82843PC01 EP EP23218475.4 2023-12-20 Danmarks Tekniske Universitet Method for enzyme fitness data collection 16 3206 DNA PAT source 1..3206 mol_type other DNA organism synthetic construct attcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatttgacttttgtccctttttttttggacgacccttgtccgcgcgttgtccttttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatccatcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactcatatccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatccatcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccgggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctcaacgggaatcctgctctgcgaggctggccgtaggccggcctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttatgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacatgtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgcggcgcgcccagctgtctagggcggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctttaattaagcggccgcggcgcgcccgtcgacgcgcgcgcggctgaggatacctcagctactaaaagccagataacagtatgcgtatttgcgcgctgaatagttgacggctagctcagtcctaggtacagtgcaatcgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctgcggagggtttctaggagaccggatccgtcgacctgcaggcatgcaagcttactagtagatctggtctcggacttcgcactcactgcttcacacactagtcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaggggtccccaataattacgatttaaattggcgaaaatgagacgttgatcggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcac 1092 DNA PAT source 1..1092 mol_type other DNA organism synthetic construct atgggtagcaccgcagaaacccagctgacaccggttcaggttaccgatgatgaagcagcactgtttgcaatgcagctggcaagcgcaagcgttctgccgatggcactgaaaagcgcactggaactggatctgctggaaattatggcaaaaaatggtagcccgatgagcccgaccgaaattgcaagcaaactgccgaccaaaaatccggaagcaccggttatgctggatcgtattctgcgtctgctgaccagctatagcgttctgacctgtagcaatcgtaaactgagcggtgatggtgttgaacgtatttatggtctgggtccggtttgtaaatatctgaccaaaaacgaagatggtgttagcattgcagccctgtgtctgatgaatcaggataaagttctgatggaaagctggtatcatctgaaagatgcaattctggatggtggcattccgtttaacaaagcatatggtatgagcgcctttgaatatcatggcaccgatccgcgtttcaacaaagtttttaacaatggcatgagcaaccacagcaccatcacaatgaaaaaaatcctggaaacctacaaaggctttgaaggtctgaccagcctggttgatgttggtggtggtattggtgcaaccctgaaaatgattgttagcaaatatccgaacctgaaaggcatcaattttgatctgccgcatgttattgaagatgcaccgagccatccgggtattgaacatgtgggtggtgatatgtttgttagcgttccgaaaggtgatgccatctttatgaaatggatttgccatgattggagcgacgaacattgtgtgaaatttctgaaaaactgctatgaaagcctgccggaagatggtaaagttattctggcagaatgtatcctgcctgaaacaccggatagcagcctgagcaccaaacaggttgttcatgttgattgtattatgctggcacataatccgggtggtaaagaacgtaccgaaaaagaatttgaagcactggcaaaagccagcggttttaaaggtattaaagttgtgtgtgatgcgtttggcgtgaatctgattgaactgctgaaaaaactgtaa 363 AA PAT source 1..363 mol_type protein organism synthetic construct MGSTAETQLTPVQVTDDEAALFAMQLASASVLPMALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNRKLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDGGIPFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKGFEGLTSLVDVGGGIGATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYESLPEDGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERTEKEFEALAKASGFKGIKVVCDAFGVNLIELLKKL 34 DNA PAT source 1..34 mol_type other DNA organism synthetic construct gaaggtctcttctaatgggtagcaccgcagaaac 31 DNA PAT source 1..31 mol_type other DNA organism synthetic construct ttacagttttttcagcagttcaatcagattc 91 DNA PAT source 1..91 mol_type other DNA organism synthetic construct gaaggtctctagtcaccgaagtgaggatgttgcctnnnnnnnnnnnnnnnnnnnnnnnnnttacagttttttcagcagttcaatcagattc 4288 DNA PAT source 1..4288 mol_type other DNA organism synthetic construct attcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatttgacttttgtccctttttttttggacgacccttgtccgcgcgttgtccttttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatccatcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactcatatccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatccatcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccgggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctcaacgggaatcctgctctgcgaggctggccgtaggccggcctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttatgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacatgtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgcggcgcgcccagctgtctagggcggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctttaattaagcggccgcggcgcgcccgtcgacgcgcgcgcggctgaggatacctcagctactaaaagccagataacagtatgcgtatttgcgcgctgaatagttgacggctagctcagtcctaggtacagtgcaatcgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctgcggagggtttctaatgggtagcaccgcagaaacccagctgacaccggttcaggttaccgatgatgaagcagcactgtttgcaatgcagctggcaagcgcaagcgttctgccgatggcactgaaaagcgcactggaactggatctgctggaaattatggcaaaaaatggtagcccgatgagcccgaccgaaattgcaagcaaactgccgaccaaaaatccggaagcaccggttatgctggatcgtattctgcgtctgctgaccagctatagcgttctgacctgtagcaatcgtaaactgagcggtgatggtgttgaacgtatttatggtctgggtccggtttgtaaatatctgaccaaaaacgaagatggtgttagcattgcagccctgtgtctgatgaatcaggataaagttctgatggaaagctggtatcatctgaaagatgcaattctggatggtggcattccgtttaacaaagcatatggtatgagcgcctttgaatatcatggcaccgatccgcgtttcaacaaagtttttaacaatggcatgagcaaccacagcaccatcacaatgaaaaaaatcctggaaacctacaaaggctttgaaggtctgaccagcctggttgatgttggtggtggtattggtgcaaccctgaaaatgattgttagcaaatatccgaacctgaaaggcatcaattttgatctgccgcatgttattgaagatgcaccgagccatccgggtattgaacatgtgggtggtgatatgtttgttagcgttccgaaaggtgatgccatctttatgaaatggatttgccatgattggagcgacgaacattgtgtgaaatttctgaaaaactgctatgaaagcctgccggaagatggtaaagttattctggcagaatgtatcctgcctgaaacaccggatagcagcctgagcaccaaacaggttgttcatgttgattgtattatgctggcacataatccgggtggtaaagaacgtaccgaaaaagaatttgaagcactggcaaaagccagcggttttaaaggtattaaagttgtgtgtgatgcgtttggcgtgaatctgattgaactgctgaaaaaactgtaannnnnnnnnnnnnnnnnnnnnnnnnaggcaacatcctcacttcggtgacttcgcactcactgcttcacacactagtcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaggggtccccaataattacgatttaaattggcgaaaatgagacgttgatcggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcac 31 DNA PAT source 1..31 mol_type other DNA organism synthetic construct gaatctgattgaactgctgaaaaaactgtaa 25 DNA PAT source 1..25 mol_type other DNA organism synthetic construct gcttccttagctcctgaaaatctcg 363 AA PAT source 1..363 mol_type protein organism synthetic construct MGSTAETQLTPVQVTDDEAALFAMQLASASVLPMALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEASVMLDRILRLLTSYSVLTCSNRKLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDRVLMESWYHLKDAILEGGIPFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKGFEGLTSLVDVGGGIGATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYESLPEDGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERTEKEFEALAKASGFKGIKVVCDAFGVNLIELLKKL 363 AA PAT source 1..363 mol_type protein organism synthetic construct MGSTAETQLTPVQVTDDEAALFAMQLASASVLPMALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEASVMLDRILRLLTSYSVLTCSNRKLSGDGVERNYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDGGIPFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKGFEGLTSLVDVGGGIGATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYESLLEDGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERTEKEFEALAKASGFKGIKVVCDAFGVNLIELLKKL 363 AA PAT source 1..363 mol_type protein organism synthetic construct MGSTAETQLTPVQVTDDEAALFAMQLASASVLPMALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDCILRLLTSYSVLTCSNRKLSGDGVERIYSLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDGGIPFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMRKILETYKGFEGLTSLVDVGGGIGATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYESLPEDGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERTEKEFEALAKASGFKGIKVVCDAFGVNLIELLKKL 363 AA PAT source 1..363 mol_type protein organism synthetic construct MGSTAETQLTPVQVTDDEAALFAMQLASASVLPMALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDCILRLLTSYSVLTCSNRKLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDGGIPFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKGFEGLTSLVDVGGGIGATLKKIVSKYPNLKGINFDLSHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYESLPEDGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERTEKEFEALAKASGFKGIKVVCDAFGVNLIELLKKL 363 AA PAT source 1..363 mol_type protein organism synthetic construct MGSTAETQLTPVQVTDDEAALFAMQLASASVLPMALKSALELDLLDIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTIYSVLTCSNRKLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDGGIPFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKGFEGLTSLVDVGGGIGATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYESLPEDGKVILAECILPETLDSSLSTKQVVHVDCIMLAHNPGGKERTEKEFEALAKASGFKGIKVVCDAFGVNLIELLKKL 31 DNA PAT source 1..31 mol_type other DNA organism synthetic construct ttgacggctagctcagtcctaggtacagtgc 31 DNA PAT source 1..31 mol_type other DNA organism synthetic construct tttacggctagctcagtcctaggtacaatgc