072 CN 202211521035.0 2022-11-30 SHANGHAI CHIA TAI TIANQING PHARMACEUTICAL TECHNOLOGY DEVELOPMENT CO., LTD. ANTI-CLDN18.2 ANTIBODY-DRUG CONJUGATE, AND PHARMACEUTICAL COMPOSITION THEREOF AND USE THEREOF 71 330 AA PAT source 1..330 mol_type protein note Heavy chain constant region of chimeric and humanized antibodies organism synthetic construct ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 107 AA PAT source 1..107 mol_type protein note Light chain constant region of chimeric and humanized antibodies organism synthetic construct RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC 5 AA PAT source 1..5 mol_type protein note HCDR1 of mouse, chimeric, and humanized 28G3 organism synthetic construct DYLML 17 AA PAT source 1..17 mol_type protein note HCDR2 of mouse and chimeric 28G3 organism synthetic construct NINPYYGRTTYNLKFKG 17 AA PAT source 1..17 mol_type protein note HCDR2 of hz28G3-1.1, hz28G3-1.3, hz28G3-2.1, and hz28G3-2.3 organism synthetic construct NINPYYGRTTYALKFKG 17 AA PAT source 1..17 mol_type protein note HCDR2 of hz28G3-1.2, hz28G3-1.4, hz28G3-2.2, and hz28G3-2.4 organism synthetic construct NINPYYGRTYYALKFQG 11 AA PAT source 1..11 mol_type protein note HCDR3 of mouse, chimeric, and humanized 28G3 organism synthetic construct DGYSLRNAMDY 17 AA PAT source 1..17 mol_type protein note LCDR1 of mouse, chimeric, and humanized 28G3 organism synthetic construct KSSQSLFNSGNQKNYLA 7 AA PAT source 1..7 mol_type protein note LCDR2 of mouse, chimeric, and humanized 28G3 organism synthetic construct GASTRES 9 AA PAT source 1..9 mol_type protein note LCDR3 of mouse, chimeric, and humanized 28G3 organism synthetic construct QNAHSYPYT 120 AA PAT source 1..120 mol_type protein note VH of mouse and chimeric 28G3 organism synthetic construct EIQLQQTGPELVKPGASVTISCKTSGYSFTDYLMLWVKQSHGKSLEWIGNINPYYGRTTYNLKFKGKATLTVDRSSSTAYMQLNSLTSEDSAVYYCARDGYSLRNAMDYWGQGTSVTVSS 120 AA PAT source 1..120 mol_type protein note VH of hz28G3-1.1 and hz28G3-2.1 organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKTSGYSFTDYLMLWVRQAPGQGLEWMGNINPYYGRTTYALKFKGRVTMTVDRSTSTVYMELSSLRSEDTAVYYCARDGYSLRNAMDYWGQGTLVTVSS 120 AA PAT source 1..120 mol_type protein note VH of hz28G3-1.2 and hz28G3-2.2 organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTDYLMLWVRQAPGQGLEWMGNINPYYGRTYYALKFQGRVTMTVDRSTSTVYMELSSLRSEDTAVYYCARDGYSLRNAMDYWGQGTLVTVSS 120 AA PAT source 1..120 mol_type protein note VH of hz28G3-1.3 and hz28G3-2.3 organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKTSGYSFTDYLMLWVRQAHGQSLEWMGNINPYYGRTTYALKFKGRVTMTVDRSTSTVYMELSSLRSEDTAVYYCARDGYSLRNAMDYWGQGTLVTVSS 120 AA PAT source 1..120 mol_type protein note VH of hz28G3-1.4 and hz28G3-2.4 organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTDYLMLWVRQAHGQSLEWMGNINPYYGRTYYALKFQGRVTMTVDRSTSTVYMELSSLRSEDTAVYYCARDGYSLRNAMDYWGQGTLVTVSS 113 AA PAT source 1..113 mol_type protein note VL of mouse and chimeric 28G3 organism synthetic construct DIVMTQSPYSLSVSTGEKVTMSCKSSQSLFNSGNQKNYLAWYQQKPGQPPKLLIYGASTRESGVPDRFTGSGSGTDFTLTISSVQAEDLALYYCQNAHSYPYTFGGGTKLEIK 113 AA PAT source 1..113 mol_type protein note VL of hz28G3-1.1, hz28G3-1.2, hz28G3-1.3, and hz28G3-1.4 organism synthetic construct