WO PCT/US22/74837 2022-08-11 122864-5066-WO US US 63/232,141 2021-08-11 Sana Biotechnology, Inc. INDUCIBLE SYSTEMS FOR ALTERING GENE EXPRESSION IN HYPOIMMUNOGENIC CELLS 130 000 000 000 000 000 21 AA PAT REGION 1..21 note Synthetic peptide source 1..21 mol_type protein organism synthetic construct MALPVTALLLPLALLLHAARP 20 AA PAT REGION 1..20 note Synthetic peptide source 1..20 mol_type protein organism synthetic construct METDTLLLWVLLLWVPGSTG 22 AA PAT REGION 1..22 note Synthetic peptide source 1..22 mol_type protein organism synthetic construct MLLLVTSLLLCELPHPAFLLIP 45 AA PAT REGION 1..45 note Synthetic hinge domain source 1..45 mol_type protein organism synthetic construct TTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACD 39 AA PAT REGION 1..39 note Synthetic hinge domain source 1..39 mol_type protein organism synthetic construct IEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKP 12 AA PAT REGION 1..12 note Synthetic hinge domain source 1..12 mol_type protein organism synthetic construct ESKYGPPCPPCP 12 AA PAT REGION 1..12 note Synthetic hinge domain source 1..12 mol_type protein organism synthetic construct ESKYGPPCPSCP 229 AA PAT REGION 1..229 note Synthetic hinge domain source 1..229 mol_type protein organism synthetic construct ESKYGPPCPPCPAPEFLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSLGK 24 AA PAT REGION 1..24 note Synthetic transmembrane domain source 1..24 mol_type protein organism synthetic construct IYIWAPLAGTCGVLLLSLVITLYC 27 AA PAT REGION 1..27 note Synthetic transmembrane domain source 1..27 mol_type protein organism synthetic construct FWVLVVVGGVLACYSLLVTVAFIIFWV 42 AA PAT REGION 1..42 note Synthetic costimulatory domain source 1..42 mol_type protein organism synthetic construct KRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCEL 41 AA PAT REGION 1..41 note Synthetic costimulatory domain source 1..41 mol_type protein organism synthetic construct RSKRSRLLHSDYMNMTPRRPGPTRKHYQPYAPPRDFAAYRS 112 AA PAT REGION 1..112 note Synthetic signaling domain source 1..112 mol_type protein organism synthetic construct RVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 245 AA PAT REGION 1..245 note Synthetic amino acid source 1..245 mol_type protein organism synthetic construct DIQMTQTTSSLSASLGDRVTISCRASQDISKYLNWYQQKPDGTVKLLIYHTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQGNTLPYTFGGGTKLEITGSTSGSGKPGSGEGSTKGEVKLQESGPGLVAPSQSLSVTCTVSGVSLPDYGVSWIRQPPRKGLEWLGVIWGSETTYYNSALKSRLTIIKDNSKSQVFLKMNSLQTDDTAIYYCAKHYYYGGSYAMDYWGQGTSVTVSS 107 AA PAT REGION 1..107 note Synthetic amino acid source 1..107 mol_type protein organism synthetic construct DIQMTQTTSSLSASLGDRVTISCRASQDISKYLNWYQQKPDGTVKLLIYHTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQGNTLPYTFGGGTKLEIT 6 AA PAT REGION 1..6 note Synthetic amino acid source 1..6 mol_type protein organism synthetic construct QDISKY 000 9 AA PAT REGION 1..9 note Synthetic amino acid source 1..9 mol_type protein organism synthetic construct QQGNTLPYT 18 AA PAT REGION 1..18 note Synthetic amino acid linker source 1..18 mol_type protein organism synthetic construct GSTSGSGKPGSGEGSTKG 120 AA PAT REGION 1..120 note Synthetic amino acid source 1..120 mol_type protein organism synthetic construct EVKLQESGPGLVAPSQSLSVTCTVSGVSLPDYGVSWIRQPPRKGLEWLGVIWGSETTYYNSALKSRLTIIKDNSKSQVFLKMNSLQTDDTAIYYCAKHYYYGGSYAMDYWGQGTSVTVSS 