WO 26013-20004.40 US 63/342,975 2022-05-17 Umoja Biopharma, Inc. MANUFACTURING VIRAL PARTICLES 130 250 AA PAT REGION 1..250 note CD58 source 1..250 mol_type protein organism synthetic construct MVAGSDAGRALGVLSVVCLLHCFGFISCFSQQIYGVVYGNVTFHVPSNVPLKEVLWKKQKDKVAELENSEFRAFSSFKNRVYLDTVSGSLTIYNLTSSDEDEYEMESPNITDTMKFFLYVLESLPSPTLTCALTNGSIEVQCMIPEHYNSHRGLIMYSWDCPMEQCKRNSTSIYFKMENDLPQKIQCTLSNPLFNTTSSIILTTCIPSSGHSRHRYALIPIPLAVITTCIVLYMNGILKCDRKPDRTNSN 750 DNA PAT misc_feature 1..750 note CD58 source 1..750 mol_type other DNA organism synthetic construct atggttgctgggagcgacgcggggcgggccctgggggtcctcagcgtggtctgcctgctgcactgctttggtttcatcagctgtttttcccaacaaatatatggtgttgtgtatgggaatgtaactttccatgtaccaagcaatgtgcctttaaaagaggtcctatggaaaaaacaaaaggataaagttgcagaactggaaaattctgagttcagagctttctcatcttttaaaaatagggtttatttagacactgtgtcaggtagcctcactatctacaacttaacatcatcagatgaagatgagtatgaaatggaatcgccaaatattactgataccatgaagttctttctttatgtgcttgagtctcttccatctcccacactaacttgtgcattgactaatggaagcattgaagtccaatgcatgataccagagcattacaacagccatcgaggacttataatgtactcatgggattgtcctatggagcaatgtaaacgtaactcaaccagtatatattttaagatggaaaatgatcttccacaaaaaatacagtgtactcttagcaatccattatttaatacaacatcatcaatcattttgacaacctgtatcccaagcagcggtcattcaagacacagatatgcacttatacccataccattagcagtaattacaacatgtattgtgctgtatatgaatggtattctgaaatgtgacagaaaaccagacagaaccaactccaat 288 AA PAT REGION 1..288 note CD80 source 1..288 mol_type protein organism synthetic construct MGHTRRQGTSPSKCPYLNFFQLLVLAGLSHFCSGVIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDNLLPSWAITLISVNGIFVICCLTYCFAPRCRERRRNERLRRESVRPV 864 DNA PAT misc_feature 1..864 note CD80 source 1..864 mol_type other DNA organism synthetic construct atgggccacacacggaggcagggaacatcaccatccaagtgtccatacctcaatttctttcagctcttggtgctggctggtctttctcacttctgttcaggtgttatccacgtgaccaaggaagtgaaagaagtggcaacgctgtcctgtggtcacaatgtttctgttgaagagctggcacaaactcgcatctactggcaaaaggagaagaaaatggtgctgactatgatgtctggggacatgaatatatggcccgagtacaagaaccggaccatctttgatatcactaataacctctccattgtgatcctggctctgcgcccatctgacgagggcacatacgagtgtgttgttctgaagtatgaaaaagacgctttcaagcgggaacacctggctgaagtgacgttatcagtcaaagctgacttccctacacctagtatatctgactttgaaattccaacttctaatattagaaggataatttgctcaacctctggaggttttccagagcctcacctctcctggttggaaaatggagaagaattaaatgccatcaacacaacagtttcccaagatcctgaaactgagctctatgctgttagcagcaaactggatttcaatatgacaaccaaccacagcttcatgtgtctcatcaagtatggacatttaagagtgaatcagaccttcaactggaatacaaccaagcaagagcattttcctgataacctgctcccatcctgggccattaccttaatctcagtaaatggaatttttgtgatatgctgcctgacctactgctttgccccaagatgcagagagagaaggaggaatgagagattgagaagggaaagtgtacgccctgta 329 AA PAT REGION 1..329 note CD86 source 1..329 mol_type protein organism synthetic construct MDPQCTMGLSNILFVMAFLLSGAAPLKIQAYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGVMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLSSPFSIELEDPQPPPDHIPWITAVLPTVIICVMVFCLILWKWKKKKRPRNSYKCGTNTMEREESEQTKKREKIHIPERSDEAQRVFKSSKTSSCDKSDTCF 987 DNA PAT misc_feature 1..987 note CD86 source 1..987 mol_type other DNA organism synthetic construct atggatccccagtgcactatgggactgagtaacattctctttgtgatggccttcctgctctctggtgctgctcctctgaagattcaagcttatttcaatgagactgcagacctgccatgccaatttgcaaactctcaaaaccaaagcctgagtgagctagtagtattttggcaggaccaggaaaacttggttctgaatgaggtatacttaggcaaagagaaatttgacagtgttcattccaagtatatgggccgcacaagttttgattcggacagttggaccctgagacttcacaatcttcagatcaaggacaagggcttgtatcaatgtatcatccatcacaaaaagcccacaggaatgattcgcatccaccagatgaactctgaactgtcagtgcttgctaacttcagtcaacctgaaatagtaccaatttctaatataacagaaaatgtgtacataaatttgacctgctcatctatacacggttacccagaacctaagaagatgagtgttttgctaagaaccaagaactcaactatcgagtatgatggtgttatgcagaaatctcaagataatgtcacagaactgtacgacgtttccatcagcttgtctgtttcattccctgatgttacgagcaatatgaccatcttctgtattctggaaactgacaagacgcggcttttatcttcacctttctctatagagcttgaggaccctcagcctcccccagaccacattccttggattacagctgtacttccaacagttattatatgtgtgatggttttctgtctaattctatggaaatggaagaagaagaagcggcctcgcaactcttataaatgtggaaccaacacaatggagagggaagagagtgaacagaccaagaaaagagaaaaaatccatatacctgaaaggtctgatgaagcccagcgtgtttttaaaagttcgaagacatcttcatgcgacaaaagtgatacatgtttt 208 AA PAT REGION 1..208 note CD80 Extracellular Domain source 1..208 mol_type protein organism synthetic construct VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDN 224 AA PAT REGION 1..224 note CD86 Extracellular Domain source 1..224 mol_type protein organism synthetic construct APLKIQAYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGVMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLSSPFSIELEDPQPPPDHIP 92 AA PAT REGION 1..92 note CD58 Extracellular Domain source 1..92 mol_type protein organism synthetic construct FSQQIYGVVYGNVTFHVPSNVPLKEVLWKKQKDKVAELENSEFRAFSSFKNRVYLDTVSGSLTIYNLTSSDEDEYEMESPNITDTMKFFLYV 453 AA PAT REGION 1..453 note HHLA2 Extracellular Domain source 1..453 mol_type protein organism synthetic construct QTSVSPSKVILPRGGSVLVTCSTSCDQPKLLGIETPLPKKELLLPGNNRKVYELSNVQEDSQPMCYSNCPDGQSTAKTFLTVYWTPERVELAPLPSWQPVGKNLTLRCQVEGGAPRANLTVVLLRGEKELKREPAVGEPAEVTTTVLVRRDHHGANFSCRTELDLRPQGLELFENTSAPYQLQTFVLPATPPQLVSPRVLEVDTQGTVVCSLDGLFPVSEAQVHLALGDQRLNPTVTYGNDSFSAKASVSVTAEDEGTQRLTCAVILGNQSQETLQTVTIYSFPAPNVILTKPEVSEGTEVTVKCEAHPRAKVTLNGVPAQPLGPRAQLLLKATPEDNGRSFSCSATLEVAGQLIHKNQTRELRVLYGPRLDERDCPGNWTWPENSQQTPMCQAWGNPLPELKCLKDGTFPLPIGESVTVTRDLEGTYLCRARSTQGEVTRKVTVNVLSPRYE 133 AA PAT REGION 1..133 note ICAM-1 Extracellular Domain source 1..133 mol_type protein organism synthetic construct QVSHRYPRIQSIKVQFTEYKKEKGFILTSQKEDEIMKVQNNSVIINCDGFYLISLKGYFSQEVNISLHYQKDEEPLFQLKKVRSVNSLMVASLTYKDKVYLNVTTDNTSLDDFHVNGGELILIHQNPGEFCVL 184 AA PAT REGION 1..184 note OX40-L Extracellular Domain source 1..184 mol_type protein organism synthetic construct REGPELSPDDPAGLLDLRQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVTPEIPAGLPSPRSE 173 AA PAT REGION 1..173 note 4-1BBL Extracellular Domain source 1..173 mol_type protein organism synthetic construct EPPTACREKQYLINSQCCSLCQPGQKLVSDCTEFTETECLPCGESEFLDTWNRETHCHQHKYCDPNLGLRVQQKGTSETDTICTCEEGWHCTSEACESCVLHRSCSPGFGVKQIATGVSDTICEPCPVGFFSNVSSAFEKCHPWTSCETKDLVVQQAGTNKTDVVCGPQDRLR 323 AA PAT REGION 1..323 note CD40 Extracellular Domain source 1..323 mol_type protein organism synthetic construct WPPPGTGDVVVQAPTQVPGFLGDSVTLPCYLQVPNMEVTHVSQLTWARHGESGSMAVFHQTQGPSYSESKRLEFVAARLGAELRNASLRMFGLRVEDEGNYTCLFVTFPQGSRSVDIWLRVLAKPQNTAEVQKVQLTGEPVPMARCVSTGGRPPAQITWHSDLGGMPNTSQVPGFLSGTVTVTSLWILVPSSQVDGKNVTCKVEHESFEKPQLLTVNLTVYYPPEVSISGYDNNWYLGQNEATLTCDARSNPEPTGYNWSTTMGPLPPFAVAQGAQLLIRPVDKPINTTLICNVTNALGARQAELTVQVKEGPPSEHSGISRN 155 AA PAT REGION 1..155 note CD155 Extracellular Domain source 1..155 mol_type protein organism synthetic construct QRFAQAQQQLPLESLGWDVAELQLNHTGPQQDPRLYWQGGPALGRSFLHGPELDKGQLRIHRDGIYMVHIQVTLAICSSTTASRHHPTTLAVGICSPASRSISLLRLSFHQGCTIASQRLTPLARGDTLCTNLTGTLLPSRNTDETFFGVQWVRP 161 AA PAT REGION 1..161 note CD70 Extracellular Domain source 1..161 mol_type protein organism synthetic construct LPSCKEDEYPVGSECCPKCSPGYRVKEACGELTGTVCEPCPPGTYIAHLNGLSKCLQCQMCDPAMGLRASRNCSRTENAVCGCSPGHFCIVQDGDHCAACRAYATSSPGQRVQKGGTESQDTLCQNCPPGTFSPNGTLEECQHQTKCSWLVTKAGAGTSSS 128 AA PAT REGION 1..128 note HVEM Extracellular Domain source 1..128 mol_type protein organism synthetic construct QLETAKEPCMAKFGPLPSKWQMASSEPPCVNKVSDWKLEILQNGLYLIYGQVAPNANYNDVAPFEVRLYKNKDMIQTLTNKSKIQNVGGTYELHVGDTIDLIFNSEHQVLKNNTYWGIILLANPQFIS 172 AA PAT REGION 1..172 note GITRL Extracellular Domain source 1..172 mol_type