1264-WO-PCT EP EP21197120.5 2021-09-16 H. Lundbeck A/S COMPOSITIONS AND METHODS FOR TREATING SYNUCLEINOPATHIES 43 10 AA PAT REGION 1..10 note GM37 CDR 1 Heavy Chain source 1..10 mol_type protein organism synthetic construct GFTFSSYAMT 17 AA PAT REGION 1..17 note GM37 CDR2 Heavy Chain source 1..17 mol_type protein organism synthetic construct AIRSNGDRTDYADSVKG 9 AA PAT REGION 1..9 note GM37 CDR3 Heavy Chain source 1..9 mol_type protein organism synthetic construct AKNWAPFDS 11 AA PAT REGION 1..11 note GM37 CDR1 Light Chain source 1..11 mol_type protein organism synthetic construct ASQSVSSSYLA 7 AA PAT REGION 1..7 note GM37 CDR 2 Light Chain source 1..7 mol_type protein organism synthetic construct GASSRAT 9 AA PAT REGION 1..9 note GM37 CDR 3 Light Chain source 1..9 mol_type protein organism synthetic construct QQYGSSPWT 116 AA PAT REGION 1..116 note GM37 CDR Heavy Chain source 1..116 mol_type protein organism synthetic construct EVQLLESGGGLVQTGGSLRLSCAASGFTFSSYAMTWVRQAPGKGLEWVSAIRSNGDRTDYADSVKGRFTISRDNSQNTLYLQMNSLRAEDTAVYYCAKNWAPFDSWGQGTLVTVSS 108 AA PAT REGION 1..108 note GM 37 Light Chain source 1..108 mol_type protein organism synthetic construct EIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPWTFGQGTKVEIK 6 AA PAT REGION 1..6 note Epitope 112-117 source 1..6 mol_type protein organism synthetic construct ILEDMP 140 AA PAT REGION 1..140 note Alpha-synuclein source 1..140 mol_type protein organism synthetic construct MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA 165 AA PAT REGION 1..165 note A-Syn-AAKK-BAP source 1..165 mol_type protein organism synthetic construct MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGNIAAATGLVKKDQLAKQNEEGFLQEGMVNNTDIPVDPENEAYEMPPEEEYQDYEPEAGSAGGSGGLNDIFEAQKIEWHE 165 AA PAT REGION 1..165 note A-Syn-BAAK-BAP source 1..165 mol_type protein organism synthetic construct MDVFMKGLSMAKEGVVAAAEKTKQGVTEAAEKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLAKQNEEGFLQEGMVNNTDIPVDPENEAYEMPPEEEYQDYEPEAGSAGGSGGLNDIFEAQKIEWHE 162 AA PAT REGION 1..162 note A-Syn-BBAA-BAP source 1..162 mol_type protein organism synthetic construct MDVFMKGLSMAKEGVVAAAEKTKQGVTEAAEKTKEGVLYVGSKTREGVVQGVASVAEKTKEQASHLGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEAGSAGGSGGLNDIFEAQKIEWHE 165 AA PAT REGION 1..165 note A-Syn-BBKK-BAP source 1..165 mol_type protein organism synthetic construct MDVFMKGLSMAKEGVVAAAEKTKQGVTEAAEKTKEGVLYVGSKTREGVVQGVASVAEKTKEQASHLGGAVVTGVTAVAQKTVEGAGNIAAATGLVKKDQLAKQNEEGFLQEGMVNNTDIPVDPENEAYEMPPEEEYQDYEPEAGSAGGSGGLNDIFEAQKIEWHE 141 AA PAT REGION 1..141 note A-Syn-120-140_Del-BAP source 1..141 mol_type protein organism synthetic construct MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDGSAGGSGGLNDIFEAQKIEWHE 131 AA PAT REGION 1..131 note alpha-synuclein amino acids 1-119 source 1..131 mol_type protein organism synthetic construct MAHHHHHHIEGRMDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVD 107 AA PAT REGION 1..107 note kappa (LC constant region) source 1..107 mol_type protein organism synthetic construct RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC 329 AA PAT REGION 1..329 note IgG1 (HC Constant region) source 1..329 mol_type protein organism synthetic construct ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPG 4 AA PAT REGION 1..4 note GM285 epitope 112-115 source 1..4 mol_type protein organism synthetic construct ILED 13 AA PAT REGION 1..13 note GM285 CDR1 Heavy Chain source 1..13 mol_type protein organism synthetic construct AASGFTFSRFTMT 17 AA PAT REGION 1..17 note GM285 CDR2 Heavy Chain source 1..17 mol_type protein organism synthetic construct AISGSGGGTSYADSVKG 9 AA PAT REGION 1..9 note GM285 CDR3 Heavy Chain source 1..9 mol_type protein organism synthetic construct AKNWAPFDY 12 AA PAT REGION 1..12 note GM285 CDR1 Light Chain source 1..12 mol_type protein organism synthetic construct RASQSVSRSYLA 7 AA PAT REGION 1..7 note GM285 CDR2 Light Chain source 1..7 mol_type protein organism synthetic