DIVMTQSPDSLAVSLGERATINCKSSQSLFNSGNQKNYLAWYQQKPGQPPKLLIYGASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQNAHSYPYTFGQGTKVEIK 113 AA PAT source 1..113 mol_type protein note VL of hz28G3-2.1, hz28G3-2.2, hz28G3-2.3, and hz28G3-2.4 organism synthetic construct DIVMTQSPYSLAVSLGERATINCKSSQSLFNSGNQKNYLAWYQQKPGQPPKLLIYGASTRESGVPDRFTGSGSGTDFTLTISSLQAEDVAVYYCQNAHSYPYTFGQGTKVEIK 5 AA PAT source 1..5 mol_type protein note HCDR1 of mouse, chimeric, and humanized 40F6 organism synthetic construct SYGVY 16 AA PAT source 1..16 mol_type protein note HCDR2 of mouse and chimeric 40F6, hz40F6-1.2, and hz40F6-2.2 organism synthetic construct VVWAGGNTNYNSALMS 16 AA PAT source 1..16 mol_type protein note HCDR2 of hz40F6-1.1 and hz40F6-2.1 organism synthetic construct VVWAGGNTNYADSVKG 16 AA PAT source 1..16 mol_type protein note HCDR2 of hz40F6-1.3 and hz40F6-2.3 organism synthetic construct VVWAGGNTNYADALKG 16 AA PAT source 1..16 mol_type protein note HCDR2 of hz40F6-1.4 and hz40F6-2.4 organism synthetic construct VVWAGGNTNYNSALKG 10 AA PAT source 1..10 mol_type protein note HCDR3 of mouse, chimeric, and humanized 40F6 organism synthetic construct DRGRLLAMDY 17 AA PAT source 1..17 mol_type protein note LCDR1 of mouse, chimeric, and humanized 40F6 organism synthetic construct KSSQSLLNSGNQKNYLA 7 AA PAT source 1..7 mol_type protein note LCDR2 of mouse, chimeric, and humanized 40F6 organism synthetic construct GASTRES 9 AA PAT source 1..9 mol_type protein note LCDR3 of mouse and chimeric 40F6, hz40F6-1.1, hz40F6-1.2, hz40F6-1.3, and hz40F6-1.4 organism synthetic construct QNDHFFPFT 9 AA PAT source 1..9 mol_type protein note LCDR3 of hz40F6-2.1, hz40F6-2.2, hz40F6-2.3, and hz40F6-2.4 organism synthetic construct QNDYFFPFT 118 AA PAT source 1..118 mol_type protein note VH of mouse and chimeric 40F6 organism synthetic construct QVQLKESGPGLVAPSQSLSITCTVSGFSLTSYGVYWVRQSPGKGLEWLGVVWAGGNTNYNSALMSRLSISKDNSKSQVFLKMNSLQTDDTAMYYCARDRGRLLAMDYWGQGTSVTVSS 118 AA PAT source 1..118 mol_type protein note VH of hz40F6-1.1 and hz40F6-2.1 organism synthetic construct QVQLVESGGGVVQPGRSLRLSCAASGFTLTSYGVYWVRQAPGKGLEWVAVVWAGGNTNYADSVKGRFTISKDNSKSTLYLQMNSLRAEDTAVYYCARDRGRLLAMDYWGQGTLVTVSS 118 AA PAT source 1..118 mol_type protein note VH of hz40F6-1.2 and hz40F6-2.2 organism synthetic construct QVQLVESGGGVVQPGRSLRLSCAASGFTLTSYGVYWVRQAPGKGLEWVAVVWAGGNTNYNSALMSRFTISKDNSKSTLYLQMNSLRAEDTAVYYCARDRGRLLAMDYWGQGTLVTVSS 118 AA PAT source 1..118 mol_type protein note VH of hz40F6-1.3 and hz40F6-2.3 organism synthetic construct QVQLVESGGGVVQPGRSLRLSCAASGFTLTSYGVYWVRQAPGKGLEWVAVVWAGGNTNYADALKGRFTISKDNSKSTLYLQMNSLRAEDTAVYYCARDRGRLLAMDYWGQGTLVTVSS 118 AA PAT source 1..118 mol_type protein note VH of hz40F6-1.4 and hz40F6-2.4 organism synthetic construct QVQLVESGGGVVQPGRSLRLSCTVSGFTLTSYGVYWVRQAPGKGLEWVAVVWAGGNTNYNSALKGRFTISKDNSKSTLYLQMNSLRAEDTAVYYCARDRGRLLAMDYWGQGTLVTVSS 113 AA PAT source 1..113 mol_type protein note VL of mouse and chimeric 40F6 organism synthetic construct DVVMTQSPSSLSVSAGEKVTMSCKSSQSLLNSGNQKNYLAWYQQKPGQPPKLLIYGASTRESGVPDRFTGSGSGTDFTLTISSVQAEDLAVYYCQNDHFFPFTFGSGTKLEIK 113 AA PAT source 1..113 mol_type protein note VL of hz40F6-1.1, hz40F6-1.2, hz40F6-1.3, and hz40F6-1.4 organism synthetic construct