B7H3 mAb SHANGHAI HENLIUS BIOTECH, INC. Jen-Kuan Chang ANTI-B7H3 ANTIBODIES AND METHODS OF USE 84 8 AA PAT source 1..8 mol_type protein note 5H8 CDR1 organism synthetic construct GRTFSSDS 8 AA PAT source 1..8 mol_type protein note 5H8 CDR2 organism synthetic construct VSRSAGST 19 AA PAT source 1..19 mol_type protein note 5H8 CDR3 organism synthetic construct AATDIATMPWLMVSSRYDY 126 AA PAT source 1..126 mol_type protein note 5H8 VHH organism synthetic construct QVKLEESGGGQVQAGDSRTLSCVASGRTFSSDSMAWFREAPGKARGFVAVVSRSAGSTWYEDSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAATDIATMPWLMVSSRYDYWGQGTQVTVSS 8 AA PAT source 1..8 mol_type protein note 6H4 CDR1 organism synthetic construct GGSFSSDS 7 AA PAT source 1..7 mol_type protein note 6H4 CDR2 organism synthetic construct VTSAGST 19 AA PAT source 1..19 mol_type protein note 6H4 CDR3 organism synthetic construct AATTIATMPWLMVSSRYAY 125 AA PAT source 1..125 mol_type protein note 6H4 VHH organism synthetic construct QVQLQESGGDLVQPGGSLTLSCAASGGSFSSDSMGWFRQAPRKARGFVGAVTSAGSTWYEDSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAATTIATMPWLMVSSRYAYWGQGTQVTVSS 8 AA PAT source 1..8 mol_type protein note 7F3 CDR1 organism synthetic construct GRPFNSWS 7 AA PAT source 1..7 mol_type protein note 7F3 CDR2 organism synthetic construct VTSAGST 19 AA PAT source 1..19 mol_type protein note 7F3 CDR3 organism synthetic construct AATTIATMPWLMVSSRYAY 125 AA PAT source 1..125 mol_type protein note 7F3 VHH organism synthetic construct QVQLVDSGGGLVQAGDSLRLSCAASGRPFNSWSMGWFRQAPRKARGFVGAVTSAGSTWYEDSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAATTIATMPWLMVSSRYAYWGQGTQVTVSS 8 AA PAT source 1..8 mol_type protein note 7H7 CDR1 organism synthetic construct GSTFSKYA 7 AA PAT source 1..7 mol_type protein note 7H7 CDR2 organism synthetic construct VTSAGST 19 AA PAT source 1..19 mol_type protein note 7H7 CDR3 organism synthetic construct AATTIATMPWLMVSSRYAY 125 AA PAT source 1..125 mol_type protein note 7H7 VHH organism synthetic construct QVKLEESGGGLVQAGGSLRLSCAASGSTFSKYAMGWFRQAPRKARGFVGAVTSAGSTWYEDSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAATTIATMPWLMVSSRYAYWGQGTQVTVSS 8 AA PAT source 1..8 mol_type protein note 14B12 CDR1 organism synthetic construct GRTFRNFG 7 AA PAT source 1..7 mol_type protein note 14B12 CDR2 organism synthetic construct VTSAGST 19 AA PAT source 1..19 mol_type protein note 14B12 CDR3 organism synthetic construct AATTIATMPWLMVSSRYAY 125 AA PAT source 1..125 mol_type protein note 14B12 VHH organism synthetic construct QVQLVESGGGVVQAGGSLRLSCAASGRTFRNFGMGWFRQAPRKARGFVGAVTSAGSTWYEDSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAATTIATMPWLMVSSRYAYWGQGTQVTVSS 8 AA PAT source 1..8 mol_type protein note 14C4 CDR1 organism synthetic construct GRTFSSYG 7 AA PAT source 1..7 mol_type protein note 14C4 CDR2 organism synthetic construct VTSAGST 19 AA PAT source 1..19 mol_type protein note 14C4 CDR3 organism synthetic construct AATTIATMPWLMVSSRYAY 125 AA PAT source 1..125 mol_type protein note 14C4 VHH organism synthetic construct