construct GASSRAT 9 AA PAT REGION 1..9 note GM285 CDR3 Light Chain source 1..9 mol_type protein organism synthetic construct QQYGSSPWT 116 AA PAT REGION 1..116 note GM285 VH source 1..116 mol_type protein organism synthetic construct EVQLLESGGGLVQPGGSLRLSCAASGFTFSRFTMTWVRQAPGKGLEWVSAISGSGGGTSYADSVKGRLTVSRDNSKNTLYLQMNSLRAEDTAVYYCAKNWAPFDYWGQGTLVTVSS 108 AA PAT REGION 1..108 note GM285 VL source 1..108 mol_type protein organism synthetic construct EIVLTQSPGTLSLSPGERATLSCRASQSVSRSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTVSRLEPEDFAVYYCQQYGSSPWTFGQGTKVEIK 329 AA PAT REGION 1..329 note GM285 IgG1 constant region source 1..329 mol_type protein organism synthetic construct ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPG 106 AA PAT REGION 1..106 note GM285 Kappa chain source 1..106 mol_type protein organism synthetic construct TVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC 116 AA PAT REGION 1..116 note GM37 Variant 1 heavy chain source 1..116 mol_type protein organism synthetic construct EVQLLESGGGLVQTGGSLRLSCAASGFTFSSYAMTWVRQAPGKGLEWVSAIRSSGDRTDYADSVKGRFTISRDNSQNTLYLQMNSLRAEDTAVYYCAKNWAPFDSWGQGTLVTVSS 116 AA PAT REGION 1..116 note GM 37 variant 2 heavy chain source 1..116 mol_type protein organism synthetic construct EVQLLESGGGLVQTGGSLRLSCAASGFTFSSYAMTWVRQAPGKGLEWVSAIRSQGDRTDYADSVKGRFTISRDNSQNTLYLQMNSLRAEDTAVYYCAKNWAPFDSWGQGTLVTVSS 116 AA PAT REGION 1..116 note GM 37 variant 3 heavy chain source 1..116 mol_type protein organism synthetic construct EVQLLESGGGLVQTGGSLRLSCAASGFTFSSYAMTWVRQAPGKGLEWVSAIRSHGDRTDYADSVKGRFTISRDNSQNTLYLQMNSLRAEDTAVYYCAKNWAPFDSWGQGTLVTVSS 17 AA PAT REGION 1..17 note GM37 variant 1 heavy chain CDR 2 source 1..17 mol_type protein organism synthetic construct AIRSSGDRTDYADSVKG 17 AA PAT REGION 1..17 note GM37 variant 2 CDR 2 heavy chain source 1..17 mol_type protein organism synthetic construct AIRSQGDRTDYADSVKG 17 AA PAT REGION 1..17 note GM37 variant 3 CDR 2 heavy chain source 1..17 mol_type protein organism synthetic construct AIRSHGDRTDYADSVKG 5 AA PAT REGION 1..5 note 9E4 binding epitope source 1..5 mol_type protein organism synthetic construct NEAYE 134 AA PAT REGION 1..134 note HUMAN Beta-synuclein source 1..134 mol_type protein organism synthetic construct MDVFMKGLSMAKEGVVAAAEKTKQGVTEAAEKTKEGVLYVGSKTREGVVQGVASVAEKTKEQASHLGGAVFSGAGNIAAATGLVKREEFPTDLKPEEVAQEAAEEPLIEPLMEPEGESYEDPPQEEYQEYEPEA 127 AA PAT REGION 1..127 note HUMAN Gamma-synuclein source 1..127 mol_type protein organism synthetic construct MDVFKKGFSIAKEGVVGAVEKTKQGVTEAAEKTKEGVMYVGAKTKENVVQSVTSVAEKTKEQANAVSEAVVSSVNTVATKTVEEAENIAVTSGVVRKEDLRPSAPQQEGEASKEKEEVAEEAQSGGD 140 AA PAT REGION 1..140 note alpha-synuclein ortholog for Cynomolgus monkey source 1..140 mol_type protein organism synthetic construct MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFIKKDQLGKNEEGAPQEGILQDMPVDPDNEAYEMPSEEGYQDYEPEA 140 AA PAT REGION 1..140 note alpha-synuclein ortholog for Rat source 1..140 mol_type protein organism synthetic construct MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVTTVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGNIAAATGFVKKDQMGKGEEGYPQEGILEDMPVDPSSEAYEMPSEEGYQDYEPEA 140 AA PAT REGION 1..140 note alpha-synuclein ortholog for Mouse source 1..140 mol_type protein organism synthetic construct MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVTTVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGNIAAATGFVKKDQMGKGEEGYPQEGILEDMPVDPGSEAYEMPSEEGYQDYEPEA 446 AA PAT REGION 1..446 note 9E4 HC source 1..446 mol_type protein organism synthetic construct EVQLVESGGGLVQPGGSLRLSCAASGFTFSNYGMSWVRQAPGKGLEWVASISSGGGSTYYPDNVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARGGAGIDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 220 AA PAT REGION 1..220 note 9E4 LC source 1..220 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCKSIQTLLYSSNQKNYLAWFQQKPGKAPKLLIYWASIRKSGVPSRFSGSGSGTDFTLTISSLQPEDLATYYCQQYYSYPLTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC