NI2023TC1257 CN 202210825092.1 2022-07-13 HANGZHOU ZHONGMEIHUADONG PHARMACEUTICAL CO., LTD. HANGZHOU ZHONGMEIHUADONG PHARMACEUTICAL CO., LTD. GLP-1/GIP DUAL AGONIST, PREPARATION METHOD AND USE THEREOF 13 40 AA PAT source 1..40 mol_type protein organism synthetic construct MOD_RES 2 note Aib MOD_RES 6 note alpha methyl phenylalanine MOD_RES 11 note Aib MOD_RES 13 note Aib MOD_RES 40 note amidated YXEGTXTSDYXIXLDKQAQAEFVKWLLKGGPSSGAPPPSK 40 AA PAT source 1..40 mol_type protein organism synthetic construct MOD_RES 2 note Aib MOD_RES 6 note alpha methyl phenylalanine MOD_RES 11 note Aib MOD_RES 13 note Aib MOD_RES 40 note amidated SITE 24 note the epsilon-amino of the K side-chain is conjugated with ([2-(2-amino-ethoxy)-ethoxy]-acetyl)2-(gama-Glu)1-CO-(CH2)16-CO2H YXEGTXTSDYXIXLDKQAQAEFVKWLLKGGPSSGAPPPSK 40 AA PAT source 1..40 mol_type protein organism synthetic construct MOD_RES 2 note Aib MOD_RES 6 note alpha methyl phenylalanine MOD_RES 11 note Aib MOD_RES 13 note Aib MOD_RES 40 note amidated SITE 28 note the epsilon-amino of the K side-chain is conjugated with ([2-(2-amino-ethoxy)-ethoxy]-acetyl)2-(gama-Glu)1-CO-(CH2)16-CO2H YXEGTXTSDYXIXLDKQAQAEFVKWLLKGGPSSGAPPPSK 40 AA PAT source 1..40 mol_type protein organism synthetic construct MOD_RES 2 note Aib MOD_RES 6 note alpha methyl phenylalanine MOD_RES 11 note Aib MOD_RES 13 note Aib MOD_RES 40 note amidated SITE 24 note the epsilon-amino of the K side-chain is conjugatged with ([2-(2-amino-ethoxy)-ethoxy]-acetyl)2-(gama-Glu)1-CO-(CH2)18-CO2H YXEGTXTSDYXIXLDKQAQAEFVKWLLKGGPSSGAPPPSK 40 AA PAT source 1..40 mol_type protein organism synthetic construct MOD_RES 2 note Aib MOD_RES 6 note alpha methyl phenylalanine MOD_RES 11 note Aib MOD_RES 13 note Aib MOD_RES 40 note amidated SITE 24 note the epsilon-amino of the K side-chain with ([2-(2-amino-ethoxy)-ethoxy]-acetyl)2-(gama-Glu)1-CO-(CH2)20-CO2H YXEGTXTSDYXIXLDKQAQAEFVKWLLKGGPSSGAPPPSK 40 AA PAT source 1..40 mol_type protein organism synthetic construct MOD_RES 2 note Aib MOD_RES 6 note alpha methyl phenylalanine MOD_RES 11 note Aib MOD_RES 13 note Aib MOD_RES 40 note amidated SITE 24 note the epsilon-amino of the K side-chain with ([2-(2-amino-ethoxy)-ethoxy]-acetyl)2-(gama-Glu)3-CO-(CH2)16-CO2H YXEGTXTSDYXIXLDKQAQAEFVKWLLKGGPSSGAPPPSK 40 AA PAT source 1..40 mol_type protein organism synthetic construct MOD_RES 2 note Aib MOD_RES 6 note alpha methyl phenylalanine MOD_RES 11 note Aib MOD_RES 13 note Aib MOD_RES 40 note amidated SITE 24 note the epsilon-amino of the K side-chain with ([2-(2-amino-ethoxy)-ethoxy]-acetyl)3-(gama-Glu)1-CO-(CH2)20-CO2H YXEGTXTSDYXIXLDKQAQAEFVKWLLKGGPSSGAPPPSK 40 AA PAT source 1..40 mol_type protein organism synthetic construct MOD_RES 2 note Aib MOD_RES 6 note alpha methyl phenylalanine MOD_RES 11 note Aib MOD_RES 13 note Aib MOD_RES 40 note amidated SITE 28 note the epsilon-amino of the K side-chain with ([2-(2-amino-ethoxy)-ethoxy]-acetyl)2-(gama-Glu)1-CO-(CH2)20-CO2H YXEGTXTSDYXIXLDKQAQAEFVKWLLKGGPSSGAPPPSK 40 AA PAT source 1..40 mol_type protein organism synthetic construct MOD_RES 2 note Aib MOD_RES 6 note alpha methyl phenylalanine MOD_RES 11 note Aib MOD_RES 13 note Aib MOD_RES 40 note amidated SITE 28 note the epsilon-amino of the K side-chain with ([2-(2-amino-ethoxy)-ethoxy]-acetyl)2-(gama-Glu)3-CO-(CH2)16-CO2H YXEGTXTSDYXIXLDKQAQAEFVKWLLKGGPSSGAPPPSK 40 AA PAT source 1..40 mol_type protein organism synthetic construct MOD_RES 2 note Aib MOD_RES 6 note alpha methyl phenylalanine MOD_RES 11 note Aib MOD_RES 13 note Aib MOD_RES 40 note amidated SITE 28 note the epsilon-amino of the K side-chain with ([2-(2-amino-ethoxy)-ethoxy]-acetyl)3-(gama-Glu)1-CO-(CH2)16-CO2H YXEGTXTSDYXIXLDKQAQAEFVKWLLKGGPSSGAPPPSK 40 AA PAT source 1..40 mol_type protein organism synthetic construct MOD_RES 2 note Aib MOD_RES 6 note alpha methyl phenylalanine MOD_RES 11 note Aib MOD_RES 13 note Aib MOD_RES 40 note amidated SITE 28 note the epsilon-amino of the K side-chain with ([2-(2-amino-ethoxy)-ethoxy]-acetyl)3-(gama-Glu)1-CO-(CH2)20-CO2H YXEGTXTSDYXIXLDKQAQAEFVKWLLKGGPSSGAPPPSK 40 AA PAT source 1..40 mol_type protein organism synthetic construct MOD_RES 