protein organism synthetic construct QRTDSIPNSPDNVPLKGGNCSEDLLCILKRAPFKKSWAYLQVAKHLNKTKLSWNKDGILHGVRYQDGNLVIQFPGLYFIICQLQFLVQCPNNSVDLKLELLINKHIKKQALVTVCESGMQTKHVYQNLSQFLLDYLQVNTTISVNVDTFQYIDTSTFPLENVLSIFLYSNSD 193 AA PAT REGION 1..193 note CD30L Extracellular Domain source 1..193 mol_type protein organism synthetic construct QGHLVHMTVVSGSNVTLNISESLPENYKQLTWFYTFDQKIVEWDSRKSKYFESKFKGRVRLDPQSGALYISKVQKEDNSTYIMRVLKKTGNEQEWKIKLQVLDPVPKPVIKIEKIEDMDDNCYLKLSCVIPGESVNYTWYGDKRPFPKELQNSVLETTLMPHNYSRCYTCQVSNSVSSKNGTVCLSPPCTLAR 407 AA PAT REGION 1..407 note SLAMF2 Extracellular Domain source 1..407 mol_type protein organism synthetic construct KDSAPTVVSGILGGSVTLPLNISVDTEIENVIWIGPKNALAFARPKENVTIMVKSYLGRLDITKWSYSLCISNLTLNDAGSYKAQINQRNFEVTTEEEFTLFVYEQLQEPQVTMKSVKVSENFSCNITLMCSVKGAEKSVLYSWTPREPHASESNGGSILTVSRTPCDPDLPYICTAQNPVSQRSSLPVHVGQFCTDPGASRGGTTGETVVGVLGEPVTLPLALPACRDTEKVVWLFNTSIISKEREEAATADPLIKSRDPYKNRVWVSSQDCSLKISQLKIEDAGPYHAYVCSEASSVTSMTHVTLLIYRRLRKPKITWSLRHSEDGICRISLTCSVEDGGNTVMYTWTPLQKEAVVSQGESHLNVSWRSSENHPNLTCTASNPVSRSSHQFLSENICSGPERNTK 204 AA PAT REGION 1..204 note Ly-9 Extracellular Domain source 1..204 mol_type protein organism synthetic construct KDSEIFTVNGILGESVTFPVNIQEPRQVKIIAWTSKTSVAYVTPGDSETAPVVTVTHRNYYERIHALGPNYNLVISDLRMEDAGDYKADINTQADPYTTTKRYNLQIYRRLGKPKITQSLMASVNSTCNVTLTCSVEKEEKNVTYNWSPLGEEGNVLQIFQTPEDQELTYTCTAQNPVSNNSDSISARQLCADIAMGFRTHHTG 204 AA PAT REGION 1..204 note CD84 Extracellular Domain source 1..204 mol_type protein organism synthetic construct QSSLTPLMVNGILGESVTLPLEFPAGEKVNFITWLFNETSLAFIVPHETKSPEIHVTNPKQGKRLNFTQSYSLQLSNLKMEDTGSYRAQISTKTSAKLSSYTLRILRQLRNIQVTNHSQLFQNMTCELHLTCSVEDADDNVSFRWEALGNTLSSQPNLTVSWDPRISSEQDYTCIAENAVSNLSFSVSAQKLCEDVKIQYTDTK 283 AA PAT REGION 1..283 note Ly108 Extracellular Domain source 1..283 mol_type protein organism synthetic construct EPHSLRYNLTVLSWDGSVQSGFLTEVHLDGQPFLRCDRQKCRAKPQGQWAEDVLGNKTWDRETRDLTGNGKDLRMTLAHIKDQKEGLHSLQEIRVCEIHEDNSTRSSQHFYYDGELFLSQNLETKEWTMPQSSRAQTLAMNVRNFLKEDAMKTKTHYHAMHADCLQELRRYLKSGVVLRRTVPPMVNVTRSEASEGNITVTCRASGFYPWNITLSWRQDGVSLSHDTQQWGDVLPDGNGTYQTWVATRICQGEEQRFTCYMEHSGNHSTHPVPSGKVLVLQSH 243 AA PAT REGION 1..243 note MICA Extracellular Domain source 1..243 mol_type protein organism synthetic construct EPHSLRYNLMVLSQDGSVQSGFLAEGHLDGQPFLRYDRQKRRAKPQGQWAEDVLGAETWDTETEDLTENGQDLRRTLTHIKDQKGVPQSSRAQTLAMNVTNFWKEDAMKTKTHYRAMQADCLQKLQRYLKSGVAIRRTVPPMVNVTCSEVSEGNITVTCRASSFYPRNITLTWRQDGVSLSHNTQQWGDVLPDGNGTYQTWVATRIRQGEEQRFTCYMEHSGNHGTHPVPSGKALVLQSQRTD 244 AA PAT REGION 1..244 note MICB Extracellular Domain source 1..244 mol_type protein organism synthetic construct MAAAASPAFLLCLPLLHLLSGWSRAGWVDTHCLCYDFIITPKSRPEPQWCEVQGLVDERPFLHYDCVNHKAKAFASLGKKVNVTKTWEEQTETLRDVVDFLKGQLLDIQVENLIPIEPLTLQARMSCEHEAHGHGRGSWQFLFNGQKFLLFDSNNRKWTALHPGAKKMTEKWEKNRDVTMFFQKISLGDCKMWLEEFLMYWEQMLDPTKPPSLAPGTTQPKAMATTLSPWSLLIIFLCFILAGR 246 AA PAT REGION 1..246 note ULBP1 source 1..246 mol_type protein organism synthetic construct MAAAAATKILLCLPLLLLLSGWSRAGRADPHSLCYDITVIPKFRPGPRWCAVQGQVDEKTFLHYDCGNKTVTPVSPLGKKLNVTTAWKAQNPVLREVVDILTEQLRDIQLENYTPKEPLTLQARMSCEQKAEGHSSGSWQFSFDGQIFLLFDSEKRMWTTVHPGARKMKEKWENDKVVAMSFHYFSMGDCIGWLEDFLMGMDSTLEPSAGAPLAMSSGTTQLRATATTLILCCLLIILPCFILPGI 244 AA PAT REGION 1..244 note ULBP2 source 1..244 mol_type protein organism synthetic construct MAAAASPAILPRLAILPYLLFDWSGTGRADAHSLWYNFTIIHLPRHGQQWCEVQSQVDQKNFLSYDCGSDKVLSMGHLEEQLYATDAWGKQLEMLREVGQRLRLELADTELEDFTPSGPLTLQVRMSCECEADGYIRGSWQFSFDGRKFLLFDSNNRKWTVVHAGARRMKEKWEKDSGLTTFFKMVSMRDCKSWLRDFLMHRKKRLEPTAPPTMAPGLAQPKAIATTLSPWSFLIILCFILPGI 195 AA PAT REGION 1..195 note ULBP3 source 1..195 mol_type protein organism synthetic construct HSLCFNFTIKSLSRPGQPWCEAQVFLNKNLFLQYNSDNNMVKPLGLLGKKVYATSTWGELTQTLGEVGRDLRMLLCDIKPQIKTSDPSTLQVEMFCQREAERCTGASWQFATNGEKSLLFDAMNMTWTVINHEASKIKETWKKDRGLEKYFRKLSKGDCDHWLREFLGHWEAMPEPTVSPVNASDIHWSSSSLPD 198 AA PAT REGION 1..198 note ULBP4 Extracellular Domain source 1..198 mol_type protein organism synthetic construct GLADPHSLCYDITVIPKFRPGPRWCAVQGQVDEKTFLHYDCGSKTVTPVSPLGKKLNVTTAWKAQNPVLREVVDILTEQLLDIQLENYIPKEPLTLQARMSCEQKAEGHGSGSWQLSFDGQIFLLFDSENRMWTTVHPGARKMKEKWENDKDMTMSFHYISMGDCTGWLEDFLMGMDSTLEPSAGAPPTMSSGTAQPR 246 AA PAT REGION 1..246 note ULBP5 Extracellular Domain source 1..246 mol_type protein organism synthetic construct MAAAAIPALLLCLPLLFLLFGWSRARRDDPHSLCYDITVIPKFRPGPRWCAVQGQVDEKTFLHYDCGNKTVTPVSPLGKKLNVTMAWKAQNPVLREVVDILTEQLLDIQLENYTPKEPLTLQARMSCEQKAEGHSSGSWQFSIDGQTFLLFDSEKRMWTTVHPGARKMKEKWENDKDVAMSFHYISMGDCIGWLEDFLMGMDSTLEPSAGAPLAMSSGTTQLRATATTLILCCLLIILPCFILPGI 238 AA PAT REGION 1..238 note ULBP6 source 1..238 mol_type protein organism synthetic construct DTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSVEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWINKTDNSLLDQALQNDTVFLNMRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVSTGEKNAAT 238 AA PAT REGION 1..238 note B7-H2 Extracellular Domain source 1..238 mol_type protein organism synthetic construct DLKVEMMAGGTQITPLNDNVTIFCNIFYSQPLNITSMGITWFWKSLTFDKEVKVFEFFGDHQEAFRPGAIVSPWRLKSGDASLRLPGIQLEEAGEYRCEVVVTPLKAQGTVQLEVVASPASRLLLDQVGMKENEDKYMCESSGFYPEAINITWEKQTQKFPHPIEISEDVITGPTIKNMDGTFNVTSCLKLNSSQEDPGTVYQCVVRHASLHTPLRSNFTLTAARHSLSETEKTDNFS 162 AA PAT REGION 1..162 note B7-H6 Extracellular Domain source 1..162 mol_type protein organism synthetic construct FKVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYPSSSQDSENITAA 220 AA PAT REGION 1..220 note B7-H5 Extracellular Domain source 1..220 mol_type protein organism synthetic construct LEVQVPEDPVVALVGTDATLCCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFAEGQDQGSAYANRTALFPDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYRGYPEAEVFWQDGQGVPLTGNVTTSQMANEQGLFDVHSVLRVVLGANGTYSCLVRNPVLQQDAHGSVTITGQPMTFPPEA 244 AA PAT REGION 1..244 note B7-H3 Extracellular Domain source 1..244 mol_type protein organism synthetic construct LIIGFGISGRHSITVTTVASAGNIGEDGILSCTFEPDIKLSDIVIQWLKEGVLGLVHEFKEGKDELSEQDEMFRGRTAVFADQVIVGNASLRLKNVQLTDAGTYKCYIITSKGKGNANLEYKTGAFSMPEVNVDYNASSETLRCEAPRWFPQPTVVWASQVDQGANFSEVSNTSFELNSENVTMKVVSVLYNVTINNTYSCMIENDIAKATGDIKVTESEIKRRSHLQLLNSKASTTENLYFQG 128 AA PAT REGION 1..128 note B7x Extracellular Domain source 1..128 mol_type protein organism synthetic construct LSVQQGPNLLQVRQGSQATLVCQVDQATAWERLRVKWTKDGAILCQPYITNGSLSLGVCGPQGRLSWQAPSHLTLQLDPVSLNHSGAYVCWAAVEIPELEEAEGNITRLFVDPDDPTQNRNRIASFPG 133 AA PAT REGION 1..133 note TMIGD2 Extracellular Domain source 1..133 mol_type protein organism synthetic construct APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT 152 AA PAT REGION 1..152 note IL-2 source 1..152 mol_type protein organism synthetic construct DCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRAARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGEAQPTKSLEENKSLKEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEH 197 AA PAT REGION 1..197 note IL-7 source 1..197 mol_type protein organism synthetic construct RNLPVATPDPGMFPCLHHSQNLLRAVSNMLQKARQTLEFYPCTSEEIDHEDITKDKTSTVEACLPLELTKNESCLNSRETSFITNGSCLASRKTSFMMALCLSSIYEDLKMYQVEFKTMNAKLLMDPKRQIFLDQNMLAVIDELMQALNFNSETVPQKSSLEEPDFYKTKIKLCILLHAFRIRAVTIDRVMSYLNAS 306 AA PAT REGION 1..306 note IL-12 subunit alpha source 1..306 mol_type protein organism synthetic construct IWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS 114 AA PAT REGION 1..114 note IL-12 