DIVMTQSPDSLAVSLGERATINCKSSQSLLNSGNQKNYLAWYQQKPGQPPKLLIYGASTRESGVPDRFTGSGSGTDFTLTISSLQAEDVAVYYCQNDHFFPFTFGQGTKVEIK 113 AA PAT source 1..113 mol_type protein note VL of hz40F6-2.1, hz40F6-2.2, hz40F6-2.3, and hz40F6-2.4 organism synthetic construct DIVMTQSPDSLAVSLGERATINCKSSQSLLNSGNQKNYLAWYQQKPGQPPKLLIYGASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQNDYFFPFTFGQGTKVEIK 5 AA PAT source 1..5 mol_type protein note HCDR1 of mouse and chimeric 34G6 organism synthetic construct SYGVH 16 AA PAT source 1..16 mol_type protein note HCDR2 of mouse and chimeric 34G6 organism synthetic construct VIWSDGSTTYNSALKS 10 AA PAT source 1..10 mol_type protein note HCDR3 of mouse and chimeric 34G6 organism synthetic construct HAYYGNSFDY 17 AA PAT source 1..17 mol_type protein note LCDR1 of mouse and chimeric 34G6 organism synthetic construct KSSQSLLNSGNQKSYLT 7 AA PAT source 1..7 mol_type protein note LCDR2 of mouse and chimeric 34G6 organism synthetic construct WASTRES 9 AA PAT source 1..9 mol_type protein note LCDR3 of mouse and chimeric 34G6 organism synthetic construct QNVYIFPLT 118 AA PAT source 1..118 mol_type protein note VH of mouse and chimeric 34G6 organism synthetic construct QVQLKESGPDLVAPSQSLSITCTVSGFSLTSYGVHWVRQPPGKGLEWLVVIWSDGSTTYNSALKSRLSISKDNSKSQVFLKMNSLQTDDTAIYYCARHAYYGNSFDYWGQGTTLTVSS 113 AA PAT source 1..113 mol_type protein note VL of mouse and chimeric 34G6 organism synthetic construct DIVMTQSPSSLTVTAGEKVTMSCKSSQSLLNSGNQKSYLTWYQQKPGQPPKLLIYWASTRESGVPDRFTGSGSGTDFTLTISSVQAEDLAVYYCQNVYIFPLTFGAGTKLELK 5 AA PAT source 1..5 mol_type protein note HCDR1 of mouse and chimeric 10D8 organism synthetic construct NYGMN 17 AA PAT source 1..17 mol_type protein note HCDR2 of mouse and chimeric 10D8 organism synthetic construct WININTGEPRYAEEFKG 9 AA PAT source 1..9 mol_type protein note HCDR3 of mouse and chimeric 10D8 organism synthetic construct YGYGNSFPY 17 AA PAT source 1..17 mol_type protein note LCDR1 of mouse and chimeric 10D8 organism synthetic construct KSSQSLLNSGNQRNYLT 7 AA PAT source 1..7 mol_type protein note LCDR2 of mouse and chimeric 10D8 organism synthetic construct WASTRES 9 AA PAT source 1..9 mol_type protein note LCDR3 of mouse and chimeric 10D8 organism synthetic construct QNDYSYPLT 118 AA PAT source 1..118 mol_type protein note VH of mouse and chimeric 10D8 organism synthetic construct QIQLAQSGPELKKPGETVKVSCKASGYTFKNYGMNWVKQAPGKGLKWMGWININTGEPRYAEEFKGRIAFSLETSASTAYLLINNLKNEDTATYFCARYGYGNSFPYWGQGTLVTVSA 113 AA PAT source 1..113 mol_type protein note VL of mouse and chimeric 10D8 organism synthetic construct DIVMTQSPSSLTVTVGEKVTMSCKSSQSLLNSGNQRNYLTWYQQKPGQPPKLLIYWASTRESGVPDRFTGSGSGTAFTLTVSSVQAEDLAVYYCQNDYSYPLTFGAGTKLEMK 5 AA PAT source 1..5 mol_type protein note HCDR1 of mouse and chimeric 22F12 organism synthetic construct SYGVY 16 AA PAT source 1..16 mol_type protein note HCDR2 of mouse and chimeric 22F12 organism synthetic construct VIWAGGSTNYNSALMS 10 AA PAT source 1..10 mol_type protein note HCDR3 of mouse and chimeric 22F12 organism synthetic construct DRGRLLSMDY 17 AA PAT source 1..17 mol_type protein note LCDR1 of mouse and chimeric 22F12 organism synthetic construct RSSQSLLNSGNQKNYLA 7 AA PAT source 1..7 mol_type protein note LCDR2 of mouse and