGPGLVKPSETLSVTCTVSGGSISSSRYYWGWIRQPPGKGLEWIGSIYYSGSTYYNPSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCARHAAAYYDRSGYYFIEYFQHWGQGTLVTVSS 129 AA PAT source 1..129 mol_type protein organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYYMHWVRQAPGQGLEWMGIINSSGGSTSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARPPRNYYDRSGYYQRAEYFQHWGQGTLVTVSS 9 AA PAT source 1..9 mol_type protein organism synthetic construct YYYDSSGYY 15 AA PAT source 1..15 mol_type protein organism synthetic construct CVKDGEQLVPLFDYW 24 AA PAT source 1..24 mol_type protein organism synthetic construct CARTFPSYYDRSGYHYLNYGMDVW 23 AA PAT source 1..23 mol_type protein organism synthetic construct CASSNYYDGSGYNLLESYGMDVW 12 AA PAT source 1..12 mol_type protein organism synthetic construct CQQSYSTPGVTF 11 AA PAT source 1..11 mol_type protein organism synthetic construct CQSYDSSSWVF 14 AA PAT source 1..14 mol_type protein organism synthetic construct CQSYDSSLSGSWVF 34 AA PAT source 1..34 mol_type protein organism synthetic construct QLQLQESGPGLVKPSETLSLTCTVSGGSISSSSY 61 AA PAT source 1..61 mol_type protein organism synthetic construct WGWIRQPPGKGLEWIGSIYYSGSTYYNPSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYY 25 AA PAT source 1..25 mol_type protein organism synthetic construct QLQLQESGPGLVKPSETLSLTCTVS 61 AA PAT source 1..61 mol_type protein organism synthetic construct WGWIRQPPGKGLEWIASITYSGRTFYNPSLKSRVAISVDTSKNQFSLKLSSVTAADTAVYY 21 AA PAT source 1..21 mol_type protein organism synthetic construct ARTFPSYYDRSGYHYLNYGMD 11 AA PAT source 1..11 mol_type protein organism synthetic construct WGQGTTVTVSS 90 AA PAT source 1..90 mol_type protein organism synthetic construct NFMLTQPHSVSESPGKTVTISCTRSSGSIASNYVQWYQQRPGSSPTTVIYEDNQRPSGVPDRFSGSIDSSSNSASLTISGLKTEDEADYY 32 AA PAT source 1..32 mol_type protein organism synthetic construct NFMLTQPHSMSESPGKTVTISCTRSSGSIASN 57 AA PAT source 1..57 mol_type protein organism synthetic construct VQWYQQRPGSSPTTVIYEDNQRPSGVPDRFSGSIDSSSNSASLTISGLKTEDEADYY 19 AA PAT source 1..19 mol_type protein organism synthetic construct QSYDSSSWVFGGGTKLTVL 10 AA PAT source 1..10 mol_type protein organism synthetic construct VARIANT 4 note I or V VARIANT 8 note N or R VARIANT 9 note F or Y GGSXSSSXXY 8 AA PAT source 1..8 mol_type protein organism synthetic construct GYTFTSYY 23 AA PAT source 1..23 mol_type protein organism synthetic construct VARIANT 3 note Any amino acid VARIANT 4 note Any amino acid VARIANT 5 note Any amino acid VARIANT 6 note Any amino acid VARIANT 7 note Any amino acid VARIANT 3..7 note This region may encompass 4-5 residues VARIANT 15 note Any amino acid VARIANT 16 note Any amino acid VARIANT 17 note Any amino acid VARIANT 18 note Any amino acid VARIANT 19 note Any amino acid VARIANT 20 note Any amino acid VARIANT 21 note Any amino acid VARIANT 22 note Any amino acid VARIANT 23 note Any amino acid VARIANT 15..23 note This region may encompass 8-9 residues ARXXXXXYYDRSGYXXXXXXXXX 5 AA PAT source 1..5 mol_type protein organism synthetic construct LYNSS 5 AA PAT source 1..5 mol_type protein organism synthetic construct LYNST 5 AA PAT source 1..5 mol_type protein organism synthetic construct LYNSA 5 AA PAT source 1..5 mol_type protein organism synthetic construct FYNST 9 AA PAT source 1..9 mol_type protein organism synthetic construct FKCYGVSPT 9 AA PAT source 1..9 mol_type protein organism synthetic construct FQCYGVSPT 9 AA PAT source 1..9 mol_type protein organism synthetic construct FKCYGVSAT 9 AA PAT source 1..9 mol_type protein organism synthetic construct FKCYGVSAI 9 AA PAT source 1..9 mol_type protein organism synthetic construct FKCYGVSPS 9 AA PAT source 1..9 mol_type protein organism synthetic construct FNCYGVSPS 6 AA PAT source 1..6 mol_type protein organism synthetic construct APAQTG 6 AA PAT source 1..6 mol_type protein organism synthetic construct APGQTG 6 AA PAT source 1..6 mol_type protein organism synthetic construct APGETG 4 AA PAT source 1..4 mol_type protein organism synthetic construct DDFT 4 AA PAT source 1..4 mol_type protein organism synthetic construct DDFL 4 AA PAT source 1..4 mol_type protein organism synthetic construct DDFM 4 AA PAT source 1..4 mol_type protein organism synthetic construct DEFT 5 AA PAT source 1..5 mol_type protein organism synthetic construct TSGNY 5 AA PAT source 1..5 mol_type protein organism synthetic construct QTGNY 5 AA PAT source 1..5 mol_type protein organism synthetic construct SSGNF 5 AA PAT source 1..5 mol_type protein organism synthetic construct STGNY 5 AA PAT source 1..5 mol_type protein organism synthetic construct TGGNY 5 AA PAT source 1..5 mol_type protein organism synthetic construct EGGNF 5 AA PAT source 1..5 mol_type protein organism synthetic construct VGGNY 14 AA PAT source 1..14 mol_type protein organism synthetic construct YNSAGGTCSSISQL 13 AA PAT source 1..13 mol_type protein organism synthetic construct FNPSGGTCSAEGL 13 AA PAT source 1..13 mol_type protein organism synthetic construct YSPGGQSCSAVGP 13 AA PAT source 1..13 mol_type protein organism synthetic construct YSPGGQSCSAIGP 13 AA PAT source 1..13 mol_type protein organism synthetic construct FSPDGKPCTPPAF 13 AA PAT source 1..13 mol_type protein organism synthetic construct FSPDGKPCTPPAL 14 AA PAT source 1..14 mol_type protein organism synthetic construct YQAGSTPCNGQVGL 14 AA PAT source 1..14 mol_type protein organism synthetic construct YQAGSKPCNGQTGL 14 AA PAT source 1..14 mol_type protein organism synthetic construct YQAGSTPCNGVEGF 188 AA PAT source 1..188 mol_type protein organism synthetic construct RFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSF 188 AA PAT source 1..188 mol_type protein organism synthetic construct RFPNITNLCPFGEVFNATTFASVYAWNRKRISNCVADYSVLYNSTSFSTFKCYGVSPTKLNDLCFTNVYADSFVVRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFHPTNGVGYQPYRVVVLSF 188 AA PAT source 1..188 mol_type protein organism synthetic construct RFPNITNLCPFGEVFNATTFASVYAWNRKRISNCVADYSVLYNSTSFSTFKCYGVSPTKLNDLCFTNVYADSFVVRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLKSYGFHPTNGVGYQPYRVVVLSF 169 AA PAT source 1..169 mol_type protein organism synthetic construct RFPNITNFCPFDKVFNATRFPNVYAWQRTKISDCIADYTVLYNSTSFSTFKCYGVSPSKLIDLCFTSVYADTFLIRFSEVRQIAPGETGVIADYNYKLPDDFTGCVLAWNTAQQDIGSYFYRSHRAVKLKPFERDLSSDENGVRTLSTYDFNPNVPLDYQATRVVVLSF 25 AA PAT source 1..25 mol_type protein organism synthetic construct AKDLSFYYDISTGYYPQSYNSGTDV 11 AA PAT source 1..11 mol_type protein organism synthetic construct QGWDRSTGYYV 26 AA PAT source 1..26 mol_type protein organism synthetic construct ARDRTPVYDILTGYYWPPPRVDGMDV 9 AA PAT source 1..9 mol_type protein organism synthetic construct MQSIQLPFT 96 AA PAT source 1..96 mol_type protein organism synthetic construct QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYGMHWVRQAPGKGLEWVAVISYDGSNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYC 87 AA PAT source 1..87 mol_type protein organism synthetic construct SYELTQPPSVSVSPGQTASITCSGDKLGDKYACWYQQKPGQSPVLVIYQDSKRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYC 96 AA PAT source 1..96 mol_type protein organism synthetic construct QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYGMHWVRQAPGKGLEWVAVIWYDGSNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYC 93 AA PAT source 1..93 mol_type protein organism synthetic construct DIVMTQTPLSLSVTPGQPASISCKSSQSLLHSDGKTYLYWYLQKPGQSPQLLIYEVSSRFSGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYC 11 DNA PAT source 1..11 mol_type other DNA organism synthetic construct tgtgcgaaaga 31 DNA PAT source 1..31 mol_type other DNA organism synthetic construct gtattacgatattttgactggttattataac 32 DNA PAT source 1..32 mol_type other DNA organism synthetic construct attactactactactacggtatggacgtctgg 10 AA PAT source 1..10 mol_type protein organism synthetic construct YYDILTGYYN 10 AA PAT source 1..10 mol_type protein organism synthetic construct YYYYYGMDVW 51 DNA PAT source 1..51 mol_type other DNA organism synthetic construct tgtgcgaaagatctatctttctactacgatatttcgactggatattatccc 29 DNA PAT source 1..29 mol_type other DNA organism synthetic construct agtcctacaactccggtacggacgtctgg 17 AA PAT source 1..17 mol_type protein organism synthetic construct CAKDLSFYYDISTGYYP 10 AA PAT source 1..10 mol_type protein organism synthetic construct QSYNSGTDVW 11 DNA PAT source 1..11 mol_type other DNA organism synthetic construct tgtgcgagaga 84 DNA PAT source 1..84 mol_type other DNA organism synthetic construct tgtgcgagagatagaacgcccgtttacgatattttgactggttattattggccccctccgagggtcgacggtatggacgtctgg 28 AA PAT source 1..28 mol_type protein organism synthetic construct CARDRTPVYDILTGYYWPPPRVDGMDVW 4 AA PAT source 1..4 mol_type protein organism synthetic construct RTPV 8 AA PAT source 1..8 mol_type protein organism synthetic construct GFTFISSA 8 AA PAT source 1..8 mol_type protein organism synthetic construct GFTFSSYD 10 AA PAT source 1..10 mol_type protein organism synthetic construct GGSISSSNFY 10 AA PAT source 1..10 mol_type protein organism synthetic construct GGSISSGGYY 8 AA PAT source 1..8 mol_type protein organism synthetic construct GGTFSNYA 8 AA PAT source 1..8 mol_type protein organism synthetic construct GYNFTFYW 8 AA PAT source 1..8 mol_type protein organism synthetic construct GFIFSDFG 8 AA PAT source 1..8 mol_type protein organism synthetic construct GFTFSNYG 8 AA PAT source 1..8 mol_type protein organism synthetic construct GFTFNTYD 8 AA PAT source 1..8 mol_type protein organism synthetic construct IVVGSGNT 8 AA PAT source 1..8 mol_type protein organism synthetic construct ISYDGSSK 7 AA PAT source 1..7 mol_type protein organism synthetic construct ITYSGRT 7 AA PAT source 1..7 mol_type protein organism synthetic construct IYYSGST 8 AA PAT source 1..8 mol_type protein organism synthetic construct FIRIFDTA 7 AA PAT source 1..7 mol_type protein organism synthetic construct IYPGDSE 7 AA PAT source 1..7 mol_type protein organism synthetic construct ISYDGSN 8 AA PAT source 1..8 mol_type protein organism synthetic construct ISFDGSNR 8 AA PAT source 1..8 mol_type protein organism synthetic construct IWYDGSNK 16 AA PAT source 1..16 mol_type protein organism synthetic construct AAPYCNSTNCYDAFNI 13 AA PAT source 1..13 mol_type protein organism synthetic construct VKDGEQLVPLFDY 22 AA PAT source 1..22 mol_type protein organism synthetic construct ARTFPSYYDRSGYHYLNYGMDV 21 AA PAT source 1..21 mol_type protein organism synthetic construct ASSNYYDGSGYNLLESYGMDV 17 AA PAT source 1..17 mol_type protein organism synthetic construct ARSPYYYDKSGYYYFDH 16 AA PAT source 1..16 mol_type protein organism synthetic construct TARLSGVQTALEYFDF 19 AA PAT source 1..19 mol_type protein organism synthetic construct KARDYYDISGFYHPPNFDY 7 AA PAT source 1..7 mol_type protein organism synthetic construct QSVSSSY 6 AA PAT source 1..6 mol_type protein organism synthetic construct QSISSY 8 AA PAT source 1..8 mol_type protein organism synthetic construct SGSIASNY 9 AA PAT source 1..9 mol_type protein organism synthetic construct SSNIGAGYD 6 AA PAT source 1..6 mol_type protein organism synthetic construct QNISSY 6 AA PAT source 1..6 mol_type protein organism synthetic construct QDITNH 6 AA PAT source 1..6 mol_type protein organism synthetic construct TLPKQY 6 AA PAT source 1..6 mol_type protein organism synthetic construct KLGDKY 11 AA PAT source 1..11 mol_type protein organism synthetic construct QSLLHSDGKTY 9 AA PAT source 1..9 mol_type protein organism synthetic construct QQYGRSPWT 10 AA PAT source 1..10 mol_type protein organism synthetic construct QQSYSTPGVT 9 AA PAT source 1..9 mol_type protein organism synthetic construct QSYDSSSWV 12 AA PAT source 1..12 mol_type protein organism synthetic construct QSYDSSLSGSWV 9 AA PAT source 1..9 mol_type protein organism synthetic construct QQSYSPQWT 9 AA PAT source 1..9 mol_type protein organism synthetic construct QQYDNLLLT 11 AA PAT source 1..11 mol_type protein organism synthetic construct QSADSSGTYWV