subunit beta source 1..114 mol_type protein organism synthetic construct NWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVENLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS 157 AA PAT REGION 1..157 note IL-15 source 1..157 mol_type protein organism synthetic construct YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED 138 AA PAT REGION 1..138 note IL-18 source 1..138 mol_type protein organism synthetic construct HKSSSQGQDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANTGNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIHQHLSSRTHGSEDS 453 AA PAT REGION 1..453 note IL-21 source 1..453 mol_type protein organism synthetic construct QTSVSPSKVILPRGGSVLVTCSTSCDQPKLLGIETPLPKKELLLPGNNRKVYELSNVQEDSQPMCYSNCPDGQSTAKTFLTVYWTPERVELAPLPSWQPVGKNLTLRCQVEGGAPRANLTVVLLRGEKELKREPAVGEPAEVTTTVLVRRDHHGANFSCRTELDLRPQGLELFENTSAPYQLQTFVLPATPPQLVSPRVLEVDTQGTVVCSLDGLFPVSEAQVHLALGDQRLNPTVTYGNDSFSAKASVSVTAEDEGTQRLTCAVILGNQSQETLQTVTIYSFPAPNVILTKPEVSEGTEVTVKCEAHPRAKVTLNGVPAQPLGPRAQLLLKATPEDNGRSFSCSATLEVAGQLIHKNQTRELRVLYGPRLDERDCPGNWTWPENSQQTPMCQAWGNPLPELKCLKDGTFPLPIGESVTVTRDLEGTYLCRARSTQGEVTRKVTVNVLSPRYE 327 DNA PAT misc_feature 1..327 note E2 scFv VL nucleotide source 1..327 mol_type other DNA organism synthetic construct tccgtgctgacccagcctagctccgtgtctgccgcaccaggacagaaggtgacaatcagctgttccggctctaccagcaacatcggcaacaattacgtgagctggtaccagcagcaccctggcaaggccccaaagctgatgatctacgacgtgtccaagaggccatctggagtgcctgatcggttctccggctctaagagcggcaattccgcctctctggacatcagcggactgcagtccgaggacgaggcagattactattgcgccgcctgggacgatagcctgtccgagtttctgttcggcaccggcacaaagctgaccgtgctg 500 AA PAT REGION 1..500 note Gag polypeptide source 1..500 mol_type protein organism synthetic construct MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFAVNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKIEEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPGATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEGHIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAPPEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ 109 AA PAT REGION 1..109 note E2 scFv VL polypeptide source 1..109 mol_type protein organism synthetic construct SVLTQPSSVSAAPGQKVTISCSGSTSNIGNNYVSWYQQHPGKAPKLMIYDVSKRPSGVPDRFSGSKSGNSASLDISGLQSEDEADYYCAAWDDSLSEFLFGTGTKLTVL 381 DNA PAT misc_feature 1..381 note E2 scFv VH nucleotide source 1..381 mol_type other DNA organism synthetic construct caggtgcagctggtggagagcggaggaaacctggtgcagccaggaggctccctgcgcctgtcttgtgccgccagcggctttaccttcggctcttttagcatgtcctgggtgcgccaggcacctggaggaggactggagtgggtggccggcctgagcgcccggtctagcctgacacactatgccgactccgtgaagggccgcttcaccatctcccgggataacgccaagaatagcgtgtacctgcagatgaatagcctgcgggtggaggacacagccgtgtactattgcgccaggcgctcctatgattcctctggctactggggccacttttactcttatatggacgtgtggggacagggcaccctggtgacagtgagctcc 1003 AA PAT REGION 1..1003 note Pol polypeptide source 1..1003 mol_type protein organism synthetic construct FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAGADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIGGIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHTNDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQAIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAHKGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVASCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQETAYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVASRQDED 127 AA PAT REGION 1..127 note E2 scFv VH polypeptide source 1..127 mol_type protein organism synthetic construct QVQLVESGGNLVQPGGSLRLSCAASGFTFGSFSMSWVRQAPGGGLEWVAGLSARSSLTHYADSVKGRFTISRDNAKNSVYLQMNSLRVEDTAVYYCARRSYDSSGYWGHFYSYMDVWGQGTLVTVSS 54 DNA PAT misc_feature 1..54 note E2 scFv linker nucleotide source 1..54 mol_type other DNA organism synthetic construct ggctctacaagcggatccggcaagccaggatctggagagggcagcacaaaggga 4307 DNA PAT misc_feature 1..4307 note Gag-pol nucleotide source 1..4307 mol_type other DNA organism synthetic construct atgggcgcccgcgccagcgtgctttctggcggcgagctggacaggtgggagaagattcgcctgcggcctggaggaaagaaaaagtacaagctgaagcacatcgtgtgggcttctcgggaactggaaagattcgccgtgaaccctggactgctagagacctccgaaggctgcagacagatcctgggacagctgcaacctagcctgcagaccggcagcgaggagctgagaagcctgtacaacaccgtcgccaccctgtattgtgtgcaccaaagaatcgagatcaaggacaccaaggaggctctggataagatcgaggaagagcagaacaagagcaagaaaaaagcccagcaggccgccgctgataccggccattctaatcaggtgtcccagaactaccccattgtgcaaaatatccagggccagatggtccaccaggccatcagccctagaaccctgaatgcctgggtgaaggtggtggaagagaaggccttttctccagaggtgatccctatgttcagcgccctgagcgagggcgctacccctcaggacctgaacacaatgctgaacaccgtgggcggccaccaggccgccatgcagatgctgaaagaaaccatcaacgaggaggccgccgaatgggaccgggtgcaccccgttcacgccggcccaatcgcccctggccagatgcgggaacctagaggcagcgacatcgccggcacaaccagcacactgcaagagcagatcggatggatgacacacaacccccccatccccgtgggcgaaatctacaagcggtggatcattctgggactgaacaaaatcgttagaatgtacagccctaccagcatcctggatatcagacagggcccaaaggagcctttccgggactacgtggacagattttacaagaccctgagagccgaacaggcctcccaagaggtgaagaactggatgacagagaccctgctggtgcagaatgccaaccctgattgtaagacaatcctgaaggccctcggacctggcgctacactggaagaaatgatgaccgcctgccagggcgtgggcggccccggccacaaggccagagtgctggccgaggctatgagccaggtgacaaaccccgccacaatcatgatccagaagggcaacttcagaaaccagcggaagaccgtgaaatgcttcaactgcggcaaggaaggccacatcgcaaagaactgcagagcccctaggaagaaaggctgttggaagtgcggaaaggaaggacaccaaatgaaagattgtactgagagacaggctaattttttagggaagatctggccttcccacaagggaaggccagggaattttcttcagagcagaccagagccaacagccccaccagaagagagcttcaggtttggggaagagacaacaactccctctcagaagcaggagccgatagacaaggaactgtatcctttagcttccctcagatcactctttggcagcgacccctcgtcacaataaagatcggcggacagctgaaagaggccttgctggacaccggagccgatgacaccgtgctggaagaaatgaacctgcctggaagatggaaacctaagatgatcggtggcatcggcggatttatcaaagtgcgacagtatgaccagatcctgatcgagatttgcggccacaaagctatcggaacagtgctggtcggcccgacccccgtgaacatcattggccgcaacctgctgacacagatcggttgtacactgaactttcctatcagccctatcgaaaccgtgccggtcaagctgaagcccggcatggatggccctaaggtgaagcagtggcccctgacagaggaaaagatcaaggcactggtggaaatctgcacagaaatggaaaaagagggcaagatttctaaaatcggcccagagaacccctacaacacccctgttttcgccatcaagaagaaagattccaccaagtggaggaagctggtggactttcgggaactgaacaagcggacccaggatttctgggaggtgcagctgggcatcccccaccctgccggcctgaaacaaaaaaaaagcgtgaccgtgctggacgtgggcgacgcctatttcagcgtgcctctggataaggacttccggaaatacaccgcctttaccatccctagcatcaacaacgagacccctggcatccggtaccagtacaacgtgctcccacagggctggaagggctcacccgccatcttccagtgcagcatgaccaagatcctggagcctttcagaaagcagaatcctgacatcgtgatctaccagtacatggacgacctgtacgtgggctctgatctggagatcggacagcacagaacaaagatcgaagagctgagacagcatctgctgagatggggtttcaccacccccgacaagaagcaccagaaggaacctccttttctgtggatgggctacgagctgcaccccgataagtggacagtgcagcccatcgtgctgcccgagaaggactcctggaccgtgaacgacattcagaagctggtcggaaagctgaattgggcttcccaaatctacgccggcatcaaggtgcggcagctgtgcaagcttctgcgcggcacaaaggccctgacggaagtcgtgccactgaccgaggaagccgaattagagctggccgaaaacagagaaattctgaaagaacctgtgcacggcgtttactacgacccttctaaggacctgatcgccgaaatccagaaacaaggccagggccagtggacttaccaaatctaccaggagcctttcaaaaacctcaagaccggcaagtacgccagaatgaagggagcccatacaaacgacgtgaagcagctgacagaggctgttcagaagatcgccacagaaagcatcgtgatctggggcaagaccccaaagttcaagctgcctatccaaaaggaaacctgggaggcctggtggaccgagtactggcaggccacctggattcctgaatgggagttcgtgaacacaccacctcttgtgaagctgtggtaccagctggaaaaggagccaatcatcggcgccgagacattctacgtggacggcgccgccaaccgggagaccaaactgggaaaggccggatatgtgaccgacagaggcagacaaaaggtggtgcctctgaccgatacaactaaccagaaaacagagctgcaggccattcacctggccctgcaagacagcggcctggaagtgaatatcgtgacagacagtcagtacgccctgggcatcatccaggctcagcctgacaagagcgagagcgagctggtgtcccagatcatcgagcagctgatcaaaaaggagaaggtttatctggcctgggtgcccgcccacaagggcatcggaggcaacgagcaggtggacaagctggtcagcgccggcatccggaaggtgctgttcctggacggcatcgacaaggcccaggaggaacacgagaagtaccacagcaactggcgggccatggccagcgacttcaacctgccacctgtggttgctaaggagatcgtcgcctcttgtgataagtgccagctgaagggcgaggccatgcacggccaagtggattgcagccctgggatctggcagctagactgtacccacctggagggcaaggtgatcctggtggcagtgcacgtggccagcggctacatcgaggctgaggtgatccccgccgaaacgggccaggagaccgcctactttctgctgaagctagccggccggtggcctgtgaagaccgtgcacaccgataacggcagcaatttcaccagcacaaccgtgaaggctgcctgctggtgggctggaatcaagcaggagttcggcatcccatacaatcctcagtctcagggcgtgatcgagagcatgaacaaggaactgaagaagatcattggtcaggtcagagatcaggccgagcacctgaaaaccgccgttcaaatggctgtgttcatccataacttcaaaagaaaaggcggcatcggcggctacagcgccggcgaaagaatcgtggatatcatcgcgaccgacatccaaacaaaagagctgcaaaagcaaatcaccaagatccagaacttcagagtgtactacagagatagcagagatcctgtgtggaagggacctgccaagctgctgtggaagggcgagggcgccgtggtgatccaggacaatagcgacatcaaggtcgtgcccagaagaaaggctaaaatcattagagactacggcaaacagatggccggagatgattgcgtggcttctagacaggacgaggactga 