SG 11201905250W 2017-12-11 SP101212SGA US US 62/432,879 2016-12-12 The Regents of the University of Colorado USE OF BIOLOGICAL RNA SCAFFOLDS WITH IN VITRO SELECTION TO GENERATE ROBUST SMALL MOLECULE BINDING APTAMERS FOR GENETICALLY ENCODABLE BIOSENSORS 118 149 DNA PAT misc_feature 1..149 note GR/SSIII library template misc_feature 56..62 note n is a, c, g, or t misc_feature 82..91 note n is a, c, g, or t misc_feature 109..114 note n is a, c, g, or t source 1..149 mol_type other DNA organism synthetic construct gcgcgcgaattctaatacgactcactataggacttcggtccaagctaatgcactcnnnnnnncgcgtggatatggcacgcannnnnnnnnngggcaccgtaaatgtccnnnnnngggtgcattagcaaaatcgggcttcggtccggttc 147 DNA PAT misc_feature 1..147 note GR/GsI library template misc_feature 55..61 note n is a, c, g, or t misc_feature 81..90 note n is a, c, g, or t misc_feature 108..113 note n is a, c, g, or t source 1..147 mol_type other DNA organism synthetic construct gcgcgcgaattctaatacgactcactataggacttcggtccttggataggactcnnnnnnncgcgtggatatggcacgcannnnnnnnnngggcaccgtaaatgtccnnnnnngggtcctatccccaatcgggcttcggtccggttc 164 DNA PAT misc_feature 1..164 note CDG/GsI library template misc_feature 54..62 note n is a, c, g, or t misc_feature 87..91 note n is a, c, g, or t misc_feature 125..131 note n is a, c, g, or t source 1..164 mol_type other DNA organism synthetic construct gcgcgcgaattctaatacgactcactataggacttcggtccttggataggacannnnnnnnncaaaccattcgaaagagtgggacgnnnnncctccggcctaaaccgaaaggtaggtagcggggnnnnnnntgtcctatccccaatcgggcttcggtccggttc 143 DNA PAT misc_feature 1..143 note HH/GsI library template misc_feature 54..57 note n is a, c, g, or t misc_feature 84..93 note n is a, c, g, or t misc_feature 104..110 note n is a, c, g, or t source 1..143 mol_type other DNA organism synthetic construct gcgcgcgaattctaatacgactcactataggacttcggtccttggataggagcnnnntggtatccaatgaaaatgtactaccannnnnnnnnncccaaataggnnnnnnngctcctatccccaatcgggcttcggtccggttc 55 DNA PAT misc_feature 1..55 note T7 site appending primer source 1..55 mol_type other DNA organism synthetic construct gcgcgcgaattctaatacgactcactataggacttcggtccaagctaatgcactc 18 DNA PAT misc_feature 1..18 note RT-PCR primer source 1..18 mol_type other DNA organism synthetic construct gaaccggaccgaagcccg 66 DNA PAT misc_feature 1..66 note HTS reverse sequencing primer, GR/SSIII source 1..66 mol_type other DNA organism synthetic construct caagcagaagacggcatacgagatgtcgtgtagcctagtcagtcagccgaaccggaccgaagcccg 66 DNA PAT misc_feature 1..66 note HTS reverse sequencing primer, GR/GsI source 1..66 mol_type other DNA organism synthetic construct caagcagaagacggcatacgagattggtcaacgataagtcagtcagccgaaccggaccgaagcccg 66 DNA PAT misc_feature 1..66 note HTS reverse sequencing primer, CDG/GsI source 1..66 mol_type other DNA organism synthetic construct caagcagaagacggcatacgagatatcaccaggtgtagtcagtcagccgaaccggaccgaagcccg 66 DNA PAT misc_feature 1..66 note HTS reverse sequencing primer, HH/GsI source 1..66 mol_type other DNA organism synthetic construct caagcagaagacggcatacgagatgctgtacggattagtcagtcagccgaaccggaccgaagcccg 71 DNA PAT misc_feature 1..71 note Forward sequencing primer source 1..71 mol_type other DNA organism synthetic construct aatgatacggcgaccaccgagatctacactatggtaattgtgcgcgcgaattctaatacgactcactatag 30 DNA PAT misc_feature 1..30 note RT primer source 1..30 mol_type other DNA organism synthetic construct agtcagtcagccgaaccggaccgaagcccg 30 DNA PAT misc_feature 1..30 note Indexing primer source 1..30 mol_type other DNA organism synthetic construct cgggcttcggtccggttcggctgactgact 74 DNA PAT misc_feature 1..74 note 5HTP-II source 1..74 mol_type other DNA organism synthetic construct ggacactctgatgatcgcgtggatatggcacgcattgaattgttggacaccgtaaatgtcctaacacgtgtcca 84 DNA PAT misc_feature 1..84 note 5HTP-I aptamer source 1..84 mol_type other DNA organism synthetic construct ggagctaatgcactcttaacgccgcgtggatatgcacgcaaccgtgaatcgggcaccgtaaattccgtaagtgggtgcattagc 73 DNA PAT misc_feature 1..73 note 5HTP-II aptamer source 1..73 mol_type other DNA organism synthetic construct ggcactctgatgatcgcgtggatatggcacgcattgaattgttggacaccgtaaatgtcctaacacgggtgcc 81 DNA PAT misc_feature 1..81 note 5HTP-III aptamer source 1..81 mol_type other DNA organism synthetic construct ggagctaatgcactcccattttccgtggatatggcacgctaccgatgttgggaccgtaatgtccattacgggtgcattagc 81 DNA PAT misc_feature 1..81 note 5HTP-IV aptamer source 1..81 mol_type other DNA organism synthetic construct ggataggactctctggttcgcgtagatatggcacgcaattgaagaatgggcaccgtaaatgtctgtagacgggtcctatcc 81 DNA PAT misc_feature 1..81 note 5HTP-V aptamer source 1..81 mol_type other DNA organism synthetic construct ggataggactcattcggccgcgtggatatggcacgcaggagatgtgtggacaccgtaaatgtccgtaggcgggtcctatcc 81 DNA PAT misc_feature 1..81 note 5HTP-VI aptamer source 1..81 mol_type other DNA organism synthetic construct ggataggactcaacatctcgcgtggatatggcacgcagacttccagtgggcaccgtaaatgtccgtagacgggtcctatcc 98 DNA PAT misc_feature 1..98 note 5HTP-VII aptamer source 1..98 mol_type other DNA organism synthetic construct ggataggacatgtaatctccaaaccattcgaaagagtgggacgctagacctccggcctaaaccgaaaggtaggtagcggggctaggtatgtcctatcc 77 DNA PAT misc_feature 1..77 note 5HTP-VIII aptamer source 1..77 mol_type other DNA organism synthetic construct ggataggagctgtttggtatccaatgaaaatgtactaccaacttgaatctcccaaataggctaggtagctcctatcc 140 DNA PAT misc_feature 1..140 note 5HTP-I, SHAPE source 1..140 mol_type other DNA organism synthetic construct ggacttcggtccaagctaatgcactcttaacgccgcgtggatatgcacgcaaccgtgaatcgggcaccgtaaattccgtaagtgggtgcattagcaatcgatccggttcgccggatccaaatcgggcttcggtccggttc 142 DNA PAT misc_feature 1..142 note 5HTP-II, SHAPE source 1..142 mol_type other DNA organism synthetic construct ggacttcggtccaagctaatgcactctgatgatcgcgtggatatggcacgcattgaattgttggacaccgtaaatgtcctaacacgggtgcattagcaatcgatccggttcgccggatccaaatcgggcttcggtccggttc 139 DNA PAT misc_feature 1..139 note 5HTP-III, SHAPE source 1..139 mol_type other DNA organism synthetic construct ggacttcggtccaagctaatgcactcccattttccgtggatatggcacgctaccgatgttgggaccgtaatgtccattacgggtgcattagcaaaatcgatccggttcgccggatccaaatcgggcttcggtccggttc 116 DNA PAT misc_feature 1..116 note 5HTP-IV, SHAPE source 1..116 mol_type other DNA organism synthetic construct ggacttcggtccttggataggactctctggttcgcgtagatatggcacgcaattgaagaatgggcaccgtaaatgtctgtagacgggtcctatccaatcgggcttcggtccggttc 116 DNA PAT misc_feature 1..116 note 5HTP-V, SHAPE source 1..116 mol_type other DNA organism synthetic construct ggacttcggtccttggataggactcattcggccgcgtggatatggcacgcaggagatgtgtggacaccgtaaatgtccgtaggcgggtcctatccaatcgggcttcggtccggttc 116 DNA PAT misc_feature 1..116 note 5HTP-VI, SHAPE source 1..116 mol_type other DNA organism synthetic construct ggacttcggtccttggataggactcaacatctcgcgtggatatggcacgcagacttccagtgggcaccgtaaatgtccgtagacgggtcctatccaatcgggcttcggtccggttc 133 DNA PAT misc_feature 1..133 note 5HTP-VII, SHAPE source 1..133 mol_type other DNA organism synthetic construct ggacttcggtccttggataggacatgtaatctccaaaccattcgaaagagtgggacgctagacctccggcctaaaccgaaaggtaggtagcggggctaggtatgtcctatccaatcgggcttcggtccggttc 112 DNA PAT misc_feature 1..112 note 5HTP-VIII, SHAPE source 1..112 mol_type other DNA organism synthetic construct ggacttcggtccttggataggagctgtttggtatccaatgaaaatgtactaccaacttgaatctcccaaataggctaggtagctcctatccaatcgggcttcggtccggttc 108 DNA PAT misc_feature 1..108 note 5HTP-II/A-Broccoli source 1..108 mol_type other DNA organism synthetic construct ggacggagacggtcgggtcatatgatgatcgcgtggatatggcacgcattgaattgttggacaccgtaaatgtcctaacaatattcgagtagagtgtgggctccgtcc 108 DNA PAT misc_feature 1..108 note 5HTP-II/U-Broccoli source 1..108 mol_type other DNA organism synthetic construct ggacggagacggtcgggtctatagatgatcgcgtggatatggcacgcattgaattgttggacaccgtaaatgtcctaacatatatcgagtagagtgtgggctccgtcc 110 DNA PAT misc_feature 1..110 note 5HTP-IV/A-Broccoli source 1..110 mol_type other DNA organism synthetic construct ggacggagacggtcgggtcatattctggttcgcgtagatatggcacgcaattgaagaatgggcaccgtaaatgtctgtagacatattcgagtagagtgtgggctccgtcc 110 DNA PAT misc_feature 1..110 note 5HTP-IV/U-Broccoli source 1..110 mol_type other DNA organism synthetic construct ggacggagacggtcgggtctatatctggttcgcgtagatatggcacgcaattgaagaatgggcaccgtaaatgtctgtagactatatcgagtagagtgtgggctccgtcc 129 DNA PAT misc_feature 1..129 note 5HTP-VII/A-Broccoli source 1..129 mol_type other DNA organism synthetic construct ggacggagacggtcgggtcatatatattcgatgtaatctccaaaccattcgaaagagtgggacgctagacctccggcctaaaccgaaaggtaggtagcggggctaggtagtagagtgtgggctccgtcc 129 DNA PAT misc_feature 1..129 note 5HTP-VII/U-Broccoli source 1..129 mol_type other DNA organism synthetic construct ggacggagacggtcgggtctatatgtaatctccaaaccattcgaaagagtgggacgctagacctccggcctaaaccgaaaggtaggtagcggggctaggtatatatcgagtagagtgtgggctccgtcc 108 DNA PAT misc_feature 1..108 note 5HTP-VIII/A-Broccoli source 1..108 mol_type other DNA organism synthetic construct ggacggagacggtcgggtcatattgtttggtatccaatgaaaatgtactaccaacttgaatctcccaaataggctaggtaatattcgagtagagtgtgggctccgtcc 108 DNA PAT misc_feature 1..108 note 5HTP-VIII/U-Broccoli source 1..108 mol_type other DNA organism synthetic construct ggacggagacggtcgggtctatatgtttggtatccaatgaaaatgtactaccaacttgaatctcccaaataggctaggtatatatcgagtagagtgtgggctccgtcc 221 DNA PAT misc_feature 1..221 note 5HTP-IV/pbuE riboswitch source 1..221 mol_type other DNA organism synthetic construct aatattgagctgttgacaattaatcatcggctcgtataatgtgtggaattaaatagctattatcaggatttttctggttcgcgtagatatggcacgcaattgaagaatgggcaccgtaaatgtctgtagacaaaatcctgattacaaaatttgtttatgacattttttgtaatcaggatttttttatttatcaaaacatttaagtaaaggagtttgttatg 52 DNA PAT misc_feature 1..52 note Outer primer source 1..52 mol_type other DNA organism synthetic construct ggccgtaatacgactcactataggagcccggatagctcagtcggtagagcag 30 DNA PAT misc_feature 1..30 note Outer primer source 1..30 mol_type other DNA organism synthetic construct tggcgcccgaacagggacttgaaccctgga 61 RNA PAT misc_feature 1..61 note GR scaffold is derived from the aptamer domain of the B. subtilisxpt-pbuX guanine riboswitch source 1..61 mol_type other RNA organism synthetic construct catataatcgcgtggatatggcacgcaagtttctaccgggcaccgtaaatgtccgactatg 82 RNA PAT misc_feature 1..82 note CDG scaffold derived from the aptamer domain V. cholera Vc2cyclic di-GMP riboswitch (PDB ID 3IWN) source 1..82 mol_type other RNA organism synthetic construct cacgcacagggcaaaccattcgaaagagtgggacgcaaagcctccggcctaaaccgaaaggtaggtagcggggttaccgatg 62 RNA PAT misc_feature 1..62 note HH scaffold derived from the S. mansoni hammerhead ribozyme (PDBID 3ZP8) source 1..62 mol_type other RNA organism synthetic construct gcgtcctggtatccaatgaaaatgtactaccagctgatgagtcccaaataggacgaaaacgc 63 RNA PAT misc_feature 1..63 note 5HTP-I source 1..63 mol_type other RNA organism synthetic construct ctcttaacgccgcgtggatatgcacgcaaccgtgaatcgggcaccgtaaattccgtaagtggg 65 RNA PAT misc_feature 1..65 note 5HTP-II source 1..65 mol_type other RNA organism synthetic construct ctctgatgatcgcgtggatatggcacgcattgaattgttggrcaccgtaaatgtcctaacacggg 15 RNA PAT misc_feature 1..15 note 5HTP-III source 1..15 mol_type other RNA organism synthetic construct ggatatggcacgcta 13 RNA PAT misc_feature 1..13 note 5HTP-III(Trp aptamer) source 1..13 mol_type other RNA organism synthetic construct gaccgtaatgtcc 63 RNA PAT misc_feature 1..63 note 5HTP-IV misc_feature 3..4 note n is a, c, g, or u misc_feature 6..9 note n is a, c, g, or u source 1..63 mol_type other RNA organism synthetic construct tcnnynnnncgcgtrgatatggcacgcaattgaagaatgggcaccgtaaatgtctgtagacgg 63 RNA PAT misc_feature 1..63 note 5HTP-V misc_feature 4 note n is a, c, g, or u misc_feature 6 note n is a, c, g, or u source 1..63 mol_type other RNA organism synthetic construct tcrnynrrycgcgtggatatggcacgcaggagatgtgtggacaccgtaaatgtccgtaggcgg 63 RNA PAT misc_feature 1..63 note 5HTP-VI misc_feature 6..8 note n is a, c, g, or u misc_feature 29..32 note n is a, c, g, or u misc_feature 37 note n is a, c, g, or u source 1..63 mol_type other RNA organism synthetic construct tcaacnnnycgcgtggatatggcacgcannnntcyantgggcaccgtaaatgtccgtagacgg 139 RNA PAT misc_feature 1..139 note Secondary structure of an artificial 5HTP/serotonin "ON"riboswitch based upon 5HTP-IV aptamer source 1..139 mol_type other RNA organism synthetic construct aattaaatagctattatcaggatttttctggttcgcgtagatatggcacgcaattgaagaatgggcaccgtaaatgtctgtagacaaaatcctgattacaaaatttgtttatgacattttttgtaatcaggattttttt 82 RNA PAT misc_feature 1..82 note 5HTP-VII misc_feature 38..40 note n is a, c, g, or u misc_feature 71 note n is a, c, g, or u source 1..82 mol_type other RNA organism synthetic construct catgtaatctccaaaccattcgaaagagtgggacgyynnncctccggcctaaaccgaaaggtaggtagcgnggcyaggtrtg 61 RNA PAT misc_feature 1..61 note 5HTP-VIII misc_feature 33 note n is a, c, g, or u misc_feature 35..37 note n is a, c, g, or u source 1..61 mol_type other RNA organism synthetic construct gctgtytggtatccaatgaaaatgtactaccanynnnratctcccaaataggctaggtrgc 61 RNA PAT misc_feature 1..61 note 5HTP-VIII misc_feature 33 note n is a, c, g, or u misc_feature 35..37 note n is a, c, g, or u source 1..61 mol_type other RNA organism synthetic construct gctgtytggtatccaatgaaaatgtactaccanynnnratctcccaaataggctaggtrgc 56 RNA PAT misc_feature 1..56 note 5HTP-II-Broccoli biosensors source 1..56 mol_type other RNA organism synthetic construct gatgatcgcgtggatatggcacgcttgaattgttggacaccgtaaatgtcctaaca 58 RNA PAT misc_feature 1..58 note 5HTP-IV -Broccoli biosensors source 1..58 mol_type other RNA organism synthetic construct tctggttcgcgtagatatggcacgcaattgaagaatgggcaccgtaaatgtctgtaga 78 RNA PAT misc_feature 1..78 note 5HTP-VII-Broccoli biosensors source 1..78 mol_type other RNA organism synthetic construct tgtaatctccaaaccattcgaaagagtgggacgctagacctccggcctaaaccgaaaggtaggtagcggggctaggta 57 RNA PAT misc_feature 1..57 note 5HTP-VIII -Broccoli biosensors source 1..57 mol_type other RNA organism synthetic construct tgtttggtatccaatgaaaatgtactaccaacttgaatctcccaaataggctaggta 19 RNA PAT misc_feature 1..19 note Broccoli source 1..19 mol_type other RNA organism synthetic construct ggacggagacggtcgggtc 24 RNA PAT misc_feature 1..24 note Broccoli source 1..24 mol_type other RNA organism synthetic construct tcgagtagagtgtgggctccgtcc 120 RNA PAT misc_feature 1..120 note Guanine riboswitch aptamer (GR) RNA library for the selectionusing the SuperScript III RT misc_feature 27..33 note n is a, c, g, or u misc_feature 53..62 note n is a, c, g, or u misc_feature 80..85 note n is a, c, g, or u source 1..120 mol_type other RNA organism synthetic construct ggacttcggtccaagctaatgcactcnnnnnnncgcgtggatatggcacgcannnnnnnnnngggcaccgtaaatgtccnnnnnngggtgcattagcaaaatcgggcttcggtccggttc 118 RNA PAT misc_feature 1..118 note GR RNA library used for the selection using the GsI-IIC RT misc_feature 26..32 note n is a, c, g, or u misc_feature 52..61 note n is a, c, g, or u misc_feature 79..84 note n is a, c, g, or u source 1..118 mol_type other RNA organism synthetic construct ggacttcggtccttggataggactcnnnnnnncgcgtggatatggcacgcannnnnnnnnngggcaccgtaaatgtccnnnnnngggtcctatccccaatcgggcttcggtccggttc 135 RNA PAT misc_feature 1..135 note di-cyclic GMP riboswitch aptamer (CG misc_feature 25..33 note n is a, c, g, or u misc_feature 58..62 note n is a, c, g, or u misc_feature 96..102 note n is a, c, g, or u source 1..135 mol_type other RNA organism synthetic construct ggacttcggtccttggataggacannnnnnnnncaaaccattcgaaagagtgggacgnnnnncctccggcctaaaccgaaaggtaggtagcggggnnnnnnntgtcctatccccaatcgggcttcggtccggttc 115 RNA PAT misc_feature 1..115 note Hammerhead ribozyme (HR) library misc_feature 25..28 note n is a, c, g, or u misc_feature 55..64 note n is a, c, g, or u misc_feature 75..82 note n is a, c, g, or u source 1..115 mol_type other RNA organism synthetic construct ggacttcggtccttggataggagcnnnntggtatccaatgaaaatgtactaccannnnnnnnnncccaaataggnnnnnnnngctcctatccccaatcgggcttcggtccggttc 82 RNA PAT misc_feature 1..82 note DGR-I misc_feature 40 note n is a, c, g, or u source 1..82 mol_type other RNA organism synthetic construct ggataggactccgtaattcgcgtggatatggcacgcagtnaggtgttgggcaccgtaaatgcyccayrtagctatccccaat 80 RNA PAT misc_feature 1..80 note DGR-II source 1..80 mol_type other RNA organism synthetic construct ggataggactcgytaagacgcgtggatatggcacgcaattaagtgttgggcaccgtaaatgtcctccggctayccccaat 86 RNA PAT misc_feature 1..86 note DGR-III misc_feature 38..43 note n is a, c, g, or u misc_feature 69 note n is a, c, g, or u source 1..86 mol_type other RNA organism synthetic construct ggataggactcaggtgggcgcgtggatatggcacgcannnnnngtgggggcaccgtaaatgtccgagtnrggrtcctatccccaat 86 RNA PAT misc_feature 1..86 note DGR-IV misc_feature 38..42 note n is a, c, g, or u misc_feature 75 note n is a, c, g, or u source 1..86 mol_type other RNA organism synthetic construct ggataggactcaggtggtcgcgtggatatggcacgcannnnnrrggtgggcaccgtaaatgtccgggtrrgggtnctatccccaat 74 DNA PAT misc_feature 1..74 note 5GR-II source 1..74 mol_type other DNA organism synthetic construct ggacactctgatgatcgcgtggatatggcacgcattgaattgttggacaccgtaaatgtcctaacacgtgtcca 84 DNA PAT misc_feature 1..84 note 5GR-I aptamer source 1..84 mol_type other DNA organism synthetic construct ggagctaatgcactcttaacgccgcgtggatatgcacgcaaccgtgaatcgggcaccgtaaattccgtaagtgggtgcattagc 73 DNA PAT misc_feature 1..73 note 5GR-II aptamer source 1..73 mol_type other DNA organism synthetic construct ggcactctgatgatcgcgtggatatggcacgcattgaattgttggacaccgtaaatgtcctaacacgggtgcc 81 DNA PAT misc_feature 1..81 note 5GR-III aptamer source 1..81 mol_type other DNA organism synthetic construct ggagctaatgcactcccattttccgtggatatggcacgctaccgatgttgggaccgtaatgtccattacgggtgcattagc 81 DNA PAT misc_feature 1..81 note 5GR-IV aptamer source 1..81 mol_type other DNA organism synthetic construct ggataggactctctggttcgcgtagatatggcacgcaattgaagaatgggcaccgtaaatgtctgtagacgggtcctatcc 81 DNA PAT misc_feature 1..81 note 5GR-V aptamer source 1..81 mol_type other DNA organism synthetic construct ggataggactcattcggccgcgtggatatggcacgcaggagatgtgtggacaccgtaaatgtccgtaggcgggtcctatcc 81 DNA PAT misc_feature 1..81 note 5GR-VI aptamer source 1..81 mol_type other DNA organism synthetic construct ggataggactcaacatctcgcgtggatatggcacgcagacttccagtgggcaccgtaaatgtccgtagacgggtcctatcc 98 DNA PAT misc_feature 1..98 note 5GR-VII aptamer source 1..98 mol_type other DNA organism synthetic construct ggataggacatgtaatctccaaaccattcgaaagagtgggacgctagacctccggcctaaaccgaaaggtaggtagcggggctaggtatgtcctatcc 77 DNA PAT misc_feature 1..77 note 5GR-VIII aptamer source 1..77 mol_type other DNA organism synthetic construct ggataggagctgtttggtatccaatgaaaatgtactaccaacttgaatctcccaaataggctaggtagctcctatcc 140 DNA PAT misc_feature 1..140 note 5GR-I, SHAPE source 1..140 mol_type other DNA organism synthetic construct ggacttcggtccaagctaatgcactcttaacgccgcgtggatatgcacgcaaccgtgaatcgggcaccgtaaattccgtaagtgggtgcattagcaatcgatccggttcgccggatccaaatcgggcttcggtccggttc 142 DNA PAT misc_feature 1..142 note 5GR-II, SHAPE source 1..142 mol_type other DNA organism synthetic construct ggacttcggtccaagctaatgcactctgatgatcgcgtggatatggcacgcattgaattgttggacaccgtaaatgtcctaacacgggtgcattagcaatcgatccggttcgccggatccaaatcgggcttcggtccggttc 139 DNA PAT misc_feature 1..139 note 5GR-III, SHAPE source 1..139 mol_type other DNA organism synthetic construct ggacttcggtccaagctaatgcactcccattttccgtggatatggcacgctaccgatgttgggaccgtaatgtccattacgggtgcattagcaaaatcgatccggttcgccggatccaaatcgggcttcggtccggttc 116 DNA PAT misc_feature 1..116 note 5GR-IV, SHAPE source 1..116 mol_type other DNA organism synthetic construct ggacttcggtccttggataggactctctggttcgcgtagatatggcacgcaattgaagaatgggcaccgtaaatgtctgtagacgggtcctatccaatcgggcttcggtccggttc 116 DNA PAT misc_feature 1..116 note 5GR-V, SHAPE source 1..116 mol_type other DNA organism synthetic construct ggacttcggtccttggataggactcattcggccgcgtggatatggcacgcaggagatgtgtggacaccgtaaatgtccgtaggcgggtcctatccaatcgggcttcggtccggttc 116 DNA PAT misc_feature 1..116 note 5GR-VI, SHAPE source 1..116 mol_type other DNA organism synthetic construct ggacttcggtccttggataggactcaacatctcgcgtggatatggcacgcagacttccagtgggcaccgtaaatgtccgtagacgggtcctatccaatcgggcttcggtccggttc 133 DNA PAT misc_feature 1..133 note 5CG-I, SHAPE source 1..133 mol_type other DNA organism synthetic construct ggacttcggtccttggataggacatgtaatctccaaaccattcgaaagagtgggacgctagacctccggcctaaaccgaaaggtaggtagcggggctaggtatgtcctatccaatcgggcttcggtccggttc 112 DNA PAT misc_feature 1..112 note 5HR-I, SHAPE source 1..112 mol_type other DNA organism synthetic construct ggacttcggtccttggataggagctgtttggtatccaatgaaaatgtactaccaacttgaatctcccaaataggctaggtagctcctatccaatcgggcttcggtccggttc 159 DNA PAT misc_feature 1..159 note 5GR-II/CM-2 source 1..159 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctagatgatcgcgtggatatggcacgcattgaattgttggacaccgtaaatgtcctaacatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 161 DNA PAT misc_feature 1..161 note 5GR-II/CM-3 source 1..161 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagcgcagcggagacggtcgggtctatgatgatcgcgtggatatggcacgcattgaattgttggacaccgtaaatgtcctaacaatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 163 DNA PAT misc_feature 1..163 note 5GR-II/CM-4 source 1..163 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctatagctgatcgcgtggatatggcacgcattgaattgttggacaccgtaaatgtcctaacatatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 165 DNA PAT misc_feature 1..165 note 5GR-II/CM-5 source 1..165 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctatatgatgatcgcgtggatatggcacgcattgaattgttggacaccgtaaatgtcctaacaatatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 161 DNA PAT misc_feature 1..161 note 5GR-IV/CM-2 source 1..161 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctatctggttcgcgtagatatggcacgcaattgaagaatgggcaccgtaaatgtctgtagactatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 163 DNA PAT misc_feature 1..163 note 5GR-IV/CM-3 source 1..163 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctattctggttcgcgtagatatggcacgcaattgaagaatgggcaccgtaaatgtctgtagacatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 165 DNA PAT misc_feature 1..165 note 5GR-IV/CM-4 source 1..165 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctatatctggttcgcgtagatatggcacgcaattgaagaatgggcaccgtaaatgtctgtagactatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 167 DNA PAT misc_feature 1..167 note 5GR-IV/CM-5 source 1..167 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctatattctggttcgcgtagatatggcacgcaattgaagaatgggcaccgtaaatgtctgtagacatatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 161 DNA PAT misc_feature 1..161 note 5GR-V/CM-2 source 1..161 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctaattcggccgcgtggatatggcacgcaggagatgtgtggacaccgtaaatgtccgtaggctatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 163 DNA PAT misc_feature 1..163 note 5GR-V/CM-3 source 1..163 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctatattcggccgcgtggatatggcacgcaggagatgtgtggacaccgtaaatgtccgtaggcatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 165 DNA PAT misc_feature 1..165 note 5GR-V/CM-4 source 1..165 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctataattcggccgcgtggatatggcacgcaggagatgtgtggacaccgtaaatgtccgtaggctatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 167 DNA PAT misc_feature 1..167 note 5GR-V/CM-5 source 1..167 mol_type other DNA organism synthetic construct gcccggatagctcagtccgtagagcagcggagacggtcgggtctatatattcggccgcgtggatatggcacgcaggagatgtgtggacaccgtaaatgtccgtaggcatatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 161 DNA PAT misc_feature 1..161 note 5GR-VI/CM-2 source 1..161 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctaaacatctcgcgtggatatggcacgcagacttccagtgggcaccgtaaatgtccgtagactatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 163 DNA PAT misc_feature 1..163 note DGR-VI/CM-3 source 1..163 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctataacatctcgcgtggatatggcacgcagacttccagtgggcaccgtaaatgtccgtagacatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 165 DNA PAT misc_feature 1..165 note DGR-VI/CM-4 source 1..165 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctataaacatctcgcgtggatatggcacgcagacttccagtgggcaccgtaaatgtccgtagactatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 167 DNA PAT misc_feature 1..167 note DGR-VI/CM-5 source 1..167 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctatataacatctcgcgtggatatggcacgcagacttccagtgggcaccgtaaatgtccgtagacatatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 167 DNA PAT misc_feature 1..167 note DGR-I/CM-2 source 1..167 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctagactccgtaattcgcgtggatatggcacgcagtcaggtgttgggcaccgtaaatgtcccacatagtatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 169 DNA PAT misc_feature 1..169 note DGR-I/CM-3 source 1..169 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctatgactccgtaattcgcgtggatatggcacgcagtcaggtgttgggcaccgtaaatgtcccacatagatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 171 DNA PAT misc_feature 1..171 note DGR-I/CM-4 source 1..171 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctatagactccgtaattcgcgtggatatggcacgcagtcaggtgttgggcaccgtaaatgtcccacatagtatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 173 DNA PAT misc_feature 1..173 note DGR-I/CM-5 source 1..173 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctatatgactccgtaattcgcgtggatatggcacgcagtcaggtgttgggcaccgtaaatgtcccacatagatatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 166 DNA PAT misc_feature 1..166 note DGR-II/CM-2 source 1..166 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctagactccgtaattcgcgtggatatggcacgcagtaaggtattgggcaccgtaaatgtccatatgttatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 168 DNA PAT misc_feature 1..168 note DGR-II/CM-3 source 1..168 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctatgactccgtaattcgcgtggatatggcacgcagtaaggtattgggcaccgtaaatgtccatatgtatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 170 DNA PAT misc_feature 1..170 note DGR-II/CM-4 source 1..170 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctatagactccgtaattcgcgtggatatggcacgcagtaaggtattgggcaccgtaaatgtccatatgttatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 172 DNA PAT misc_feature 1..172 note DGR-II/CM-5 source 1..172 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctatatgactccgtaattcgcgtggatatggcacgcagtaaggtattgggcaccgtaaatgtccatatgtatatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 161 DNA PAT misc_feature 1..161 note DGR-III/CM-2 source 1..161 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctaaggtgggcgcgtggatatggcacgcatgtttggtgggggcaccgtaaatgtccgagttatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 163 DNA PAT misc_feature 1..163 note DGR-III/CM-3 source 1..163 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctataggtgggcgcgtggatatggcacgcatgtttggtgggggcaccgtaaatgtccgagttaatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 165 DNA PAT misc_feature 1..165 note DGR-III/CM-4 source 1..165 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctataaggtgggcgcgtggatatggcacgcatgtttggtgggggcaccgtaaatgtccgagttatatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 167 DNA PAT misc_feature 1..167 note DGR-III/CM-5 source 1..167 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctatataggtgggcgcgtggatatggcacgcatgtttggtgggggcaccgtaaatgtccgagttaatatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 161 DNA PAT misc_feature 1..161 note DGR-IV/CM-2 source 1..161 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctaaggtggtcgcgtggatatggcacgcataatagaggtgggcaccgtaaatgtccgggtaatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 163 DNA PAT misc_feature 1..163 note DGR-IV/CM-3 source 1..163 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctataggtggtcgcgtggatatggcacgcataatagaggtgggcaccgtaaatgtccgggtaaatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 165 DNA PAT misc_feature 1..165 note DGR-IV/CM-4 source 1..165 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctataaggtggtcgcgtggatatggcacgcataatagaggtgggcaccgtaaatgtccgggtaatatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca 167 DNA PAT misc_feature 1..167 note DGR-IV/CM-5 source 1..167 mol_type other DNA organism synthetic construct gcccggatagctcagtcggtagagcagcggagacggtcgggtctatataggtggtcgcgtggatatggcacgcataatagaggtgggcaccgtaaatgtccgggtaaatatatcgagtagagtgtgggctccgcgggtccagggttcaagtccctgttcgggcgcca