8 AA PAT REGION 1..8 note Synthetic amino acid source 1..8 mol_type protein organism synthetic construct GVSLPDYG 7 AA PAT REGION 1..7 note Synthetic amino acid source 1..7 mol_type protein organism synthetic construct IWGSETT 14 AA PAT REGION 1..14 note Synthetic amino acid source 1..14 mol_type protein organism synthetic construct AKHYYYGGSYAMDY 242 AA PAT REGION 1..242 note Synthetic amino acid source 1..242 mol_type protein organism synthetic construct DIQMTQTTSSLSASLGDRVTISCRASQDISKYLNWYQQKPDGTVKLLIYHTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQGNTLPYTFGGGTKLEITGGGGSGGGGSGGGGSEVKLQESGPGLVAPSQSLSVTCTVSGVSLPDYGVSWIRQPPRKGLEWLGVIWGSETTYYNSALKSRLTIIKDNSKSQVFLKMNSLQTDDTAIYYCAKHYYYGGSYAMDYWGQGTSVTVSS 15 AA PAT REGION 1..15 note Synthetic amino acid linker source 1..15 mol_type protein organism synthetic construct GGGGSGGGGSGGGGS 1458 DNA PAT misc_feature 1..1458 note Synthetic nucleotide sequence source 1..1458 mol_type other DNA organism synthetic construct atggccttaccagtgaccgccttgctcctgccgctggccttgctgctccacgccgccaggccggacatccagatgacacagactacatcctccctgtctgcctctctgggagacagagtcaccatcagttgcagggcaagtcaggacattagtaaatatttaaattggtatcagcagaaaccagatggaactgttaaactcctgatctaccatacatcaagattacactcaggagtcccatcaaggttcagtggcagtgggtctggaacagattattctctcaccattagcaacctggagcaagaagatattgccacttacttttgccaacagggtaatacgcttccgtacacgttcggaggggggaccaagctggagatcacaggtggcggtggctcgggcggtggtgggtcgggtggcggcggatctgaggtgaaactgcaggagtcaggacctggcctggtggcgccctcacagagcctgtccgtcacatgcactgtctcaggggtctcattacccgactatggtgtaagctggattcgccagcctccacgaaagggtctggagtggctgggagtaatatggggtagtgaaaccacatactataattcagctctcaaatccagactgaccatcatcaaggacaactccaagagccaagttttcttaaaaatgaacagtctgcaaactgatgacacagccatttactactgtgccaaacattattactacggtggtagctatgctatggactactggggccaaggaacctcagtcaccgtctcctcaaccacgacgccagcgccgcgaccaccaacaccggcgcccaccatcgcgtcgcagcccctgtccctgcgcccagaggcgtgccggccagcggcggggggcgcagtgcacacgagggggctggacttcgcctgtgatatctacatctgggcgcccttggccgggacttgtggggtccttctcctgtcactggttatcaccctttactgcaaacggggcagaaagaaactcctgtatatattcaaacaaccatttatgagaccagtacaaactactcaagaggaagatggctgtagctgccgatttccagaagaagaagaaggaggatgtgaactgagagtgaagttcagcaggagcgcagacgcccccgcgtacaagcagggccagaaccagctctataacgagctcaatctaggacgaagagaggagtacgatgttttggacaagagacgtggccgggaccctgagatggggggaaagccgagaaggaagaaccctcaggaaggcctgtacaatgaactgcagaaagataagatggcggaggcctacagtgagattgggatgaaaggcgagcgccggaggggcaaggggcacgatggcctttaccagggtctcagtacagccaccaaggacacctacgacgcccttcacatgcaggccctgccccctcgc 486 AA PAT REGION 1..486 note Synthetic amino acid source 1..486 mol_type protein organism synthetic construct MALPVTALLLPLALLLHAARPDIQMTQTTSSLSASLGDRVTISCRASQDISKYLNWYQQKPDGTVKLLIYHTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQGNTLPYTFGGGTKLEITGGGGSGGGGSGGGGSEVKLQESGPGLVAPSQSLSVTCTVSGVSLPDYGVSWIRQPPRKGLEWLGVIWGSETTYYNSALKSRLTIIKDNSKSQVFLKMNSLQTDDTAIYYCAKHYYYGGSYAMDYWGQGTSVTVSSTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYKQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 1383 DNA PAT misc_feature 1..1383 note Synthetic nucleotide sequence source 1..1383 mol_type other DNA organism synthetic construct