2 note Aib MOD_RES 6 note alpha methyl phenylalanine MOD_RES 11 note Aib MOD_RES 13 note Aib MOD_RES 40 note amidated SITE 24 note the epsilon-amino of the K side-chain may be conjugated with ([2-(2-amino-ethoxy)-ethoxy]-acetyl)a-(gama-Glu)b-CO-(CH2)c-Z, wherein each a is independently an integer from 0 to 5, each b is independently an integer from 0 to 5, and each c is independently an integer from 10 to 24, wherein Z is independently selected from the group consisting of -CH3, carboxylic acids or carboxylic acid bioisosteres, phosphonates or sulfonates SITE 16 note the epsilon-amino of the K side-chain may be conjugated with ([2-(2-amino-ethoxy)-ethoxy]-acetyl)a-(gama-Glu)b-CO-(CH2)c-Z, wherein each a is independently an integer from 0 to 5, each b is independently an integer from 0 to 5, and each c is independently an integer from 10 to 24, wherein Z is independently selected from the group consisting of -CH3, carboxylic acids or carboxylic acid bioisosteres, phosphonates or sulfonates SITE 28 note the epsilon-amino of the K side-chain may be conjugated with ([2-(2-amino-ethoxy)-ethoxy]-acetyl)a-(gama-Glu)b-CO-(CH2)c-Z, wherein each a is independently an integer from 0 to 5, each b is independently an integer from 0 to 5, and each c is independently an integer from 10 to 24, wherein Z is independently selected from the group consisting of -CH3, carboxylic acids or carboxylic acid bioisosteres, phosphonates or sulfonates SITE 40 note the epsilon-amino of the K side-chain may be conjugated with ([2-(2-amino-ethoxy)-ethoxy]-acetyl)a-(gama-Glu)b-CO-(CH2)c-Z, wherein each a is independently an integer from 0 to 5, each b is independently an integer from 0 to 5, and each c is independently an integer from 10 to 24, wherein Z is independently selected from the group consisting of -CH3, carboxylic acids or carboxylic acid bioisosteres, phosphonates or sulfonates YXEGTXTSDYXIXLDKQAQAEFVKWLLKGGPSSGAPPPSK 40 AA PAT source 1..40 mol_type protein organism synthetic construct MOD_RES 2 note Aib MOD_RES 6 note alpha methyl phenylalanine MOD_RES 11 note Aib or Ala MOD_RES 13 note Aib, cyclohexylalanine, Leu, alpha methyl phenylalanine or alpha methyl tyrosine SITE 24 note the epsilon-amino of the K side-chain may be conjugated with ([2-(2-amino-ethoxy)-ethoxy]-acetyl)a-(gama-Glu)b-CO-(CH2)c-Z, wherein each a is independently an integer from 0 to 5, each b is independently an integer from 0 to 5, and each c is independently an integer from 10 to 24, wherein Z is independently selected from the group consisting of -CH3, carboxylic acids or carboxylic acid bioisosteres, phosphonates or sulfonates MOD_RES 12 note Ala, Ile, Lys, Phe or 4-pyridinyl alanine MOD_RES 17 note Gln or Ile MOD_RES 20 note Ala or Ser MOD_RES 29 note Gln or Gly SITE 16 note the epsilon-amino of the K side-chain may be conjugated with ([2-(2-amino-ethoxy)-ethoxy]-acetyl)a-(gama-Glu)b-CO-(CH2)c-Z, wherein each a is independently an integer from 0 to 5, each b is independently an integer from 0 to 5, and each c is independently an integer from 10 to 24, wherein Z is independently selected from the group consisting of -CH3, carboxylic acids or carboxylic acid bioisosteres, phosphonates or sulfonates SITE 28 note the epsilon-amino of the K side-chain may be conjugated with ([2-(2-amino-ethoxy)-ethoxy]-acetyl)a-(gama-Glu)b-CO-(CH2)c-Z, wherein each a is independently an integer from 0 to 5, each b is independently an integer from 0 to 5, and each c is independently an integer from 10 to 24, wherein Z is independently selected from the group consisting of -CH3, carboxylic acids or carboxylic acid bioisosteres, phosphonates or sulfonates SITE 40 note the epsilon-amino of the K side-chain may be conjugated with ([2-(2-amino-ethoxy)-ethoxy]-acetyl)a-(gama-Glu)b-CO-(CH2)c-Z, wherein each a is independently an integer from 0 to 5, each b is independently an integer from 0 to 5, and each c is independently an integer from 10 to 24, wherein Z is independently selected from the group consisting of -CH3, carboxylic acids or carboxylic acid bioisosteres, phosphonates or sulfonates YXEGTXTSDYXXXLDKXAQXEFVKWLLKXGPSSGAPPPSK