chimeric 22F12 organism synthetic construct GASTRES 9 AA PAT source 1..9 mol_type protein note LCDR3 of mouse and chimeric 22F12 organism synthetic construct QNVHFFPFT 118 AA PAT source 1..118 mol_type protein note VH of mouse and chimeric 22F12 organism synthetic construct QVQLKESGPGLVAPSQSLSITCTVSGFSLTSYGVYWVRQPPGKGLEWLGVIWAGGSTNYNSALMSRLSINKDNSKSQVFLKMNSLQTDDTAMYYCARDRGRLLSMDYWGQGTSVTVSS 113 AA PAT source 1..113 mol_type protein note VL of mouse and chimeric 22F12 organism synthetic construct DIVMTQSPSSLSVSAGEKVTMSCRSSQSLLNSGNQKNYLAWYQQKPGQPPKLLIYGASTRESGVPDRFTGSGSGTDFTLTIGSVQAEDLAVYYCQNVHFFPFTFGSGTKLEIK 450 AA PAT source 1..450 mol_type protein note Heavy chain of hz28G3-1.1 organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKTSGYSFTDYLMLWVRQAPGQGLEWMGNINPYYGRTTYALKFKGRVTMTVDRSTSTVYMELSSLRSEDTAVYYCARDGYSLRNAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 220 AA PAT source 1..220 mol_type protein note Light chain of hz28G3-1.1 organism synthetic construct DIVMTQSPDSLAVSLGERATINCKSSQSLFNSGNQKNYLAWYQQKPGQPPKLLIYGASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQNAHSYPYTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC 450 AA PAT source 1..450 mol_type protein note Heavy chain of hz28G3-1.3 organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKTSGYSFTDYLMLWVRQAHGQSLEWMGNINPYYGRTTYALKFKGRVTMTVDRSTSTVYMELSSLRSEDTAVYYCARDGYSLRNAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 220 AA PAT source 1..220 mol_type protein note Light chain of hz28G3-1.3 organism synthetic construct DIVMTQSPDSLAVSLGERATINCKSSQSLFNSGNQKNYLAWYQQKPGQPPKLLIYGASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQNAHSYPYTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC 261 AA PAT source 1..261 mol_type protein organism Homo sapiens MAVTACQGLGFVVSLIGIAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV 261 AA PAT source 1..261 mol_type protein organism Homo sapiens MSTTTCQVVAFLLSILGLAGCIAATGMDMWSTQDLYDNPVTSVFQYEGLWRSCVRQSSGFTECRPYFTILGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV 448 AA PAT source 1..448 mol_type protein note Heavy chain of 15F9 organism synthetic construct QVQLVQSGSELKKPGASVKVSCKASGYTFTNYGINWVRQAPGQGLEWMGWINPNTGETTYAEEFKGRFVFSLDTSVSTAYLQISSLKAEDTAVYFCARLYYGNRFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 220 AA PAT source 1..220 mol_type protein note Light chain of 15F9 organism synthetic construct DIVMTQSPDSLAVSLGERATINCKSSQSLLNSGNQRNYLTWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQNAYYYPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC 17 AA PAT source 1..17 mol_type protein note HCDR2 of mouse, chimeric, and humanized 28G3 organism synthetic construct VARIANT 10 note X = T or Y VARIANT 12 note X = N or A VARIANT 16 note X = K or Q NINPYYGRTXYXLKFXG 16 AA PAT source 1..16 mol_type protein note HCDR2 of mouse, chimeric, and humanized 40F6 organism synthetic construct VARIANT 11 note X = N or A VARIANT 12 note X = S or D VARIANT 13 note X = A or S VARIANT 14 note X = L or V VARIANT 15 note X = M or K VARIANT 16 note X = S or G VVWAGGNTNYXXXXXX 9 AA PAT source 1..9 mol_type protein note LCDR3 of mouse, chimeric, and humanized 40F6 organism synthetic construct VARIANT 4 note X = H or Y QNDXFFPFT