atgctgctgctggtgaccagcctgctgctgtgcgagctgccccaccccgcctttctgctgatccccgacatccagatgacccagaccacctccagcctgagcgccagcctgggcgaccgggtgaccatcagctgccgggccagccaggacatcagcaagtacctgaactggtatcagcagaagcccgacggcaccgtcaagctgctgatctaccacaccagccggctgcacagcggcgtgcccagccggtttagcggcagcggctccggcaccgactacagcctgaccatctccaacctggaacaggaagatatcgccacctacttttgccagcagggcaacacactgccctacacctttggcggcggaacaaagctggaaatcaccggcagcacctccggcagcggcaagcctggcagcggcgagggcagcaccaagggcgaggtgaagctgcaggaaagcggccctggcctggtggcccccagccagagcctgagcgtgacctgcaccgtgagcggcgtgagcctgcccgactacggcgtgagctggatccggcagccccccaggaagggcctggaatggctgggcgtgatctggggcagcgagaccacctactacaacagcgccctgaagagccggctgaccatcatcaaggacaacagcaagagccaggtgttcctgaagatgaacagcctgcagaccgacgacaccgccatctactactgcgccaagcactactactacggcggcagctacgccatggactactggggccagggcaccagcgtgaccgtgagcagcgaatctaagtacggaccgccctgccccccttgccctatgttctgggtgctggtggtggtcggaggcgtgctggcctgctacagcctgctggtcaccgtggccttcatcatcttttgggtgaaacggggcagaaagaaactcctgtatatattcaaacaaccatttatgagaccagtacaaactactcaagaggaagatggctgtagctgccgatttccagaagaagaagaaggaggatgtgaactgcgggtgaagttcagcagaagcgccgacgcccctgcctaccagcagggccagaatcagctgtacaacgagctgaacctgggcagaagggaagagtacgacgtcctggataagcggagaggccgggaccctgagatgggcggcaagcctcggcggaagaacccccaggaaggcctgtataacgaactgcagaaagacaagatggccgaggcctacagcgagatcggcatgaagggcgagcggaggcggggcaagggccacgacggcctgtatcagggcctgtccaccgccaccaaggatacctacgacgccctgcacatgcaggccctgcccccaagg 461 AA PAT REGION 1..461 note Synthetic amino acid source 1..461 mol_type protein organism synthetic construct MLLLVTSLLLCELPHPAFLLIPDIQMTQTTSSLSASLGDRVTISCRASQDISKYLNWYQQKPDGTVKLLIYHTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQGNTLPYTFGGGTKLEITGSTSGSGKPGSGEGSTKGEVKLQESGPGLVAPSQSLSVTCTVSGVSLPDYGVSWIRQPPRKGLEWLGVIWGSETTYYNSALKSRLTIIKDNSKSQVFLKMNSLQTDDTAIYYCAKHYYYGGSYAMDYWGQGTSVTVSSESKYGPPCPPCPMFWVLVVVGGVLACYSLLVTVAFIIFWVKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 1467 DNA PAT misc_feature 1..1467 note Synthetic nucleotide sequence source 1..1467 mol_type other DNA organism synthetic construct atgcttctcctggtgacaagccttctgctctgtgagttaccacacccagcattcctcctgatcccagacatccagatgacacagactacatcctccctgtctgcctctctgggagacagagtcaccatcagttgcagggcaagtcaggacattagtaaatatttaaattggtatcagcagaaaccagatggaactgttaaactcctgatctaccatacatcaagattacactcaggagtcccatcaaggttcagtggcagtgggtctggaacagattattctctcaccattagcaacctggagcaagaagatattgccacttacttttgccaacagggtaatacgcttccgtacacgttcggaggggggactaagttggaaataacaggctccacctctggatccggcaagcccggatctggcgagggatccaccaagggcgaggtgaaactgcaggagtcaggacctggcctggtggcgccctcacagagcctgtccgtcacatgcactgtctcaggggtctcattacccgactatggtgtaagctggattcgccagcctccacgaaagggtctggagtggctgggagtaatatggggtagtgaaaccacatactataattcagctctcaaatccagactgaccatcatcaaggacaactccaagagccaagttttcttaaaaatgaacagtctgcaaactgatgacacagccatttactactgtgccaaacattattactacggtggtagctatgctatggactactggggtcaaggaacctcagtcaccgtctcctcagcggccgcaattgaagttatgtatcctcctccttacctagacaatgagaagagcaatggaaccattatccatgtgaaagggaaacacctttgtccaagtcccctatttcccggaccttctaagcccttttgggtgctggtggtggttgggggagtcctggcttgctatagcttgctagtaacagtggcctttattattttctgggtgaggagtaagaggagcaggctcctgcacagtgactacatgaacatgactccccgccgccccgggcccacccgcaagcattaccagccctatgccccaccacgcgacttcgcagcctatcgctccagagtgaagttcagcaggagcgcagacgcccccgcgtaccagcagggccagaaccagctctataacgagctcaatctaggacgaagagaggagtacgatgttttggacaagagacgtggccgggaccctgagatggggggaaagccgagaaggaagaaccctcaggaaggcctgtacaatgaactgcagaaagataagatggcggaggcctacagtgagattgggatgaaaggcgagcgccggaggggcaaggggcacgatggcctttaccagggtctcagtacagccaccaaggacacctacgacgcccttcacatgcaggccctgccccctcgc 489 AA PAT REGION 1..489 note Synthetic amino acid source 1..489 mol_type protein organism synthetic construct MLLLVTSLLLCELPHPAFLLIPDIQMTQTTSSLSASLGDRVTISCRASQDISKYLNWYQQKPDGTVKLLIYHTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQGNTLPYTFGGGTKLEITGSTSGSGKPGSGEGSTKGEVKLQESGPGLVAPSQSLSVTCTVSGVSLPDYGVSWIRQPPRKGLEWLGVIWGSETTYYNSALKSRLTIIKDNSKSQVFLKMNSLQTDDTAIYYCAKHYYYGGSYAMDYWGQGTSVTVSSAAAIEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKPFWVLVVVGGVLACYSLLVTVAFIIFWVRSKRSRLLHSDYMNMTPRRPGPTRKHYQPYAPPRDFAAYRSRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 246 AA PAT REGION 1..246 note Synthetic amino acid source 1..246 mol_type protein organism synthetic construct DIVLTQSPAILSASPGEKVTMTCRASSSVNYMDWYQKKPGSSPKPWIYATSNLASGVPARFSGSGSGTSYSLTISRVEAEDAATYYCQQWSFNPPTFGGGTKLEIKGSTSGSGKPGSGEGSTKGEVQLQQSGAELVKPGASVKMSCKASGYTFTSYNMHWVKQTPGQGLEWIGAIYPGNGDTSYNQKFKGKATLTADKSSSTAYMQLSSLTSEDSADYYCARSNYYGSSYWFFDVWGAGTTVTVSS 106 AA PAT REGION 1..106 note Synthetic amino acid source 1..106 mol_type protein organism synthetic construct DIVLTQSPAILSASPGEKVTMTCRASSSVNYMDWYQKKPGSSPKPWIYATSNLASGVPARFSGSGSGTSYSLTISRVEAEDAATYYCQQWSFNPPTFGGGTKLEIK 10 AA PAT REGION 1..10 note Synthetic amino acid source 1..10 mol_type protein organism synthetic construct RASSSVNYMD 7 AA PAT REGION 1..7 note Synthetic amino acid source 1..7 mol_type protein organism synthetic construct ATSNLAS 9 AA PAT REGION 1..9 note Synthetic amino acid source 1..9 mol_type protein organism synthetic construct QQWSFNPPT 122 AA PAT REGION 1..122 note Synthetic amino acid source 1..122 mol_type protein organism synthetic construct EVQLQQSGAELVKPGASVKMSCKASGYTFTSYNMHWVKQTPGQGLEWIGAIYPGNGDTSYNQKFKGKATLTADKSSSTAYMQLSSLTSEDSADYYCARSNYYGSSYWFFDVWGAGTTVTVSS 5 AA PAT REGION 1..5 note Synthetic amino acid source 1..5 mol_type protein organism synthetic construct SYNMH 17 AA PAT REGION 1..17 note Synthetic amino acid source 1..17 mol_type protein organism synthetic construct AIYPGNGDTSYNQKFKG 246 AA PAT REGION 1..246 note Synthetic amino acid source 1..246 mol_type protein organism synthetic construct QVQLQQSGPGLVKPSQTLSLTCAISGDSVSSNSAAWNWIRQSPSRGLEWLGRTYYRSKWYNDYAVSVKSRITINPDTSKNQFSLQLNSVTPEDTAVYYCAREVTGDLEDAFDIWGQGTMVTVSSGGGGSGGGGSGGGGSDIQMTQSPSSLSASVGDRVTITCRASQTIWSYLNWYQQRPGKAPNLLIYAASSLQSGVPSRFSGRGSGTDFTLTISSLQAEDFATYYCQQSYSIPQTFGQGTKLEIK 124 AA PAT REGION 1..124 note Synthetic amino acid source 1..124 mol_type protein organism synthetic construct QVQLQQSGPGLVKPSQTLSLTCAISGDSVSSNSAAWNWIRQSPSRGLEWLGRTYYRSKWYNDYAVSVKSRITINPDTSKNQFSLQLNSVTPEDTAVYYCAREVTGDLEDAFDIWGQGTMVTVSS 10 AA PAT REGION 1..10 note Synthetic amino acid source 1..10 mol_type protein organism synthetic construct GDSVSSNSAA 9 AA PAT REGION 1..9 note Synthetic amino acid source 1..9 mol_type protein organism synthetic construct TYYRSKWYN 14 AA PAT REGION 1..14 note Synthetic amino acid source 1..14 mol_type protein organism synthetic construct AREVTGDLEDAFDI 107 AA PAT REGION 1..107 note Synthetic amino acid source 1..107 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQTIWSYLNWYQQRPGKAPNLLIYAASSLQSGVPSRFSGRGSGTDFTLTISSLQAEDFATYYCQQSYSIPQTFGQGTKLEIK 6 AA PAT REGION 1..6 note Synthetic amino acid source 1..6 mol_type protein organism synthetic construct QTIWSY 000 9 AA PAT REGION 1..9 note Synthetic amino acid source 1..9 mol_type protein organism synthetic construct QQSYSIPQT 246 AA PAT