18 AA PAT REGION 1..18 note E2 scFv linker polypeptide source 1..18 mol_type protein organism synthetic construct GSTSGSGKPGSGEGSTKG 762 DNA PAT misc_feature 1..762 note E2 scFv nucleotide source 1..762 mol_type other DNA organism synthetic construct tccgtgctgacccagcctagctccgtgtctgccgcaccaggacagaaggtgacaatcagctgttccggctctaccagcaacatcggcaacaattacgtgagctggtaccagcagcaccctggcaaggccccaaagctgatgatctacgacgtgtccaagaggccatctggagtgcctgatcggttctccggctctaagagcggcaattccgcctctctggacatcagcggactgcagtccgaggacgaggcagattactattgcgccgcctgggacgatagcctgtccgagtttctgttcggcaccggcacaaagctgaccgtgctgggctctacaagcggatccggcaagccaggatctggagagggcagcacaaagggacaggtgcagctggtggagagcggaggaaacctggtgcagccaggaggctccctgcgcctgtcttgtgccgccagcggctttaccttcggctcttttagcatgtcctgggtgcgccaggcacctggaggaggactggagtgggtggccggcctgagcgcccggtctagcctgacacactatgccgactccgtgaagggccgcttcaccatctcccgggataacgccaagaatagcgtgtacctgcagatgaatagcctgcgggtggaggacacagccgtgtactattgcgccaggcgctcctatgattcctctggctactggggccacttttactcttatatggacgtgtggggacagggcaccctggtgacagtgagctcc 762 DNA PAT misc_feature 1..762 note E2 scFv nucleoti de source 1..762 mol_type other DNA organism synthetic construct tccgtgctgacccagcctagctccgtgtctgccgcaccaggacagaaggtgacaatcagctgttccggctctaccagcaacatcggcaacaattacgtgagctggtaccagcagcaccctggcaaggccccaaagctgatgatctacgacgtgtccaagaggccatctggagtgcctgatcggttctccggctctaagagcggcaattccgcctctctggacatcagcggactgcagtccgaggacgaggcagattactattgcgccgcctgggacgatagcctgtccgagtttctgttcggcaccggcacaaagctgaccgtgctgggctctacaagcggatccggcaagccaggatctggagagggcagcacaaagggacaggtgcagctggtggagagcggaggaaacctggtgcagccaggaggctccctgcgcctgtcttgtgccgccagcggctttaccttcggctcttttagcatgtcctgggtgcgccaggcacctggaggaggactggagtgggtggccggcctgagcgcccggtctagcctgacacactatgccgactccgtgaagggccgcttcaccatctcccgggataacgccaagaatagcgtgtacctgcagatgaatagcctgcgggtggaggacacagccgtgtactattgcgccaggcgctcctatgattcctctggctactggggccacttttactcttatatggacgtgtggggacagggcaccctggtgacagtgagctcc 254 AA PAT REGION 1..254 note E2 scFv polypeptide source 1..254 mol_type protein organism synthetic construct SVLTQPSSVSAAPGQKVTISCSGSTSNIGNNYVSWYQQHPGKAPKLMIYDVSKRPSGVPDRFSGSKSGNSASLDISGLQSEDEADYYCAAWDDSLSEFLFGTGTKLTVLGSTSGSGKPGSGEGSTKGQVQLVESGGNLVQPGGSLRLSCAASGFTFGSFSMSWVRQAPGGGLEWVAGLSARSSLTHYADSVKGRFTISRDNAKNSVYLQMNSLRVEDTAVYYCARRSYDSSGYWGHFYSYMDVWGQGTLVTVSS 141 DNA PAT misc_feature 1..141 note CD8alpha source 1..141 mol_type other DNA organism synthetic construct accaccacacctgctcctagaccacctacacccgctcctaccatcgccagccagcctctgtctctgagacctgaggcctgtagacctgccgctggaggcgctgtgcacaccagaggactggatttcgcctgcgacatctac 47 AA PAT REGION 1..47 note CD8alpha source 1..47 mol_type protein organism synthetic construct TTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIY 13 AA PAT REGION 1..13 note CD8 Hinge source 1..13 mol_type protein organism synthetic construct GAVHTRGLDFACD 13 AA PAT REGION 1..13 note Modified IgG4 Hinge source 1..13 mol_type protein organism synthetic construct ESKYGPPSPPSPA 12 AA PAT REGION 1..12 note Modified IgG4 Hinge source 1..12 mol_type protein organism synthetic construct ESKYGPPSPPSP 12 AA PAT REGION 1..12 note IgG1 Hinge source 1..12 mol_type protein organism synthetic construct EPKSCDKTHTCP 13 AA PAT REGION 1..13 note PD1 Hinge source 1..13 mol_type protein organism synthetic construct PSPRPAGQFQTLV 13 AA PAT REGION 1..13 note CD28 Hinge source 1..13 mol_type protein organism synthetic construct LCPSPLFPGPSKP 66 DNA PAT misc_feature 1..66 note CD8 transmembrane nucleotide source 1..66 mol_type other DNA organism synthetic construct atctgggctcctctggctggaacatgcggcgtgctgctcctgagcctggtgatcacactgtactgc 22 AA PAT REGION 1..22 note CD8 transmembrane polypeptide source 1..22 mol_type protein organism synthetic construct IWAPLAGTCGVLLLSLVITLYC 126 DNA PAT misc_feature 1..126 note 41BB nucleotide source 1..126 mol_type other DNA organism synthetic construct aagcgcggccggaagaagctgctctacatctttaagcagccattcatgcgccccgtgcagaccacacaggaggaggacggctgctcctgtcggtttccagaggaggaggagggaggatgtgagctg 42 AA PAT REGION 1..42 note 41BB polypeptide source 1..42 mol_type protein organism synthetic construct KRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCEL 336 DNA PAT misc_feature 1..336 note CD3zeta nucleotide source 1..336 mol_type other DNA organism synthetic construct agagtgaagttctctaggagcgccgatgcccctgcctatcagcagggacagaaccagctgtacaacgagctgaatctgggccggagagaggagtacgacgtgctggataagaggaggggaagagacccagagatgggaggcaagcctcggagaaagaacccacaggagggcctgtataatgagctgcagaaggacaagatggccgaggcctactccgagatcggcatgaagggagagaggcgccggggcaagggacacgatggcctgtatcagggcctgagcaccgccacaaaggacacatacgatgccctgcacatgcaggccctgcctccaagg 112 AA PAT REGION 1..112 note CD3zeta polypeptide source 1..112 mol_type protein organism synthetic construct RVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 1380 DNA PAT misc_feature 1..1380 note CD19 CAR nucleotide source 1..1380 mol_type other DNA organism synthetic construct ctgctgctggtgacctccctgctgctgtgcgagctgcctcacccagcctttctgctgatccccgacatccagatgacacagaccacaagctccctgtctgccagcctgggcgacagagtgaccatctcctgtagggcctctcaggatatcagcaagtacctgaactggtatcagcagaagccagatggcacagtgaagctgctgatctaccacacctccaggctgcactctggagtgccaagccggttctccggatctggaagcggcaccgactattccctgacaatctctaacctggagcaggaggatatcgccacatacttttgccagcagggcaataccctgccatatacattcggcggaggaaccaagctggagatcaccggatccacatctggaagcggcaagccaggaagcggagagggatccacaaagggagaggtgaagctgcaggagagcggaccaggactggtggcaccatcccagtctctgagcgtgacctgtacagtgtccggcgtgtctctgcctgactacggcgtgtcctggatcaggcagccacctaggaagggactggagtggctgggcgtgatctggggctctgagaccacatactataattctgccctgaagagccgcctgaccatcatcaaggacaactccaagtctcaggtgtttctgaagatgaatagcctgcagaccgacgatacagccatctactattgcgccaagcactactattacggcggctcctacgccatggattattggggccagggcacctccgtgacagtgtctagcggcgctgtgcacaccagaggactggatttcgcctgcgacttctgggtgctggtggtggtgggaggcgtgctggcctgttactccctgctggtgaccgtggcctttatcatcttctgggtgaagagaggcaggaagaagctgctgtatatctttaagcagcccttcatgcgccctgtgcagaccacacaggaggaggacggctgcagctgtcggtttccagaggaggaggagggaggatgcgagctgcgcgtgaagttcagccggtccgccgatgcccctgcctaccagcagggccagaaccagctgtataacgagctgaatctgggccggagagaggagtacgacgtgctggataagaggaggggaagggacccagagatgggaggcaagcctcggagaaagaacccacaggagggcctgtacaatgagctgcagaaggacaagatggccgaggcctattctgagatcggcatgaagggagagaggcgccggggcaagggacacgatggcctgtaccagggcctgagcaccgccacaaaggacacatatgatgccctgcacatgcaggccctgccacctagg 460 AA PAT REGION 1..460 note CD19 