P26410200SG CN 2024101349441 2024-01-31 SICHUAN HUIYU PHARMACEUTICAL CO., LTD. Xuan Zheng BISPECIFIC ANTIBODY-CYTOKINE FUSION PROTEIN AND USE THEREOF 79 119 AA PAT source 1..119 mol_type protein note Full-length sequence of VH of 3A03A03/7TI-042 organism synthetic construct EVQLQQSGGGLVKPGGSLKLSCAASGFTFSDYGMHWFRQAPEKGLEWVAYISSGSSTIYYADTVKGRFTISRDNAKDTLFLQMTSLRSEDTAMYYCARYRFDAYAMDYWGQGTTVTVSS 107 AA PAT source 1..107 mol_type protein note Full-length sequence of VL of 3A03A03/7TI-042 organism synthetic construct DIVMTQAASSLSASLGDRVTISCRASQDISNYLNWYQQKPDGAVKLLIYYKSRLHSGVPSRFSGSGSGTDYSLTISNLEPEDIATYYCQHFRKVPWTFGGGTKLEIK 10 AA PAT source 1..10 mol_type protein note 3A03A03/7TI-042 CDR-H1 organism synthetic construct GFTFSDYGMH 17 AA PAT source 1..17 mol_type protein note 3A03A03/7TI-042 CDR-H2 organism synthetic construct YISSGSSTIYYADTVKG 12 AA PAT source 1..12 mol_type protein note 3A03A03/7TI-042 CDR-H3 organism synthetic construct ARYRFDAYAMDY 11 AA PAT source 1..11 mol_type protein note 3A03A03/7TI-042 CDR-L1 organism synthetic construct RASQDISNYLN 7 AA PAT source 1..7 mol_type protein note 3A03A03/7TI-042 CDR-L2 organism synthetic construct YKSRLHS 9 AA PAT source 1..9 mol_type protein note 3A03A03/7TI-042 CDR-L3 organism synthetic construct QHFRKVPWT 118 AA PAT source 1..118 mol_type protein note Full-length sequence of VH of 2IT1-10A3-2H6/7TI-027 organism synthetic construct EVKLVESGAELLKPGASVKLSCKTSGYTFTSYWINWVKQRPGQGLEWIGNIYPALDSTNYNEKFKSKATLTVDTSSSTAYMQLNSLTSDDSAVYYCARLRQFGLFDQWGQGTTVTVSS 106 AA PAT source 1..106 mol_type protein note Full-length sequence of VL of 2IT1-10A3-2H6/7TI-027 organism synthetic construct DIVMTQSPTIMSASPGEKVTMTCSASSGVSYMHWYLQKSGTSPKLLIFDTSKLASGVPARFSGSGSGTSYSLTISSMEAEDAATYYCQQWSNNPLTFGGGTKLEIK 10 AA PAT source 1..10 mol_type protein note 2IT1-10A3-2H6/7TI-027 CDR-H1 organism synthetic construct GYTFTSYWIN 17 AA PAT source 1..17 mol_type protein note 2IT1-10A3-2H6/7TI-027 CDR-H2 organism synthetic construct NIYPALDSTNYNEKFKS 11 AA PAT source 1..11 mol_type protein note 2IT1-10A3-2H6/7TI-027 CDR-H3 organism synthetic construct ARLRQFGLFDQ 10 AA PAT source 1..10 mol_type protein note 2IT1-10A3-2H6/7TI-027 CDR-L1 organism synthetic construct SASSGVSYMH 7 AA PAT source 1..7 mol_type protein note 2IT1-10A3-2H6/7TI-027 CDR-L2 organism synthetic construct DTSKLAS 9 AA PAT source 1..9 mol_type protein note 2IT1-10A3-2H6/7TI-027 CDR-L3 organism synthetic construct QQWSNNPLT 119 AA PAT source 1..119 mol_type protein note Full-length sequence of VH of 41A07A03/7TI-046 organism synthetic construct EVQLKESGGGLVKPGGSLKLSCAASGFTFSSYAMSWVRQTPERRLEWVAFISSGSSTIFYADTVEGRFTISRDNANNTLFLQMTSLRSEDTAMYYCARRSLDYYAMDYWGQGTTVTVSS 107 AA PAT source 1..107 mol_type protein note Full-length sequence of VL of 41A07A03/7TI-046 organism synthetic construct EIVLTQSPSSLSASLGDRVTISCRASQDISNFLNWYQQKPDGTVKLLIYYTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQNSKHPWTFGGGTKLEIK 10 AA PAT source 1..10 mol_type protein note 41A07A03/7TI-046 CDR-H1 organism synthetic construct GFTFSSYAMS 17 AA PAT source 1..17 mol_type protein note 41A07A03/7TI-046 CDR-H2 organism synthetic construct FISSGSSTIFYADTVEG 12 AA PAT source 1..12 mol_type protein note 41A07A03/7TI-046 CDR-H3 organism synthetic construct ARRSLDYYAMDY 11 AA PAT source 1..11 mol_type protein note 41A07A03/7TI-046 CDR-L1 organism synthetic construct RASQDISNFLN 7 AA PAT source 1..7 mol_type protein note 41A07A03/7TI-046 CDR-L2 organism synthetic construct YTSRLHS 9 AA PAT source 1..9 mol_type protein note 41A07A03/7TI-046 CDR-L3 organism synthetic construct QQNSKHPWT 117 AA PAT source 1..117 mol_type protein note Full-length sequence of VH of 1KP-3A6-3C6/1KP1-3A6-3C6-hz organism synthetic construct EVQLQQSGPELVKPGASVKISCKASGYTFTHYYINWVKQRPGQGLEWIGWISPGSVNTKYNEKFKGKATLTVDTSSSTAYMQLSSLTSDDSAVYFCATEVYDYGDYWGQGTTVTVSS 107 AA PAT source 1..107 mol_type protein note Full-length sequence of VL of 1KP-3A6-3C6/1KP1-3A6-3C6-hz organism synthetic construct DIVMTQSPKFMSTSVGDRVSVTCKASQNVGTNVAWYQQKPGQSPKALIFSASYRYNGVPDRFTGSGSGTDFTLTITNVQSEDLAEYFCQQYNIYPYTFGGGTKLEIK 10 AA PAT source 1..10 mol_type protein note 1KP-3A6-3C6/1KP1-3A6-3C6-hz CDR-H1 organism synthetic construct GYTFTHYYIN 17 AA PAT source 1..17 mol_type protein note 1KP-3A6-3C6/1KP1-3A6-3C6-hz CDR-H2 organism synthetic construct WISPGSVNTKYNEKFKG 10 AA PAT source 1..10 mol_type protein note 1KP-3A6-3C6/1KP1-3A6-3C6-hz CDR-H3 organism synthetic construct ATEVYDYGDY 11 AA PAT source 1..11 mol_type protein note 1KP-3A6-3C6/1KP1-3A6-3C6-hz CDR-L1 organism synthetic construct KASQNVGTNVA 7 AA PAT source 1..7 mol_type protein note 1KP-3A6-3C6/1KP1-3A6-3C6-hz CDR-L2 organism synthetic construct SASYRYN 9 AA PAT source 1..9 mol_type protein note 1KP-3A6-3C6/1KP1-3A6-3C6-hz CDR-L3 organism synthetic construct QQYNIYPYT 116 AA PAT source 1..116 mol_type protein note Full-length sequence of VH of 1KP1-1E10-F6/1KP1-1E10-F6-hz organism synthetic construct EVQLQQSGPELVKPGASVKISCKASGYTFTDYYMNWVKQSHGKSLEWIGDINPKYGDTTYNLRFQDKATLTVDKSSSTAYMELRSLTSEDFAVYFCARGIRLFDYWGQGTTVTVSS 107 AA PAT source 1..107 mol_type protein note Full-length sequence of VL of 1KP1-1E10-F6/1KP1-1E10-F6-hz organism synthetic construct DIQMTQTTASLSASVGETVTITCRASGNIHNYLAWYQQKQGKSPQILVYNAKTLAEGVPSRFSGSVSGTQYSLKINSLQPEDFGSYYCHHYYTSPFTFGGGTKLEIK 10 AA PAT source 1..10 mol_type protein note 1KP1-1E10-F6/1KP1-1E10-F6-hz CDR-H1 organism synthetic construct GYTFTDYYMN 17 AA PAT source 1..17 mol_type protein note 1KP1-1E10-F6/1KP1-1E10-F6-hz CDR-H2 organism synthetic construct DINPKYGDTTYNLRFQD 9 AA PAT source 1..9 mol_type protein note 1KP1-1E10-F6/1KP1-1E10-F6-hz CDR-H3 organism synthetic construct ARGIRLFDY 11 AA PAT source 1..11 mol_type protein note 1KP1-1E10-F6/1KP1-1E10-F6-hz CDR-L1 organism synthetic construct RASGNIHNYLA 7 AA PAT source 1..7 mol_type protein note 1KP1-1E10-F6/1KP1-1E10-F6-hz CDR-L2 organism synthetic construct NAKTLAE 9 AA PAT source 1..9 mol_type protein note 1KP1-1E10-F6/1KP1-1E10-F6-hz CDR-L3 organism synthetic construct HHYYTSPFT 119 AA PAT source 1..119 mol_type protein note 7TI-208 VH organism synthetic construct QVQLVESGGGLVKPGGSLRLSCAASGFTFSDYGMHWFRQAPGKGLEWVSYISSGSSTIYYADTVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARYRFDAYAMDYWGQGTLVTVSS 107 AA PAT source 1..107 mol_type protein note 7TI-208 VL organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQDISNYLNWYQQKPGGAVKLLIYYKSRLHSGVPSRFSGSGSGTDYTLTISSLQPEDIATYYCQHFRKVPWTFGQGTKVEIK 119 AA PAT source 1..119 mol_type protein note 7TI-202 VH organism synthetic construct QVQLVESGGGLVKPGGSLRLSCAASGFTFSDYGMHWIRQAPGKGLEWVSYISSGSSTIYYADTVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARYRFDAYAMDYWGQGTLVTVSS 107 AA PAT source 1..107 mol_type protein note 7TI-202 VL organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQDISNYLNWYQQKPGGAPKLLIYYKSRLHSGVPSRFSGSGSGTDYTLTISSLQPEDIATYYCQHFRKVPWTFGQGTKVEIK 118 AA PAT source 1..118 mol_type protein note 7TI-172/7TI-174 VH organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYWINWVRQAPGQGLEWMGNIYPALDSTNYNEKFKSRVTMTVDTSTSTAYMELRSLRSDDTAVYYCARLRQFGLFDQWGQGTLVTVSS 106 AA PAT source 1..106 mol_type protein note 7TI-172 VL organism synthetic construct EIVMTQSPATLSLSPGERATLSCSASSGVSYMHWYQQKPGQAPRLLIYDTSKLASGIPARFSGSGSGTDYTLTISSLEPEDFAVYYCQQWSNNPLTFGGGTKVEIK 106 AA PAT source 1..106 mol_type protein note 7TI-174 VL organism synthetic construct EIVMTQSPATLSLSPGERATLSCSASSGVSYMHWYQQKPGQAPRLLIFDTSKLASGIPARFSGSGSGTDYTLTISSMEPEDFAVYYCQQWSNNPLTFGGGTKVEIK 119 AA PAT source 1..119 mol_type protein note 7TI-225/7TI-224 VH organism synthetic construct EVQLVESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVAFISSGSSTIFYADTVEGRFTISRDNAKNSLYLQMNSLRDEDTAVYYCARRSLDYYAMDYWGQGTLVTVSS 107 AA PAT source 1..107 mol_type protein note 7TI-225 VL organism synthetic construct DIQLTQSPSSLSASVGDRVTITCRASQDISNFLNWYQQKPGGAPKLLLYYTSRLHSGVPSRFSGSGSGTDYTLTISSLQPEDFATYYCQQNSKHPWTFGQGTKVEIK 107 AA PAT source 1..107 mol_type protein note 7TI-224 VL organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQDISNFLNWYQQKPGKAPKLLLYYTSRLHSGVPSRFSGSGSGTDYTLTISSLQPEDFATYYCQQNSKHPWTFGQGTKVEIK 116 AA PAT source 1..116 mol_type protein note 7PD-079 VH organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTDYYMNWVRQAPGQGLEWMGDINPKYGDTTYNLRFQDRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGIRLFDYWGQGTLVTVSS 107 AA PAT source 1..107 mol_type protein note 7PD-079/7PD-082 VL organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASGNIHNYLAWYQQKPGKAPKLLLYNAKTLAEGVPSRFSGSGSGTDYTLTISSLQPEDFATYYCHHYYTSPFTFGGGTKVEIK 116 AA PAT source 1..116 mol_type protein note 7PD-082 VH organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTDYYMNWVRQAPGQGLEWMGDINPKYGDTTYNLRFQDRVTMTVDTSTSTVYMELSSLRSEDTAVYYCARGIRLFDYWGQGTLVTVSS 117 AA PAT source 1..117 mol_type protein note 7PD-111 VH organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTHYYINWVRQAPGQGLEWMGWISPGSVNTKYNEKFKGRVTMTVDTSISTAYMELSRLRSDDTAVYFCATEVYDYGDYWGQGTLVTVSS 107 AA PAT source 1..107 mol_type protein note 7PD-111 VL organism synthetic construct DIQMTQSPSSLSASVGDRVTITCKASQNVGTNVAWYQQKPEKSPKALIFSASYRYNGVPSRFSGSGSGTDFTLTISSLQPEDFATYFCQQYNIYPYTFGQGTKLEIK 117 AA PAT source 1..117 mol_type protein note 7PD-118/7PD-120 VH organism synthetic construct QVQLVQSGAEVVKPGASVKVSCKASGYTFTHYYINWVRQRPGQGLEWIGWISPGSVNTKYNEKFKGRVTLTVDTSISTAYMELSRLRSDDTAVYYCATEVYDYGDYWGQGTLVTVSS 107 AA PAT source 1..107 mol_type protein note 7PD-118/7PD-098 VL organism synthetic construct DIQMTQSPSSLSASVGDRVTITCKASQNVGTNVAWYQQKPEKAPKSLIFSASYRYNGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYNIYPYTFGQGTKLEIK 117 AA PAT source 1..117 mol_type protein note 7PD-115 VH organism synthetic construct QVQLVQSGAEVVKPGASVKVSCKASGYTFTHYYINWVRQAPGQGLEWIGWISPGSVNTKYNEKFKGRVTMTVDTSISTAYMELSRLRSDDTAVYYCATEVYDYGDYWGQGTLVTVSS 107 AA PAT source 1..107 mol_type protein note 7PD-115/7PD-120 VL organism synthetic construct DIQMTQSPSSLSASVGDRVTITCKASQNVGTNVAWYQQKPEKSPKALIFSASYRYNGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYNIYPYTFGQGTKLEIK 117 AA PAT source 1..117 mol_type protein note 7PD-098/7PD-099 VH organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTHYYINWVRQAPGQGLEWMGWISPGSVNTKYNEKFKGRVTMTRDTSISTAYMELSRLRSDDTAVYYCATEVYDYGDYWGQGTLVTVSS 107 AA PAT source 1..107 mol_type protein note 7PD-099/7PD-104 VL organism synthetic construct DIQMTQSPSSLSASVGDRVTITCKASQNVGTNVAWYQQKPEKAPKALIFSASYRYNGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYNIYPYTFGQGTKLEIK 117 AA PAT source 1..117 mol_type protein note 7PD-104 VH organism synthetic construct QVQLVQSGAEVKKPGASVKVSCKASGYTFTHYYINWVRQAPGQGLEWMGWISPGSVNTKYNEKFKGRVTMTVDTSISTAYMELSRLRSDDTAVYYCATEVYDYGDYWGQGTLVTVSS 114 AA PAT source 1..114 mol_type protein note IL-15 organism synthetic construct NWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVENLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS 77 AA PAT source 1..77 mol_type protein note IL-15Ra_1 organism synthetic construct PPPMSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKCIRDPALVHQRPAPPSTV 9 AA PAT source 1..9 mol_type protein note Hinge region 1 organism synthetic construct EPKSCPPCP 4 AA PAT source 1..4 mol_type protein note Hinge region 2 organism synthetic construct PPCP 10 AA PAT source 1..10 mol_type protein note Linker 1 organism synthetic construct GGGGSGGGGS 15 AA PAT source 1..15 mol_type protein note Linker 2 organism synthetic construct GGGGSGGGGSGGGGS 20 AA PAT source 1..20 mol_type protein note Linker 3 organism synthetic construct SGGSGGGGSGGGSGGGGSLQ 330 AA PAT source 1..330 mol_type protein note IgG1-CH organism synthetic construct ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 217 AA PAT source 1..217 mol_type protein note IgG1-Knob organism synthetic construct APELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 217 AA PAT source 1..217 mol_type protein note IgG1-Hole organism synthetic construct APELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 107 AA PAT source 1..107 mol_type protein note IgG1-CL organism synthetic construct RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC 98 AA PAT source 1..98 mol_type protein note IgG1-CH1 organism synthetic construct ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKV 80 AA PAT source 1..80 mol_type protein note IL-15Ra_2 organism synthetic construct ITCPPPMSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKCIRDPALVHQRPAPPSTV 20 AA PAT source 1..20 mol_type protein note signal peptide organism synthetic construct METDTLLLWVLLLWVPGSTG 735 DNA PAT source 1..735 mol_type other DNA note huTIGIT target gene organism synthetic construct atgcgctggtgtctcctcctgatctgggcccaggggctgaggcaggctcccctcgcctcaggaatgatgacaggcacaatagaaacaacggggaacatttctgcagagaaaggtggctctatcatcttacaatgtcacctctcctccaccacggcacaagtgacccaggtcaactgggagcagcaggaccagcttctggccatttgtaatgctgacttggggtggcacatctccccatccttcaaggatcgagtggccccaggtcccggcctgggcctcaccctccagtcgctgaccgtgaacgatacaggggagtacttctgcatctatcacacctaccctgatgggacgtacactgggagaatcttcctggaggtcctagaaagctcagtggctgagcacggtgccaggttccagattccattgcttggagccatggccgcgacgctggtggtcatctgcacagcagtcatcgtggtggtcgcgttgactagaaagaagaaagccctcagaatccattctgtggaaggtgacctcaggagaaaatcagctggacaggaggaatggagccccagtgctccctcacccccaggaagctgtgtccaggcagaagctgcacctgctgggctctgtggagagcagcggggagaggactgtgccgagctgcatgactacttcaatgtcctgagttacagaagcctgggtaactgcagcttcttcacagagactggttag 939 DNA PAT source 1..939 mol_type other DNA note cynoTIGIT target gene organism synthetic construct atggcctttcttgtggctccccctatgcagtttgtgtatttgttgaaaactttatgtgtttttaacatggtttttgccaaacctggtttttctgagaccgtcttttctcataggctcagttttaccgtcctatctgcagtcggctactttcggtggcagaagaggccacatctgcttcctgtaagccctctgggcagaagcatgcggtggtgtctcttcctgatctgggcccaggggctgaggcaggctcccctcgcctcaggaatgatgacaggcacaatagaaacaacggggaacatttctgcaaagaaaggtggctctgttatcttacaatgtcacctctcctccaccatggcacaagtgacccaggtcaactgggagcagcatgaccattcgcttctggccattcgtaatgctgagttggggtggcacatctacccagccttcaaggatcgagtggccccgggtcctggcctgggcctcaccctccagtcgctgaccatgaatgatacaggggagtacttctgcacctatcacacctaccctgatgggacttacagagggagaatcttcctggaggtcctagaaagctcagtggctgagcacagtgccaggttccagattccattgcttggagccatggccatgatgctggtggtcatctgcatagcagtcatcgtggtggtcgtgttggctagaaagaagaaatccctcagaatccattctgtggaaagtggcctccagagaaaatcaactggacaggaagaacagattcccagtgctccctcacccccaggaagctgtgtccaggcagaagctgcacctgctgggctctgtggagagcagcagggagatgactgtgccgagctgcatgactacttcaatgtcctgagttacagaagcctggggagctgcagcttcttcacagagactgggtag 321 DNA PAT source 1..321 mol_type other DNA note Human IGKC nucleotide organism synthetic construct cgcaccgtagctgccccgtccgtgtttatattcccaccaagcgatgagcaactcaagtccggtactgcttcagtcgtttgtcttcttaataatttctatcctagagaagctaaagtacaatggaaagtagataacgcccttcaatccgggaattcacaggagagcgttacggagcaggatagtaaggacagcacctacagcctgagtagcacgctcactctgtcaaaggcagattatgagaaacacaaagtctatgcgtgtgaggtgactcatcagggactcagcagcccagtaacaaagagttttaataggggtgagtgc