REGION 1..246 note Synthetic amino acid source 1..246 mol_type protein organism synthetic construct QVQLQQSGPGMVKPSQTLSLTCAISGDSVSSNSVAWNWIRQSPSRGLEWLGRTYYRSTWYNDYAVSMKSRITINPDTNKNQFSLQLNSVTPEDTAVYYCAREVTGDLEDAFDIWGQGTMVTVSSGGGGSGGGGSGGGGSDIQMIQSPSSLSASVGDRVTITCRASQTIWSYLNWYRQRPGEAPNLLIYAASSLQSGVPSRFSGRGSGTDFTLTISSLQAEDFATYYCQQSYSIPQTFGQGTKLEIK 124 AA PAT REGION 1..124 note Synthetic amino acid source 1..124 mol_type protein organism synthetic construct QVQLQQSGPGMVKPSQTLSLTCAISGDSVSSNSVAWNWIRQSPSRGLEWLGRTYYRSTWYNDYAVSMKSRITINPDTNKNQFSLQLNSVTPEDTAVYYCAREVTGDLEDAFDIWGQGTMVTVSS 10 AA PAT REGION 1..10 note Synthetic amino acid source 1..10 mol_type protein organism synthetic construct GDSVSSNSVA 9 AA PAT REGION 1..9 note Synthetic amino acid source 1..9 mol_type protein organism synthetic construct TYYRSTWYN 14 AA PAT REGION 1..14 note Synthetic amino acid source 1..14 mol_type protein organism synthetic construct AREVTGDLEDAFDI 107 AA PAT REGION 1..107 note Synthetic amino acid source 1..107 mol_type protein organism synthetic construct DIQMIQSPSSLSASVGDRVTITCRASQTIWSYLNWYRQRPGEAPNLLIYAASSLQSGVPSRFSGRGSGTDFTLTISSLQAEDFATYYCQQSYSIPQTFGQGTKLEIK 6 AA PAT REGION 1..6 note Synthetic amino acid source 1..6 mol_type protein organism synthetic construct QTIWSY 000 9 AA PAT REGION 1..9 note Synthetic amino acid source 1..9 mol_type protein organism synthetic construct QQSYSIPQT 246 AA PAT REGION 1..246 note Synthetic amino acid source 1..246 mol_type protein organism synthetic construct DIVLTQSPASLAMSLGKRATISCRASESVSVIGAHLIHWYQQKPGQPPKLLIYLASNLETGVPARFSGSGSGTDFTLTIDPVEEDDVAIYSCLQSRIFPRTFGGGTKLEIKGSTSGSGKPGSGEGSTKGQIQLVQSGPELKKPGETVKISCKASGYTFTDYSINWVKRAPGKGLKWMGWINTETREPAYAYDFRGRFAFSLETSASTAYLQINNLKYEDTATYFCALDYSYAMDYWGQGTSVTVSS 111 AA PAT REGION 1..111 note Synthetic amino acid source 1..111 mol_type protein organism synthetic construct DIVLTQSPASLAMSLGKRATISCRASESVSVIGAHLIHWYQQKPGQPPKLLIYLASNLETGVPARFSGSGSGTDFTLTIDPVEEDDVAIYSCLQSRIFPRTFGGGTKLEIK 15 AA PAT REGION 1..15 note Synthetic amino acid source 1..15 mol_type protein organism synthetic construct RASESVSVIGAHLIH 7 AA PAT REGION 1..7 note Synthetic amino acid source 1..7 mol_type protein organism synthetic construct LASNLET 9 AA PAT REGION 1..9 note Synthetic amino acid source 1..9 mol_type protein organism synthetic construct LQSRIFPRT 117 AA PAT REGION 1..117 note Synthetic amino acid source 1..117 mol_type protein organism synthetic construct QIQLVQSGPELKKPGETVKISCKASGYTFTDYSINWVKRAPGKGLKWMGWINTETREPAYAYDFRGRFAFSLETSASTAYLQINNLKYEDTATYFCALDYSYAMDYWGQGTSVTVSS 5 AA PAT REGION 1..5 note Synthetic amino acid source 1..5 mol_type protein organism synthetic construct DYSIN 17 AA PAT REGION 1..17 note Synthetic amino acid source 1..17 mol_type protein organism synthetic construct WINTETREPAYAYDFRG 8 AA PAT REGION 1..8 note Synthetic amino acid source 1..8 mol_type protein organism synthetic construct DYSYAMDY 246 AA PAT REGION 1..246 note Synthetic amino acid source 1..246 mol_type protein organism synthetic construct DIVLTQSPPSLAMSLGKRATISCRASESVTILGSHLIYWYQQKPGQPPTLLIQLASNVQTGVPARFSGSGSRTDFTLTIDPVEEDDVAVYYCLQSRTIPRTFGGGTKLEIKGSTSGSGKPGSGEGSTKGQIQLVQSGPELKKPGETVKISCKASGYTFRHYSMNWVKQAPGKGLKWMGRINTESGVPIYADDFKGRFAFSVETSASTAYLVINNLKDEDTASYFCSNDYLYSLDFWGQGTALTVSS 111 AA PAT REGION 1..111 note Synthetic amino acid source 1..111 mol_type protein organism synthetic construct DIVLTQSPPSLAMSLGKRATISCRASESVTILGSHLIYWYQQKPGQPPTLLIQLASNVQTGVPARFSGSGSRTDFTLTIDPVEEDDVAVYYCLQSRTIPRTFGGGTKLEIK 15 AA PAT REGION 1..15 note Synthetic amino acid source 1..15 mol_type protein organism synthetic construct RASESVTILGSHLIY 7 AA PAT REGION 1..7 note Synthetic amino acid source 1..7 mol_type protein organism synthetic construct LASNVQT 9 AA PAT REGION 1..9 note Synthetic amino acid source 1..9 mol_type protein organism synthetic construct LQSRTIPRT 117 AA PAT REGION 1..117 note Synthetic amino acid source 1..117 mol_type protein organism synthetic construct QIQLVQSGPELKKPGETVKISCKASGYTFRHYSMNWVKQAPGKGLKWMGRINTESGVPIYADDFKGRFAFSVETSASTAYLVINNLKDEDTASYFCSNDYLYSLDFWGQGTALTVSS 5 AA PAT REGION 1..5 note Synthetic amino acid source 1..5 mol_type protein organism synthetic construct HYSMN 17 AA PAT REGION 1..17 note Synthetic amino acid source 1..17 mol_type protein organism synthetic construct RINTESGVPIYADDFKG 8 AA PAT REGION 1..8 note Synthetic amino acid source 1..8 mol_type protein organism synthetic construct DYLYSLDF 121 AA PAT REGION 1..121 note Synthetic amino acid source 1..121 mol_type protein organism synthetic construct EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSSISGSGDYIYYADSVKGRFTISRDISKNTLYLQMNSLRAEDTAVYYCAKEGTGANSSLADYRGQGTLVTVSS 8 AA PAT REGION 1..8 note Synthetic amino acid source 1..8 mol_type protein organism synthetic construct GFTFSSYA 8 AA PAT REGION 1..8 note Synthetic amino acid source 1..8 mol_type protein organism synthetic construct ISGSGDYI 14 AA PAT REGION 1..14 note Synthetic amino acid source 1..14 mol_type protein organism synthetic construct AKEGTGANSSLADY 20 RNA PAT misc_feature 1..20 note synthetic poymer source 1..20 mol_type other RNA organism synthetic construct tctctccatgtgcagtagga 20 RNA PAT misc_feature 1..20 note synthetic poymer source 1..20 mol_type other RNA organism synthetic construct ctggatgtcggaggagtacg 20 RNA PAT misc_feature 1..20 note synthetic poymer source 1..20 mol_type other RNA organism synthetic construct gtctccggaaactcgaggtg 20 RNA PAT misc_feature 1..20 note synthetic poymer source 1..20 mol_type other RNA organism synthetic construct acagtgtagacttgattgac 20 RNA PAT misc_feature 1..20 note synthetic poymer source 1..20 mol_type other RNA organism synthetic construct cgtgagtaaacctgaatctt 20 RNA PAT misc_feature 1..20 note synthetic poymer source 1..20 mol_type other RNA organism synthetic construct gatattggcataagcctccc 20 RNA PAT misc_feature 1..20 note synthetic poymer source 1..20 mol_type other RNA organism synthetic construct agagtctctcagctggtaca 000 12 RNA PAT misc_feature 1..12 note synthetic poymer source 1..12 mol_type other RNA organism synthetic construct gttttagagcta 000 63 RNA PAT misc_feature 1..63 note synthetic poymer source 1..63 mol_type other RNA organism synthetic construct tagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttt 99 RNA PAT misc_feature 1..99 note synthetic poymer misc_feature 1..20 note n is a, c, g, or u source 1..99 mol_type other RNA organism synthetic construct nnnnnnnnnnnnnnnnnnnngttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttt 000 000 000 000 000 000 000 000 000 000 000 000 000 000 000 000 42 AA PAT REGION 1..42 note Synthetic hinge domain source 1..42 mol_type protein organism synthetic construct AAAIEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKP 28 AA PAT REGION 1..28 note Synthetic transmembrane domain source 1..28 mol_type protein organism synthetic construct MFWVLVVVGGVLACYSLLVTVAFIIFWV 112 AA PAT REGION 1..112 note Synthetic signaling domain source 1..112 mol_type protein organism synthetic construct RVKFSRSADAPAYKQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 1467 DNA PAT misc_feature 1..1467 note Synthetic nucleotide sequence source 1..1467 mol_type other DNA organism synthetic construct atggccttaccagtgaccgccttgctcctgccgctggccttgctgctccacgccgccaggccggacatccagatgacacagactacatcctccctgtctgcctctctgggagacagagtcaccatcagttgcagggcaagtcaggacattagtaaatatttaaattggtatcagcagaaaccagatggaactgttaaactcctgatctaccatacatcaagattacactcaggagtcccatcaaggttcagtggcagtgggtctggaacagattattctctcaccattagcaacctggagcaagaagatattgccacttacttttgccaacagggtaatacgcttccgtacacgttcggaggggggaccaagctggagatcacaggctccacctctggatccggcaagcccggatctggcgagggatccaccaagggcgaggtgaaactgcaggagtcaggacctggcctggtggcgccctcacagagcctgtccgtcacatgcactgtctcaggggtctcattacccgactatggtgtaagctggattcgccagcctccacgaaagggtctggagtggctgggagtaatatggggtagtgaaaccacatactataattcagctctcaaatccagactgaccatcatcaaggacaactccaagagccaagttttcttaaaaatgaacagtctgcaaactgatgacacagccatttactactgtgccaaacattattactacggtggtagctatgctatggactactggggccaaggaacctcagtcaccgtctcctcaaccacgacgccagcgccgcgaccaccaacaccggcgcccaccatcgcgtcgcagcccctgtccctgcgcccagaggcgtgccggccagcggcggggggcgcagtgcacacgagggggctggacttcgcctgtgatatctacatctgggcgcccttggccgggacttgtggggtccttctcctgtcactggttatcaccctttactgcaaacggggcagaaagaaactcctgtatatattcaaacaaccatttatgagaccagtacaaactactcaagaggaagatggctgtagctgccgatttccagaagaagaagaaggaggatgtgaactgagagtgaagttcagcaggagcgcagacgcccccgcgtaccagcagggccagaaccagctctataacgagctcaatctaggacgaagagaggagtacgatgttttggacaagagacgtggccgggaccctgagatggggggaaagccgagaaggaagaaccctcaggaaggcctgtacaatgaactgcagaaagataagatggcggaggcctacagtgagattgggatgaaaggcgagcgccggaggggcaaggggcacgatggcctttaccagggtctcagtacagccaccaaggacacctacgacgcccttcacatgcaggccctgccccctcgc 489 AA PAT REGION 1..489 note Synthetic amino acid source 1..489 mol_type protein organism synthetic construct MALPVTALLLPLALLLHAARPDIQMTQTTSSLSASLGDRVTISCRASQDISKYLNWYQQKPDGTVKLLIYHTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQGNTLPYTFGGGTKLEITGSTSGSGKPGSGEGSTKGEVKLQESGPGLVAPSQSLSVTCTVSGVSLPDYGVSWIRQPPRKGLEWLGVIWGSETTYYNSALKSRLTIIKDNSKSQVFLKMNSLQTDDTAIYYCAKHYYYGGSYAMDYWGQGTSVTVSSTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 243 AA PAT REGION 1..243 note Synthetic amino acid source 1..243 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQKYDLLTFGGGTKVEIKGSTSGSGKPGSGEGSTKGQLQLQESGPGLVKPSETLSLTCTVSGGSISSSSYYWGWIRQPPGKGLEWIGSISYSGSTYYNPSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCARDRGDTILDVWGQGTMVTVSS 106 AA PAT REGION 1..106 note Synthetic amino acid source 1..106 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQKYDLLTFGGGTKVEIK 6 AA PAT REGION 1..6 note Synthetic amino acid source 1..6 mol_type protein organism synthetic construct QSISSY 000 8 AA PAT REGION 1..8 note Synthetic amino acid source 1..8 mol_type protein organism synthetic construct QQKYDLLT 119 AA PAT REGION 1..119 note Synthetic amino acid source 1..119 mol_type protein organism synthetic construct QLQLQESGPGLVKPSETLSLTCTVSGGSISSSSYYWGWIRQPPGKGLEWIGSISYSGSTYYNPSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCARDRGDTILDVWGQGTMVTVSS 10 AA PAT REGION 1..10 note Synthetic amino acid source 1..10 mol_type protein organism synthetic construct GGSISSSSYY 7 AA PAT REGION 1..7 note Synthetic amino acid source 1..7 mol_type protein organism synthetic construct ISYSGST 11 AA PAT REGION 1..11 note Synthetic amino acid source 1..11 mol_type protein organism synthetic construct ARDRGDTILDV 1503 DNA PAT misc_feature 1..1503 note Synthetic nucleotide sequence source 1..1503 mol_type other DNA organism synthetic construct atggccttaccagtgaccgccttgctcctgccgctggccttgctgctccacgccgccaggccggacatccagatgacccagtctccatcctccctgtctgcatctgtaggagacagagtcaccatcacttgccgggcaagtcagagcattagcagctatttaaattggtatcagcagaaaccagggaaagcccctaagctcctgatctatgctgcatccagtttgcaaagtggggtcccatcaaggttcagtggcagtggatctgggacagatttcactctcaccatcagcagtctgcaacctgaagattttgcaacttactactgtcagcaaaaatacgacctcctcacttttggcggagggaccaaggttgagatcaaaggcagcaccagcggctccggcaagcctggctctggcgagggcagcacaaagggacagctgcagctgcaggagtcgggcccaggactggtgaagccttcggagaccctgtccctcacctgcactgtctctggtggctccatcagcagtagtagttactactggggctggatccgccagcccccagggaaggggctggagtggattgggagtatctcctatagtgggagcacctactacaacccgtccctcaagagtcgagtcaccatatccgtagacacgtccaagaaccagttctccctgaagctgagttctgtgaccgccgcagacacggcggtgtactactgcgccagagatcgtggagacaccatactagacgtatggggtcagggtacaatggtcaccgtcagctcattcgtgcccgtgttcctgcccgccaaacctaccaccacccctgcccctagacctcccaccccagccccaacaatcgccagccagcctctgtctctgcggcccgaagcctgtagacctgctgccggcggagccgtgcacaccagaggcctggacttcgcctgcgacatctacatctgggcccctctggccggcacctgtggcgtgctgctgctgagcctggtgatcaccctgtactgcaaccaccggaacaaacggggcagaaagaaactcctgtatatattcaaacaaccatttatgagaccagtacaaactactcaagaggaagatggctgtagctgccgatttccagaagaagaagaaggaggatgtgaactgagagtgaagttcagcagatccgccgacgcccctgcctaccagcagggacagaaccagctgtacaacgagctgaacctgggcagacgggaagagtacgacgtgctggacaagcggagaggccgggaccccgagatgggcggaaagcccagacggaagaacccccaggaaggcctgtataacgaactgcagaaagacaagatggccgaggcctacagcgagatcggcatgaagggcgagcggaggcgcggcaagggccacgatggcctgtaccagggcctgagcaccgccaccaaggacacctacgacgccctgcacatgcaggccctgccccccaga 501 AA PAT REGION 1..501 note Synthetic amino acid source 1..501 mol_type protein organism synthetic construct MALPVTALLLPLALLLHAARPDIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQKYDLLTFGGGTKVEIKGSTSGSGKPGSGEGSTKGQLQLQESGPGLVKPSETLSLTCTVSGGSISSSSYYWGWIRQPPGKGLEWIGSISYSGSTYYNPSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCARDRGDTILDVWGQGTMVTVSSFVPVFLPAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCNHRNKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 305 AA PAT REGION 1..305 note Human CD47 (without signal sequence) (aa 19-323) source 1..305 mol_type protein organism synthetic construct QLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPNENILIVIFPIFAILLFWGQFGIKTLKYRSGGMDEKTIALLVAGLVITVIVIVGAILFVPGEYSLKNATGLGLIVTSTGILILLHYYVFSTAIGLTSFVIAILVIQVIAYILAVVGLSLCIAACIPMHGPLLISGLSILALAQLLGLVYMKFVASNQKTIQPPRKAVEEPLNAFKESKGMMNDE 323 AA PAT REGION 1..323 note Human CD47 (with signal sequence) (aa 1-323) source 1..323 mol_type protein organism synthetic construct MWPLVAALLLGSACCGSAQLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPNENILIVIFPIFAILLFWGQFGIKTLKYRSGGMDEKTIALLVAGLVITVIVIVGAILFVPGEYSLKNATGLGLIVTSTGILILLHYYVFSTAIGLTSFVIAILVIQVIAYILAVVGLSLCIAACIPMHGPLLISGLSILALAQLLGLVYMKFVASNQKTIQPPRKAVEEPLNAFKESKGMMNDE