CAR polypeptide source 1..460 mol_type protein organism synthetic construct LLLVTSLLLCELPHPAFLLIPDIQMTQTTSSLSASLGDRVTISCRASQDISKYLNWYQQKPDGTVKLLIYHTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQGNTLPYTFGGGTKLEITGSTSGSGKPGSGEGSTKGEVKLQESGPGLVAPSQSLSVTCTVSGVSLPDYGVSWIRQPPRKGLEWLGVIWGSETTYYNSALKSRLTIIKDNSKSQVFLKMNSLQTDDTAIYYCAKHYYYGGSYAMDYWGQGTSVTVSSGAVHTRGLDFACDFWVLVVVGGVLACYSLLVTVAFIIFWVKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 735 DNA PAT misc_feature 1..735 note CD19 scFv nucleotide source 1..735 mol_type other DNA organism synthetic construct gacatccagatgacacagaccacaagctccctgtctgccagcctgggcgacagagtgaccatctcctgtagggcctctcaggatatcagcaagtacctgaactggtatcagcagaagccagatggcacagtgaagctgctgatctaccacacctccaggctgcactctggagtgccaagccggttctccggatctggaagcggcaccgactattccctgacaatctctaacctggagcaggaggatatcgccacatacttttgccagcagggcaataccctgccatatacattcggcggaggaaccaagctggagatcaccggatccacatctggaagcggcaagccaggaagcggagagggatccacaaagggagaggtgaagctgcaggagagcggaccaggactggtggcaccatcccagtctctgagcgtgacctgtacagtgtccggcgtgtctctgcctgactacggcgtgtcctggatcaggcagccacctaggaagggactggagtggctgggcgtgatctggggctctgagaccacatactataattctgccctgaagagccgcctgaccatcatcaaggacaactccaagtctcaggtgtttctgaagatgaatagcctgcagaccgacgatacagccatctactattgcgccaagcactactattacggcggctcctacgccatggattattggggccagggcacctccgtgacagtgtctagc 245 AA PAT REGION 1..245 note CD19 scFv polypeptide source 1..245 mol_type protein organism synthetic construct DIQMTQTTSSLSASLGDRVTISCRASQDISKYLNWYQQKPDGTVKLLIYHTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQGNTLPYTFGGGTKLEITGSTSGSGKPGSGEGSTKGEVKLQESGPGLVAPSQSLSVTCTVSGVSLPDYGVSWIRQPPRKGLEWLGVIWGSETTYYNSALKSRLTIIKDNSKSQVFLKMNSLQTDDTAIYYCAKHYYYGGSYAMDYWGQGTSVTVSS 318 DNA PAT misc_feature 1..318 note CD19 scFv VL nucleotide source 1..318 mol_type other DNA organism synthetic construct gacatccagatgacacagaccacaagctccctgtctgccagcctgggcgacagagtgaccatctcctgtagggcctctcaggatatcagcaagtacctgaactggtatcagcagaagccagatggcacagtgaagctgctgatctaccacacctccaggctgcactctggagtgccaagccggttctccggatctggaagcggcaccgactattccctgacaatctctaacctggagcaggaggatatcgccacatacttttgccagcagggcaataccctgccatatacattcggcggaggaaccaagctggagatc 106 AA PAT REGION 1..106 note CD19 scFv VL polypeptide source 1..106 mol_type protein organism synthetic construct DIQMTQTTSSLSASLGDRVTISCRASQDISKYLNWYQQKPDGTVKLLIYHTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQGNTLPYTFGGGTKLEI 57 DNA PAT misc_feature 1..57 note CD19 scFv linker nucleotide source 1..57 mol_type other DNA organism synthetic construct accggatccacatctggaagcggcaagccaggaagcggagagggatccacaaaggga 19 AA PAT REGION 1..19 note CD19 scFv linker polypeptide source 1..19 mol_type protein organism synthetic construct TGSTSGSGKPGSGEGSTKG 360 DNA PAT misc_feature 1..360 note CD19 scFv VH nucleotide source 1..360 mol_type other DNA organism synthetic construct gaggtgaagctgcaggagagcggaccaggactggtggcaccatcccagtctctgagcgtgacctgtacagtgtccggcgtgtctctgcctgactacggcgtgtcctggatcaggcagccacctaggaagggactggagtggctgggcgtgatctggggctctgagaccacatactataattctgccctgaagagccgcctgaccatcatcaaggacaactccaagtctcaggtgtttctgaagatgaatagcctgcagaccgacgatacagccatctactattgcgccaagcactactattacggcggctcctacgccatggattattggggccagggcacctccgtgacagtgtctagc 360 DNA PAT misc_feature 1..360 note CD19 scFv VH polypeptide source 1..360 mol_type other DNA organism synthetic construct gaggtgaagctgcaggagagcggaccaggactggtggcaccatcccagtctctgagcgtgacctgtacagtgtccggcgtgtctctgcctgactacggcgtgtcctggatcaggcagccacctaggaagggactggagtggctgggcgtgatctggggctctgagaccacatactataattctgccctgaagagccgcctgaccatcatcaaggacaactccaagtctcaggtgtttctgaagatgaatagcctgcagaccgacgatacagccatctactattgcgccaagcactactattacggcggctcctacgccatggattattggggccagggcacctccgtgacagtgtctagc 39 DNA PAT misc_feature 1..39 note CD8 hinge nucleotide source 1..39 mol_type other DNA organism synthetic construct ggcgctgtgcacaccagaggactggatttcgcctgcgac 13 AA PAT REGION 1..13 note CD8 hinge polypeptide source 1..13 mol_type protein organism synthetic construct GAVHTRGLDFACD 81 DNA PAT misc_feature 1..81 note CD28 transmembrane domain nucleotide source 1..81 mol_type other DNA organism synthetic construct ttctgggtgctggtggtggtgggaggcgtgctggcctgttactccctgctggtgaccgtggcctttatcatcttctgggtg 27 AA PAT REGION 1..27 note CD28 transmembrane domain nucleotide source 1..27 mol_type protein organism synthetic construct FWVLVVVGGVLACYSLLVTVAFIIFWV 126 DNA PAT misc_feature 1..126 note 41BB nucleotide source 1..126 mol_type other DNA organism synthetic construct aagagaggcaggaagaagctgctgtatatctttaagcagcccttcatgcgccctgtgcagaccacacaggaggaggacggctgcagctgtcggtttccagaggaggaggagggaggatgcgagctg 336 DNA PAT misc_feature 1..336 note CD3zeta domain nucleotide source 1..336 mol_type other DNA organism synthetic construct cgcgtgaagttcagccggtccgccgatgcccctgcctaccagcagggccagaaccagctgtataacgagctgaatctgggccggagagaggagtacgacgtgctggataagaggaggggaagggacccagagatgggaggcaagcctcggagaaagaacccacaggagggcctgtacaatgagctgcagaaggacaagatggccgaggcctattctgagatcggcatgaagggagagaggcgccggggcaagggacacgatggcctgtaccagggcctgagcaccgccacaaaggacacatatgatgccctgcacatgcaggccctgccacctagg 1548 DNA PAT misc_feature 1..1548 note CD20 CAR nucleotide (with flag) source 1..1548 mol_type other DNA organism synthetic construct atgctgctgctggtgacaagcctgctgctgtgcgagctgcctcacccagcctttctgctgatccccgatatcgtgctgacccagtcccccgccatcctgtccgcctctcctggagagaaggtgaccatgacatgtcgggccagctcctctgtgaactacatggactggtatcagaagaagcctggcagctcccccaagccttggatctacgccacctccaatctggcctctggagtgccagcaagattcagcggatccggatctggcacaagctattccctgaccatctccagggtggaggcagaggatgcagcaacatactattgccagcagtggtctttcaacccccctacatttggcggcggcaccaagctggagatcaagggctctaccagcggaggaggaagcggaggaggatccggaggcggcggctctagcgaggtgcagctgcagcagtccggagcagagctggtgaagcctggagcctctgtgaagatgagctgtaaggcctccggctacaccttcacatcttataatatgcactgggtgaagcagacaccaggacagggactggagtggatcggagcaatctaccctggcaacggcgacaccagctataatcagaagtttaagggcaaggccaccctgacagccgataagtcctctagcacagcctacatgcagctgtcctctctgaccagcgaggactccgccgattactattgcgcccggtccaactactatggcagctcctattggttctttgacgtgtggggagcaggaacaaccgtgaccgtgtctagcgctgcaggaggcggaggatctggaggcggcggcggagactacaaagacgatgacgacaagttcgaagcaaagccaaccaccacacctgctcctagaccacctacacccgctcctaccatcgccagccagcctctgtctctgagacctgaggcctgtagacctgccgctggaggcgctgtgcacaccagaggactggatttcgcctgcgacatctacatctgggctcctctggctggaacatgcggcgtgctgctcctgagcctggtgatcacactgtactgcaagagaggcaggaagaagctgctgtatatctttaagcagcccttcatgcgccctgtgcagaccacacaggaggaggacggctgcagctgtcggtttccagaggaggaggagggaggatgcgagctgcgcgtgaagttcagccggtccgccgatgcccctgcctaccagcagggccagaaccagctgtataacgagctgaatctgggccggagagaggagtacgacgtgctggataagaggaggggaagggacccagagatgggaggcaagcctcggagaaagaacccacaggagggcctgtacaatgagctgcagaaggacaagatggccgaggcctattctgagatcggcatgaagggagagaggcgccggggcaagggacacgatggcctgtaccagggcctgagcaccgccacaaaggacacctatgatgccctgcacatgcaggccctgccccctcgg 516 AA PAT REGION 1..516 note CD20 CAR polypeptide (with flag sequence) source 1..516 mol_type protein organism synthetic construct MLLLVTSLLLCELPHPAFLLIPDIVLTQSPAILSASPGEKVTMTCRASSSVNYMDWYQKKPGSSPKPWIYATSNLASGVPARFSGSGSGTSYSLTISRVEAEDAATYYCQQWSFNPPTFGGGTKLEIKGSTSGGGSGGGSGGGGSSEVQLQQSGAELVKPGASVKMSCKASGYTFTSYNMHWVKQTPGQGLEWIGAIYPGNGDTSYNQKFKGKATLTADKSSSTAYMQLSSLTSEDSADYYCARSNYYGSSYWFFDVWGAGTTVTVSSAAGGGGSGGGGGDYKDDDDKFEAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 738 DNA PAT misc_feature 1..738 note CD20 scFv nucleotide source 1..738 mol_type other DNA organism synthetic construct gatatcgtgctgacccagtcccccgccatcctgtccgcctctcctggagagaaggtgaccatgacatgtcgggccagctcctctgtgaactacatggactggtatcagaagaagcctggcagctcccccaagccttggatctacgccacctccaatctggcctctggagtgccagcaagattcagcggatccggatctggcacaagctattccctgaccatctccagggtggaggcagaggatgcagcaacatactattgccagcagtggtctttcaacccccctacatttggcggcggcaccaagctggagatcaagggctctaccagcggaggaggaagcggaggaggatccggaggcggcggctctagcgaggtgcagctgcagcagtccggagcagagctggtgaagcctggagcctctgtgaagatgagctgtaaggcctccggctacaccttcacatcttataatatgcactgggtgaagcagacaccaggacagggactggagtggatcggagcaatctaccctggcaacggcgacaccagctataatcagaagtttaagggcaaggccaccctgacagccgataagtcctctagcacagcctacatgcagctgtcctctctgaccagcgaggactccgccgattactattgcgcccggtccaactactatggcagctcctattggttctttgacgtgtggggagcaggaacaaccgtgaccgtgtctagc 246 AA PAT REGION 1..246 note CD20 scFv polypeptide source 1..246 mol_type protein organism synthetic construct DIVLTQSPAILSASPGEKVTMTCRASSSVNYMDWYQKKPGSSPKPWIYATSNLASGVPARFSGSGSGTSYSLTISRVEAEDAATYYCQQWSFNPPTFGGGTKLEIKGSTSGGGSGGGSGGGGSSEVQLQQSGAELVKPGASVKMSCKASGYTFTSYNMHWVKQTPGQGLEWIGAIYPGNGDTSYNQKFKGKATLTADKSSSTAYMQLSSLTSEDSADYYCARSNYYGSSYWFFDVWGAGTTVTVSS 318 DNA PAT misc_feature 1..318 note CD20 scFv VL nucleotide source 1..318 mol_type other DNA organism synthetic construct gatatcgtgctgacccagtcccccgccatcctgtccgcctctcctggagagaaggtgaccatgacatgtcgggccagctcctctgtgaactacatggactggtatcagaagaagcctggcagctcccccaagccttggatctacgccacctccaatctggcctctggagtgccagcaagattcagcggatccggatctggcacaagctattccctgaccatctccagggtggaggcagaggatgcagcaacatactattgccagcagtggtctttcaacccccctacatttggcggcggcaccaagctggagatcaag 106 AA PAT REGION 1..106 note CD20 scFv VL polypeptide source 1..106 mol_type protein organism synthetic construct DIVLTQSPAILSASPGEKVTMTCRASSSVNYMDWYQKKPGSSPKPWIYATSNLASGVPARFSGSGSGTSYSLTISRVEAEDAATYYCQQWSFNPPTFGGGTKLEIK 54 DNA PAT misc_feature 1..54 note 218 linker nucleotide source 1..54 mol_type other DNA organism synthetic construct ggctctaccagcggaggaggaagcggaggaggatccggaggcggcggctctagc 18 AA PAT REGION 1..18 note 218 linker polypeptide source 1..18 mol_type protein organism synthetic construct GSTSGGGSGGGSGGGGSS 366 DNA PAT misc_feature 1..366 note CD20 scFv VH nucleotide source 1..366 mol_type other DNA organism synthetic construct gaggtgcagctgcagcagtccggagcagagctggtgaagcctggagcctctgtgaagatgagctgtaaggcctccggctacaccttcacatcttataatatgcactgggtgaagcagacaccaggacagggactggagtggatcggagcaatctaccctggcaacggcgacaccagctataatcagaagtttaagggcaaggccaccctgacagccgataagtcctctagcacagcctacatgcagctgtcctctctgaccagcgaggactccgccgattactattgcgcccggtccaactactatggcagctcctattggttctttgacgtgtggggagcaggaacaaccgtgaccgtgtctagc 122 AA PAT REGION 1..122 note CD20 scFv VH polypeptide source 1..122 mol_type protein organism synthetic construct EVQLQQSGAELVKPGASVKMSCKASGYTFTSYNMHWVKQTPGQGLEWIGAIYPGNGDTSYNQKFKGKATLTADKSSSTAYMQLSSLTSEDSADYYCARSNYYGSSYWFFDVWGAGTTVTVSS 24 DNA PAT misc_feature 1..24 note Flag nucleotide source 1..24 mol_type other DNA organism synthetic construct gactacaaagacgatgacgacaag 8 AA PAT REGION 1..8 note Flag polypeptide source 1..8 mol_type protein organism synthetic construct DYKDDDDK 216 DNA PAT misc_feature 1..216 note CD8 HTM nucleotide source 1..216 mol_type other DNA organism synthetic construct gcaaagccaaccaccacacctgctcctagaccacctacacccgctcctaccatcgccagccagcctctgtctctgagacctgaggcctgtagacctgccgctggaggcgctgtgcacaccagaggactggatttcgcctgcgacatctacatctgggctcctctggctggaacatgcggcgtgctgctcctgagcctggtgatcacactgtactgc 72 AA PAT REGION 1..72 note CD8 HTM polypeptide source 1..72 mol_type protein organism synthetic construct AKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYC 336 DNA PAT misc_feature 1..336 note CD3zeta nucleotide source 1..336 mol_type other DNA organism synthetic construct cgcgtgaagttcagccggtccgccgatgcccctgcctaccagcagggccagaaccagctgtataacgagctgaatctgggccggagagaggagtacgacgtgctggataagaggaggggaagggacccagagatgggaggcaagcctcggagaaagaacccacaggagggcctgtacaatgagctgcagaaggacaagatggccgaggcctattctgagatcggcatgaagggagagaggcgccggggcaagggacacgatggcctgtaccagggcctgagcaccgccacaaaggacacctatgatgccctgcacatgcaggccctgccccctcgg 1497 DNA PAT misc_feature 1..1497 note TagCAR nucleotide source 1..1497 mol_type other DNA organism synthetic construct ctgctgctggtgacaagcctgctgctgtgcgagctgcctcacccagcctttctgctgatcccctccgtgctgacccagcctagctccgtgtctgccgcaccaggacagaaggtgacaatcagctgttccggctctaccagcaacatcggcaacaattacgtgagctggtaccagcagcaccctggcaaggccccaaagctgatgatctacgacgtgtccaagaggccatctggagtgcctgatcggttctccggctctaagagcggcaattccgcctctctggacatcagcggactgcagtccgaggacgaggcagattactattgcgccgcctgggacgatagcctgtccgagtttctgttcggcaccggcacaaagctgaccgtgctgggctctacaagcggatccggcaagccaggatctggagagggcagcacaaagggacaggtgcagctggtggagagcggaggaaacctggtgcagccaggaggctccctgcgcctgtcttgtgccgccagcggctttaccttcggctcttttagcatgtcctgggtgcgccaggcacctggaggaggactggagtgggtggccggcctgagcgcccggtctagcctgacacactatgccgactccgtgaagggccgcttcaccatctcccgggataacgccaagaatagcgtgtacctgcagatgaatagcctgcgggtggaggacacagccgtgtactattgcgccaggcgctcctatgattcctctggctactggggccacttttactcttatatggacgtgtggggacagggcaccctggtgacagtgagctccaccaccacacctgctcctagaccacctacacccgctcctaccatcgccagccagcctctgtctctgagacctgaggcctgtagacctgccgctggaggcgctgtgcacaccagaggactggatttcgcctgcgacatctacatctgggctcctctggctggaacatgcggcgtgctgctcctgagcctggtgatcacactgtactgcaagcgcggccggaagaagctgctctacatctttaagcagccattcatgcgccccgtgcagaccacacaggaggaggacggctgctcctgtcggtttccagaggaggaggagggaggatgtgagctgagagtgaagttctctaggagcgccgatgcccctgcctatcagcagggacagaaccagctgtacaacgagctgaatctgggccggagagaggagtacgacgtgctggataagaggaggggaagagacccagagatgggaggcaagcctcggagaaagaacccacaggagggcctgtataatgagctgcagaaggacaagatggccgaggcctactccgagatcggcatgaagggagagaggcgccggggcaagggacacgatggcctgtatcagggcctgagcaccgccacaaaggacacatacgatgccctgcacatgcaggccctgcctccaaggtga 1497 DNA PAT misc_feature 1..1497 note TagCAR nucleotide source 1..1497 mol_type other DNA organism synthetic construct atgctgctgctggtgacaagcctgctgctgtgcgagctgcctcacccagcctttctgctgatcccctccgtgctgacccagcctagctccgtgtctgccgcaccaggacagaaggtgacaatcagctgttccggctctaccagcaacatcggcaacaattacgtgagctggtaccagcagcaccctggcaaggccccaaagctgatgatctacgacgtgtccaagaggccatctggagtgcctgatcggttctccggctctaagagcggcaattccgcctctctggacatcagcggactgcagtccgaggacgaggcagattactattgcgccgcctgggacgatagcctgtccgagtttctgttcggcaccggcacaaagctgaccgtgctgggctctacaagcggatccggcaagccaggatctggagagggcagcacaaagggacaggtgcagctggtggagagcggaggaaacctggtgcagccaggaggctccctgcgcctgtcttgtgccgccagcggctttaccttcggctcttttagcatgtcctgggtgcgccaggcacctggaggaggactggagtgggtggccggcctgagcgcccggtctagcctgacacactatgccgactccgtgaagggccgcttcaccatctcccgggataacgccaagaatagcgtgtacctgcagatgaatagcctgcgggtggaggacacagccgtgtactattgcgccaggcgctcctatgattcctctggctactggggccacttttactcttatatggacgtgtggggacagggcaccctggtgacagtgagctccaccaccacacctgctcctagaccacctacacccgctcctaccatcgccagccagcctctgtctctgagacctgaggcctgtagacctgccgctggaggcgctgtgcacaccagaggactggatttcgcctgcgacatctacatctgggctcctctggctggaacatgcggcgtgctgctcctgagcctggtgatcacactgtactgcaagcgcggccggaagaagctgctctacatctttaagcagccattcatgcgccccgtgcagaccacacaggaggaggacggctgctcctgtcggtttccagaggaggaggagggaggatgtgagctgagagtgaagttctctaggagcgccgatgcccctgcctatcagcagggacagaaccagctgtacaacgagctgaatctgggccggagagaggagtacgacgtgctggataagaggaggggaagagacccagagatgggaggcaagcctcggagaaagaacccacaggagggcctgtataatgagctgcagaaggacaagatggccgaggcctactccgagatcggcatgaagggagagaggcgccggggcaagggacacgatggcctgtatcagggcctgagcaccgccacaaaggacacatacgatgccctgcacatgcaggccctgcctccaagg 