QVQLVESGGGVVQAGGSLRLSCAASGRTFSSYGMGWFRQAPRKARGFVGAVTSAGSTWYEDSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAATTIATMPWLMVSSRYAYWGQGTQVTVSS 8 AA PAT source 1..8 mol_type protein note 18B5 CDR1 organism synthetic construct GITFSSDS 8 AA PAT source 1..8 mol_type protein note 18B5 CDR2 organism synthetic construct VSRSAGST 19 AA PAT source 1..19 mol_type protein note 18B5 CDR3 organism synthetic construct AATDIATMPWLMVSSRYDY 126 AA PAT source 1..126 mol_type protein note 18B5 VHH organism synthetic construct QVQLQESGGGLVQAGGSLTLSCTASGITFSSDSMAWFREAPGKARGFVAVVSRSAGSTWYEDSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAATDIATMPWLMVSSRYDYWGQGTQVTVSS 7 AA PAT source 1..7 mol_type protein note 20C11 CDR1 organism synthetic construct ELTLDYN 7 AA PAT source 1..7 mol_type protein note 20C11 CDR2 organism synthetic construct VTSAGST 19 AA PAT source 1..19 mol_type protein note 20C11 CDR3 organism synthetic construct AATTIATMPWLMVSSRYAY 124 AA PAT source 1..124 mol_type protein note 20C11 VHH organism synthetic construct QVQLVESGGGLVQPGGSLRLSCAASELTLDYNIGWFRQAPRKARGFVGAVTSAGSTWYEDSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAATTIATMPWLMVSSRYAYWGQGTQVTVSS 12 AA PAT source 1..12 mol_type protein note 21F11 CDR1 organism synthetic construct GDDFFTINAIDA 7 AA PAT source 1..7 mol_type protein note 21F11 CDR2 organism synthetic construct VTSAGST 19 AA PAT source 1..19 mol_type protein note 21F11 CDR3 organism synthetic construct AATTIATMPWLMVSSRYAY 129 AA PAT source 1..129 mol_type protein note 21F11 VHH organism synthetic construct QVQLVESGGGLVQPGGSLRLSCAASGDDFFTINAIDAIGWFRQAPRKARGFVGAVTSAGSTWYEDSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAATTIATMPWLMVSSRYAYWGQGTQVTVSS 8 AA PAT source 1..8 mol_type protein note 5E8 CDR1 organism synthetic construct GRTFSSDS 8 AA PAT source 1..8 mol_type protein note 5E8 CDR2 organism synthetic construct VSRSAGST 19 AA PAT source 1..19 mol_type protein note 5E8 CDR3 organism synthetic construct AATDIATMPWLMVSSRYDY 126 AA PAT source 1..126 mol_type protein note 5E8 VHH organism synthetic construct QVKLEESGGGLVQAGGSLKLSCAASGRTFSSDSMAWFREAPGKARGFVAVVSRSAGSTWYEDSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAATDIATMPWLMVSSRYDYWGQGTQVTVSS 8 AA PAT source 1..8 mol_type protein note 19C10 CDR1 organism synthetic construct GSTFSKYA 7 AA PAT source 1..7 mol_type protein note 19C10 CDR2 organism synthetic construct VTSAGST 19 AA PAT source 1..19 mol_type protein note 19C10 CDR3 organism synthetic construct AATTIATMPWLMVSSRYAY 125 AA PAT source 1..125 mol_type protein note 19C10 VHH organism synthetic construct QVQLVESGGGLVQAGGSLRLSCAASGSTFSKYAMGWFRQAPRKARGFVGAVTSAGSTWYEDSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAATTIATMPWLMVSSRYAYWGQGTQVTVSS 8 AA PAT source 1..8 mol_type protein note 19C10-V2 CDR1 organism synthetic construct GSTFSKYA 7 AA PAT source 1..7 mol_type protein note 19C10-V2 CDR2 organism synthetic construct VTSAGST 19 AA PAT source 1..19 mol_type protein note 19C10-V2 CDR3 organism synthetic construct AATTIATMPWLMVSSRYAY 125 AA PAT source 1..125 mol_type protein note 19C10-V2 VHH organism synthetic construct EVQLVESGGGLVQPGGSLRLSCAASGSTFSKYAMGWFRQAPRKARGFVGAVTSAGSTWYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAATTIATMPWLMVSSRYAYWGQGTLVTVSS 8 AA PAT source 1..8 mol_type protein note 19C10-V3 CDR1 organism synthetic construct GSTFSKYA 7 AA PAT source 1..7 mol_type protein note 19C10-V3 CDR2 organism synthetic construct VTSAGST 19 AA PAT source 1..19 mol_type protein note 19C10-V3 CDR3 organism synthetic construct AATTIATMPWLMVSSRYAY 125 AA PAT source 1..125 mol_type protein note 19C10-V3 VHH organism synthetic construct EVQLVESGGGLVQPGGSLRLSCAASGSTFSKYAMGWFRQAPGKARGFVGAVTSAGSTWYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAATTIATMPWLMVSSRYAYWGQGTLVTVSS 357 AA PAT source 1..357 mol_type protein note 19C10 VHH-Fc organism synthetic construct QVQLVESGGGLVQAGGSLRLSCAASGSTFSKYAMGWFRQAPRKARGFVGAVTSAGSTWYEDSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAATTIATMPWLMVSSRYAYWGQGTQVTVSSEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 357 AA PAT source 1..357 mol_type protein note 19C10 VHH-Fc 2 organism synthetic construct QVQLVESGGGLVQAGGSLRLSCAASGSTFSKYAMGWFRQAPRKARGFVGAVTSAGSTWYEDSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAATTIATMPWLMVSSRYAYWGQGTQVTVSSEPKSCDKTHTCPPCPAPELVGGPSVFLLPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPPEEQYNSTLRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 357 AA PAT source 1..357 mol_type protein note 19C10-V3 VHH-Fc 2 organism synthetic construct EVQLVESGGGLVQPGGSLRLSCAASGSTFSKYAMGWFRQAPGKARGFVGAVTSAGSTWYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAATTIATMPWLMVSSRYAYWGQGTLVTVSSEPKSCDKTHTCPPCPAPELVGGPSVFLLPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPPEEQYNSTLRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 8 AA PAT source 1..8 mol_type protein note 19C10-V4 CDR1 organism synthetic construct GSTFSKYA 7 AA PAT source 1..7 mol_type protein note 19C10-V4 CDR2 organism synthetic construct VTSAGST 19 AA PAT source 1..19 mol_type protein note 19C10-V4 CDR3 organism synthetic construct AATTIATMPWLMVSSRYAY 125 AA PAT source 1..125 mol_type protein note 19C10-V4 VHH organism synthetic construct EVQLLESGGGLVQPGGSLRLSCAASGSTFSKYAMGWFRQAPGKARGFVGAVTSAGSTWYEDSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAATTIATMPWLMVSSRYAYWGQGTLVTVSS 357 AA PAT source 1..357 mol_type protein note 19C10-V4 VHH-Fc organism synthetic construct EVQLLESGGGLVQPGGSLRLSCAASGSTFSKYAMGWFRQAPGKARGFVGAVTSAGSTWYEDSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAATTIATMPWLMVSSRYAYWGQGTLVTVSSEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 12 AA PAT source 1..12 mol_type protein note Exemplary linker61 organism synthetic construct GSGGSGGSGGSG 15 AA PAT source 1..15 mol_type protein note Exemplary linker62 organism synthetic construct GGGGSGGGGSGGGGS 5 AA PAT source 1..5 mol_type protein note Exemplary linker63 organism synthetic construct GGGSG 10 AA PAT source 1..10 mol_type protein note Exemplary linker64 organism synthetic construct GGGSGGGGSG 8 AA PAT source 1..8 mol_type protein note Exemplary linker65 organism synthetic construct GGSGGGSG 12 AA PAT source 1..12 mol_type protein note Exemplary linker66 organism synthetic construct GGSGGGSGGGSG 6 AA PAT source 1..6 mol_type protein note Exemplary linker67 organism synthetic construct GSGGSG 9 AA PAT source 1..9 mol_type protein note Exemplary linker68 organism synthetic construct GSGGSGGSG 7 AA PAT source 1..7 mol_type protein note Exemplary linker69 organism synthetic construct GSGSGSG 18 AA PAT source 1..18 mol_type protein note Exemplary linker70 organism synthetic construct GGGGSGGGGSGGGGSGGG 5 AA PAT source 1..5 mol_type protein note Exemplary linker71 organism synthetic construct PAPAP 7 AA PAT source 1..7 mol_type protein note Exemplary linker72 organism synthetic construct IKRTVAA 7 AA PAT source 1..7 mol_type protein note Exemplary linker73 organism synthetic construct VSSASTK 4 AA PAT source 1..4 mol_type protein note Exemplary linker74 organism synthetic construct ASTK 12 AA PAT source 1..12 mol_type protein note Exemplary linker75 organism synthetic construct ASTKSGGSGGSG 7 AA PAT source 1..7 mol_type protein note Exemplary linker76 organism synthetic construct AEAAAKA 12 AA PAT source 1..12 mol_type protein note Exemplary linker77 organism synthetic construct AEAAAKEAAAKA 10 AA PAT source 1..10 mol_type protein note Exemplary linker78 organism synthetic construct GRPGSGRPGS 10 AA PAT source 1..10 mol_type protein note Exemplary linker79 organism synthetic construct GRGGSGRGGS 10 AA PAT source 1..10 mol_type protein note Exemplary linker80 organism synthetic construct GKPGSGKPGS 10 AA PAT source 1..10 mol_type protein note Exemplary linker81 organism synthetic construct GEPGSGEPGS 10 AA PAT source 1..10 mol_type protein note Exemplary linker82 organism synthetic construct GDPGSGDPGS 534 AA PAT source 1..534 mol_type protein note Human B7H3 polypeptide organism synthetic construct MLRRRGSPGMGVHVGAALGALWFCLTGALEVQVPEDPVVALVGTDATLCCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFAEGQDQGSAYANRTALFPDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYQGYPEAEVFWQDGQGVPLTGNVTTSQMANEQGLFDVHSILRVVLGANGTYSCLVRNPVLQQDAHSSVTITPQRSPTGAVEVQVPEDPVVALVGTDATLRCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFTEGRDQGSAYANRTALFPDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYRGYPEAEVFWQDGQGVPLTGNVTTSQMANEQGLFDVHSVLRVVLGANGTYSCLVRNPVLQQDAHGSVTITGQPMTFPPEALWVTVGLSVCLIALLVALAFVCWRKIKQSCEEENAGAEDQDGEGEGSKTALQPLKHSDSKEDDGQEIA 461 AA PAT source 1..461 mol_type protein note ECD of Human B7H3 polypeptide organism synthetic construct MLRRRGSPGMGVHVGAALGALWFCLTGALEVQVPEDPVVALVGTDATLCCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFAEGQDQGSAYANRTALFPDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYQGYPEAEVFWQDGQGVPLTGNVTTSQMANEQGLFDVHSILRVVLGANGTYSCLVRNPVLQQDAHSSVTITPQRSPTGAVEVQVPEDPVVALVGTDATLRCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFTEGRDQGSAYANRTALFPDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYRGYPEAEVFWQDGQGVPLTGNVTTSQMANEQGLFDVHSVLRVVLGANGTYSCLVRNPVLQQDAHGSVTITGQPMT