498 AA PAT REGION 1..498 note TagCAR polypeptide source 1..498 mol_type protein organism synthetic construct LLLVTSLLLCELPHPAFLLIPSVLTQPSSVSAAPGQKVTISCSGSTSNIGNNYVSWYQQHPGKAPKLMIYDVSKRPSGVPDRFSGSKSGNSASLDISGLQSEDEADYYCAAWDDSLSEFLFGTGTKLTVLGSTSGSGKPGSGEGSTKGQVQLVESGGNLVQPGGSLRLSCAASGFTFGSFSMSWVRQAPGGGLEWVAGLSARSSLTHYADSVKGRFTISRDNAKNSVYLQMNSLRVEDTAVYYCARRSYDSSGYWGHFYSYMDVWGQGTLVTVSSTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 499 AA PAT source 1..499 mol_type protein organism synthetic construct UNSURE 1..499 note TagCAR polypeptidee MLLLVTSLLLCELPHPAFLLIPSVLTQPSSVSAAPGQKVTISCSGSTSNIGNNYVSWYQQHPGKAPKLMIYDVSKRPSGVPDRFSGSKSGNSASLDISGLQSEDEADYYCAAWDDSLSEFLFGTGTKLTVLGSTSGSGKPGSGEGSTKGQVQLVESGGNLVQPGGSLRLSCAASGFTFGSFSMSWVRQAPGGGLEWVAGLSARSSLTHYADSVKGRFTISRDNAKNSVYLQMNSLRVEDTAVYYCARRSYDSSGYWGHFYSYMDVWGQGTLVTVSSTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR 762 DNA PAT misc_feature 1..762 note E2 scFv nucleotide source 1..762 mol_type other DNA organism synthetic construct tccgtgctgacccagcctagctccgtgtctgccgcaccaggacagaaggtgacaatcagctgttccggctctaccagcaacatcggcaacaattacgtgagctggtaccagcagcaccctggcaaggccccaaagctgatgatctacgacgtgtccaagaggccatctggagtgcctgatcggttctccggctctaagagcggcaattccgcctctctggacatcagcggactgcagtccgaggacgaggcagattactattgcgccgcctgggacgatagcctgtccgagtttctgttcggcaccggcacaaagctgaccgtgctgggctctacaagcggatccggcaagccaggatctggagagggcagcacaaagggacaggtgcagctggtggagagcggaggaaacctggtgcagccaggaggctccctgcgcctgtcttgtgccgccagcggctttaccttcggctcttttagcatgtcctgggtgcgccaggcacctggaggaggactggagtgggtggccggcctgagcgcccggtctagcctgacacactatgccgactccgtgaagggccgcttcaccatctcccgggataacgccaagaatagcgtgtacctgcagatgaatagcctgcgggtggaggacacagccgtgtactattgcgccaggcgctcctatgattcctctggctactggggccacttttactcttatatggacgtgtggggacagggcaccctggtgacagtgagctcc 254 AA PAT REGION 1..254 note E2 scFv polypeptide source 1..254 mol_type protein organism synthetic construct SVLTQPSSVSAAPGQKVTISCSGSTSNIGNNYVSWYQQHPGKAPKLMIYDVSKRPSGVPDRFSGSKSGNSASLDISGLQSEDEADYYCAAWDDSLSEFLFGTGTKLTVLGSTSGSGKPGSGEGSTKGQVQLVESGGNLVQPGGSLRLSCAASGFTFGSFSMSWVRQAPGGGLEWVAGLSARSSLTHYADSVKGRFTISRDNAKNSVYLQMNSLRVEDTAVYYCARRSYDSSGYWGHFYSYMDVWGQGTLVTVSS 327 DNA PAT misc_feature 1..327 note E2 scFv VL nucleotide source 1..327 mol_type other DNA organism synthetic construct tccgtgctgacccagcctagctccgtgtctgccgcaccaggacagaaggtgacaatcagctgttccggctctaccagcaacatcggcaacaattacgtgagctggtaccagcagcaccctggcaaggccccaaagctgatgatctacgacgtgtccaagaggccatctggagtgcctgatcggttctccggctctaagagcggcaattccgcctctctggacatcagcggactgcagtccgaggacgaggcagattactattgcgccgcctgggacgatagcctgtccgagtttctgttcggcaccggcacaaagctgaccgtgctg 109 AA PAT REGION 1..109 note E2 scFv VL polypeptide source 1..109 mol_type protein organism synthetic construct SVLTQPSSVSAAPGQKVTISCSGSTSNIGNNYVSWYQQHPGKAPKLMIYDVSKRPSGVPDRFSGSKSGNSASLDISGLQSEDEADYYCAAWDDSLSEFLFGTGTKLTVL 54 DNA PAT misc_feature 1..54 note E2 scFv linker nucleotide source 1..54 mol_type other DNA organism synthetic construct ggctctacaagcggatccggcaagccaggatctggagagggcagcacaaaggga 18 AA PAT REGION 1..18 note E2 scFv linker polypeptide source 1..18 mol_type protein organism synthetic construct GSTSGSGKPGSGEGSTKG 381 DNA PAT misc_feature 1..381 note E2 scFv VH nucleotide source 1..381 mol_type other DNA organism synthetic construct caggtgcagctggtggagagcggaggaaacctggtgcagccaggaggctccctgcgcctgtcttgtgccgccagcggctttaccttcggctcttttagcatgtcctgggtgcgccaggcacctggaggaggactggagtgggtggccggcctgagcgcccggtctagcctgacacactatgccgactccgtgaagggccgcttcaccatctcccgggataacgccaagaatagcgtgtacctgcagatgaatagcctgcgggtggaggacacagccgtgtactattgcgccaggcgctcctatgattcctctggctactggggccacttttactcttatatggacgtgtggggacagggcaccctggtgacagtgagctcc 127 AA PAT REGION 1..127 note E2 scFv VH polypeptide source 1..127 mol_type protein organism synthetic construct QVQLVESGGNLVQPGGSLRLSCAASGFTFGSFSMSWVRQAPGGGLEWVAGLSARSSLTHYADSVKGRFTISRDNAKNSVYLQMNSLRVEDTAVYYCARRSYDSSGYWGHFYSYMDVWGQGTLVTVSS 207 DNA PAT misc_feature 1..207 note CD8 HTM nucleotide source 1..207 mol_type other DNA organism synthetic construct accaccacacctgctcctagaccacctacacccgctcctaccatcgccagccagcctctgtctctgagacctgaggcctgtagacctgccgctggaggcgctgtgcacaccagaggactggatttcgcctgcgacatctacatctgggctcctctggctggaacatgcggcgtgctgctcctgagcctggtgatcacactgtactgc 69 AA PAT REGION 1..69 note CD8 HTM polypeptide source 1..69 mol_type protein organism synthetic construct TTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYC 141 DNA PAT misc_feature 1..141 note CD8 Hinge nucleotide source 1..141 mol_type other DNA organism synthetic construct accaccacacctgctcctagaccacctacacccgctcctaccatcgccagccagcctctgtctctgagacctgaggcctgtagacctgccgctggaggcgctgtgcacaccagaggactggatttcgcctgcgacatctac 47 AA PAT REGION 1..47 note CD8 Hinge polypeptide source 1..47 mol_type protein organism synthetic construct TTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIY 66 DNA PAT misc_feature 1..66 note CD8 transmembrane nucleotide source 1..66 mol_type other DNA organism synthetic construct atctgggctcctctggctggaacatgcggcgtgctgctcctgagcctggtgatcacactgtactgc 22 AA PAT REGION 1..22 note CD8 transmembrane polypeptide source 1..22 mol_type protein organism synthetic construct IWAPLAGTCGVLLLSLVITLYC 248 AA PAT REGION 1..248 note Anti-CD3 scFv (VL-G4S x 3 linker - VH) source 1..248 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCSASSSVSYMNWYQQTPGKAPKRWIYDTSKLASGVPSRFSGSGSGTDYTFTISSLQPEDIATYYCQQWSSNPFTFGQGTKLQITRTSGGGGSGGGGSGGGGSQVQLVQSGGGVVQPGRSLRLSCKASGYTFTRYTMHWVRQAPGKGLEWIGYINPSRGYTNYNQKVKDRFTISRDNSKNTAFLQMDSLRPEDTGVYFCARYYDDHYCLDYWGQGTPVTVSSAAAKP 744 DNA PAT misc_feature 1..744 note Anti-CD3 scFv (VL-G4S x 3 linker - VH) source 1..744 mol_type other DNA organism synthetic construct gatatccagatgacccagtccccaagctccctgagcgcctccgtgggcgaccgggtgacaatcacctgcagcgcctctagctccgtgtcctacatgaactggtatcagcagacacctggcaaggccccaaagagatggatctacgataccagcaagctggcctccggcgtgccttctaggttttctggcagcggctccggcacagattatacattcaccatctctagcctgcagccagaggacatcgccacctactattgccagcagtggtcctctaatccctttacattcggccagggcaccaagctgcagatcacaagaacctctggaggaggaggaagcggaggaggaggatccggcggcggcggctctcaggtgcagctggtgcagagcggaggaggagtggtgcagccaggcagaagcctgaggctgtcctgtaaggcctctggctacacattcaccagatatacaatgcactgggtgaggcaggcaccaggcaagggactggagtggatcggctacatcaacccctccaggggctacaccaactataatcagaaggtgaaggatcggttcaccatcagcagggacaactccaagaataccgccttcctgcagatggacagcctgaggccagaggataccggcgtgtacttttgcgcccggtactatgacgatcactactgtctggattattggggccagggaacaccagtgaccgtgagctccgccgcagcaaagcct 245 AA PAT REGION 1..245 note Anti-CD3 scFv VL-G4S x 3 linker-VH source 1..245 mol_type protein organism synthetic construct DIQMTQSPSSLSASVGDRVTITCSASSSVSYMNWYQQTPGKAPKRWIYDTSKLASGVPSRFSGSGSGTDYTFTISSLQPEDIATYYCQQWSSNPFTFGQGTKLQITRTSGGGGSGGGGSGGGGSQVQLVQSGGGVVQPGRSLRLSCKASGYTFTRYTMHWVRQAPGKGLEWIGYINPSRGYTNYNQKVKDRFTISRDNSKNTAFLQMDSLRPEDTGVYFCARYYDDHYCLDYWGQGTPVTVSSAS 735 DNA PAT misc_feature 1..735 note Anti-CD3 scFv VL-G4S x 3 linker-VH source 1..735 mol_type other DNA organism synthetic construct gacatccagatgacccagtctcctagcagcctcagcgctagcgtgggcgatagagtgaccatcacatgtagcgccagcagcagcgtgtcctacatgaactggtaccagcaaacacctggaaaggcccctaaaaggtggatctatgacacatctaagctggcttctggagtgccatctagattttctggcagcggctccggcactgattatacattcaccatcagcagcctgcagcccgaggatatcgccacctactactgtcagcagtggtcctctaatcccttcaccttcggccagggcaccaagctgcagatcaccagaaccagcggcgggggaggaagcggcgggggaggatctggcggcggcggcagccaggtgcagctggtgcagagcggcggcggcgtggtgcaacctggcagaagcctgagactgagctgcaaggcctctggctacaccttcacccggtacaccatgcattgggtgcggcaggcccctggcaagggcctggaatggattggatacatcaaccccagcagaggctacaccaactacaaccagaaggtgaaggacagattcacaatttctcgggacaacagcaagaataccgccttcctgcaaatggactccctgcgcccagaagataccggcgtgtacttctgcgctagatattacgacgaccactactgcctggactactggggccagggcacccctgtgaccgtgtccagcgcctcc 511 AA PAT REGION 1..511 note Cocal envelope polypeptide source 1..511 mol_type protein organism synthetic construct NFLLLTFIVLPLCSHAKFSIVFPQSQKGNWKNVPSSYHYCPSSSDQNWHNDLLGITMKVKMPKTHKAIQADGWMCHAAKWITTCDFRWYGPKYITHSIHSIQPTSEQCKESIKQTKQGTWMSPGFPPQNCGYATVTDSVAVVVQATPHHVLVDEYTGEWIDSQFPNGKCETEECETVHNSTVWYSDYKVTGLCDATLVDTEITFFSEDGKKESIGKPNTGYRSNYFAYEKGDKVCKMNYCKHAGVRLPSGVWFEFVDQDVYAAAKLPECPVGATISAPTQTSVDVSLILDVERILDYSLCQETWSKIRSKQPVSPVDLSYLAPKNPGTGPAFTIINGTLKYFETRYIRIDIDNPIISKMVGKISGSQTERELWTEWFPYEGVEIGPNGILKTPTGYKFPLFMIGHGMLDSDLHKTSQAEVFEHPHLAEAPKQLPEEETLFFGDTGISKNPVELIEGWFSSWKSTVVTFFFAIGVFILLYVVARIVIAVRYRYQGSNNKRIYNDIEMSRFRK 1539 DNA PAT misc_feature 1..1539 note Cocal envelope nucleotide source 1..1539 mol_type other DNA organism synthetic construct atgaactttctgctgctgaccttcatcgtgctgcctctgtgcagccacgccaagttttccatcgtgttcccacagtcccagaagggcaactggaagaatgtgcccagctcctaccactattgtccttctagctccgaccagaactggcacaatgatctgctgggcatcaccatgaaggtgaagatgcctaagacacacaaggccatccaggcagatggatggatgtgccacgcagccaagtggatcaccacatgtgactttcggtggtacggccccaagtatatcacccacagcatccactccatccagcctacaagcgagcagtgcaaggagtccatcaagcagaccaagcagggcacatggatgtctcccggcttcccccctcagaactgtggctacgccaccgtgacagatagcgtggcagtggtggtgcaggcaaccccacaccacgtgctggtggatgagtatacaggcgagtggatcgacagccagtttcccaacggcaagtgcgagaccgaggagtgtgagacagtgcacaattctaccgtgtggtacagcgattataaggtgaccggcctgtgcgacgccacactggtggataccgagatcacattcttttccgaggacggcaagaaggagtctatcggcaagcccaacaccggctacaggtctaattacttcgcctatgagaagggcgataaggtgtgcaagatgaattattgtaagcacgccggggtgcggctgccaagcggcgtgtggtttgagttcgtggaccaggacgtgtacgcagcagcaaagctgccagagtgcccagtgggagcaaccatcagcgcccccacccagacatctgtggacgtgagcctgatcctggatgtggagagaatcctggactactccctgtgccaggagacatggtccaagatccgctctaagcagcccgtgagcccagtggacctgtcttacctggcaccaaagaaccctggaacaggacctgcctttaccatcatcaatggcacactgaagtacttcgagacccggtatatcagaatcgacatcgataacccaatcatctccaagatggtgggcaagatctccggctctcagaccgagagagagctgtggacagagtggttcccatacgagggcgtggagatcggccccaatggcatcctgaagacccctacaggctataagtttccactgttcatgatcggccacggcatgctggactctgatctgcacaagaccagccaggccgaggtgtttgagcacccacacctggcagaggcaccaaagcagctgcccgaggaggagaccctgttctttggcgatacaggcatctccaagaaccctgtggagctgatcgagggctggttttctagctggaagtctaccgtggtgacattctttttcgccatcggcgtgttcatcctgctgtacgtggtggcaaggatcgtgatcgccgtgcggtacagatatcagggcagcaacaataagagaatctataatgacatcgagatgtccaggttccgcaagtga 1533 DNA PAT misc_feature 1..1533 note Cocal envelope nucleotide source 1..1533 mol_type other DNA organism synthetic construct aattttctgctgctgaccttcatcgtgctgcctctgtgcagccacgccaagttttccatcgtgttcccacagtcccagaagggcaactggaagaatgtgccctctagctaccactattgcccttcctctagcgaccagaactggcacaatgatctgctgggcatcacaatgaaggtgaagatgcccaagacccacaaggccatccaggcagatggatggatgtgccacgcagccaagtggatcacaacctgtgactttcggtggtacggccccaagtatatcacacactccatccactctatccagcctacctccgagcagtgcaaggagtctatcaagcagacaaagcagggcacctggatgagccctggcttcccaccccagaactgtggctacgccacagtgaccgactccgtggcagtggtggtgcaggcaacacctcaccacgtgctggtggatgagtataccggcgagtggatcgacagccagtttccaaacggcaagtgcgagacagaggagtgtgagaccgtgcacaattctacagtgtggtacagcgattataaggtgacaggcctgtgcgacgccaccctggtggatacagagatcaccttcttttctgaggacggcaagaaggagagcatcggcaagcccaacaccggctacagatccaattacttcgcctatgagaagggcgataaggtgtgcaagatgaattattgtaagcacgccggggtgcggctgcctagcggcgtgtggtttgagttcgtggaccaggacgtgtacgcagcagcaaagctgcctgagtgcccagtgggagcaaccatctccgccccaacacagacctccgtggacgtgtctctgatcctggatgtggagcgcatcctggactacagcctgtgccaggagacctggagcaagatccggtccaagcagcccgtgtcccctgtggacctgtcttacctggcaccaaagaacccaggaaccggaccagcctttacaatcatcaatggcaccctgaagtacttcgagacccgctatatccggatcgacatcgataaccctatcatcagcaagatggtgggcaagatctctggcagccagacagagagagagctgtggaccgagtggttcccttacgagggcgtggagatcggcccaaatggcatcctgaagacaccaaccggctataagtttcccctgttcatgatcggccacggcatgctggacagcgatctgcacaagacctcccaggccgaggtgtttgagcacccacacctggcagaggcaccaaagcagctgcctgaggaggagacactgttctttggcgataccggcatctctaagaaccccgtggagctgatcgagggctggttttcctcttggaagagcacagtggtgaccttctttttcgccatcggcgtgttcatcctgctgtacgtggtggccagaatcgtgatcgccgtgagatacaggtatcagggctccaacaataagaggatctataatgacatcgagatgtctcgcttccggaag 116 AA PAT REGION 1..116 note Rev polypeptide source 1..116 mol_type protein organism synthetic construct MAGRSGDSDEDLLKAVRLIKFLYQSNPPPNPEGTRQARRNRRRRWRERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILVESPTILESGAKE 351 DNA PAT misc_feature 1..351 note Rev nucleotide source 1..351 mol_type other DNA organism synthetic construct atggccggcagaagcggcgacagcgacgaggatctgctgaaagccgtgcggctgatcaagttcctgtaccagagcaaccctcctcctaaccccgagggcaccagacaggctagacggaaccgcagaagaaggtggcgggaacggcaaagacagatccactctatcagcgagagaatcctgagcacctacctgggaagatccgccgagcctgtccccctgcagctgcctccactggaaagactgaccctggattgtaatgaggactgcggcacaagcggaacccagggcgtgggcagcccccagattctggtggaatcccctacaatcctcgagtctggcgccaaggaatga 1482 DNA PAT source 1..1482 mol_type other DNA organism synthetic construct unsure 1..1482 note Anti-CD20 CAR (without flag) nucleotide atgctgctgctggtgacaagcctgctgctgtgcgagctgcctcacccagcctttctgctgatccccgatatcgtgctgacccagtcccccgccatcctgtccgcctctcctggagagaaggtgaccatgacatgtcgggccagctcctctgtgaactacatggactggtatcagaagaagcctggcagctcccccaagccttggatctacgccacctccaatctggcctctggagtgccagcaagattcagcggatccggatctggcacaagctattccctgaccatctccagggtggaggcagaggatgcagcaacatactattgccagcagtggtctttcaacccccctacatttggcggcggcaccaagctggagatcaagggctctaccagcggaggaggaagcggaggaggatccggaggcggcggctctagcgaggtgcagctgcagcagtccggagcagagctggtgaagcctggagcctctgtgaagatgagctgtaaggcctccggctacaccttcacatcttataatatgcactgggtgaagcagacaccaggacagggactggagtggatcggagcaatctaccctggcaacggcgacaccagctataatcagaagtttaagggcaaggccaccctgacagccgataagtcctctagcacagcctacatgcagctgtcctctctgaccagcgaggactccgccgattactattgcgcccggtccaactactatggcagctcctattggttctttgacgtgtggggagcaggaacaaccgtgaccgtgtctagcgcaaagccaaccaccacacctgctcctagaccacctacacccgctcctaccatcgccagccagcctctgtctctgagacctgaggcctgtagacctgccgctggaggcgctgtgcacaccagaggactggatttcgcctgcgacatctacatctgggctcctctggctggaacatgcggcgtgctgctcctgagcctggtgatcacactgtactgcaagagaggcaggaagaagctgctgtatatctttaagcagcccttcatgcgccctgtgcagaccacacaggaggaggacggctgcagctgtcggtttccagaggaggaggagggaggatgcgagctgcgcgtgaagttcagccggtccgccgatgcccctgcctaccagcagggccagaaccagctgtataacgagctgaatctgggccggagagaggagtacgacgtgctggataagaggaggggaagggacccagagatgggaggcaagcctcggagaaagaacccacaggagggcctgtacaatgagctgcagaaggacaagatggccgaggcctattctgagatcggcatgaagggagagaggcgccggggcaagggacacgatggcctgtaccagggcctgagcaccgccacaaaggacacctatgatgccctgcacatgcaggccctgccccctcgg 494 AA PAT source 1..494 mol_type protein organism synthetic construct UNSURE 1..494 note Anti-CD20 CAR (without flag) polypeptide MLLLVTSLLLCELPHPAFLLIPDIVLTQSPAILSASPGEKVTMTCRASSSVNYMDWYQKKPGSSPKPWIYATSNLASGVPARFSGSGSGTSYSLTISRVEAEDAATYYCQQWSFNPPTFGGGTKLEIKGSTSGGGSGGGSGGGGSSEVQLQQSGAELVKPGASVKMSCKASGYTFTSYNMHWVKQTPGQGLEWIGAIYPGNGDTSYNQKFKGKATLTADKSSSTAYMQLSSLTSEDSADYYCARSNYYGSSYWFFDVWGAGTTVTVSSAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR